##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031645_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2377132 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.40907867127278 34.0 31.0 34.0 31.0 34.0 2 32.632991773279734 34.0 31.0 34.0 31.0 34.0 3 32.72507795107718 34.0 31.0 34.0 31.0 34.0 4 36.19888125690959 37.0 35.0 37.0 35.0 37.0 5 35.94944454073228 37.0 35.0 37.0 35.0 37.0 6 36.025411293945815 37.0 36.0 37.0 35.0 37.0 7 35.975268937526394 37.0 36.0 37.0 35.0 37.0 8 36.06713930904973 37.0 36.0 37.0 35.0 37.0 9 37.90537799331295 39.0 38.0 39.0 35.0 39.0 10-11 37.883578194227326 39.0 38.0 39.0 35.0 39.0 12-13 37.91935260641816 39.0 38.0 39.0 35.0 39.0 14-15 39.317031616250176 41.0 39.0 41.0 36.0 41.0 16-17 39.267920544589025 41.0 39.0 41.0 36.0 41.0 18-19 39.35969941930023 41.0 39.0 41.0 36.0 41.0 20-21 39.419203266793765 41.0 39.0 41.0 36.0 41.0 22-23 39.33108279220506 41.0 39.0 41.0 36.0 41.0 24-25 39.24825861584464 41.0 39.0 41.0 36.0 41.0 26-27 39.1111225628194 41.0 39.0 41.0 36.0 41.0 28-29 39.01547326778656 40.5 39.0 41.0 35.0 41.0 30-31 38.88822139452079 40.0 39.0 41.0 35.0 41.0 32-33 38.76262719108573 40.0 38.0 41.0 35.0 41.0 34-35 38.67774822769623 40.0 38.0 41.0 35.0 41.0 36-37 38.49865573304301 40.0 38.0 41.0 34.0 41.0 38-39 38.283770316499044 40.0 38.0 41.0 34.0 41.0 40-41 38.24990618947538 40.0 38.0 41.0 34.0 41.0 42-43 38.1679511276614 40.0 38.0 41.0 34.0 41.0 44-45 38.14230572807905 40.0 38.0 41.0 33.5 41.0 46-47 38.169529289917435 40.0 38.0 41.0 33.5 41.0 48-49 38.312720959542844 40.0 38.5 41.0 34.0 41.0 50-51 38.23146947666348 40.0 38.0 41.0 33.0 41.0 52-53 38.2223664903758 40.0 38.0 41.0 34.0 41.0 54-55 38.16916161996893 40.0 38.0 41.0 34.0 41.0 56-57 38.07721468559592 40.0 38.0 41.0 33.0 41.0 58-59 37.91412929530207 40.0 38.0 41.0 33.0 41.0 60-61 37.63076030275138 40.0 38.0 41.0 32.0 41.0 62-63 37.42653268728871 40.0 37.0 41.0 32.0 41.0 64-65 37.148697884677844 39.5 37.0 41.0 31.0 41.0 66-67 36.75671502465997 39.0 36.0 41.0 30.5 41.0 68-69 36.24622633492797 39.0 36.0 41.0 29.5 41.0 70-71 35.765983336221964 38.0 35.0 40.0 29.0 41.0 72-73 35.35038315078843 37.0 35.0 39.5 28.5 41.0 74-75 34.90380698253189 37.0 35.0 39.0 28.0 41.0 76-77 34.21699846706031 36.0 34.0 39.0 27.5 40.0 78-79 34.0933894289421 36.0 34.0 37.5 28.0 39.5 80-81 33.71451711558298 35.5 34.0 37.0 27.0 39.0 82-83 33.34792956386099 35.0 34.0 37.0 26.5 39.0 84-85 33.04086079359497 35.0 34.0 36.0 26.0 37.5 86-87 32.77747470481235 35.0 34.0 36.0 26.0 37.0 88-89 32.53955817346281 35.0 34.0 35.5 26.0 37.0 90-91 32.35962432881304 35.0 33.5 35.0 25.0 36.0 92-93 32.22202532295219 35.0 33.0 35.0 25.0 36.0 94-95 32.08558822143659 35.0 33.0 35.0 25.0 36.0 96-97 31.989856894779088 35.0 33.0 35.0 25.0 35.5 98-99 31.905254314863456 35.0 33.0 35.0 24.5 35.0 100-101 31.498155340132563 34.5 32.5 35.0 22.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16138711504461156 1101 2 0.24540184211408445 1101 3 0.2187143336095545 1101 4 0.18573688246677023 1101 5 0.2562530752915606 1101 6 0.1748333425680002 1101 7 0.34165783394082183 1101 8 0.2337450256195197 1101 9 0.26299202078262596 1101 10-11 0.21849976342721789 1101 12-13 0.19888253745603635 1101 14-15 0.23025982100509168 1101 16-17 0.32917141824702867 1101 18-19 0.23908679541325029 1101 20-21 0.17119656030170205 1101 22-23 0.19751743897179352 1101 24-25 0.16875435593736654 1101 26-27 0.21241297473736864 1101 28-29 0.2349976581741089 1101 30-31 0.06270049407144995 1101 32-33 0.13330789077114247 1101 34-35 0.1202407552858702 1101 36-37 0.066593572564976 1101 38-39 0.15003055311835567 1101 40-41 0.2597525053435348 1101 42-43 0.08279001289856325 1101 44-45 0.3311641291896592 1101 46-47 0.37762145575306505 1101 48-49 0.18096790730293577 1101 50-51 0.4021819297063729 1101 52-53 0.18585710530442867 1101 54-55 0.43330492607501014 1101 56-57 0.45658219913521236 1101 58-59 0.31272789688166114 1101 60-61 0.23082684099714612 1101 62-63 0.18001913576828343 1101 64-65 0.3586331793668336 1101 66-67 0.34396570005051075 1101 68-69 0.3378299399707245 1101 70-71 0.3885385867328708 1101 72-73 0.3913912619688773 1101 74-75 0.42833476329047215 1101 76-77 0.31817873371313965 