##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031642_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1072700 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.442398620303905 34.0 31.0 34.0 31.0 34.0 2 32.668546657965884 34.0 31.0 34.0 31.0 34.0 3 32.7855849724993 34.0 31.0 34.0 31.0 34.0 4 36.2383574158665 37.0 37.0 37.0 35.0 37.0 5 35.88913489326 37.0 35.0 37.0 35.0 37.0 6 35.94635592430316 37.0 36.0 37.0 35.0 37.0 7 35.92288990398061 37.0 36.0 37.0 35.0 37.0 8 36.04048569031416 37.0 36.0 37.0 35.0 37.0 9 37.95257760790528 39.0 39.0 39.0 35.0 39.0 10-11 37.92326372704391 39.0 38.0 39.0 35.0 39.0 12-13 37.93453621702247 39.0 38.0 39.0 35.0 39.0 14-15 39.40951384357229 41.0 39.0 41.0 36.0 41.0 16-17 39.37321898014356 41.0 39.0 41.0 36.0 41.0 18-19 39.35804838258599 41.0 39.0 41.0 36.0 41.0 20-21 39.37284841987508 41.0 39.0 41.0 36.0 41.0 22-23 39.31775053603057 41.0 39.0 41.0 36.0 41.0 24-25 39.250715018178425 41.0 39.0 41.0 36.0 41.0 26-27 39.13752680152885 41.0 39.0 41.0 36.0 41.0 28-29 39.05114104595879 40.0 39.0 41.0 35.5 41.0 30-31 38.956301389018364 40.0 38.5 41.0 35.0 41.0 32-33 38.82415027500699 40.0 38.0 41.0 35.0 41.0 34-35 38.71807774773934 40.0 38.0 41.0 35.0 41.0 36-37 38.58699916099562 40.0 38.0 41.0 34.5 41.0 38-39 38.47632655914981 40.0 38.0 41.0 34.0 41.0 40-41 38.364099002517015 40.0 38.0 41.0 34.0 41.0 42-43 38.215140300177126 40.0 38.0 41.0 33.5 41.0 44-45 38.16326372704391 40.0 38.0 41.0 33.0 41.0 46-47 38.19991283676704 40.0 38.0 41.0 33.5 41.0 48-49 38.335516919921695 40.0 38.0 41.0 34.0 41.0 50-51 38.26277384170784 40.0 38.0 41.0 34.0 41.0 52-53 38.160964855038685 40.0 38.0 41.0 33.5 41.0 54-55 38.04347534259345 40.0 37.5 41.0 33.0 41.0 56-57 37.92236925515056 40.0 37.0 41.0 33.0 41.0 58-59 37.716596438892516 40.0 37.0 41.0 33.0 41.0 60-61 37.425702433112704 39.5 36.0 41.0 32.0 41.0 62-63 37.21722429383797 39.0 36.0 41.0 32.0 41.0 64-65 36.9063666449147 39.0 35.0 41.0 32.0 41.0 66-67 36.60051458935396 38.5 35.0 40.5 31.0 41.0 68-69 36.29002610235854 37.5 35.0 40.0 31.0 41.0 70-71 35.9365829215997 37.0 35.0 39.5 31.0 41.0 72-73 35.54234222056493 36.5 35.0 39.0 31.0 41.0 74-75 35.16624918430129 36.0 34.5 39.0 30.0 40.5 76-77 34.54391908268855 35.0 34.0 37.0 29.5 39.0 78-79 34.492373450172465 35.0 34.0 37.0 30.0 39.0 80-81 34.22291274354433 35.0 34.0 37.0 30.0 39.0 82-83 33.953342966346604 35.0 34.0 36.0 30.0 37.5 84-85 33.69879323203132 35.0 34.0 36.0 29.5 37.0 86-87 33.47033187284423 35.0 34.0 35.5 29.0 37.0 88-89 33.25089540412044 35.0 34.0 35.0 29.0 36.0 90-91 33.11321851403002 35.0 34.0 35.0 29.0 36.0 92-93 32.99617180945278 35.0 34.0 35.0 29.0 36.0 94-95 32.87867670364501 35.0 34.0 35.0 29.0 36.0 96-97 32.79529598210124 35.0 33.5 35.0 29.0 35.0 98-99 32.705416705509464 35.0 33.0 35.0 29.0 35.0 100-101 32.266060874429016 34.5 32.5 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3162978419245306 1101 2 0.25216327570646513 1101 3 0.215706607001529 1101 4 0.1279702334524302 1101 5 0.26939326580449574 1101 6 0.0827476860369103 1101 7 0.2696246577298069 1101 8 0.18626476392689284 1101 9 0.27322578606528225 1101 10-11 0.13011717933650147 1101 12-13 0.12569962623903308 1101 14-15 0.18213825232194125 1101 16-17 0.19865700166146638 1101 18-19 0.08784740813122482 1101 20-21 0.10261955579574789 1101 22-23 0.1313754855555942 1101 24-25 0.11697231657917939 1101 26-27 0.2522223170952529 1101 28-29 0.166603192262059 1101 30-31 0.13262234089195601 1101 32-33 0.11251729465416105 1101 34-35 0.07516403733472288 1101 36-37 0.1813534076189498 1101 38-39 0.13438843735450945 1101 40-41 -0.07813636033728244 