FastQCFastQC Report
Fri 10 Feb 2017
SRR2031642_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031642_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072700
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11720.10925701500885615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20750.027.9390771
GGTATCA14550.027.7604331
CGCAACG350.00839750227.13998216-17
TCAACGC22600.025.0107444
GTACATG26050.024.8085561
CAACGCA22900.024.6807925
ATCAACG23250.024.3115183
AACGCAG23700.024.2484876
TACATGG25100.023.8443162
GCACCGT2052.6719135E-823.1682766
TATCAAC25000.022.609712
GTAGCAC3200.022.265343
ACATGGG24600.022.2050023
ACGCAGA26450.021.5478127
GTAGGAC2901.05501385E-1021.2928333
GTATAGG3401.8189894E-1220.9644091
ACCGTGC1856.5574422E-620.5383638
CGCAGAG27850.020.2940798
CATGGGG16200.020.2312224
GTGTACG1656.302455E-520.1597161
Produced by FastQC (version 0.11.5)