##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031643_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1792887 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.36285164653433 34.0 31.0 34.0 31.0 34.0 2 32.58447186018974 34.0 31.0 34.0 31.0 34.0 3 32.70539470697261 34.0 31.0 34.0 31.0 34.0 4 35.837138648448004 37.0 35.0 37.0 35.0 37.0 5 35.6645745102731 37.0 35.0 37.0 35.0 37.0 6 35.86480352637952 37.0 37.0 37.0 35.0 37.0 7 35.962565404289286 37.0 37.0 37.0 35.0 37.0 8 36.02079216370022 37.0 37.0 37.0 35.0 37.0 9 37.941420178739655 39.0 39.0 39.0 35.0 39.0 10-11 37.913883306644536 39.0 39.0 39.0 36.0 39.0 12-13 37.98856313866964 39.0 39.0 39.0 37.0 39.0 14-15 39.46103379632961 41.0 40.0 41.0 37.0 41.0 16-17 39.43698013315954 41.0 39.5 41.0 37.0 41.0 18-19 39.4396406466219 41.0 40.0 41.0 37.0 41.0 20-21 39.48149325640713 41.0 40.0 41.0 37.0 41.0 22-23 39.405515796589526 41.0 39.5 41.0 37.0 41.0 24-25 39.34734788082015 41.0 39.0 41.0 36.0 41.0 26-27 39.27080764153011 41.0 39.0 41.0 36.0 41.0 28-29 39.184146017010555 41.0 39.0 41.0 36.0 41.0 30-31 39.04271267514349 41.0 39.0 41.0 35.5 41.0 32-33 38.90328475804666 40.5 39.0 41.0 35.0 41.0 34-35 38.844550716247035 40.0 38.5 41.0 35.0 41.0 36-37 38.710985968440845 40.0 38.0 41.0 35.0 41.0 38-39 38.50459259284048 40.0 38.0 41.0 35.0 41.0 40-41 38.454811987593196 40.0 38.0 41.0 35.0 41.0 42-43 38.34448964156692 40.0 38.0 41.0 34.5 41.0 44-45 38.13008181776097 40.0 38.0 41.0 34.0 41.0 46-47 37.91284308492392 40.0 38.0 41.0 33.0 41.0 48-49 37.99641667322034 40.0 38.0 41.0 33.5 41.0 50-51 37.77721211654722 40.0 38.0 40.5 33.5 41.0 52-53 37.94787652540288 40.0 38.5 41.0 33.5 41.0 54-55 38.25072020712962 41.0 39.0 41.0 34.0 41.0 56-57 38.19279184912379 41.0 39.0 41.0 34.0 41.0 58-59 38.07819065005212 41.0 38.5 41.0 34.0 41.0 60-61 37.88463243918886 40.0 38.0 41.0 33.5 41.0 62-63 37.59789434582325 40.0 38.0 41.0 33.0 41.0 64-65 37.40256329595786 40.0 37.0 41.0 32.5 41.0 66-67 37.21482558577311 39.5 37.0 41.0 32.0 41.0 68-69 36.935688640723036 39.0 36.5 41.0 32.0 41.0 70-71 36.60955012781062 39.0 36.0 41.0 32.0 41.0 72-73 36.239708637521495 38.0 35.5 40.0 31.5 41.0 74-75 35.72597687416999 37.0 35.0 39.5 31.0 41.0 76-77 35.267194474609944 37.0 35.0 39.0 30.5 41.0 78-79 34.844796130486756 36.0 35.0 39.0 30.0 40.5 80-81 34.40408570088354 36.0 35.0 37.0 29.5 39.0 82-83 33.9921590150411 35.0 35.0 37.0 29.0 39.0 84-85 33.61286768212386 35.0 34.0 36.5 29.0 38.0 86-87 33.32092848015519 35.0 34.0 36.0 29.0 37.0 88-89 33.027625276997384 35.0 34.0 36.0 28.5 37.0 90-91 32.82630444640404 35.0 34.0 36.0 27.0 36.5 92-93 32.63482333242419 35.0 34.0 35.0 27.0 36.0 94-95 32.42915755426862 35.0 34.0 35.0 26.0 36.0 96-97 32.2962342300435 35.0 34.0 35.0 25.5 36.0 98-99 32.20178544436989 35.0 34.0 35.0 25.0 36.0 100-101 31.800316472817304 35.0 33.0 35.0 24.0 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20592910200144132 1101 2 0.22548965290302192 1101 3 0.2352623884469267 1101 4 0.2515810035096919 1101 5 0.2827270816203722 1101 6 0.35275514401902086 1101 7 0.28644733131050515 1101 8 0.28262924168463144 1101 9 0.2536801977120646 1101 10-11 0.2856218945649118 1101 12-13 0.18752616937580058 1101 14-15 0.3023327700658385 1101 16-17 0.23095831161646174 1101 18-19 0.35203224722983606 1101 20-21 0.06520068251973754 1101 22-23 0.19655204664044135 1101 24-25 0.29646795821879834 1101 26-27 0.26079029026772815 1101 28-29 0.286369040950909 1101 30-31 0.33404072559306996 1101 32-33 0.28368481591223826 1101 34-35 0.3049086037869202 1101 36-37 0.3020062564136268 1101 38-39 0.30332948461503406 1101 40-41 0.2771809593213419 1101 42-43 0.2554835113800351 1101 44-45 0.34875896158114017 1101 46-47 0.5213252381804381 1101 48-49 0.4826552606195236 1101 50-51 0.41413689775415463 1101 52-53 0.37223183148516625 1101 54-55 0.46079917753105804 1101 56-57 0.45468378143645083 1101 58-59 0.513474316116266 1101 60-61 0.4342572416839019 1101 62-63 0.36932078463423323 1101 64-65 0.39013653067510035 1101 66-67 0.3248645654050719 1101 68-69 0.220262038488805 1101 70-71 0.2792404532804298 1101 72-73 0.36831251839854673 1101 74-75 0.3045406705309688 1101 76-77 0.25305493367331877 1101 78-79 0.3206633763800326 