1101 78-79 0.47220943511950964 1101 80-81 0.3812986017505864 1101 82-83 0.4326571536489041 1101 84-85 0.341026319879127 1101 86-87 0.19914366119464688 1101 88-89 0.3341040031010962 1101 90-91 0.4135321749162273 1101 92-93 0.14474399196566168 1101 94-95 0.3270278276400447 1101 96-97 0.1979314632993905 1101 98-99 -0.06067063994979094 1101 100-101 0.10891980419802394 1102 1 0.13147863007922922 1102 2 0.20741345830817437 1102 3 0.17058321206815918 1102 4 0.15918787798746337 1102 5 -0.05275304714283635 1102 6 0.07564383286283771 1102 7 0.18811117984765247 1102 8 0.06985024290521125 1102 9 0.1256801766939546 1102 10-11 0.18750270676791558 1102 12-13 0.15240793805757136 1102 14-15 0.24617314962336678 1102 16-17 0.2978430571591275 1102 18-19 0.3017158344696682 1102 20-21 0.23647782079449797 1102 22-23 0.1206181983822674 1102 24-25 0.2110738084188455 1102 26-27 0.1526826261801162 1102 28-29 -0.1658164041361232 1102 30-31 -0.1084508379330984 1102 32-33 0.21163772352938537 1102 34-35 0.13671756933389645 1102 36-37 0.2887613215523146 1102 38-39 0.2923369677436156 1102 40-41 0.19333891596813402 1102 42-43 0.3002498455853626 1102 44-45 0.2947777942917824 1102 46-47 0.31316711682595866 1102 48-49 0.39560495481788394 1102 50-51 0.44796802313599926 1102 52-53 0.27221147066445184 1102 54-55 0.21056208260787201 1102 56-57 0.15463973715932156 1102 58-59 0.07413870008504375 1102 60-61 0.39847045387640634 1102 62-63 0.2657546494598151 1102 64-65 0.3531010205121632 1102 66-67 0.47438801943155084 1102 68-69 0.3310431107059131 1102 70-71 0.4068192685591754 1102 72-73 0.5348014293535144 1102 74-75 0.5314981401309566 1102 76-77 0.4816797017293126 1102 78-79 0.3880331061511839 1102 80-81 0.34591153816157316 1102 82-83 0.09029197937027078 1102 84-85 0.2609676316660554 1102 86-87 0.31438312910723454 1102 88-89 0.17379105883168222 1102 90-91 0.3414435460700531 1102 92-93 0.39101212887386794 1102 94-95 0.29731580352179066 1102 96-97 0.221111164250253 1102 98-99 0.20913975747264502 1102 100-101 0.13850144975408085 1103 1 0.19288750072107774 1103 2 0.19345481298103806 1103 3 0.1781644493124901 1103 4 0.1613170765326828 1103 5 0.05680333020864481 1103 6 0.1443237863623139 1103 7 0.2678870763240013 1103 8 0.10466733671333373 1103 9 0.20675159030746926 1103 10-11 0.23510093458649806 1103 12-13 0.12468817273350652 1103 14-15 0.2081905567553335 1103 16-17 0.30570190521856944 1103 18-19 0.283791967184051 1103 20-21 0.18517443660264377 1103 22-23 0.1674158543395592 1103 24-25 0.23500210615799944 1103 26-27 0.20081343662257467 1103 28-29 0.2862984186400723 1103 30-31 0.3032120423597604 1103 32-33 0.22910824471326663 1103 34-35 0.3002801452453099 1103 36-37 0.36391914372518386 1103 38-39 0.48778092431746956 1103 40-41 0.46390362334199864 1103 42-43 0.24322360358117123 1103 44-45 0.43123327298791736 1103 46-47 0.4036190027117783 1103 48-49 0.16910169649889184 1103 50-51 0.3328447141282851 1103 52-53 0.3571394390060689 1103 54-55 0.30051958919298727 1103 56-57 0.32751203844826904 1103 58-59 0.39771801754164215 1103 60-61 0.38017049609815246 1103 62-63 0.26041240994139514 1103 64-65 0.40334008841900015 1103 66-67 0.31164271164860224 1103 68-69 0.3290508781489834 1103 70-71 0.4284112208452058 1103 72-73 0.46249442405260055 1103 74-75 0.4181276561882399 1103 76-77 0.34977438031768315 1103 78-79 0.4008818741930611 1103 80-81 0.4559736143169246 1103 82-83 0.34175332722879403 1103 84-85 0.22579736935921346 1103 86-87 0.21388999495187733 1103 88-89 0.3025407857486684 1103 90-91 0.3497571551691294 1103 92-93 0.2871967205397752 1103 94-95 0.3205558754685569 1103 96-97 0.280598549229957 1103 98-99 0.1502856986949901 1103 100-101 0.254167528938531 1104 1 0.1419505867442581 1104 2 0.10476548554616727 1104 3 0.15372053693670296 1104 4 0.0712516910087686 1104 5 0.08951457173014177 1104 6 0.11682378234502266 1104 7 0.08700533858063864 1104 8 0.12220176628663637 1104 9 0.18437203288502957 1104 10-11 0.08379333123617982 1104 12-13 0.1474156280651826 1104 14-15 0.21558988195658202 1104 16-17 0.24569018542449328 1104 18-19 0.1687191764428988 1104 20-21 0.1362859423015763 1104 22-23 0.24665007185314636 1104 24-25 0.2050539614260387 1104 26-27 