FastQCFastQC Report
Fri 10 Feb 2017
SRR2031642_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031642_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072700
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG3150.028.7501851
GGTATCA16150.028.3332461
GTACATG27150.026.3341351
TACATGG27850.025.9719282
ACCGTGC2251.0186341E-1025.3440118
GTATCAA21550.024.7724191
ACATGGG28150.023.813
TCAACGC23750.022.0175044
GTGTAGC8050.021.908041
ATCAACG24750.021.126923
CAACGCA24900.021.0006315
TATCAAC25400.020.9831222
TACCTGG8450.020.836792
CATGGGG17200.020.4523054
TAGGACA6350.020.212924
AACGCAG26150.020.1719636
GTACCTG9550.019.9643061
GTATAGC5300.019.7853811
GCACCGT2803.4689947E-818.669456
ACGCAGA28100.018.6021447
Produced by FastQC (version 0.11.5)