##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031643_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1792887 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.44212267700084 34.0 31.0 34.0 31.0 34.0 2 32.66332735972763 34.0 31.0 34.0 31.0 34.0 3 32.74738787218603 34.0 31.0 34.0 31.0 34.0 4 36.22458972595596 37.0 37.0 37.0 35.0 37.0 5 35.97966743023961 37.0 35.0 37.0 35.0 37.0 6 36.052633545783976 37.0 37.0 37.0 35.0 37.0 7 35.986505563373484 37.0 36.0 37.0 35.0 37.0 8 36.08905915431369 37.0 37.0 37.0 35.0 37.0 9 37.945159957097125 39.0 39.0 39.0 35.0 39.0 10-11 37.90777779079217 39.0 38.0 39.0 35.0 39.0 12-13 37.953801606013094 39.0 38.0 39.0 35.0 39.0 14-15 39.36225512260393 41.0 39.0 41.0 36.0 41.0 16-17 39.32850118272931 41.0 39.0 41.0 36.0 41.0 18-19 39.41023639526641 41.0 39.0 41.0 36.0 41.0 20-21 39.44582675874163 41.0 39.0 41.0 36.0 41.0 22-23 39.354070557709434 41.0 39.0 41.0 36.0 41.0 24-25 39.28951573635148 41.0 39.0 41.0 36.0 41.0 26-27 39.17639873567046 41.0 39.0 41.0 36.0 41.0 28-29 39.09791442517013 41.0 39.0 41.0 35.5 41.0 30-31 38.96502930748006 40.0 38.5 41.0 35.0 41.0 32-33 38.80413182760542 40.0 38.0 41.0 35.0 41.0 34-35 38.726230375924416 40.0 38.0 41.0 35.0 41.0 36-37 38.57201736640401 40.0 38.0 41.0 35.0 41.0 38-39 38.405688702076596 40.0 38.0 41.0 34.0 41.0 40-41 38.35869522172898 40.0 38.0 41.0 34.0 41.0 42-43 38.24961472753163 40.0 38.0 41.0 34.0 41.0 44-45 38.15506470848413 40.0 38.0 41.0 34.0 41.0 46-47 38.10705303792152 40.0 38.0 41.0 33.0 41.0 48-49 38.18414406485183 40.0 38.0 41.0 34.0 41.0 50-51 38.1402160314621 40.0 38.0 41.0 33.0 41.0 52-53 38.10742506359854 40.0 38.0 41.0 33.0 41.0 54-55 38.02543579154738 40.0 38.0 41.0 33.0 41.0 56-57 37.94935626171644 40.0 38.0 41.0 33.0 41.0 58-59 37.826153851302394 40.0 38.0 41.0 33.0 41.0 60-61 37.611804313378364 40.0 38.0 41.0 33.0 41.0 62-63 37.43578401762074 40.0 37.0 41.0 32.0 41.0 64-65 37.220156931251104 40.0 37.0 41.0 32.0 41.0 66-67 36.973443669344476 39.0 36.5 41.0 31.0 41.0 68-69 36.67478513704433 39.0 36.0 41.0 31.0 41.0 70-71 36.32159416628042 38.5 36.0 40.0 31.0 41.0 72-73 35.97354434495872 37.5 35.0 40.0 31.0 41.0 74-75 35.545504262120254 37.0 35.0 39.0 30.0 41.0 76-77 34.85603164058861 36.0 34.5 39.0 29.5 40.0 78-79 34.75660122472861 36.0 35.0 38.5 30.0 40.0 80-81 34.40016102520683 36.0 34.5 37.0 29.5 39.0 82-83 34.04880368924533 35.0 34.0 37.0 29.0 39.0 84-85 33.68803220727241 35.0 34.0 36.5 29.0 38.0 86-87 33.41037109421843 35.0 34.0 36.0 29.0 37.0 88-89 33.13993770940388 35.0 34.0 36.0 29.0 37.0 90-91 32.97155509521794 35.0 34.0 35.5 29.0 36.5 92-93 32.832871508354955 35.0 34.0 35.0 29.0 36.0 94-95 32.67307002616451 35.0 34.0 35.0 28.0 36.0 96-97 32.557078332321 35.0 34.0 35.0 27.0 36.0 98-99 32.49459475137027 35.0 34.0 35.0 27.0 35.5 100-101 32.09724595024672 34.5 33.0 35.0 26.0 35.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2917042262485623 1101 2 0.2544808082873615 1101 3 0.28330407600598306 1101 4 0.1992078543920357 1101 5 0.2583535034371067 1101 6 0.26377158134962997 1101 7 0.37319112353811335 1101 8 0.2629249821674051 1101 9 0.32051553432125957 1101 10-11 0.04722296082486821 1101 12-13 0.15074957508790732 1101 14-15 0.17254650097694224 1101 16-17 0.23348388278585475 1101 18-19 0.18609545116462556 1101 20-21 0.13885983195149976 1101 22-23 0.2698957802953288 1101 24-25 0.2749881684824018 1101 26-27 0.322909229035254 1101 28-29 0.20918365027636554 1101 30-31 0.1501743085654681 1101 32-33 0.15501327226080974 1101 34-35 0.13087962056502533 1101 36-37 0.1962372589385808 1101 38-39 0.24796135394088736 1101 40-41 0.2167356232185469 1101 42-43 0.24061209799990735 1101 44-45 0.31849139513486335 1101 46-47 0.3332740799898488 1101 48-49 0.48285551439662555 1101 50-51 0.46082583004292843 1101 52-53 0.3260857776943169 1101 54-55 0.4387037937934437 1101 56-57 0.4745785873199466 1101 58-59 0.32876325442234844 1101 60-61 0.2900835773675965 1101 62-63 0.21694718477078112 1101 64-65 0.409688530574833 