##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031642_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1072700 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.911343339237437 33.0 31.0 34.0 30.0 34.0 2 32.15988347161368 34.0 31.0 34.0 30.0 34.0 3 32.315141232404216 34.0 31.0 34.0 30.0 34.0 4 35.39533700009322 37.0 35.0 37.0 33.0 37.0 5 35.08998321991237 37.0 35.0 37.0 32.0 37.0 6 35.30395171063671 37.0 35.0 37.0 32.0 37.0 7 35.45405332338958 37.0 35.0 37.0 33.0 37.0 8 35.53810851123334 37.0 35.0 37.0 33.0 37.0 9 37.47185979304559 39.0 38.0 39.0 35.0 39.0 10-11 37.420235387340355 39.0 38.0 39.0 35.0 39.0 12-13 37.44607998508437 39.0 38.0 39.0 35.0 39.0 14-15 38.86972685746248 41.0 39.0 41.0 35.5 41.0 16-17 38.824607066281345 41.0 39.0 41.0 35.0 41.0 18-19 38.73748904633169 41.0 38.5 41.0 34.0 41.0 20-21 38.72701407662907 41.0 39.0 41.0 34.0 41.0 22-23 38.66956558217582 40.0 39.0 41.0 34.0 41.0 24-25 38.594852242006155 40.5 38.0 41.0 34.0 41.0 26-27 38.5048135545819 40.0 38.0 41.0 34.0 41.0 28-29 38.3900247040179 40.0 38.0 41.0 34.0 41.0 30-31 38.26702479724061 40.0 38.0 41.0 34.0 41.0 32-33 38.12678008762935 40.0 38.0 41.0 33.0 41.0 34-35 37.996530250769084 40.0 38.0 41.0 33.0 41.0 36-37 37.876302787358995 40.0 38.0 41.0 33.0 41.0 38-39 37.71276871445884 40.0 38.0 41.0 32.0 41.0 40-41 37.55795842267176 40.0 37.0 41.0 31.5 41.0 42-43 37.383338771324695 40.0 37.0 41.0 31.0 41.0 44-45 37.18915540225599 40.0 37.0 41.0 31.0 41.0 46-47 37.0170154749697 40.0 37.0 41.0 30.0 41.0 48-49 37.18019903048383 40.0 37.0 41.0 30.5 41.0 50-51 36.95207187470868 39.5 36.5 40.5 30.5 41.0 52-53 37.07319194555794 39.5 36.5 40.5 31.0 41.0 54-55 37.34464808427333 40.0 37.0 41.0 31.0 41.0 56-57 37.23538500978839 40.0 36.5 41.0 31.0 41.0 58-59 37.03882073273049 40.0 36.0 41.0 31.0 41.0 60-61 36.74956604828937 39.0 35.5 41.0 30.0 41.0 62-63 36.402920201361056 39.0 35.0 41.0 29.0 41.0 64-65 36.12533140673068 39.0 35.0 41.0 29.0 41.0 66-67 35.888644541810386 38.0 35.0 40.5 29.0 41.0 68-69 35.61785121655635 37.0 35.0 40.0 29.0 41.0 70-71 35.290116994499854 37.0 35.0 39.5 29.0 41.0 72-73 34.90584692831173 36.0 34.0 39.0 28.0 41.0 74-75 34.47538221310711 36.0 34.0 39.0 28.0 40.5 76-77 34.07053090332805 35.0 34.0 37.5 27.0 39.5 78-79 33.71496690593828 35.0 34.0 37.0 26.5 39.0 80-81 33.38775333271185 35.0 34.0 36.5 26.0 39.0 82-83 33.073254870886544 35.0 33.5 36.0 26.0 37.5 84-85 32.821011932506764 35.0 33.0 36.0 26.0 37.0 86-87 32.59734035611075 35.0 33.0 35.5 25.5 37.0 88-89 32.36308427332898 35.0 33.0 35.0 25.0 36.0 90-91 32.188362077001955 35.0 33.0 35.0 25.0 36.0 92-93 32.02347907150182 35.0 33.0 35.0 25.0 36.0 94-95 31.87244336720425 35.0 32.0 35.0 24.0 35.5 96-97 31.771650041950217 35.0 32.0 35.0 24.0 35.0 98-99 31.664162860072715 35.0 32.0 35.0 24.0 35.0 100-101 31.215224666728815 34.5 31.5 35.0 21.