1101 42-43 -0.059365133457667696 1101 44-45 -0.03913966210056685 1101 46-47 0.14262135076991456 1101 48-49 0.14009598686829605 1101 50-51 0.273265487377067 1101 52-53 0.08483485811245828 1101 54-55 0.2528192182912363 1101 56-57 0.2855903616324724 1101 58-59 0.2678097746150385 1101 60-61 0.08230762631038147 1101 62-63 0.0849894938142981 1101 64-65 0.09960622619292536 1101 66-67 0.06155500640951317 1101 68-69 0.12339402586716375 1101 70-71 0.05137938548087817 1101 72-73 -0.031221020669164545 1101 74-75 0.030164494743196713 1101 76-77 0.0025571895942277934 1101 78-79 0.31137283796534376 1101 80-81 0.2906784380558918 1101 82-83 0.24101080376149042 1101 84-85 0.11531924626006429 1101 86-87 0.025463818400830007 1101 88-89 -0.011540712738884906 1101 90-91 0.0812594816445511 1101 92-93 -0.18522397826905035 1101 94-95 0.16218302524162453 1101 96-97 0.20811792943693064 1101 98-99 0.09723253909817942 1101 100-101 0.12451570728406125 1102 1 0.18335791320599526 1102 2 0.21658050658194128 1102 3 0.16692443476677 1102 4 0.10455340954724335 1102 5 0.01570278520958368 1102 6 -0.04899229694432705 1102 7 0.15690251977920866 1102 8 0.03430082396118195 1102 9 0.0217477439623579 1102 10-11 0.15345014269684754 1102 12-13 0.10695432797913895 1102 14-15 0.11986501493494472 1102 16-17 0.20614402177566404 1102 18-19 0.19888308586008208 1102 20-21 0.13165195309741762 1102 22-23 0.06859045219636073 1102 24-25 0.2228869514775056 1102 26-27 0.07661895280750741 1102 28-29 -0.378391689778212 1102 30-31 -0.31976645139287996 1102 32-33 -0.06822006151883642 1102 34-35 -0.08166689734037647 1102 36-37 0.03140952018939913 1102 38-39 0.04314631469612351 1102 40-41 0.15084855709708478 1102 42-43 0.06523992037340065 1102 44-45 0.12374834707476623 1102 46-47 0.06725353595373207 1102 48-49 0.02835921031964972 1102 50-51 0.07084031441468852 1102 52-53 -0.010923401534583377 1102 54-55 -0.1628824354880365 1102 56-57 -0.1715544334641521 1102 58-59 -0.04978367439651521 1102 60-61 0.17314339529924183 1102 62-63 0.04204857850111665 1102 64-65 -0.01960338817188756 1102 66-67 -0.10080887436051711 1102 68-69 -0.22797055785155607 1102 70-71 -0.1275777839790777 1102 72-73 -0.19546744734667953 1102 74-75 -0.10201575609849556 1102 76-77 -0.10370583653819665 1102 78-79 -0.32883397693716887 1102 80-81 -0.28639865198319825 1102 82-83 -0.6405633079950164 1102 84-85 -0.30664975000320993 1102 86-87 -0.2960198966213099 1102 88-89 -0.18138853051485881 1102 90-91 -0.07041957911734187 1102 92-93 -0.14022416325553877 1102 94-95 -0.08636236427827271 1102 96-97 0.1466968631747676 1102 98-99 -0.13392535304235764 1102 100-101 -0.24229709871443816 1103 1 0.14650227436359842 1103 2 0.1511073483935519 1103 3 0.10991742411737704 1103 4 0.10187286713159693 1103 5 0.02047681912268473 1103 6 0.06463116568575344 1103 7 0.08951538153114313 1103 8 0.038293740536879284 1103 9 0.0755647878456287 1103 10-11 0.11783895476777673 1103 12-13 0.03521525027979777 1103 14-15 -0.09691202700766866 1103 16-17 -0.06305642457710547 1103 18-19 0.07920353803483948 1103 20-21 -0.02404874796752665 1103 22-23 -0.1805374618322091 1103 24-25 0.03159568204053187 1103 26-27 0.0013781891561492898 1103 28-29 0.021250500261324134 1103 30-31 0.0778307637540081 1103 32-33 0.053004740169541265 1103 34-35 -0.03900503381758824 1103 36-37 -0.08245652546472115 1103 38-39 -0.10217304585501807 1103 40-41 -0.10322077313118427 1103 42-43 -0.029007223211486632 1103 44-45 -0.009604392900499192 1103 46-47 0.1021369748106764 1103 48-49 -0.028935261213234753 1103 50-51 0.10433860420437213 1103 52-53 0.20028393417605628 1103 54-55 0.21881467534814192 1103 