1101 66-67 0.6330757676951109 1101 68-69 0.5657505918599739 1101 70-71 0.5483051100207561 1101 72-73 0.5553910765897214 1101 74-75 0.4705695423623695 1101 76-77 0.40527375104543495 1101 78-79 0.6157433685390998 1101 80-81 0.5843102582731063 1101 82-83 0.493789204664445 1101 84-85 0.49420907732366715 1101 86-87 0.3621209166050221 1101 88-89 0.46056257395569133 1101 90-91 0.5256835270249809 1101 92-93 0.3610587669028362 1101 94-95 0.42163181463518384 1101 96-97 0.26405395582670366 1101 98-99 0.3297528523279567 1101 100-101 0.32386468376660105 1102 1 0.0999984575354631 1102 2 0.17974778498022914 1102 3 0.15718226025057191 1102 4 0.1604185926240902 1102 5 -0.08791101061157036 1102 6 -0.03177045243872101 1102 7 0.12319611731253843 1102 8 0.0935252148297323 1102 9 0.1328247602481767 1102 10-11 0.1453249460642283 1102 12-13 0.11760684540519861 1102 14-15 0.1622172352710507 1102 16-17 0.1978055046876861 1102 18-19 0.2172929868082818 1102 20-21 0.16800326171382807 1102 22-23 0.15900140956322417 1102 24-25 0.1281269697292231 1102 26-27 0.09634525022407558 1102 28-29 -0.1817183109928706 1102 30-31 -0.24534808304347422 1102 32-33 0.1568854620737099 1102 34-35 0.1540428865505703 1102 36-37 0.07751193650720722 1102 38-39 0.11882662761399843 1102 40-41 0.27850789949506805 1102 42-43 0.2849767900171898 1102 44-45 0.3288301727920597 1102 46-47 0.3298466861408471 1102 48-49 0.20125538541456223 1102 50-51 0.2824826883024869 1102 52-53 0.15538073284071174 1102 54-55 -0.018929023960417624 1102 56-57 0.031133796112030154 1102 58-59 0.14656896140287046 1102 60-61 0.30012225296154327 1102 62-63 0.14578399678323706 1102 64-65 0.2253121959291704 1102 66-67 0.313428309145813 1102 68-69 0.19597443660971692 1102 70-71 0.3047611091608786 1102 72-73 0.31621425036641426 1102 74-75 0.3291584539002699 1102 76-77 0.21097954258721785 1102 78-79 0.028530665137907363 1102 80-81 0.10952430095704102 1102 82-83 -0.1461187576948646 1102 84-85 0.14893214161779866 1102 86-87 0.12990660092809492 1102 88-89 0.22467355369618502 1102 90-91 0.2899257716932908 1102 92-93 0.2911119196866565 1102 94-95 0.20753586377772848 1102 96-97 0.340299379389144 1102 98-99 0.1968221440395297 1102 100-101 0.1447085079895416 1103 1 0.152638533965046 1103 2 0.20566997482305993 1103 3 0.23495816998680397 1103 4 0.14572155338098014 1103 5 0.10268793688045008 1103 6 0.14680631339426498 1103 7 0.22643120945146933 1103 8 0.15835559665742238 1103 9 0.23409796399280225 1103 10-11 0.26592548583928277 1103 12-13 0.18240720513663433 1103 14-15 0.2635740561134128 1103 16-17 0.28255009893127436 1103 18-19 0.21503554755521748 1103 20-21 0.1469273053655158 1103 22-23 0.07910527267314649 1103 24-25 0.19667408707425693 1103 26-27 0.23182273943391607 1103 28-29 0.22477509037275922 1103 30-31 0.16827701169292197 1103 32-33 0.36888971942934035 1103 34-35 0.3218993177728322 1103 36-37 0.3119157910775101 1103 38-39 0.4598209327559175 1103 40-41 0.4368951391701401 1103 42-43 0.3643581035528385 1103 44-45 0.546875543073476 1103 46-47 0.5245524340157246 1103 48-49 0.18339312768176086 1103 50-51 0.30693157156946427 1103 52-53 0.40449808321058356 1103 54-55 0.38876993135958315 1103 56-57 0.4221039946225815 1103 58-59 0.5397289938534229 1103 60-61 0.5216501864094525 1103 62-63 0.2722506317821498 1103 64-65 0.37485826183367266 1103 66-67 0.48127695686164174 1103 68-69 0.5829463590557395 1103 70-71 0.4927659251498895 1103 72-73 0.47994186500189784 1103 74-75 0.4848546018206932 1103 76-77 0.42615947056977177 1103 78-79 0.45578868259431005 1103 80-81 0.6078873183600635 1103 82-83 0.4259327166346836 1103 84-85 0.34448606930858006 1103 86-87 0.3783639099519789 1103 88-89 0.5105357260327992 1103 90-91 0.549411197411068 1103 92-93 0.3231377128926809 1103 94-95 0.31532386241138255 1103 96-97 0.2821959485974119 1103 98-99 0.2293645396871682 1103 100-101 0.2786717716182352 1104 1 0.14197093359156554 1104 2 0.11471136915857727 1104 3 0.12695163403909504 1104 4 0.11036518861285316 1104 5 