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23297391349208496 1101 2 0.3205478666212329 1101 3 0.33240936934717524 1101 4 0.4272933456237382 1101 5 0.2789172992570528 1101 6 0.30315634991082163 1101 7 0.27983604546671614 1101 8 0.36765037470414086 1101 9 0.26780639066845424 1101 10-11 0.38883979565196825 1101 12-13 0.35899214256424017 1101 14-15 0.33658935058274153 1101 16-17 0.2920824978694512 1101 18-19 0.45061599261667595 1101 20-21 0.21626196213344429 1101 22-23 0.3482999800883917 1101 24-25 0.3963549411599203 1101 26-27 0.2200845709359882 1101 28-29 0.37062233298010483 1101 30-31 0.263620692724885 1101 32-33 0.2996375331244323 1101 34-35 0.36765157897205114 1101 36-37 0.4108217244065884 1101 38-39 0.3491231557696537 1101 40-41 0.16979707309180725 1101 42-43 0.09978938993935316 1101 44-45 0.28968845868393345 1101 46-47 0.29569546543337566 1101 48-49 0.4365285557519556 1101 50-51 0.2810424571536885 1101 52-53 0.3946996591392704 1101 54-55 0.2980169883842052 1101 56-57 0.49361932700850275 1101 58-59 0.541397970049438 1101 60-61 0.1938041948928131 1101 62-63 -0.03299318755946956 1101 64-65 0.16545495640579588 1101 66-67 0.22622823334356212 1101 68-69 0.200112823023737 1101 70-71 0.24931610772856772 1101 72-73 0.32643563375566487 1101 74-75 0.3003478172002616 1101 76-77 0.27944014304338793 1101 78-79 -0.03426607470969145 1101 80-81 0.07326278438391398 1101 82-83 0.32489384169073077 1101 84-85 0.2595463719065165 1101 86-87 0.2712245974078016 1101 88-89 0.37486248722410664 1101 90-91 0.02857007772339415 1101 92-93 0.12006117234799518 1101 94-95 0.059114580188108334 1101 96-97 0.08221363042552454 1101 98-99 -0.05754627025325121 1101 100-101 -0.008165395932291375 1102 1 -0.06256421222473563 1102 2 0.059462859160113624 1102 3 0.16317632335952936 1102 4 0.2140150149418787 1102 5 0.16405178443963564 1102 6 -0.009008362065202391 1102 7 0.00806745124076258 1102 8 0.22177372369966264 1102 9 0.4130392886669014 1102 10-11 0.41784197849262483 1102 12-13 0.3296717829504914 1102 14-15 0.19406835461544603 1102 16-17 0.18822182969822876 1102 18-19 0.45192199727833327 1102 20-21 0.27011276435820974 1102 22-23 0.1600922525858337 1102 24-25 0.42663353082287614 1102 26-27 0.25738709785152025 1102 28-29 0.17782200740386855 1102 30-31 0.23241963706861668 1102 32-33 0.32266465214474493 1102 34-35 -0.03764335116471074 1102 36-37 0.14417417301117297 1102 38-39 -0.03344433347030673 1102 40-41 0.22253857296231416 1102 42-43 -0.036578338549794864 1102 44-45 -0.4558287018966709 1102 46-47 -0.6429408141182122 1102 48-49 -0.14295459197825977 1102 50-51 -0.19039007827122845 1102 52-53 -0.14438860408417753 1102 54-55 -0.2646971334849013 1102 56-57 -0.197186227519353 1102 58-59 -0.37831480758446645 1102 60-61 -0.6616337914675157 1102 62-63 -0.8722393059261719 1102 64-65 -0.2908481501340958 1102 66-67 -0.21142268480607385 1102 68-69 -0.08959576158292037 1102 70-71 0.0067463986506624 1102 72-73 -0.19453443542928284 1102 74-75 -0.30812394769423435 1102 76-77 -0.2730158610016531 1102 78-79 -0.16369372649111114 1102 80-81 -0.1733852603088195 1102 82-83 -0.19197939322979352 1102 84-85 -0.24909311407767376 1102 86-87 -0.17904363301138204 1102 88-89 -0.32689920083933544 1102 90-91 -0.18510905703309533 1102 92-93 -0.2665772530469539 1102 94-95 -0.21857666501766104 1102 96-97 -0.3521950910711418 1102 98-99 -0.21532803551772517 1102 100-101 -0.21796104751638978 1103 1 0.15012045410231778 1103 2 -0.017428681969839488 1103 3 -0.07417175902166662 1103 4 0.04423115641629494 1103 5 0.024469512407030436 1103 6 0.05447318949582325 1103 7 0.24333902293617626 