0.1915654853100719 1104 28-29 0.12211738745888567 1104 30-31 0.11127068632387704 1104 32-33 0.15552957773807208 1104 34-35 0.0956966237685748 1104 36-37 0.16735749455518345 1104 38-39 0.09065144362541133 1104 40-41 0.07332688949006183 1104 42-43 0.14971030606070457 1104 44-45 0.15142399281787533 1104 46-47 0.37558255565198806 1104 48-49 0.2990280406998167 1104 50-51 0.2202554163142878 1104 52-53 0.18425091685174522 1104 54-55 0.17175397596869146 1104 56-57 0.13023986337125137 1104 58-59 0.18487935271971168 1104 60-61 0.2854921824843828 1104 62-63 0.25829261629118605 1104 64-65 0.13714355261443956 1104 66-67 0.19021911019016358 1104 68-69 0.16782497054787626 1104 70-71 0.3144565777338215 1104 72-73 0.24888925898722647 1104 74-75 0.19246296763854787 1104 76-77 0.1602960289948996 1104 78-79 0.2384318962346299 1104 80-81 0.2790134278714973 1104 82-83 0.36802845742938217 1104 84-85 0.30899424850874624 1104 86-87 0.32371686552581735 1104 88-89 0.26441503150117995 1104 90-91 0.1540716784472167 1104 92-93 0.1738577951291873 1104 94-95 0.1993854976230338 1104 96-97 0.11876509243169409 1104 98-99 0.28825721552452066 1104 100-101 0.2820720747509817 1105 1 0.12249706806162663 1105 2 0.1885021847317745 1105 3 0.027241024115141954 1105 4 0.07884043211426928 1105 5 0.08791420983180842 1105 6 0.09753563933585241 1105 7 0.14631711374882883 1105 8 0.1608658077071894 1105 9 0.2127929176819663 1105 10-11 0.15145212834445942 1105 12-13 0.15377740032878506 1105 14-15 0.2050359970496487 1105 16-17 0.1453198823469961 1105 18-19 0.15580998598030504 1105 20-21 0.17315618914145858 1105 22-23 0.06410326176202119 1105 24-25 0.20376469860850221 1105 26-27 0.11880364976369862 1105 28-29 0.08901419575782654 1105 30-31 0.08745448815982115 1105 32-33 0.1773301208327709 1105 34-35 0.20810709162846308 1105 36-37 0.2456659659178726 1105 38-39 0.19120467345680225 1105 40-41 0.11333729653917146 1105 42-43 0.025510456110076518 1105 44-45 0.010681631554838589 1105 46-47 0.09501357928785836 1105 48-49 0.20327948093900972 1105 50-51 0.23541744863051406 1105 52-53 0.2706668749862402 1105 54-55 0.15608592869943294 1105 56-57 0.053967935335627715 1105 58-59 -0.02376035202661342 1105 60-61 0.05357269991990421 1105 62-63 0.09796300205652386 1105 64-65 0.05833425746335763 1105 66-67 0.16292285413226182 1105 68-69 0.13533433627407732 1105 70-71 0.19876887819843603 1105 72-73 0.2756384651716317 1105 74-75 0.28339091303738684 1105 76-77 0.20398054461597326 1105 78-79 0.3300738739694751 1105 80-81 0.29374842509669463 1105 82-83 0.2557576566180799 1105 84-85 0.22286664720996896 1105 86-87 0.15914545178629425 1105 88-89 0.26062066094843317 1105 90-91 0.23690313739932378 1105 92-93 0.140662645654551 1105 94-95 0.12509906646220514 1105 96-97 0.17740473537845247 1105 98-99 0.1804835700785965 1105 100-101 0.26845082953890653 1106 1 0.16664268426270468 1106 2 0.19985357555727745 1106 3 0.16388195630712232 1106 4 0.17861450426968872 1106 5 0.10495624382139823 1106 6 0.010812665668993304 1106 7 0.1772194786884782 1106 8 0.17233746517318593 1106 9 0.1662435661405226 1106 10-11 0.28374315079346957 1106 12-13 0.22141506227227126 1106 14-15 0.20354453096050662 1106 16-17 0.2141444642093262 1106 18-19 0.17611177081028728 1106 20-21 0.16551629074051988 1106 22-23 0.17363981300770348 1106 24-25 0.18585056031783864 1106 26-27 0.18626490542045104 1106 28-29 0.23260914136399435 1106 30-31 0.17126253621170662 1106 32-33 0.1432523286270566 1106 34-35 0.04947288250144055 1106 36-37 0.14719691232733823 1106 38-39 0.147900809634244 1106 40-41 0.006256854816001578 1106 42-43 0.20663840657939403 1106 44-45 0.1830594422455718 1106 46-47 0.1535349385975664 1106 48-49 0.25202056078282453 1106 50-51 0.3565275530822092 1106 52-53 0.19911176114370477 1106 54-55 0.22411074881294013 1106 56-57 0.14246467079238556 1106 58-59 0.19141652209049909 1106 60-61 0.3596304187789414 1106 62-63 0.24834673707739086 1106 64-65 0.3932528892078153 1106 66-67 0.41176925286523414 1106 68-69 0.2701639661403732 1106 70-71 0.3876369112340967 1106 72-73 0.4403966881464427 1106 74-75 0.45000254307255005 1106 76-77 0.3182970672441314 1106 78-79 0.4146884159635249 1106 80-81 0.3195266780582955 