0.041343399065326025 1104 6 0.15208175690500525 1104 7 0.17090677745644456 1104 8 0.13172160972931835 1104 9 0.2021418482131594 1104 10-11 0.06545474198617995 1104 12-13 0.036340445099277474 1104 14-15 0.10371566831384627 1104 16-17 0.18034810702340565 1104 18-19 0.12507678627292051 1104 20-21 0.07149935640815386 1104 22-23 0.18335363868621357 1104 24-25 0.08137296662606985 1104 26-27 0.10178850351732649 1104 28-29 -0.12098432450429897 1104 30-31 -0.05211281090278419 1104 32-33 0.09909902537393833 1104 34-35 0.1518168403374176 1104 36-37 0.14033549229132802 1104 38-39 0.07614913070175788 1104 40-41 0.0601210962196248 1104 42-43 0.14059915552918767 1104 44-45 0.0069984811375860545 1104 46-47 0.16917972937832104 1104 48-49 0.30414865211375997 1104 50-51 0.49370016789688265 1104 52-53 0.4282725930145048 1104 54-55 0.4070044473882106 1104 56-57 0.4980571536639715 1104 58-59 0.30828641682261804 1104 60-61 0.3624453933811296 1104 62-63 0.38710046060256786 1104 64-65 0.3733876735983799 1104 66-67 0.2126923007769861 1104 68-69 0.26500362640712183 1104 70-71 0.2856191775619621 1104 72-73 0.24257803057923866 1104 74-75 0.2531124605197306 1104 76-77 0.2576759660568513 1104 78-79 0.29602366547635484 1104 80-81 0.3976437764988745 1104 82-83 0.4750184620439626 1104 84-85 0.5113278060032584 1104 86-87 0.3762124938299749 1104 88-89 0.4368857104710173 1104 90-91 0.32804876354863666 1104 92-93 0.35679006272150104 1104 94-95 0.4185801649324006 1104 96-97 0.4555502903341093 1104 98-99 0.39707773607683805 1104 100-101 0.3845191105538106 1105 1 0.06952455135357383 1105 2 0.08767258044375836 1105 3 5.918941632430119E-4 1105 4 0.08948135769355048 1105 5 0.0975032834545928 1105 6 0.013979480818690604 1105 7 0.1500705702767604 1105 8 0.13630087862280504 1105 9 0.19307937900436656 1105 10-11 0.17576569216189597 1105 12-13 0.14110446125850729 1105 14-15 0.16859685529450275 1105 16-17 0.08861082584627411 1105 18-19 0.052069593446042006 1105 20-21 0.12827917051134818 1105 22-23 0.07327752281273092 1105 24-25 0.03692195338995674 1105 26-27 0.09278761673044755 1105 28-29 -0.0010049340881721491 1105 30-31 -0.02908878554724481 1105 32-33 0.12843812282196865 1105 34-35 0.16130135441105153 1105 36-37 0.28527464885847564 1105 38-39 0.18098129807457042 1105 40-41 0.17732845655068985 1105 42-43 0.28384807988955174 1105 44-45 0.1928850216834519 1105 46-47 0.28126589550306136 1105 48-49 0.32899880155376593 1105 50-51 0.4298194899975769 1105 52-53 0.27918401285882055 1105 54-55 0.3435404772105315 1105 56-57 0.1655666784118992 1105 58-59 0.17001335215285707 1105 60-61 0.2810839291974645 1105 62-63 0.2879408553869496 1105 64-65 0.2551974578135727 1105 66-67 0.3938341209891618 1105 68-69 0.3915312810200646 1105 70-71 0.3714732846989577 1105 72-73 0.3568146548772049 1105 74-75 0.3876589675647608 1105 76-77 0.32177012968545426 1105 78-79 0.41010058602681454 1105 80-81 0.3082708497737485 1105 82-83 0.3345066710629183 1105 84-85 0.3030437386074567 1105 86-87 0.387948871798244 1105 88-89 0.4265825607354117 1105 90-91 0.4751244196067006 1105 92-93 0.4097529983144952 1105 94-95 0.20583794073953499 1105 96-97 0.3374224780701738 1105 98-99 0.3078505544169019 1105 100-101 0.2809649837104473 1106 1 0.17573986607957437 1106 2 0.12935745954833777 1106 3 0.10709207653882657 1106 4 0.12346727407810931 1106 5 0.20167290179222874 1106 6 0.047928469915341054 1106 7 0.22701045868098646 1106 8 0.229199761157858 1106 9 0.21218925049740278 1106 10-11 0.15402663545062723 1106 12-13 0.10281538540534285 1106 14-15 0.13846852166474122 1106 16-17 0.16754968631538958 1106 18-19 0.17963528633762138 1106 20-21 0.23738461072984052 1106 22-23 0.19206601385823774 1106 24-25 0.16275884631288307 1106 26-27 0.14785223194810726 1106 28-29 0.1854381164976857 1106 30-31 0.15536471320023537 1106 32-33 0.11781116529889601 1106 34-35 0.028339378966947493 1106 36-37 0.11111368959304002 1106 38-39 0.1693367676841646 1106 40-41 0.0828671352471062 1106 42-43 0.10877806876614926 1106 44-45 