1103 8 0.14912253382993157 1103 9 0.28156414129771434 1103 10-11 0.2755670348931787 1103 12-13 0.09046430169003372 1103 14-15 0.3150748315821872 1103 16-17 0.11056736495766728 1103 18-19 0.15648498396053157 1103 20-21 -0.06407446121973948 1103 22-23 -0.05741860300448565 1103 24-25 0.10669320624679557 1103 26-27 0.12537341616896924 1103 28-29 -0.12644624090410872 1103 30-31 0.05148059818255035 1103 32-33 0.17875227313302133 1103 34-35 0.3025708496455124 1103 36-37 0.11863988390071967 1103 38-39 -0.06682011883284389 1103 40-41 0.004188380108807621 1103 42-43 -0.2530315321553118 1103 44-45 -0.12120846562081056 1103 46-47 -0.02918635665515268 1103 48-49 -0.15859391953812008 1103 50-51 -0.17397933669492005 1103 52-53 -0.0035230678544095895 1103 54-55 -0.25031317307444567 1103 56-57 -0.14145035191592115 1103 58-59 0.06972997324177044 1103 60-61 0.08901875686715499 1103 62-63 0.20058775663599704 1103 64-65 0.14221227729719743 1103 66-67 -0.11724589029815746 1103 68-69 -0.1844166140196748 1103 70-71 -0.14634047033025155 1103 72-73 0.1246890563107641 1103 74-75 -0.02928567779636637 1103 76-77 -0.42219593038039704 1103 78-79 -0.14754497458076798 1103 80-81 -0.039319326242889474 1103 82-83 -0.4894225681564066 1103 84-85 -0.3039811620120787 1103 86-87 -0.2881805226118459 1103 88-89 -0.28537141295393553 1103 90-91 -0.32751378255040464 1103 92-93 -0.3249354511402487 1103 94-95 -0.08642228518116468 1103 96-97 -0.23158402443358383 1103 98-99 -0.22015907644147958 1103 100-101 -0.14059525657444283 1104 1 -0.03825773117989684 1104 2 0.10146697345832223 1104 3 0.3011625042382917 1104 4 0.10211908891722743 1104 5 -0.016611697404826486 1104 6 0.1329930184800432 1104 7 0.1659929428037188 1104 8 0.2124618776646301 1104 9 0.3352605996381328 1104 10-11 0.28197445616127226 1104 12-13 0.25530003355362396 1104 14-15 0.41795930520969904 1104 16-17 0.21597454807382377 1104 18-19 0.45878371721902056 1104 20-21 0.2951505243210377 1104 22-23 0.39270387513656146 1104 24-25 0.3618299168014616 1104 26-27 0.522778127041164 1104 28-29 0.4827243099073044 1104 30-31 0.31195134518580403 1104 32-33 0.18037964472944168 1104 34-35 0.15739796267695993 1104 36-37 0.3333174148321234 1104 38-39 0.3342198113092536 1104 40-41 0.4352535997189264 1104 42-43 0.3595100026772471 1104 44-45 0.42943397759129454 1104 46-47 0.7075604878316355 1104 48-49 0.7201510197331302 1104 50-51 0.5435696262100791 1104 52-53 0.34782532575405156 1104 54-55 0.4830150570030014 1104 56-57 0.24820534842088904 1104 58-59 0.3030338210967045 1104 60-61 0.5340345769603729 1104 62-63 0.6978524114857834 1104 64-65 0.5535415053446897 1104 66-67 0.49075885005952813 1104 68-69 0.4784951903782044 1104 70-71 0.4689573897044568 1104 72-73 0.3267717517371125 1104 74-75 0.33385180391697133 1104 76-77 0.385138582372079 1104 78-79 0.521757240232283 1104 80-81 0.6246023557902518 1104 82-83 0.6827839662792243 1104 84-85 0.5774724720731328 1104 86-87 0.494764963274811 1104 88-89 0.5577210467019924 1104 90-91 0.5188220797297234 1104 92-93 0.5384600285090997 1104 94-95 0.5155333958766875 1104 96-97 0.6060434152062335 1104 98-99 0.5211364701172307 1104 100-101 0.24021253212808347 1105 1 -0.12411667240753843 1105 2 0.05630667358369834 1105 