1101 80-81 0.3900628979332339 1101 82-83 0.4363758365397814 1101 84-85 0.41229377651981025 1101 86-87 0.49375688065281054 1101 88-89 0.46753208399412927 1101 90-91 0.424553749456976 1101 92-93 0.3107752515460689 1101 94-95 0.2860443284286376 1101 96-97 0.42439671318226146 1101 98-99 0.32163891772606235 1101 100-101 0.3952018893941407 1102 1 -0.014804288467011872 1102 2 0.04279883602615797 1102 3 0.10932843372952306 1102 4 0.10206454355358119 1102 5 0.04072924844287229 1102 6 0.20250816620374934 1102 7 0.17983405205578862 1102 8 0.2767113811888109 1102 9 0.293645136659606 1102 10-11 0.3145318059230391 1102 12-13 0.32527230300539856 1102 14-15 0.24930815979426058 1102 16-17 0.25778391664789524 1102 18-19 0.38089317203881734 1102 20-21 0.2820531968613764 1102 22-23 0.23003839556354677 1102 24-25 0.2732775831720744 1102 26-27 0.2429465830417854 1102 28-29 0.2371033160308329 1102 30-31 0.3137650502207521 1102 32-33 0.28074895214621165 1102 34-35 0.1414573729879791 1102 36-37 0.1491042551941959 1102 38-39 0.21100886337047342 1102 40-41 0.29950669799570306 1102 42-43 0.18436669415503104 1102 44-45 -0.18630399326847424 1102 46-47 -0.6408906279445574 1102 48-49 -0.025668647955399138 1102 50-51 -0.02623315519888081 1102 52-53 0.06058011919125761 1102 54-55 0.045706183939259404 1102 56-57 0.06553463499140122 1102 58-59 0.12192007278724049 1102 60-61 -0.08248355478982461 1102 62-63 -0.32737947190309313 1102 64-65 0.0661028735866509 1102 66-67 0.10432022496853222 1102 68-69 0.32119906081049976 1102 70-71 0.22151979265638744 1102 72-73 0.23003490449055164 1102 74-75 0.21005441577618456 1102 76-77 0.2371098119250945 1102 78-79 0.3170749927554297 1102 80-81 0.165282647787933 1102 82-83 0.18145530413684696 1102 84-85 0.28129542113618555 1102 86-87 0.2043084406603839 1102 88-89 0.06472044631196638 1102 90-91 0.1147145394816107 1102 92-93 0.006335025123583193 1102 94-95 -0.0044808644499525485 1102 96-97 -0.0536722941219665 1102 98-99 -0.13023916949663317 1102 100-101 -0.076406362794323 1103 1 0.15454479690426837 1103 2 0.12401105266204837 1103 3 0.11533730070006243 1103 4 0.21597149017740946 1103 5 0.271634305676109 1103 6 0.20061254880712198 1103 7 0.17938305345415273 1103 8 0.21255616294818225 1103 9 0.28856940091809946 1103 10-11 0.323428353782262 1103 12-13 0.2549701161514335 1103 14-15 0.31843170138754573 1103 16-17 0.30098882657271986 1103 18-19 0.34453774777988855 1103 20-21 0.0690969054753694 1103 22-23 0.18925726954368116 1103 24-25 0.22177834640048388 1103 26-27 0.2268898764168199 1103 28-29 0.14129858152401198 1103 30-31 0.30694069416135505 1103 32-33 0.2426991697285743 1103 34-35 0.21648726165270205 1103 36-37 0.2389463789973192 1103 38-39 0.20824527381299873 1103 40-41 0.2093441161132077 1103 42-43 0.1600817092950635 1103 44-45 0.11098458890577945 1103 46-47 0.30819856837059945 1103 48-49 0.14953474618712903 1103 50-51 0.07478897486662106 1103 52-53 0.013677821322247041 1103 54-55 -0.013836083614101824 1103 56-57 0.12917236124680898 1103 58-59 0.0684307046122612 1103 60-61 0.04123639197987927 1103 62-63 0.1407172695208203 1103 64-65 0.09660688009099516 1103 66-67 -0.05926372837668481 1103 68-69 0.05635546926045265 1103 70-71 0.14516781982660376 1103 72-73 0.017066381880127324 1103 74-75 0.06421574518129347 1103 76-77 -0.2672086998328922 1103 78-79 -0.06066880445708733 1103 80-81 0.0736732232514754 1103 82-83 -0.06322144461690726 1103 84-85 -0.05833721515079304 1103 86-87 -0.024391660391771097 1103 88-89 -0.017465820558228984 1103 90-91 0.09696325231202252 1103 92-93 0.045949959493064796 1103 94-95 -0.056440217645011614 1103 96-97 -0.05378771240315672 1103 98-99 0.029674304598081847 1103 100-101 0.11609153788279869 1104 1 0.18363754511466368 1104 2 0.17294507071371612 1104 3 0.09823490418590097 1104 4 0.052463864905078594 1104 5 0.12652070468015353 1104 6 0.09125991886625684 1104 7 0.09987791806666024 1104 8 0.2337279650023376 1104 9 0.18483457365386613 1104 10-11 0.2640329289456673 1104 12-13 0.2267293754106916 1104 14-15 0.2121875370551507 1104 16-17 0.19781033295304695 1104 18-19 0.2659079625324594 1104 20-21 0.07984231475018788 1104 22-23 0.05281158015681342 1104 24-25 0.0947475340755517 1104 26-27 