1106 82-83 0.2724538384375066 1106 84-85 0.2423780268988267 1106 86-87 0.22282675203526736 1106 88-89 0.3631841432496117 1106 90-91 0.3971480523112447 1106 92-93 0.3617242554931366 1106 94-95 0.39344440744209663 1106 96-97 0.28063983954487526 1106 98-99 0.3841458832602278 1106 100-101 0.31945518150253704 1107 1 0.103551567721901 1107 2 0.06152501154537049 1107 3 0.055724265888109414 1107 4 0.055403532175645864 1107 5 0.09470940748074241 1107 6 0.13619975157597963 1107 7 0.20086776043378052 1107 8 0.037478034161253504 1107 9 0.012254606207505958 1107 10-11 0.10350411260212411 1107 12-13 0.01785025181499833 1107 14-15 0.04886231938662178 1107 16-17 0.15345976455306243 1107 18-19 -0.027279593644387035 1107 20-21 -0.005706004215213056 1107 22-23 0.047800958292206985 1107 24-25 0.09258718602328742 1107 26-27 0.039341873707833486 1107 28-29 0.02786321580117601 1107 30-31 -0.006992041208242483 1107 32-33 0.0919626740156545 1107 34-35 0.0427522491775747 1107 36-37 0.1459299792487414 1107 38-39 0.14876931733422083 1107 40-41 0.10086510109271529 1107 42-43 -0.01729624739955682 1107 44-45 0.021881683197733537 1107 46-47 0.19882585300829447 1107 48-49 0.1347880988461796 1107 50-51 0.02737133328086827 1107 52-53 0.05933738788109366 1107 54-55 0.1472672488536162 1107 56-57 0.11822088045740742 1107 58-59 0.04404938769103239 1107 60-61 0.2525901720661139 1107 62-63 0.15549318087003172 1107 64-65 -0.021435953561308452 1107 66-67 0.1427843155166144 1107 68-69 0.18493798803907424 1107 70-71 0.14193675369530467 1107 72-73 0.1498570655836815 1107 74-75 0.11641413373742893 1107 76-77 -0.046807939457337966 1107 78-79 -0.04162363437052363 1107 80-81 0.17148694795687902 1107 82-83 0.15397105335254224 1107 84-85 0.17601166635175503 1107 86-87 0.050893530769279494 1107 88-89 -0.02228357322143637 1107 90-91 0.14360577173374622 1107 92-93 0.10822850401516604 1107 94-95 0.15757434735247244 1107 96-97 0.05504248895652353 1107 98-99 0.1300865653661738 1107 100-101 0.23657556899943089 1108 1 0.033914464224785945 1108 2 0.059638957563457495 1108 3 0.05787366830710994 1108 4 0.03601433166105039 1108 5 0.0666687885863837 1108 6 0.09042072035652637 1108 7 0.11557271690273296 1108 8 0.0966825333544179 1108 9 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-1.6976265953515899 2316 84-85 -1.609957475130713 2316 86-87 -1.1273776112231175 2316 88-89 -1.004192428446359 2316 90-91 -0.9127436413454859 2316 92-93 -0.8696249187849645 2316 94-95 -0.9688774416089423 2316 96-97 -0.9179293156529909 2316 98-99 -0.8502849900155702 2316 100-101 -0.9639437083696691 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 1.0 11 3.0 12 13.0 13 116.0 14 469.0 15 1586.0 16 3539.0 17 5947.0 18 7798.0 19 9644.0 20 11045.0 21 11911.0 22 10621.0 23 10200.0 24 11626.0 25 14338.0 26 18584.0 27 24211.0 28 33176.0 29 33856.0 30 29076.0 31 34034.0 32 42523.0 33 54952.0 34 75301.0 35 110053.0 36 182968.0 37 417346.0 38 921064.0 39 301071.0 40 59.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.41765219835996 29.218987527167005 15.03730570645998 20.32605456801305 2 12.74743941875321 23.86717916854045 45.19016262272848 18.19521878997786 3 14.136542366549817 30.577845572147506 37.54521747507386 17.740394586228817 4 8.340411997167694 22.55171842190183 46.978599085095844 22.129270495834632 5 8.120289491706814 43.057768773463145 40.22666810257066 8.595273632259378 6 20.48779790100003 47.46261461290328 21.66791747366154 10.381670012435153 7 21.245601842893034 37.81283496246738 26.391677029294126 14.549886165345466 8 21.402808089748486 38.16935702350563 25.88442711637385 14.543407770372028 9 24.185783540838287 16.544727007166617 25.846650501528735 33.42283895046636 10-11 20.146861848647866 28.21641793556269 35.979533319983915 15.657186895805534 12-13 20.540823984532622 29.749589000526683 31.933249815323677 17.776337199617018 14-15 19.36293398936197 30.590181782080254 29.538178780143465 20.508705448414307 16-17 17.90903912782294 33.29440687349293 30.73011932025651 18.06643467842762 18-19 17.853320724301387 31.43655884486011 32.54205067282759 18.168069758010912 20-21 18.16401024427756 31.532683081966002 31.305118941649013 18.99818773210743 22-23 