3 0.14169419320792542 1105 4 -0.001688832685509567 1105 5 -0.10744402859742053 1105 6 -0.17439339846167456 1105 7 -0.046162384747049146 1105 8 0.056231334120546705 1105 9 0.1873606175487339 1105 10-11 0.1675443757341455 1105 12-13 0.08249045735254867 1105 14-15 0.09339310421864155 1105 16-17 0.1633600268412394 1105 18-19 0.07574526912991075 1105 20-21 -0.053968657932117026 1105 22-23 0.12781888677844933 1105 24-25 0.0937220864574897 1105 26-27 0.18140261424361626 1105 28-29 0.0554525421310359 1105 30-31 0.1235403566059432 1105 32-33 0.16679264185521703 1105 34-35 -0.05678456766445095 1105 36-37 0.09700356567349644 1105 38-39 0.18554796762615666 1105 40-41 0.16770440953393262 1105 42-43 0.13612590132030533 1105 44-45 0.311803976482544 1105 46-47 0.3885742917873145 1105 48-49 0.22471822286492937 1105 50-51 0.19594619232151445 1105 52-53 0.15303741711516494 1105 54-55 0.3242860912975445 1105 56-57 0.10049406730122712 1105 58-59 0.19185216922760873 1105 60-61 0.26987880590901625 1105 62-63 0.23155792283343146 1105 64-65 -0.031997603730872015 1105 66-67 0.10222781781191514 1105 68-69 -0.032723988463409626 1105 70-71 0.009029160039382589 1105 72-73 0.16692977014238863 1105 74-75 -0.03305804179599647 1105 76-77 0.18211339665620585 1105 78-79 -0.005348534657052539 1105 80-81 0.06266845869944859 1105 82-83 0.003334674600829146 1105 84-85 0.03711978249796033 1105 86-87 0.1157643961057957 1105 88-89 0.13100312160241856 1105 90-91 0.2917828299280245 1105 92-93 -0.15722021183410106 1105 94-95 -0.11264946709016499 1105 96-97 -0.014417100448479658 1105 98-99 -0.05354273823423128 1105 100-101 0.14980184632266003 1106 1 0.20942639988407663 1106 2 0.254939325146438 1106 3 0.1877116685083564 1106 4 0.192333301063357 1106 5 0.3108617973185659 1106 6 0.09666752033773207 1106 7 0.2668021343558564 1106 8 0.3113476729553639 1106 9 0.2684847231837395 1106 10-11 0.331141273093273 1106 12-13 0.26251175078584765 1106 14-15 0.40110208172592365 1106 16-17 0.36548855433097316 1106 18-19 0.3698139057790897 1106 20-21 0.23807669463329262 1106 22-23 0.39219195425661013 1106 24-25 0.3186400945016743 1106 26-27 0.30991123767618234 1106 28-29 0.3197027236555172 1106 30-31 0.4030676587034776 1106 32-33 0.22499724237898278 1106 34-35 0.12873294026959314 1106 36-37 0.3289536076892787 1106 38-39 0.3803512685162289 1106 40-41 0.31625568102112567 1106 42-43 0.043987440974227354 1106 44-45 0.2883648830443448 1106 46-47 0.45016666939086747 1106 48-49 0.3257518925964362 1106 50-51 0.10771305778128237 1106 52-53 -0.06490260638103251 1106 54-55 0.2906005528837028 1106 56-57 0.27319931233887473 1106 58-59 0.3928155747084219 1106 60-61 0.5798614285094246 1106 62-63 0.4549253243003122 1106 64-65 0.5597426245412649 1106 66-67 0.3849481151041374 1106 68-69 0.20095053610562275 1106 70-71 0.4020898259282788 1106 72-73 0.46990075478011306 1106 74-75 0.4803712003698948 1106 76-77 0.39871085286597463 1106 78-79 0.4101966914531019 1106 80-81 0.5233077308066783 1106 82-83 0.5195107492665372 1106 84-85 0.3634564349418028 1106 86-87 0.34164764527159974 1106 88-89 0.43952742956583535 1106 90-91 0.5850172698128233 1106 92-93 0.44854176266272816 1106 94-95 0.39614988105338966 1106 96-97 0.4808255304734992 1106 