0.1839938291090064 1104 28-29 0.2390421233852038 1104 30-31 0.24325021797088198 1104 32-33 0.19283144486085746 1104 34-35 0.15880752309060853 1104 36-37 0.1483768178395124 1104 38-39 0.12040291464711572 1104 40-41 0.019284203259182675 1104 42-43 0.07384610394181124 1104 44-45 0.11187939127116664 1104 46-47 0.31368409591495094 1104 48-49 0.32259341409874054 1104 50-51 0.16561141495376575 1104 52-53 0.13242587609970968 1104 54-55 0.1445244828346972 1104 56-57 0.0763051032324924 1104 58-59 0.18034713785222323 1104 60-61 0.2798373879338172 1104 62-63 0.34030099189865837 1104 64-65 0.5190897621327011 1104 66-67 0.21063200769552282 1104 68-69 0.17679197719696305 1104 70-71 0.21473286806812553 1104 72-73 0.17381630407122373 1104 74-75 -0.013340277227669617 1104 76-77 0.07703733191314655 1104 78-79 0.20367915694969696 1104 80-81 0.25657471718238867 1104 82-83 0.19909181401988718 1104 84-85 0.07861423209477181 1104 86-87 0.18716685190203464 1104 88-89 0.18710156195032823 1104 90-91 0.19510361957004108 1104 92-93 0.17394560219458555 1104 94-95 0.262167781110044 1104 96-97 0.44536028012718987 1104 98-99 0.307144737215566 1104 100-101 0.22246797734747403 1105 1 -0.04656658008650538 1105 2 0.070393095149619 1105 3 0.06231757242907321 1105 4 0.0041717507394807285 1105 5 -0.11822836747802512 1105 6 -0.12402319605475043 1105 7 0.0589395661764911 1105 8 0.08411634480987118 1105 9 0.17302142293294054 1105 10-11 0.14995682949415823 1105 12-13 0.11839604207735732 1105 14-15 0.15468638751659824 1105 16-17 0.19664601462940112 1105 18-19 0.17318495205523732 1105 20-21 0.11091146770450422 1105 22-23 0.17289237629437082 1105 24-25 0.2159655522711006 1105 26-27 0.20816862465537156 1105 28-29 0.10318473185237309 1105 30-31 0.12768628720594677 1105 32-33 0.12388253363806712 1105 34-35 0.08942835601339283 1105 36-37 0.1782709781837255 1105 38-39 0.15450767045228275 1105 40-41 0.1357702281549038 1105 42-43 0.16764598570356526 1105 44-45 0.20421196919345874 1105 46-47 0.30112949283417834 1105 48-49 0.23727835842822742 1105 50-51 0.12705260784633055 1105 52-53 0.13542411894794526 1105 54-55 0.30436371165856 1105 56-57 0.39400512293887147 1105 58-59 0.2562298192329848 1105 60-61 0.3221465645357853 1105 62-63 0.31778481624917276 1105 64-65 0.36171383018537284 1105 66-67 0.2902350884588145 1105 68-69 0.24100889511991141 1105 70-71 0.23686805380071263 1105 72-73 0.1888695112837624 1105 74-75 0.20921654282027902 1105 76-77 0.19928049215882027 1105 78-79 0.09249948407551756 1105 80-81 0.06348837041586819 1105 82-83 0.022978403831693583 1105 84-85 0.06728594030904844 1105 86-87 0.1930414044031039 1105 88-89 0.20912015163075637 1105 90-91 0.2578503598071791 1105 92-93 0.1308769090556865 1105 94-95 0.0973013739106463 1105 96-97 0.18217384139759218 1105 98-99 0.09822472867614351 1105 100-101 0.1057787304594413 1106 1 0.16012185609559992 1106 2 0.12096840722288249 1106 3 0.10537818141280297 1106 4 0.23334023864993725 1106 5 0.1992924248459289 1106 6 0.11526374555189989 1106 7 0.0103620957409305 1106 8 0.20255459183383806 1106 9 0.23002396714771578 1106 10-11 0.1581739046720898 1106 12-13 0.12896892750027433 1106 14-15 0.12507691996287917 1106 16-17 0.20688165594334862 1106 18-19 0.20927968034575173 1106 20-21 0.19691380241344092 1106 22-23 0.23366188068494864 1106 24-25 0.20588628671717402 1106 26-27 0.11175298316054949 1106 28-29 0.07887376490003817 1106 30-31 0.20987022730234628 1106 32-33 0.2853529405617081 1106 34-35 0.3061276669049846 1106 36-37 0.22046436111516954 1106 38-39 0.34119150202767656 1106 40-41 0.41849534142370715 1106 42-43 0.2660847150431209 1106 44-45 0.41279763899417077 1106 46-47 0.43545287941314825 1106 48-49 0.33086058419413433 1106 50-51 0.24147369347845427 1106 52-53 0.21439105169204709 1106 54-55 0.3523642874227946 1106 56-57 0.34483022173820643 1106 58-59 0.4054793663084908 1106 60-61 0.4606267202634271 1106 62-63 0.528882295245829 1106 64-65 0.6653703099446986 1106 66-67 0.5644039983954272 1106 68-69 0.4975678220280031 1106 70-71 0.4318388586347126 1106 72-73 0.47414976679045395 1106 74-75 0.49838188814317874 1106 76-77 0.4399814097642434 1106 78-79 0.4346694294834208 1106 80-81 0.45796600755176087 1106 82-83 0.4552111783823065 