FastQCFastQC Report
Fri 10 Feb 2017
SRR2031644_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031644_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2442858
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC1252.1554843E-730.4001983
GTACATG53900.025.3919331
TACATGG54400.023.8374712
GGTATCA34150.023.6564881
ACATGGG52500.023.5239623
GTATCAA45550.022.3263361
TCAACGC44700.021.8900744
CAACGCA45900.021.5229915
TATACTG15050.021.1441525
ATCAACG47000.020.8192843
TAGTGCG1150.003726530820.6501929
AACGCAG48500.020.2712546
GTACCGT2651.859371E-819.715096
CATGGGG35300.019.6456184
TATCAAC51400.019.4991552
ACGCAGA53500.018.465527
GTATAGG7050.018.1998021
CTATACT12450.017.9314774
TATACAG20050.017.7663765
CGCAGAG55400.017.7464948
Produced by FastQC (version 0.11.5)