98-99 0.3831669164205458 1106 100-101 0.5657524431098828 1107 1 0.026262511278854817 1107 2 -0.0511788398579327 1107 3 0.06016574255721707 1107 4 0.08244007393555819 1107 5 0.024027425758049503 1107 6 0.10833197438908826 1107 7 0.12238832612341355 1107 8 0.13998818159213045 1107 9 0.20071912424580063 1107 10-11 0.24473675406289175 1107 12-13 0.09579355098479425 1107 14-15 0.07265872278677676 1107 16-17 0.08696520083825732 1107 18-19 -0.034902747082369956 1107 20-21 -0.11284503930065881 1107 22-23 -0.0022672409565771545 1107 24-25 -0.03986693858921342 1107 26-27 -0.02859904814832248 1107 28-29 0.05066524913841164 1107 30-31 0.14493771577760128 1107 32-33 0.16880532353090416 1107 34-35 0.2633861837367064 1107 36-37 0.11912723180186191 1107 38-39 0.3189667954023534 1107 40-41 0.24375092484377348 1107 42-43 0.17673774071741377 1107 44-45 0.10178846082438753 1107 46-47 0.16340132386356743 1107 48-49 0.02563095520399372 1107 50-51 -0.014896561232838224 1107 52-53 0.05960901079491521 1107 54-55 0.2255657372872193 1107 56-57 0.12065650674266237 1107 58-59 0.16633643191719472 1107 60-61 0.2842347477727927 1107 62-63 0.22704769516489876 1107 64-65 0.33480522075220875 1107 66-67 0.08927699294886082 1107 68-69 -0.010956549180299646 1107 70-71 -0.014481811747451445 1107 72-73 -0.0031824017331061327 1107 74-75 0.15296559583422464 1107 76-77 0.06533827870553921 1107 78-79 0.032177984452538055 1107 80-81 0.1326492224497997 1107 82-83 0.30397034161580905 1107 84-85 0.20832042528950723 1107 86-87 0.1699959752198268 1107 88-89 0.1819752250823612 1107 90-91 0.07777757360302218 1107 92-93 0.12503612900374605 1107 94-95 0.1415639048739905 1107 96-97 0.33442793295768425 1107 98-99 0.21530364091778864 1107 100-101 0.09540006993812966 1108 1 0.10961194300557153 1108 2 0.13503127637247303 1108 3 0.07638487388791049 1108 4 0.20630111260389583 1108 5 0.05287574669561934 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-1.238200089193839 2316 76-77 -1.1478059486540673 2316 78-79 -1.2624260303196095 2316 80-81 -1.2364025665541192 2316 82-83 -1.6099254663116795 2316 84-85 -1.9089043050842065 2316 86-87 -1.4084572737012415 2316 88-89 -1.3296660573614432 2316 90-91 -1.3373571746737092 2316 92-93 -1.5900951597536839 2316 94-95 -1.590061723244979 2316 96-97 -1.275385858416005 2316 98-99 -0.8109097854766141 2316 100-101 -1.410415165784869 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 4.0 7 67.0 8 259.0 9 322.0 10 312.0 11 458.0 12 588.0 13 869.0 14 1165.0 15 1432.0 16 1841.0 17 2476.0 18 3146.0 19 3917.0 20 4702.0 21 5747.0 22 6490.0 23 6922.0 24 7478.0 25 8160.0 26 9063.0 27 10288.0 28 11992.0 29 14454.0 30 17343.0 31 20985.0 32 26505.0 33 34218.0 34 47960.0 35 72173.0 36 123366.0 37 231885.0 38 305271.0 39 90717.0 40 125.