1106 84-85 0.3789098592438549 1106 86-87 0.38138055818038197 1106 88-89 0.42800024495882383 1106 90-91 0.38247242031970075 1106 92-93 0.32125045494952786 1106 94-95 0.21528500080335533 1106 96-97 0.3332164343865358 1106 98-99 0.37100980733880107 1106 100-101 0.4543129808957893 1107 1 -0.07807885158894834 1107 2 -0.03505151922661298 1107 3 -0.022327755660647597 1107 4 0.11869290724665404 1107 5 0.02441783454659685 1107 6 -0.001993846407970068 1107 7 0.024441714599767295 1107 8 0.15022697040159727 1107 9 0.265505218723483 1107 10-11 0.2192616871156048 1107 12-13 0.1157148826570662 1107 14-15 0.0927985060804346 1107 16-17 0.15690382542249637 1107 18-19 0.232841750540409 1107 20-21 0.1476740851394993 1107 22-23 0.15866835765042708 1107 24-25 0.20874867076210535 1107 26-27 0.21531522723347507 1107 28-29 0.2541373208844888 1107 30-31 0.21407147400952908 1107 32-33 0.13688941587534487 1107 34-35 0.19346793798120387 1107 36-37 0.2239153230966906 1107 38-39 0.29893943070280926 1107 40-41 0.23429983269936372 1107 42-43 0.38575573736039814 1107 44-45 0.12733264503643937 1107 46-47 0.4545167188191783 1107 48-49 0.19845871674154125 1107 50-51 -0.11409588825737416 1107 52-53 0.15204508610255374 1107 54-55 0.2066188278190637 1107 56-57 0.27753272471587564 1107 58-59 0.3312309116515948 1107 60-61 0.28676549250898375 1107 62-63 0.3697781160816618 1107 64-65 0.5234489550852359 1107 66-67 0.2872844657802105 1107 68-69 0.23650212212450583 1107 70-71 0.2623032005489492 1107 72-73 0.34622039614284006 1107 74-75 0.3558185747189029 1107 76-77 0.31128853964261083 1107 78-79 0.28736202139982225 1107 80-81 0.31633885472145806 1107 82-83 0.290941771394138 1107 84-85 0.34060343357247547 1107 86-87 0.3180335754030281 1107 88-89 0.28945734484319985 1107 90-91 0.2669137929147354 1107 92-93 0.18833321231109323 1107 94-95 0.2005536861967201 1107 96-97 0.06717329518829018 1107 98-99 0.027124845200788172 1107 100-101 0.0965913078390912 1108 1 0.09496086232032752 1108 2 0.08282538647637949 1108 3 0.03220264147844176 1108 4 0.029191683832387127 1108 5 0.06951476660005085 1108 6 0.15044733559842882 1108 7 0.13532445504772284 1108 8 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-0.9884648754822294 2316 56-57 -0.9957978611760154 2316 58-59 -1.3304801199502236 2316 60-61 -0.7600907476173973 2316 62-63 -0.7786722645014166 2316 64-65 -0.9426210172530176 2316 66-67 -1.3630158170813829 2316 68-69 -1.6118623384715676 2316 70-71 -1.3236667399439739 2316 72-73 -1.1130415411780348 2316 74-75 -1.1959814365206327 2316 76-77 -0.8217730467550552 2316 78-79 -1.1301796429252775 2316 80-81 -1.0067762443425394 2316 82-83 -1.129045990003057 2316 84-85 -1.482426619690429 2316 86-87 -1.029938125950089 2316 88-89 -0.8054913591761874 2316 90-91 -0.9177286338253552 2316 92-93 -0.95783991396506 2316 94-95 -1.0348756951918645 2316 96-97 -0.84133353282812 2316 98-99 -0.5551651472437413 2316 100-101 -1.1140040752104419 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 8.0 7 106.0 8 386.0 9 565.0 10 602.0 11 734.0 12 989.0 13 1116.0 14 1349.0 15 1762.0 16 2609.0 17 4090.0 18 5404.0 19 7200.0 20 9289.0 21 11194.0 22 10736.0 23 8767.0 24 7505.0 25 6896.0 26 7642.0 27 8647.0 28 10573.0 29 12989.0 30 16702.0 31 20682.0 32 27206.0 33 37141.0 34 54279.0 35 83048.0 36 139415.0 37 315110.0 38 716013.0 39 262046.0 40 87.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.168572573436485 27.32596750104379 14.271183306106094 21.234276619413627 2 12.946757726184352 23.792387434771875 43.65129706068252 19.609557778361253 3 14.079212546848924 31.770920077663305 34.869180701317084 19.280686674170692 4 8.87618689816492 19.95694069869894 48.34661675321016 22.82025564992598 5 8.490547573606014 44.13598386374324 38.458880113808256 8.914588448842485 6 22.23276714901323 47.064840121397076 20.67059027370233 10.031802455887366 7 22.35852915928966 36.56624997764544 28.094541910331383 12.980678952733516 8 21.778499597050477 39.370851045042045 24.06916424580256 14.781485112104917 9 25.56421399000331 16.72864302511691 25.61585164122786 32.091291343651925 10-11 20.107376990996116 29.09714680482048 35.06344191017133 15.732034294012074 12-13 20.779479894048457 29.646061593067934 32.13107186379543 