18.049438090694693 31.618914682504624 31.572428960983125 18.759218265817555 24-25 18.994969568370625 33.4841943989648 30.340847710602525 17.179988322062048 26-27 20.051431725289127 31.461336602258523 32.29492514509081 16.192306527361545 28-29 18.73401098697653 29.43743499203915 33.836054551146326 17.992499469837988 30-31 18.460047073237845 31.99656922433503 32.052970435751426 17.4904132666757 32-33 18.37178549020111 31.314653817005915 32.677493861824644 17.636066830968332 34-35 20.301333708014532 31.326026489063292 30.64108345687156 17.73155634605062 36-37 18.89144145129509 30.012805346947495 31.740769969862843 19.35498323189457 38-39 17.23116343560223 31.08687275254382 32.49342064302698 19.188543168826975 40-41 17.08600952744736 30.330393936895383 33.469155267776465 19.114441267880792 42-43 17.94147317018996 29.491546956584656 32.62364058874307 19.94333928448231 44-45 18.56811064762075 29.968613438378682 32.831433004141125 18.631842909859444 46-47 18.312003708670783 29.35556376339219 32.040437804884206 20.291994723052824 48-49 17.388860798593424 30.063496692604424 31.63091413817185 20.916728370630302 50-51 15.780374885561956 29.162894138079594 31.58839160697938 23.468339369379073 52-53 16.564561833335297 30.72898349776117 32.52030177541676 20.18615289348677 54-55 17.264209104200624 28.7810226823605 31.966990189564097 21.987778023874775 56-57 18.115443315726683 29.33040739849533 30.858677599729422 21.695471686048567 58-59 17.70322388491678 29.719195231901303 31.373752067617616 21.2038288155643 60-61 19.17718494387354 31.290584620458606 29.717891139406643 19.81433929626121 62-63 17.248600414280737 31.787023185923204 29.43658576806 21.527790631736057 64-65 16.42944102388929 31.55722946811536 28.847872141723723 23.165457366271625 66-67 17.9811217887774 31.31738161784873 28.93034547513558 21.77115111823828 68-69 17.731934953549068 30.854449815996755 28.93730764635704 22.47630758409714 70-71 18.196170847895697 30.7884879762672 28.77404367952642 22.24129749631068 72-73 19.354962198144655 30.060236453003032 28.798590065675782 21.786211283176534 74-75 17.981689700025072 30.749428302677345 28.68254686740156 22.586335129896025 76-77 18.190554836668728 32.44005801949576 28.181586045705497 21.187801098130016 78-79 19.094322112988987 29.735346324794378 28.478555297143547 22.691776265073088 80-81 19.227671256679233 30.331127555284514 28.37577757410133 22.065423613934918 82-83 19.038174413160057 31.005199386961184 28.386427324063707 21.57019887581505 84-85 19.220325164946665 29.74908419052876 28.4123893835092 22.618201261015376 86-87 18.243749190200628 30.264137624667036 29.457072640476 22.035040544656333 88-89 19.765393760211886 29.29231527740151 28.15180225582761 22.790488706558996 90-91 19.14496123900566 28.842171995497097 29.716923586910614 22.295943178586633 92-93 18.77666027801569 30.01034439820759 29.505534400277313 21.70746092349941 94-95 18.182225471702875 28.80826559063611 29.963418102149987 23.046090835511027 96-97 20.000635219247396 27.185827291038105 29.42064218562537 23.39289530408913 98-99 18.306240461194413 29.14930681173784 28.516842985580944 24.0276097414868 100-101 17.731240839801913 29.443842411780246 30.208966098643238 22.615950649774604 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 162.0 1 105.5 2 120.5 3 247.0 4 191.0 5 111.5 6 184.5 7 329.5 8 452.0 9 474.5 10 387.0 11 256.0 12 237.0 13 250.0 14 253.5 15 275.0 16 280.5 17 308.0 18 348.0 19 389.5 20 448.0 21 529.5 22 632.0 23 762.0 24 938.0 25 1195.0 26 1698.0 27 3634.0 28 6441.5 29 8582.5 30 13548.5 31 23914.5 32 47637.5 33 83096.5 34 137263.5 35 184205.5 36 205179.0 37 217921.5 38 207026.5 39 192464.0 40 177761.5 41 157658.0 42 140485.0 43 126776.0 44 112544.0 45 87106.5 46 60488.5 47 40176.0 48 32464.5 49 29608.0 50 25234.5 51 18505.5 52 9079.0 53 3985.0 54 2653.0 55 2128.0 56 1606.5 57 1302.0 58 1193.0 59 1031.5 60 805.0 61 590.0 62 453.5 63 331.5 64 224.0 65 147.0 66 101.0 67 74.0 68 59.0 69 44.0 70 24.0 71 15.5 72 16.5 73 18.0 74 13.0 75 9.5 76 6.5 77 4.0 78 3.0 79 2.0 80 1.5 81 0.5 82 1.5 83 2.