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.07575438290874 18.00057505336102 12.358308818639971 24.56536174509027 2 15.495051635514457 18.85823670191367 41.12082467127853 24.52588699129335 3 19.374106276403083 23.438231148794998 29.25940143518817 27.928261139613753 4 12.348096623954183 15.464036960390906 37.81741230549314 34.37045411016177 5 13.451559154807171 37.650348238807815 35.28084784678801 13.617244759597009 6 33.18239557647762 36.32920496151834 17.183740566390195 13.304658895613839 7 28.970813070469468 31.6996336106859 20.935745046085856 18.393808272758772 8 25.99213983913568 36.85785960049163 19.18267775955529 17.9673228008174 9 25.972463749166458 15.777401296680596 19.523896691428497 38.72623826272445 10-11 25.128970579856208 26.13280677157426 28.38935991548609 20.34886273308344 12-13 25.566820440044957 23.065446379414446 28.47179708451652 22.895936096024073 14-15 22.479728827237583 25.033462263067097 25.61448670803253 26.87232220166279 16-17 22.71863818511763 28.28112389847034 26.293775453557316 22.706462462854716 18-19 22.680867591853026 27.731484266189945 27.49913855367475 22.088509588282278 20-21 23.269865670549553 26.933616482170898 27.62519011632812 22.17132773095143 22-23 22.906497622820922 26.81276218886921 27.360492215903793 22.920247972406077 24-25 22.731984711475718 26.778829122774304 27.650601286473385 22.838584879276592 26-27 22.77337559429477 27.125664211801997 27.362216836021254 22.73874335788198 28-29 22.759858301482243 27.03854759019297 27.45921506478978 22.742379043535006 30-31 23.000559336254312 26.88836580591032 27.441223081942763 22.669851775892607 32-33 22.70308567166962 27.073319660669338 27.427472732357604 22.79612193530344 34-35 22.96294397315186 27.05821758180293 27.466859326932042 22.511979118113175 36-37 22.941269693297286 26.969562785494546 27.3499114384264 22.73925608278177 38-39 22.896569404306888 27.11279947795283 27.400391535378017 22.590239582362265 40-41 23.16313974084087 26.977999440663748 27.308660389670926 22.550200428824464 42-43 22.839144215530904 26.962850750442808 27.44346042696001 22.754544607066283 44-45 22.972672729020978 27.087960149402402 27.334085759815384 22.605281361761236 46-47 22.939003480164093 27.03631168930649 27.113472219173268 22.911212611356145 48-49 22.85365203959912 27.09650151172126 27.236267501654936 22.81357894702468 50-51 22.874967119193624 27.218684879679568 27.07820688136978 22.828141119757028 52-53 22.899317426838177 27.289306726941813 27.05851733205967 22.752858514160337 54-55 22.789137065735222 27.192927216437234 27.219049458266685 22.79888625956086 56-57 22.799112127706533 27.192558820139602 27.17501415005639 22.833314902097474 58-59 22.812979364244047 27.22364359746653 27.23498038688777 22.728396651401656 60-61 22.985194451186498 27.1466645506992 27.147318011239523 22.72082298687478 62-63 22.761437229040798 27.257882375147936 27.22330799280292 22.757372403008343 64-65 22.76832012934217 27.196674591861786 27.222345129645554 22.812660149150492 66-67 22.785945276591494 27.09971907163463 27.171572531635352 22.94276312013852 68-69 22.830792431138082 27.029883324110372 27.194170218206214 22.945154026545328 70-71 22.79838623899263 26.937380922612995 27.16210012857092 23.102132709823454 72-73 22.684595438853936 27.062849743760292 27.217568430484572 23.0349863869012 74-75 22.678016083163087 27.112064519351094 27.14090947491446 23.069009922571365 76-77 22.650853965280877 27.06372802029058 27.101142279280392 23.184275735148148 78-79 22.68884775402722 26.913167791963392 27.100141607228036 23.297842846781354 80-81 22.756160403513952 27.050260570105277 27.024742380581166 23.168836645799598 82-83 22.722830267810117 