17.44338664908818 14-15 19.84532293880827 31.094733970992884 29.294965701532625 19.764977388666217 16-17 17.73289342198552 33.652129494716036 30.550335795273742 18.064641288024696 18-19 17.084193998721137 33.04567301522471 33.37367440791243 16.49645857814172 20-21 18.06640981770975 32.76201870451633 32.495938802459165 16.675632675314752 22-23 18.796667051520817 31.677121871038167 31.679046141781384 17.847164935659638 24-25 18.602315706455567 32.288593759673645 31.191564220165574 17.917526313705213 26-27 18.39212398773598 31.8494974864562 32.87585888011905 16.882519645688767 28-29 18.171446387864933 30.339140168900773 33.9458091893131 17.54360425392119 30-31 18.948823880144147 31.687496200262483 31.68604602520962 17.677633894383753 32-33 17.6840202422127 32.42984638741873 32.47594522131066 17.410188149057916 34-35 18.360136472627666 32.04231499252323 32.06236645142722 17.535182083421876 36-37 19.52727639834524 30.185728381097082 31.498164691918678 18.788830528639007 38-39 18.00155280282583 32.878759230224766 31.905970649572453 17.213717317376943 40-41 17.019114980475624 31.51871813449481 34.1167067416965 17.34546014333307 42-43 17.528544743756857 31.4046284010091 31.64321008518663 19.423616770047413 44-45 18.433012159296588 31.898443803294146 31.846722318463033 17.821821718946236 46-47 18.368177334119547 30.875380414886273 32.4294852626784 18.32695698831578 48-49 17.167465113818846 31.823471303773065 31.784197919990042 19.22486566241805 50-51 17.43004213182926 30.843523982325728 30.822455277138584 20.903978608706424 52-53 17.740141722205834 31.608137626433525 32.19317220579219 18.458548445568454 54-55 18.5064549936713 30.857953855621933 31.069543119581006 19.566048031125753 56-57 18.768461821093577 31.59778720590912 30.393029486424943 19.240721486572358 58-59 18.3358465053882 30.450070873126776 30.86958730569905 20.344495315785977 60-61 19.825908174647015 32.092933499480836 29.938303496972985 18.142854828899164 62-63 18.153618638535914 31.879215175400216 31.61263088447222 18.35453530159165 64-65 17.899600784580052 31.059803006067536 31.24731920255212 19.793277006800288 66-67 18.898746128587565 31.090449929732504 30.429315700109377 19.58148824157055 68-69 18.29139561328349 30.883516073636624 30.793032333210213 20.03205597986967 70-71 18.3586752213333 30.080617293668354 32.913890056567766 18.64681742843059 72-73 19.27076588866215 30.138251342939686 30.86017910327798 19.730803665120185 74-75 17.94825678460137 31.086061258721102 30.099964196764752 20.865717759912776 76-77 17.726161540024478 31.82727127143577 31.580741924493992 18.865825264045757 78-79 17.999095181843316 30.90842067341929 30.72608864425287 20.366395500484526 80-81 18.90985070994209 30.968469254264242 30.132074265712134 19.989605770081532 82-83 18.64666418240613 31.637923214019043 30.60664804866918 19.108764554905648 84-85 18.238938581764522 30.385464233691568 30.732483098497976 20.643114086045937 86-87 17.28557514393599 31.189610375112682 31.883991638735314 19.640822842216014 88-89 19.079665256689296 30.030703506407985 30.841935981835274 20.047695255067445 90-91 18.02639264418959 30.22930508230391 32.353632618234734 19.390669655271772 92-93 19.353263585551467 31.38373636221172 30.758458941447564 18.504541110789248 94-95 18.664549963139198 29.836500011169942 31.44435694658535 20.054593079105512 96-97 19.65574183233304 29.00224473720497 30.957415867015094 20.384597563446903 98-99 18.266323600950827 30.696455220183605 30.50270626156333 20.53451491730224 100-101 18.315478399669598 30.363303018548855 31.354956453309747 19.966262128471808 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 386.0 1 244.5 2 193.0 3 387.5 4 332.0 5 238.0 6 429.0 7 805.5 8 1105.5 9 1109.0 10 904.0 11 769.5 12 790.5 13 830.5 14 922.0 15 971.5 16 1007.0 17 1078.5 18 1183.5 19 1290.0 20 1504.0 21 1822.0 22 2163.5 23 2579.0 24 2913.5 25 3761.0 26 6268.5 27 10982.0 28 14952.0 29 19637.5 30 30551.5 31 43150.5 32 69238.5 33 98941.5 34 131066.5 35 156175.5 36 173337.5 37 184394.5 38 160353.5 39 130727.0 40 101495.5 41 74576.0 42 59539.0 43 50967.5 44 45080.5 45 37739.5 46 30134.0 47 22845.0 48 23343.5 49 20688.5 50 13870.5 51 9761.0 52 6367.5 53 5206.5 54 4410.5 55 3516.5 56 3180.5 57 3067.0 58 2993.5 59 2868.0 60 2720.0 61 2582.5 62 2029.5 63 1239.5 64 654.5 65 504.0 66 366.0 67 255.5 68 306.0 69 302.0 70 202.5 71 146.5 72 118.5 73 124.0 74 116.0 75 88.0 76 56.0 77 32.0 78 21.0 79 12.0 80 8.5 81 9.0 82 7.0 83 8.0 84 6.5 85 4.5 86 4.0 87 2.0 88 0.5 89 1.0 90 2.0 91 1.