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0457694398123453 2 0.001472362493963314 3 3.7860749844770926E-4 4 0.0114002924532588 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0024399149899963484 24-25 0.0 26-27 0.0 28-29 0.021033749913761626 30-31 0.017247674929284532 32-33 2.1033749913761626E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.03645148860054889 50-51 0.012620249948256976 52-53 0.0 54-55 8.623837464642266E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.734387488789011E-4 80-81 0.011295123703689992 82-83 0.005090167479130313 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2377132.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 10.548780682645152 #Duplication Level Percentage of deduplicated Percentage of total 1 68.2700049757657 7.201653096924457 2 12.500951758396345 2.637395968473006 3 5.228662285019647 1.6546803512487152 4 2.7960425582090562 1.179793589035577 5 1.7119834691960223 0.9029669074431417 6 1.192108231117416 0.7545172968020208 7 0.8735889857070103 0.6450709031898377 8 0.6534530309438662 0.5514506167869263 9 0.5292784114753474 0.5024917694441031 >10 4.197074832198089 9.326939304106933 >50 0.7812156684695637 5.839985110970303 >100 0.9597080861188483 21.911569771681314 >500 0.17766222355487332 13.336070329780261 >1k 0.11913331345852485 24.496541504267718 >5k 0.007056677103815061 4.9626080118103975 >10k+ 0.002075493265827959 4.09626546803525 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 33179 1.395757576777394 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 23762 0.9996079309016075 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 12944 0.5445217177674609 No Hit ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 12074 0.5079229929175157 TruSeq Adapter, Index 3 (95% over 21bp) GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 11589 0.48752025550116695 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 9439 0.3970751308719919 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 8647 0.3637576710085935 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 8502 0.35765788353360267 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 8115 0.3413777611003512 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 7019 0.2952717812893857 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 6762 0.2844604338337122 No Hit ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGAC 6750 0.283955623835782 No Hit AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT 6351 0.26717069140460015 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 6318 0.2657824639102919 No Hit GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC 6294 0.26477284391443134 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 6139 0.25825238144116525 No Hit TTTGTAAGGTGGCTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGT 5741 0.241509516509811 No Hit AACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGACCCAGA 5675 0.23873306152119445 No Hit CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC 5621 0.23646141653050817 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 5537 0.23292774654499623 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 5326 0.22405150408138885 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 5097 0.214418046620886 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 4972 0.20915960914244558 No Hit CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG 4953 0.20836032664572268 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 4830 0.2031860241669373 No Hit GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCT 4717 0.19843239668642718 No Hit GATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGA 4706 0.1979696541883244 No Hit CTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGAATAAAAA 4688 0.197212439191429 No Hit GTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG 4650 0.19561387419798315 No Hit TTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGT 4589 0.19304775670850421 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 4358 0.18333016424834633 No Hit ATACGATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTA 4272 0.17971235926317933 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 4132 0.17382290928732608 No Hit GTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGAATAAAAAATCCT 4061 0.17083611679957192 No Hit GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT 4059 0.17075198179991688 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 4047 0.1702471718019866 No Hit GTACAAGGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCT 4011 0.16873274180819578 No Hit GTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATATATCTGGGTCAAT 3976 0.16726037931423246 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 3918 0.1648204643242361 No Hit TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG 3878 0.16313776433113517 TruSeq Adapter, Index 3 (96% over 28bp) GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC 3868 0.16271708933285994 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 3827 0.16099232183993148 No Hit GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG 3642 0.1532098343718397 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 3637 0.15299949687270206 No Hit CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA 3599 0.15140093187925618 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 3593 0.15114852688029104 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 3567 0.15005477188477545 No Hit CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCA 3512 0.14774105939426166 No Hit CTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGTAAATAGATAGAAAC 3462 0.1456376844028855 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 3444 0.1448804694059901 No Hit CCCTAATTGTCGATATGAACTCTTAAATAGGATTGCGCTGTTATCCCTAG 3427 0.14416532190892217 No Hit ACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTC 3390 0.14260882441530381 No Hit GGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTA 3316 0.1394958294280671 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 3286 0.1382338044332414 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 3255 0.1369297119385882 No Hit ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT 3215 0.13524701194548724 RNA PCR Primer, Index 3 (95% over 23bp) ATCTTATTGACCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGG 3208 0.1349525394466946 No Hit GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT 3196 0.1344477294487643 No Hit GATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGT 3127 0.1315450719606652 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 3106 0.1306616544642872 No Hit AGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGTTCTTA 2980 0.12536114948601929 No Hit TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGT 2976 0.12519287948670918 No Hit AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCGACAATTAGGGTT 2964 0.12468806948877892 No Hit GTTTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTT 2945 0.12388878699205598 No Hit CTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATA 2916 0.1226688294970578 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 2898 0.12191161450016239 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 2895 0.12178541200067981 No Hit GCCATGAAGTCATTCTAAATTTGTTGAAGCATACGATACTGATATTACTT 2866 0.12056545450568164 No Hit ATTAAGTTGAGAGCGCTTATTTGTAAGGTGGCTCTATTTCTCTTGTCCTT 2863 0.12043925200619908 No Hit TTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGACC 2849 0.11985030700861375 No Hit AGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAAT 2849 0.11985030700861375 No Hit GGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGG 2838 0.11938756451051098 No Hit ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCTCGATGTTGGATC 2826 0.11888275451258071 No Hit GGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAG 2796 0.117620729517755 No Hit GATGAAAGACTAATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACC 2786 0.11720005451947978 No Hit TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC 2781 0.11698971702034217 No Hit CTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCT 2778 0.1168635145208596 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 2764 0.11627456952327427 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 2737 0.11513874702793114 No Hit CTCTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGAT 2723 0.11454980203034582 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 