0.15944152194119 1106 46-47 0.11649614844744605 1106 48-49 0.19036415411139984 1106 50-51 0.2269699845217943 1106 52-53 0.16383371608401376 1106 54-55 0.20505052444730865 1106 56-57 0.28455789273184706 1106 58-59 0.2423682279113777 1106 60-61 0.2275617818681397 1106 62-63 0.22104876898011128 1106 64-65 0.2069668401180209 1106 66-67 0.09799190752230658 1106 68-69 0.1150692995470024 1106 70-71 0.06840575750087652 1106 72-73 0.18802247110290438 1106 74-75 0.0639338256584665 1106 76-77 -0.04760787913489395 1106 78-79 0.16470443912554344 1106 80-81 0.15930183704363543 1106 82-83 0.20569958072218952 1106 84-85 0.16055678373712823 1106 86-87 0.059903990824693665 1106 88-89 0.16775090998014264 1106 90-91 0.2763457708138475 1106 92-93 0.20601629590623105 1106 94-95 0.12668599474682907 1106 96-97 0.06667179152406533 1106 98-99 0.20064310143514064 1106 100-101 0.376008851821247 1107 1 0.07671375825766091 1107 2 0.09418849334158352 1107 3 0.07556203472110923 1107 4 0.13820832586775111 1107 5 0.1363249279087455 1107 6 0.08913205528522639 1107 7 0.15410086668634193 1107 8 0.05968409907288219 1107 9 0.1505817459113672 1107 10-11 0.1380861713695296 1107 12-13 0.06919637007796808 1107 14-15 0.07285746996204523 1107 16-17 0.16489769497452045 1107 18-19 0.09815436513056142 1107 20-21 0.07535840727486942 1107 22-23 0.1500714562480141 1107 24-25 0.1631319890756302 1107 26-27 0.13727017444657008 1107 28-29 0.1235823137451959 1107 30-31 0.038233878497607066 1107 32-33 0.04875801940634261 1107 34-35 0.10599984687335962 1107 36-37 0.18614111888979323 1107 38-39 0.20187234752471994 1107 40-41 0.22733337243445106 1107 42-43 0.11871225584177836 1107 44-45 0.10027213842658966 1107 46-47 0.21086770891795936 1107 48-49 0.09022140005351531 1107 50-51 0.12955694539049034 1107 52-53 0.159969097703339 1107 54-55 0.12797915611228206 1107 56-57 0.22309835855173077 1107 58-59 0.16836598488172427 1107 60-61 0.30769688528223327 1107 62-63 0.1814426032567269 1107 64-65 0.05246695748584784 1107 66-67 0.338537711405003 1107 68-69 0.35800732063844265 1107 70-71 0.34775614829228374 1107 72-73 0.34338791975229555 1107 74-75 0.21261800186670143 1107 76-77 0.09723550001535841 1107 78-79 0.12271874930698345 1107 80-81 0.15022973841987408 1107 82-83 0.25445296048101795 1107 84-85 0.326758326037357 1107 86-87 0.24501044779417214 1107 88-89 0.09680614241191421 1107 90-91 0.11838692779983973 1107 92-93 0.1689014580042496 1107 94-95 0.31293083273575206 1107 96-97 0.21258069669955404 1107 98-99 0.2797688415045201 1107 100-101 0.4024588661661994 1108 1 0.053314548771496106 1108 2 0.09690635518987989 1108 3 0.061837208822367984 1108 4 0.05532483254948062 1108 5 0.1084913122490434 1108 6 0.10980587421991572 1108 7 0.13728927248344291 1108 8 0.1214571086719829 1108 9 0.10083207529213212 1108 10-11 0.1323681213036494 1108 12-13 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quality scores pass #Quality Count 11 3.0 12 3.0 13 35.0 14 150.0 15 586.0 16 1396.0 17 3048.0 18 4758.0 19 6392.0 20 8553.0 21 11376.0 22 11382.0 23 8837.0 24 7463.0 25 7538.0 26 8557.0 27 10735.0 28 13688.0 29 16816.0 30 20496.0 31 25869.0 32 32609.0 33 41972.0 34 58101.0 35 86086.0 36 144175.0 37 319902.0 38 703356.0 39 238911.0 40 94.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.45629307217991 28.087664964705226 14.7585725844703 20.69746937864457 2 12.943300584876214 24.372761256050396 43.412752153265906 19.271186005807486 3 14.035063255978947 31.969452398774255 35.22255328595317 18.772931059293626 4 8.802740889037391 20.486717543285195 48.27999953143382 22.430542036243594 5 8.226341091212106 44.45612021281877 38.56679199525681 8.750746700712316 6 21.97154644994358 47.50645188458614 20.61524234377292 9.906759321697352 7 22.03864493412022 36.95218940178606 28.078122045616933 12.931043618476792 8 21.59974387677528 39.705012083862506 24.0147873234621 14.680456715900108 9 25.31481348238902 16.534672848874468 25.749140910721092 32.40137275801543 10-11 19.87339414028882 29.204182974164016 