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47532276155719794 2 0.37124481353258737 3 0.2021878679470597 4 0.23375706332858678 5 0.25746184784651793 6 0.20731925659564715 7 0.19761423893418828 8 0.19973372554990915 9 0.19493699268275133 10-11 0.21027538266494206 12-13 0.19959428564098017 14-15 0.20860210375779398 16-17 0.09018973309528153 18-19 0.03731411962940219 20-21 2.50991836072212E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.14102952389079734 46-47 0.20759813641350514 48-49 0.08910210180563527 50-51 0.06302683883591102 52-53 0.07158844924415203 54-55 0.08056837937918006 56-57 0.11316943008678182 58-59 0.09247654760171724 60-61 0.1399976685647227 62-63 0.2417609141011118 64-65 0.13246791348255635 66-67 0.10452415573318341 68-69 0.10918144869141223 70-71 0.08834912629741863 72-73 0.07222987282522546 74-75 0.14200560325330042 76-77 0.21476534773245606 78-79 0.1378781819490018 80-81 0.08413804104776264 82-83 0.08977141336849451 84-85 0.09894655937602315 86-87 0.07206254493451066 88-89 0.0961298732156572 90-91 0.11629288404679157 92-93 0.1651805161173013 94-95 0.13202170577398353 96-97 0.08846067822456183 98-99 0.07772380523702832 100-101 0.06313839076305422 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1792887.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 9.82998588648804 #Duplication Level Percentage of deduplicated Percentage of total 1 71.07580510173665 6.986741610208458 2 10.427263401467348 2.049997041422346 3 4.260019651386349 1.256277991478685 4 2.303969829486123 0.9059196362697136 5 1.5193978197757314 0.7467829562178069 6 1.00707604724668 0.5939725998633015 7 0.8045846608287603 0.5536339102302035 8 0.6451939788516815 0.5073798164927272 9 0.5158321051955577 0.4563560082482771 >10 4.744763118938901 10.0679376060995 >50 1.0552480238326338 7.402931910647113 >100 1.3070552659236612 28.075683886970705 >500 0.2008601954354013 13.889498938005406 >1k 0.1288316122326189 23.390013610268266 >5k 0.0035135894245259705 2.345799695891856 >10k+ 5.855982374209951E-4 0.7710727816856375 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 13395 0.7471190320416178 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 8503 0.4742630182493375 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 7335 0.4091166927977056 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 7285 0.4063278946191255 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 6014 0.33543664491961844 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 5878 0.3278511138738805 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 5736 0.31993092704671294 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 4989 0.27826628225872574 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4858 0.27095963103084575 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 4714 0.262927892276535 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 4522 0.2522189072707873 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 4503 0.25115916396292687 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 4443 0.24781260614863068 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 4149 0.23141447285857947 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4047 0.22572532457427602 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 4026 0.22455402933927235 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 3862 0.2154067713135295 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 3841 0.21423547607852586 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 3831 0.2136777164428098 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 3712 0.20704037677778914 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 3616 0.20168588427491524 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 3492 0.19476966479203653 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 3415 0.19047491559702312 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 