2708 0.11391878953293297 No Hit CTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAA 2695 0.11337191203517516 No Hit ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT 2602 0.10945963455121549 No Hit GATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAGATCAT 2581 0.1085762170548375 No Hit GTCGTAAACCCTAATTGTCGATATGAACTCTTAAATAGGATTGCGCTGTT 2574 0.10828174455604483 No Hit AACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAACAAG 2568 0.1080293395570797 No Hit TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA 2563 0.10781900205794209 No Hit ACGTAGGACTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACC 2553 0.10739832705966687 No Hit CGTATAACAACTCGGATAACCATTGTTAGTTAATCAGACTATAGGCAATA 2508 0.10550528956742831 No Hit ATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATA 2474 0.10407499457329253 No Hit GATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTAT 2438 0.10256056457950168 No Hit ACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGT 2438 0.10256056457950168 No Hit GCTATACTATGAGCCTTAGGCTTTATTTTCTTATTTACAGTTGGTGGTCT 2436 0.10247642957984662 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 2415 0.10159301208346865 No Hit AAACTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGAATAA 2413 0.1015088770838136 No Hit ACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGACCCAGAT 2407 0.10125647208484848 No Hit GTACATGGGAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGAC 2395 0.10075166208691817 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.2620249948256976E-4 0.0 2 0.0 0.0 0.0 2.9447249879266277E-4 0.0 3 0.0 0.0 0.0 5.04809997930279E-4 0.0 4 0.0 0.0 0.0 7.992824967229418E-4 0.0 5 0.0 0.0 0.0 8.834174963779882E-4 0.0 6 0.0 0.0 0.0 9.254849962055115E-4 0.0 7 0.0 0.0 0.0 0.001346159994480744 0.0 8 0.0 0.0 0.0 0.0016406324932734069 0.0 9 0.0 0.0 0.0 0.0029026574880991044 0.0 10-11 0.0 0.0 0.0 0.005910483725767017 0.0 12-13 0.0 0.0 0.0 0.012052338700585412 0.0 14-15 0.0 0.0 0.0 0.025577039895134136 0.0 16-17 0.0 0.0 0.0 0.06381639723835278 0.0 18-19 0.0 0.0 0.0 0.10405396082337875 0.0 20-21 0.0 0.0 0.0 0.16467322807483975 0.0 22-23 0.0 0.0 0.0 0.2783396126088076 0.0 24-25 0.0 0.0 0.0 0.4321173582283188 0.0 26-27 0.0 0.0 0.0 0.5570367989661491 0.0 28-29 0.0 0.0 0.0 0.6898018284218125 0.0 30-31 0.0 0.0 0.0 0.8924620088409058 0.0 32-33 0.0 0.0 0.0 1.2339239049409119 0.0 34-35 0.0 0.0 0.0 1.767150499004683 0.0 36-37 0.0 0.0 0.0 2.3984574689163245 0.0 38-39 0.0 0.0 0.0 3.3268661563598485 0.0 40-41 0.0 0.0 0.0 4.331185647242139 0.0 42-43 0.0 0.0 0.0 5.147926156393503 0.0 44-45 0.0 0.0 0.0 6.07160225010643 0.0 46-47 0.0 0.0 0.0 7.036925168648606 0.0 48-49 0.0 0.0 0.0 8.034471792058666 0.0 50-51 0.0 0.0 0.0 9.164215533676717 0.0 52-53 0.0 0.0 0.0 10.102299746080572 0.0 54-55 0.0 0.0 0.0 10.87958094039372 0.0 56-57 0.0 0.0 0.0 11.762977403021793 0.0 58-59 0.0 0.0 0.0 12.762332928924437 0.0 60-61 0.0 0.0 0.0 13.736490022430392 0.0 62-63 0.0 0.0 0.0 14.637786206235077 0.0 64-65 0.0 0.0 0.0 15.527703131336418 0.0 66-67 0.0 0.0 0.0 16.38754179406108 0.0 68-69 0.0 0.0 0.0 17.304339851552207 0.0 70-71 0.0 0.0 0.0 18.340441338554193 0.0 72-73 0.0 0.0 0.0 19.298823119624824 0.0 74-75 0.0 0.0 0.0 20.137628032435725 0.0 76-77 0.0 0.0 0.0 21.066583597377008 0.0 78-79 0.0 0.0 0.0 21.997095659811908 0.0 80-81 0.0 0.0 0.0 23.108708309004296 0.0 82-83 0.0 0.0 0.0 24.1193168911108 0.0 84-85 0.0 0.0 0.0 25.05843175726043 0.0 86-87 0.0 0.0 0.0 26.032651952016128 0.0 88-89 0.0 0.0 0.0 27.070330970261644 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTATAT 855 0.0 61.68647 1 GACATAT 730 0.0 59.23135 1 AACTTGC 400 0.0 54.618412 7 TAACTTT 5100 0.0 52.616196 6 ACATATA 875 0.0 49.944187 2 GTGCGTA 40 5.3925556E-4 47.49427 5 AACTTTA 5940 0.0 45.415394 7 ATACTCA 190 0.0 44.994568 6 CATATAG 970 0.0 44.561165 3 TGGGCGA 140 0.0 44.101818 6 GGCGATA 130 0.0 43.840862 8 ACTTTAT 6255 0.0 42.900497 8 GGTAAGG 100 6.348273E-10 42.763733 1 GGGCGAT 135 0.0 42.21713 7 GCGATAT 135 0.0 42.21713 9 AAACTTG 545 0.0 41.829815 6 CGCAGAA 330 0.0 41.737385 9 TATGGGC 370 0.0 39.79668 4 ACTTGCT 580 0.0 39.305603 8 ATGGGCG 135 7.2759576E-12 38.699036 5 >>END_MODULE