35.29645203518125 15.625970850365919 12-13 20.648150162280167 29.72381973877885 32.2979083455901 17.33012175335088 14-15 19.79818025341251 31.113338431256405 29.457907832451234 19.630573482879846 16-17 17.606882084593174 33.85740428705211 30.556861642702525 17.97885198565219 18-19 16.94415208543539 32.96091722456575 33.61363543826242 16.481295251736444 20-21 17.99000717836651 32.5756447561949 32.80608314969097 16.628264915747618 22-23 18.63983677394273 31.684730075170776 31.869575330019057 17.805857820867434 24-25 18.482230056885907 32.14787100358249 31.49861089962725 17.871288039904357 26-27 18.29443238754032 31.532578461442355 33.296298093521784 16.876691057495535 28-29 18.117918645612825 30.119962700462004 34.29805767369933 17.464060980225835 30-31 18.90630996902809 31.44653105670448 31.931750225371974 17.715408748895452 32-33 17.70129277600472 32.20617484881917 32.69580441812809 17.39672795704802 34-35 18.287934487784227 31.805518139179995 32.425774742078 17.48077263095778 36-37 19.516762629211993 30.064331996383487 31.673161777624582 18.745743596779942 38-39 17.931247200743826 32.72306062791464 32.20551546193374 17.140176709407786 40-41 16.854464336012253 31.305849169523793 34.41050662980991 17.429179864654046 42-43 17.497728523883545 31.244997593267172 31.83137029829543 19.42590358455385 44-45 18.455262378499036 31.64070016682591 32.13150075827422 17.772536696400834 46-47 18.338969494452243 30.688520804713292 32.65356377730443 18.318945923530038 48-49 17.059678353934537 31.59543355989219 32.06238863506448 19.28249945110879 50-51 17.363028456276627 30.72237523794914 30.92620613194062 20.98839017383361 52-53 17.64575793120258 31.398158389234794 32.36726575629139 18.58881792327124 54-55 18.447699057490933 30.605246102068524 31.19795367661085 19.749101163829692 56-57 18.717855614994143 31.436392812263126 30.405680893441694 19.440070679301037 58-59 18.209318267130055 30.40874857143813 30.847649628783074 20.53428353264874 60-61 19.83608555254334 32.099717411001755 29.84773436578389 18.21646267067101 62-63 18.06580671286032 31.870999120413057 31.583195148383584 18.47999901834304 64-65 17.772062600710477 31.041777869994036 31.217221163408514 19.968938365886974 66-67 18.714285953325557 31.21192244686921 30.289917881048833 19.783873718756396 68-69 18.261580344996645 30.82715196217051 30.641752659258504 20.269515033574343 70-71 18.204465758299325 30.01329698971547 32.917607188852394 18.864630063132815 72-73 19.05555118643841 30.185867821006006 30.789837842541107 19.968743150014472 74-75 17.756640546782926 31.059765618245876 29.939310174037736 21.244283660933455 76-77 17.376862010823885 31.935420358338256 31.425265507530593 19.26245212330727 78-79 17.625781910161557 30.937539217518133 30.554776240528536 20.881902631791778 80-81 18.505754756396946 30.876933057621198 30.079974741882452 20.5373374440994 82-83 18.171679129494787 31.63602369034325 30.511775448210006 19.680521731951956 84-85 17.737899823022868 30.40211123177311 30.540324069503544 21.319664875700475 86-87 16.732314975790445 31.198508327630243 31.725312303564028 20.343864393015288 88-89 18.468090850120504 29.814288351691992 30.770176815382115 20.947443982805385 90-91 17.338376595959478 30.16682032944631 32.25114020013532 20.243662874458902 92-93 18.562658996356156 31.384381726232608 30.584414968706895 19.46854430870434 94-95 17.80803809721416 29.76699033458327 31.324617781265633 21.100353786936935 96-97 18.868060284892465 28.990365817812275 30.739890467162738 21.40168343013252 98-99 17.257557224744225 30.603852892011602 30.551005166527506 21.58758471671667 100-101 17.095555938550504 30.34368590993186 31.48441591689828 21.07634223461936 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 343.0 1 220.5 2 214.5 3 401.5 4 307.0 5 207.0 6 418.5 7 775.5 8 1077.0 9 1132.5 10 909.5 11 704.5 12 695.5 13 723.5 14 760.5 15 840.0 16 918.0 17 982.0 18 1061.0 19 1165.5 20 1410.5 21 1687.0 22 2002.0 23 2440.5 24 2687.5 25 3367.5 26 5905.0 27 10735.5 28 14531.5 29 19562.5 30 30928.0 31 43385.0 32 69971.0 33 100275.5 34 132457.0 35 158010.0 36 175064.5 37 183849.5 38 158236.5 39 128206.5 40 99832.5 41 73891.5 42 58937.0 43 51146.5 44 45907.5 45 38917.5 46 31078.0 47 23674.0 48 24181.0 49 21125.0 50 14121.5 51 10228.5 52 6716.0 53 5150.5 54 4125.5 55 3210.0 56 2958.0 57 2958.0 58 2873.0 59 2698.0 60 2570.0 61 2488.0 62 1925.0 63 1134.5 64 533.5 65 366.0 66 306.5 67 246.5 68 267.0 69 268.5 70 194.5 71 124.0 72 84.0 73 74.5 74 64.0 75 45.0 76 24.0 77 12.5 78 10.0 79 6.0 80 4.0 81 4.0 82 3.0 83 1.