3371 0.1880207731998726 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 3337 0.18612439043843812 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 3289 0.18344714418700117 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 3245 0.18099300178985067 No Hit ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC 3123 0.17418833423411514 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 3100 0.1729054870719683 No Hit ACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTTGATTCATAAA 2977 0.16604504355266114 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 2973 0.16582193969837475 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 2934 0.16364667711908223 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 2891 0.16124831068550333 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 2850 0.15896149617906763 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 2792 0.15572649029191465 No Hit TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA 2758 0.15383010753048015 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 2745 0.15310502000404935 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 2741 0.15288191614976293 No Hit GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT 2729 0.1522126045869037 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 2710 0.15115286127904323 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 2635 0.14696966401117306 No Hit TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC 2615 0.145854144739741 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 2613 0.14574259281259777 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2612 0.14568681684902618 No Hit GATGAAAGACTAATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACC 2604 0.14524060914045336 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 2601 0.14507328124973856 No Hit AACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAACAAG 2556 0.14256336288901642 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 2545 0.1419498272897288 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 2445 0.13637223093256853 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 2435 0.1358144712968525 No Hit GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCT 2416 0.13475472798899207 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 2404 0.13408541642613284 No Hit ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG 2404 0.13408541642613284 No Hit CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC 2360 0.1316312740289823 No Hit CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC 2312 0.12895402777754536 No Hit GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG 2298 0.12817316428754294 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 2286 0.1275038527246837 No Hit GTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACCGC 2236 0.12471505454610357 No Hit TTTCACCTCTACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTT 2231 0.12443617472824556 No Hit TTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGT 2208 0.1231533275660987 No Hit ACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAG 2198 0.12259556793038266 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 2196 0.12248401600323947 No Hit CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA 2150 0.11991832167894574 No Hit ACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTC 2150 0.11991832167894574 No Hit GATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGT 2146 0.11969521782465933 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 2131 0.1188585783710853 No Hit TCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAA 2125 0.11852392258965568 No Hit CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC 2106 0.11746417928179523 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 2098 0.11701797157322241 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 2069 0.11540046862964592 No Hit CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG 2058 0.1147869330303583 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 2045 0.11406184550392746 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 2027 0.11305787815963861 