27.057139698195208 27.113540909071826 23.10648912492285 84-85 22.71795015349588 27.079705885097372 27.059177560675195 23.143166400731555 86-87 22.731973164805652 27.09504776573172 27.151906734499402 23.02107233496323 88-89 22.67065459836797 26.993510980108393 27.12931899581379 23.206515425709842 90-91 22.70093984322103 26.900906525706514 27.098898422177705 23.29925520889475 92-93 22.723690648261233 26.86323282800554 27.19946441976941 23.213612103963815 94-95 22.71464407807997 26.887838799631304 27.18961111694494 23.207906005343787 96-97 22.688999965476476 26.857482227383127 27.170153387084284 23.283364420056117 98-99 22.631715945858197 26.86070464952251 27.100756447718528 23.406822956900765 100-101 22.71730451362071 26.755804799056754 27.088253840327447 23.438636846995088 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 21.0 1 16.5 2 23.5 3 41.0 4 35.5 5 37.5 6 56.0 7 107.5 8 186.0 9 218.0 10 231.0 11 239.5 12 243.0 13 262.0 14 289.0 15 339.5 16 380.5 17 432.0 18 484.0 19 542.0 20 647.0 21 795.0 22 959.0 23 1214.0 24 1604.0 25 2057.5 26 2757.5 27 3739.5 28 5060.5 29 6928.0 30 9021.0 31 11282.5 32 14054.5 33 17138.5 34 20179.5 35 22790.0 36 25726.5 37 28297.5 38 30522.5 39 32920.5 40 35092.0 41 36722.0 42 37739.5 43 39251.0 44 41614.5 45 43727.5 46 44573.0 47 45052.5 48 45418.0 49 45484.5 50 44806.5 51 43450.0 52 41871.0 53 39791.0 54 37409.0 55 35044.0 56 31635.5 57 27468.0 58 23978.0 59 20551.0 60 17019.5 61 13749.0 62 10695.0 63 7991.5 64 5746.0 65 4058.5 66 2906.0 67 2108.0 68 1528.0 69 1127.5 70 831.0 71 645.5 72 492.5 73 348.5 74 234.5 75 169.0 76 128.5 77 87.5 78 52.5 79 38.5 80 39.5 81 30.5 82 21.5 83 16.5 84 16.0 85 12.5 86 7.0 87 7.5 88 7.0 89 6.0 90 6.5 91 4.5 92 3.0 93 3.5 94 3.5 95 3.0 96 3.5 97 2.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4635965321152233 2 0.36002610235853455 3 0.19008110375687517 4 0.22047170690780274 5 0.24247226624405704 6 0.1920387806469656 7 0.18467418663186352 8 0.1849538547590193 9 0.18327584599608465 10-11 0.1964202479724061 12-13 0.18350890276871445 14-15 0.1949286846275753 16-17 0.08343432460147292 18-19 0.035005127248997854 20-21 1.398340635778876E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.13974084086883565 46-47 0.2055094621049688 48-49 0.0846928311736739 50-51 0.060221870047543584 52-53 0.06777290948074952 54-55 0.0761163419408968 56-57 0.10715950405518786 58-59 0.09023958236226344 60-61 0.13796960939684907 62-63 0.23711196047357133 64-65 0.1335881420714086 66-67 0.10016780087629346 68-69 0.10245175724806564 70-71 0.08557844690966719 72-73 0.07033653397967744 74-75 0.1356856530250769 76-77 0.20998415213946117 78-79 0.13302880581709703 80-81 0.08534539013703739 82-83 0.08525216742798546 84-85 0.0941549361424443 86-87 0.06987042043441782 88-89 0.09009974829868557 90-91 0.11107485783536869 92-93 0.15959727789689568 94-95 0.12701594108324787 96-97 0.09014635965321152 98-99 0.07905285727603244 100-101 0.06115409713806283 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1072700.