FastQCFastQC Report
Fri 10 Feb 2017
SRR2031644_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031644_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2442858
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39400.025.988261
GGTATCA28100.025.5920771
TCAACGC39850.024.6884614
GTACATG56550.024.6757281
CAACGCA40550.024.3794845
TACATGG56350.024.3212262
ATCAACG41900.023.7059573
AACGCAG43100.023.371466
ACATGGG56750.022.7786673
TATCAAC46650.021.3197232
ACGCAGA48000.020.787227
CGCAGAG49150.020.3012648
CATGGGG39600.019.6834244
GAGTACA49050.019.4189591
AGTACAT45900.018.1431312
GTGTAGC14050.017.96531
TATACAG18700.017.791335
CTATACT13000.017.5488994
TATACGA2202.8739292E-517.3043082
TATACTG16000.017.2289965
Produced by FastQC (version 0.11.5)