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046684481509431434 2 0.0016175029435764776 3 7.250875264308347E-4 4 0.010262777297174893 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.002007934688577696 24-25 0.0 26-27 0.0 28-29 0.020720770466850395 30-31 0.01645390925362279 32-33 5.577596357160267E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0355571767768967 50-51 0.0125217038218248 52-53 0.0 54-55 7.250875264308347E-4 56-57 0.0 58-59 0.0 60-61 2.7887981785801335E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.1155192714320534E-4 80-81 0.010932088860034123 82-83 0.004964060757872638 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1792887.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 9.097641483254423 #Duplication Level Percentage of deduplicated Percentage of total 1 68.47868670491849 6.229945408854495 2 11.465275216974664 2.0861392686175506 3 4.555798048801776 1.2434105195432579 4 2.5182571303951167 0.9164080213993542 5 1.6019817674042685 0.7287127891277154 6 1.118840322300303 0.610728487757819 7 0.86085097281719 0.5482199464841113 8 0.6925776673133061 0.504065865322009 9 0.5798104288914445 0.47474166692757097 >10 5.211763775570852 10.158640701353095 >50 1.1331757496428008 7.396304518702178 >100 1.413759503071519 27.786527783038046 >500 0.2219162413481681 14.019271997367488 >1k 0.1415672574117624 23.47840810891219 >5k 0.00510152278961306 2.9890955974358167 >10k+ 6.376903487016325E-4 0.829379319157317 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 14296 0.7973731752196319 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 8548 0.4767729366100596 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 8159 0.4550760867807062 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 6959 0.388144930494783 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 5904 0.3293012889267422 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 5792 0.3230543810067227 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 5632 0.31413022683526626 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 5376 0.299851580160936 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5153 0.2874135402844686 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4776 0.26638600201797435 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 4686 0.2613661652965301 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 4672 0.26058530180652767 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 4584 0.2556770170122266 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 4541 0.25327865057864773 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 4192 0.23381283929215838 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 4082 0.2276774832992821 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 4067 0.22684084384570805 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 3992 0.22265764657783788 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 3978 0.22187678308783543 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 3839 0.21412392415138265 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 3835 0.21390082029709626 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 3666 0.20447468245349537 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 3514 0.1959967359906118 No Hit ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC 3464 0.19320793781203166 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 3439 