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 2025 0.11294632623249541 No Hit GGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTA 2023 0.1128347743053522 No Hit GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA 2009 0.11205391081534977 No Hit GTACAAGGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCT 1989 0.11093839154391771 No Hit CTACTATACAATCAAAGATATCCTAGGTATCCTAATCATATTCTTAATTC 1970 0.10987864823605728 No Hit AATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACCTTTTGCATAAT 1968 0.10976709630891406 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 1946 0.10854002511033879 No Hit CTATTTTGCCACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCT 1939 0.10814959336533758 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 1935 0.10792648951105116 No Hit GTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTAT 1934 0.10787071354747955 No Hit ATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAA 1923 0.10725717794819194 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1901 0.10603010674961669 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 1875 0.10457993169675502 No Hit GTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG 1873 0.10446837976961179 No Hit ATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTAT 1871 0.10435682784246861 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 1856 0.10352018838889457 No Hit CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT 1838 0.10251622104460571 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 1812 0.10106604599174403 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 5.577596357160267E-5 0.0 6 0.0 0.0 0.0 5.577596357160267E-5 0.0 7 0.0 0.0 0.0 5.577596357160267E-5 0.0 8 0.0 0.0 0.0 5.577596357160267E-5 0.0 9 0.0 0.0 0.0 5.577596357160267E-5 0.0 10-11 0.0 0.0 0.0 5.577596357160267E-4 0.0 12-13 0.0 0.0 0.0 0.0013107351439326628 0.0 14-15 0.0 0.0 0.0 0.0022589265246499082 0.0 16-17 0.0 0.0 0.0 0.006219019938233698 0.0 18-19 0.0 0.0 0.0 0.00939824986181505 0.0 20-21 0.0 0.0 0.0 0.01687222898040981 0.0 22-23 0.0 0.0 0.0 0.037007351829758374 0.0 24-25 0.0 0.0 0.0 0.059568729094471654 0.0 26-27 0.0 0.0 0.0 0.07158844924415203 0.0 28-29 0.0 0.0 0.0 0.08235321021347133 0.0 30-31 0.0 0.0 0.0 0.10187479746353229 0.0 32-33 0.0 0.0 0.0 0.13748775020400059 0.0 34-35 0.0 0.0 0.0 0.19426768111989212 0.0 36-37 0.0 0.0 0.0 0.2789913697851566 0.0 38-39 0.0 0.0 0.0 0.40459883974840577 0.0 40-41 0.0 0.0 0.0 0.5575086438799545 0.0 42-43 0.0 0.0 0.0 0.6937135469218082 0.0 44-45 0.0 0.0 0.0 0.8493563732683655 0.0 46-47 0.0 0.0 0.0 1.0374050344500239 0.0 48-49 0.0 0.0 0.0 1.2391745826702967 0.0 50-51 0.0 0.0 0.0 1.467075169823865 0.0 52-53 0.0 0.0 0.0 1.6732231311845087 0.0 54-55 0.0 0.0 0.0 1.8732357365522758 0.0 56-57 0.0 0.0 0.0 2.1155544102891035 0.0 58-59 0.0 0.0 0.0 2.388828743808171 0.0 60-61 0.0 0.0 0.0 2.6899631711312537 0.0 62-63 0.0 0.0 0.0 2.9657753109928287 0.0 64-65 0.0 0.0 0.0 3.2484200063919255 0.0 66-67 0.0 0.0 0.0 3.5630522168993357 0.0 68-69 0.0 0.0 0.0 3.908835303061487 0.0 70-71 0.0 0.0 0.0 4.344529242501061 0.0 72-73 0.0 0.0 0.0 4.7509408010655445 0.0 74-75 0.0 0.0 0.0 5.1212653111992 0.0 76-77 0.0 0.0 0.0 5.549011175829821 0.0 78-79 5.577596357160267E-5 0.0 0.0 6.0275131673106 0.0 80-81 5.577596357160267E-5 0.0 0.0 6.578998007124822 0.0 82-83 5.577596357160267E-5 0.0 0.0 7.1068617263664695 0.0 84-85 5.577596357160267E-5 0.0 0.0 7.599893356357651 0.0 86-87 5.577596357160267E-5 0.0 0.0 8.124745173566433 0.0 88-89 5.577596357160267E-5 0.0 0.0 8.707687656835038 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGGCTC 25 0.0050202473 57.00426 7 ATGTATT 120 0.0 55.551178 1 GGTAATC 155 0.0 45.973743 8 GGGCATC 65 2.5888603E-6 43.849426 7 GGCATCT 55 4.9606002E-5 43.187458 8 GACATAT 475 0.0 43.104374 1 CATGGGT 740 0.0 42.389347 4 CTTTGCG 45 9.553049E-4 42.288 2 TAACTTT 3410 0.0 41.237057 6 TGGGATA 295 0.0 40.25949 6 ACATATA 515 0.0 39.721977 2 CATTCCG 635 0.0 39.650845 9 GAACCTC 60 8.28623E-5 39.588505 9 CGTCTAG 50 0.0015969222 38.09224 1 ACCTTAA 125 1.3096724E-10 38.09224 1 ATGGGTA 675 0.0 38.02196 5 TGGGTAT 240 0.0 37.609077 6 AACTTTA 3780 0.0 37.198547 7 TAGGCAT 660 0.0 36.725758 5 CCTTTAG 705 0.0 36.471294 1 >>END_MODULE