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.65818354968647 #Duplication Level Percentage of deduplicated Percentage of total 1 77.34130145302713 53.101132711335694 2 13.838920276217376 19.003102569080205 3 4.421181435609015 9.106508595375303 4 1.8737702898469772 5.145986579610518 5 0.94405720737494 3.240862651267652 6 0.5360582836951305 2.2082872821162103 7 0.3072670893785037 1.4767480154929058 8 0.20119661061485378 1.1051035056935543 9 0.12807322945618574 0.7913947766223519 >10 0.39861570499767274 4.0503912609181025 >50 0.007376550362599037 0.3263162421695004 >100 0.00163640332048178 0.17405255322638136 >500 4.0909957435811603E-4 0.17386492839488066 >1k 1.363665247860387E-4 0.09624832869678908 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.322270905192505E-5 0.0 10-11 0.0 0.0 0.0 1.864454181038501E-4 0.0 12-13 0.0 0.0 0.0 1.864454181038501E-4 0.0 14-15 0.0 0.0 0.0 2.796681271557752E-4 0.0 16-17 0.0 0.0 0.0 6.059476088375129E-4 0.0 18-19 0.0 0.0 0.0 7.92393026941363E-4 0.0 20-21 0.0 0.0 0.0 0.0011186725086231007 0.0 22-23 0.0 0.0 0.0 0.001957676890090426 0.0 24-25 0.0 0.0 0.0 0.0030763493987135265 0.0 26-27 0.0 0.0 0.0 0.004428078679966439 0.0 28-29 0.0 0.0 0.0 0.006851869115316491 0.0 30-31 0.0 0.0 0.0 0.010207886641185793 0.0 32-33 0.0 0.0 0.0 0.018504707746807122 0.0 34-35 0.0 0.0 0.0 0.03150927565955067 0.0 36-37 0.0 0.0 0.0 0.05276405332338958 0.0 38-39 0.0 0.0 0.0 0.0829682110562133 0.0 40-41 0.0 0.0 0.0 0.11997762654982755 0.0 42-43 0.0 0.0 0.0 0.1548429197352475 0.0 44-45 0.0 0.0 0.0 0.19581430036356856 0.0 46-47 0.0 0.0 0.0 0.24657406544234176 0.0 48-49 0.0 0.0 0.0 0.302228022746341 0.0 50-51 0.0 0.0 0.0 0.36179733383052115 0.0 52-53 0.0 0.0 0.0 0.42076069730586374 4.661135452596253E-5 54-55 0.0 0.0 0.0 0.4849911438426401 9.322270905192505E-5 56-57 0.0 0.0 0.0 0.5615735993287965 9.322270905192505E-5 58-59 0.0 0.0 0.0 0.6448680898666915 9.322270905192505E-5 60-61 0.0 0.0 0.0 0.7339889997203319 9.322270905192505E-5 62-63 0.0 0.0 0.0 0.8280973245082501 9.322270905192505E-5 64-65 0.0 0.0 0.0 0.9245362170224667 9.322270905192505E-5 66-67 0.0 0.0 0.0 1.0390603150927564 9.322270905192505E-5 68-69 0.0 0.0 0.0 1.1694322737018736 9.322270905192505E-5 70-71 0.0 0.0 0.0 1.3126689661601567 9.322270905192505E-5 72-73 0.0 0.0 0.0 1.463782977533327 1.864454181038501E-4 74-75 0.0 0.0 0.0 1.6135452596252446 1.864454181038501E-4 76-77 0.0 0.0 0.0 1.7792020136105156 1.864454181038501E-4 78-79 0.0 0.0 0.0 1.9719399645753706 1.864454181038501E-4 80-81 0.0 0.0 0.0 2.185419968304279 1.864454181038501E-4 82-83 0.0 0.0 0.0 2.4037941642584135 1.864454181038501E-4 84-85 0.0 0.0 0.0 2.62640999347441 1.864454181038501E-4 86-87 0.0 0.0 0.0 2.8596532115223265 1.864454181038501E-4 88-89 0.0 0.0 0.0 3.1286473384916564 1.864454181038501E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGG 315 0.0 28.750185 1 GGTATCA 1615 0.0 28.333246 1 GTACATG 2715 0.0 26.334135 1 TACATGG 2785 0.0 25.971928 2 ACCGTGC 225 1.0186341E-10 25.344011 8 GTATCAA 2155 0.0 24.772419 1 ACATGGG 2815 0.0 23.81 3 TCAACGC 2375 0.0 22.017504 4 GTGTAGC 805 0.0 21.90804 1 ATCAACG 2475 0.0 21.12692 3 CAACGCA 2490 0.0 21.000631 5 TATCAAC 2540 0.0 20.983122 2 TACCTGG 845 0.0 20.83679 2 CATGGGG 1720 0.0 20.452305 4 TAGGACA 635 0.0 20.21292 4 AACGCAG 2615 0.0 20.171963 6 GTACCTG 955 0.0 19.964306 1 GTATAGC 530 0.0 19.785381 1 GCACCGT 280 3.4689947E-8 18.66945 6 ACGCAGA 2810 0.0 18.602144 7 >>END_MODULE