0.1918135387227416 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 3424 0.19097689926916755 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 3321 0.1852319750212925 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 3118 0.17390945441625713 No Hit ACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTTGATTCATAAA 3111 0.1735190226712559 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 3071 0.1712879841283918 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 3000 0.16732789071480803 No Hit TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA 2922 0.162977365556223 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 2921 0.1629215895926514 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 2859 0.15946347985121204 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 2857 0.15935192792406883 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 2853 0.15912882406978243 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 2845 0.15868261636120962 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 2834 0.15806908076192197 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 2827 0.15767864901692075 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2814 0.15695356149048992 No Hit TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC 2800 0.15617269800048747 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 2735 0.1525472603683333 No Hit GATGAAAGACTAATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACC 2713 0.15132018916975803 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 2634 0.14691388804760144 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 2586 0.14423664179616452 No Hit AACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAACAAG 2518 0.14044387627329552 No Hit TTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGT 2498 0.13932835700186347 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 2476 0.13810128580328823 No Hit GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT 2461 0.1372646463497142 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 2458 0.13709731845899936 No Hit CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC 2426 0.13531248762470807 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 2423 0.13514515973399327 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 2421 0.13503360780685006 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 2398 0.1337507606447032 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 2390 0.1333045529361304 No Hit TTTCACCTCTACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTT 2379 0.13269101733684277 No Hit CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC 2308 0.12873092392325897 No Hit GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG 2278 0.1270576450161109 No Hit GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCT 2275 0.12689031712539606 No Hit ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG 2271 0.12666721327110966 No Hit GTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACCGC 2236 0.12471505454610357 No Hit ACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAG 2188 0.12203780829466665 No Hit TCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAA 2133 0.11897013029822849 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 2127 0.11863547451679889 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 2108 0.11757573120893844 No Hit CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT 2105 0.11740840331822362 No Hit CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC 2094 0.11679486771893599 No Hit GATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGT 2086 0.11634866001036318 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 2083 0.11618133211964837 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 2080 0.11601400422893354 No Hit AATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACCTTTTGCATAAT 2078 0.11590245230179036 No Hit ACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTC 2069 0.11540046862964592 No Hit CTACTATACAATCAAAGATATCCTAGGTATCCTAATCATATTCTTAATTC 2065 0.11517736477535952 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 2028 0.11311365412321021 No Hit GGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTA 2024 0.1128905502689238 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 2017 0.1125001185239226 No Hit ATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTC 1976 0.11021330401748688 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 1973 0.11004597612677208 No Hit CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG 1971 0.10993442419962886 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1963 0.10948821649105604 No Hit ATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAA 1931 0.10770338565676475 No Hit GTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTAT 1927 0.10748028180247834 No Hit CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA 1917 0.10692252216676233 No Hit GTACAAGGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCT 1892 0.10552812307747227 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1891 0.10547234711390065 No Hit ATATAGGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATT 1862 0.10385484417032417 No Hit GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA 1850 0.10318553260746494 No Hit GCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGG 1819 0.10145647773674527 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 5.577596357160267E-5 0.0 6 0.0 0.0 0.0 5.577596357160267E-5 0.0 7 0.0 0.0 0.0 5.577596357160267E-5 0.0 8 0.0 0.0 0.0 5.577596357160267E-5 0.0 9 0.0 0.0 0.0 1.1155192714320534E-4 0.0 10-11 0.0 0.0 0.0 6.971995446450334E-4 0.0 12-13 0.0 0.0 0.0 0.0015617269800048748 0.0 14-15 0.0 0.0 0.0 0.002482030378936319 0.0 16-17 0.0 0.0 0.0 0.0065257877378775125 0.0 18-19 0.0 0.0 0.0 0.009677129679673064 0.0 20-21 0.0 0.0 0.0 0.017011668889338815 0.0 22-23 0.0 0.0 0.0 0.03728623164761639 0.0 24-25 0.0 0.0 0.0 0.05990338487590127 0.0 26-27 0.0 0.0 0.0 0.07186732906201004 0.0 28-29 0.0 0.0 0.0 0.08240898617704295 0.0 30-31 0.0 0.0 0.0 0.10170746957281747 0.0 32-33 0.0 0.0 0.0 0.13695787855007036 0.0 34-35 0.0 0.0 0.0 0.19343104166631808 0.0 36-37 0.0 0.0 0.0 0.2782941702405115 0.0 38-39 0.0 0.0 0.0 0.4035390964405453 0.0 40-41 0.0 0.0 0.0 0.5566162284628089 0.0 42-43 0.0 0.0 0.0 0.6930163473771631 0.0 44-45 0.0 0.0 0.0 0.8496631410680093 0.0 46-47 0.0 0.0 0.0 1.0381858979400262 0.0 48-49 0.0 0.0 0.0 1.2397602302877984 0.0 50-51 0.0 0.0 0.0 1.4661548664249335 0.0 52-53 0.0 0.0 0.0 1.671466188332003 0.0 54-55 0.0 0.0 0.0 1.8715624576451275 0.0 56-57 0.0 0.0 0.0 2.1140205712908844 0.0 58-59 0.0 0.0 0.0 2.38704391297388 0.0 60-61 0.0 0.0 0.0 2.6885966600237494 0.0 62-63 0.0 0.0 0.0 2.964353023921753 0.0 64-65 0.0 0.0 0.0 3.2464120717033476 0.0 66-67 0.0 0.0 0.0 3.560932730283615 0.0 68-69 0.0 0.0 0.0 3.907831335717198 0.0 70-71 0.0 0.0 0.0 4.345839977644994 0.0 72-73 0.0 0.0 0.0 4.753283391535551 0.0 74-75 0.0 0.0 0.0 5.125671612321357 0.0 76-77 0.0 0.0 0.0 5.554783988059482 0.0 78-79 0.0 0.0 0.0 6.033509083394547 0.0 80-81 0.0 0.0 0.0 6.585495906880913 0.0 82-83 0.0 0.0 0.0 7.115144456956852 0.0 84-85 0.0 0.0 0.0 7.608148198966248 0.0 86-87 0.0 0.0 0.0 8.13425497535539 0.0 88-89 0.0 0.0 0.0 8.716277155225065 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTGA 15 6.620962E-4 94.99125 8 TGGGATA 220 0.0 51.813404 6 ATGTATT 140 0.0 47.523453 1 ACGTCAG 20 5.388833E-4 47.501583 30-31 TTTAGAC 50 2.8277762E-5 47.499596 3 ACGTGGG 40 5.389219E-4 47.499596 3 GACCGAT 20 5.390686E-4 47.498272 18-19 ACGACCG 20 5.391427E-4 47.49695 16-17 ACCGCTA 20 5.3921685E-4 47.49562 38-39 GGGATGC 235 0.0 44.463985 7 TAACTTT 3740 0.0 43.9398 6 TGGGTAT 260 0.0 43.84211 6 GACATAT 470 0.0 43.478905 1 GACATTC 55 4.96713E-5 43.177837 7 GGATGCC 245 0.0 42.64913 8 GTCGGGA 115 5.4569682E-11 41.3063 2 AACTTTA 4060 0.0 40.593548 7 GAGAGCG 60 8.297118E-5 39.57969 9 GGGCATT 145 0.0 39.30672 7 ACTTTAT 4215 0.0 39.100784 8 >>END_MODULE