56-57 0.03378239306472608 1103 58-59 0.21030777940354284 1103 60-61 0.09903670728527203 1103 62-63 0.012305473923575505 1103 64-65 0.06863133516216635 1103 66-67 0.11994410334609995 1103 68-69 0.006806144474815312 1103 70-71 0.30722075081945377 1103 72-73 0.17930755689852163 1103 74-75 0.03667502332076111 1103 76-77 -0.08321848724290248 1103 78-79 0.21872442492586686 1103 80-81 0.2874339172136047 1103 82-83 0.19210049619196212 1103 84-85 -0.06620509532015006 1103 86-87 -0.06119589060524788 1103 88-89 0.1731514107800507 1103 90-91 0.1254334640866901 1103 92-93 -0.09081364000395808 1103 94-95 0.09138064326605644 1103 96-97 -0.06792725442948466 1103 98-99 -0.020543260521186824 1103 100-101 0.22132029934005004 1104 1 -0.0013515230258036581 1104 2 0.11194269467781481 1104 3 0.1390059826176966 1104 4 0.08425529378646957 1104 5 0.13688321973961592 1104 6 -0.002286127547328931 1104 7 0.02060101416476101 1104 8 0.05827242026787616 1104 9 0.1596040715753162 1104 10-11 0.09318455859635577 1104 12-13 7.104362084149329E-4 1104 14-15 0.03244813811101466 1104 16-17 0.21549345691395416 1104 18-19 0.22991289060177422 1104 20-21 0.03933482408478284 1104 22-23 0.17501374650537826 1104 24-25 0.03590850028524528 1104 26-27 0.1403307742387696 1104 28-29 0.1463726948881643 1104 30-31 0.07825943724769502 1104 32-33 0.10188259090497809 1104 34-35 0.16995968567344732 1104 36-37 0.1379780451246404 1104 38-39 0.029947413664821454 1104 40-41 0.0391884172656205 1104 42-43 0.028684517452390423 1104 44-45 0.14306914615075073 1104 46-47 0.32495930212216706 1104 48-49 0.3577730253402578 1104 50-51 0.17828534889414982 1104 52-53 0.23559789781819518 1104 54-55 0.12032070286036145 1104 56-57 0.06992138307368378 1104 58-59 0.08761133240369645 1104 60-61 0.25324235715655874 1104 62-63 0.21408877833845708 1104 64-65 0.07030803823206355 1104 66-67 0.023744327889353656 1104 68-69 0.1450295309954157 1104 70-71 0.19749961540172478 1104 72-73 0.25302352101696357 1104 74-75 -0.17073547124143573 1104 76-77 -0.08279775214724339 1104 78-79 0.11196147683164526 1104 80-81 0.08920021077556584 1104 82-83 0.08379579779438728 1104 84-85 0.16785800360363368 1104 86-87 0.1871999859639999 1104 88-89 0.17020456402514839 1104 90-91 4.666352498929882E-4 1104 92-93 -0.06922176438731498 1104 94-95 -4.4734298916893067E-4 1104 96-97 0.022367486948844828 1104 98-99 0.1817855252441234 1104 100-101 0.010330721322610259 1105 1 0.17163396085892657 1105 2 0.10656098566537509 1105 3 0.09039149550054049 1105 4 0.08563910089783633 1105 5 0.0955971533339337 1105 6 0.08443636049094749 1105 7 0.172402948509621 1105 8 0.16582984080024943 1105 9 0.11119960757135772 1105 10-11 0.11182224420561226 1105 12-13 0.1607976224530745 1105 14-15 0.07684830357084849 1105 16-17 0.08494330296807817 1105 18-19 0.05752975727015297 1105 20-21 0.14569287404682285 1105 22-23 0.04901480408368997 1105 24-25 0.18914381572503203 1105 26-27 0.06161794939591658 1105 28-29 -0.052768662394200305 1105 30-31 0.04372396146538904 1105 32-33 0.21393471928133323 1105 34-35 0.11460953343334523 1105 36-37 -0.019810081000102286 1105 38-39 0.09094633476436087 1105 40-41 0.2607164260332837 1105 42-43 0.10077095315759266 1105 44-45 -0.041479336139076395 1105 46-47 0.1282358759095814 1105 48-49 0.18549916799392463 1105 50-51 0.2459367787570912 1105 52-53 0.26601819991032727 1105 54-55 0.2951111061900278 1105 56-57 0.12005294069891193 1105 58-59 0.08848074280378881 1105 60-61 0.12017352501299428 1105 62-63 0.2522484854574927 1105 64-65 0.14212261660799186 1105 66-67 0.1416712398459623 1105 68-69 0.24674575032350532 1105 70-71 0.3092335107867612 1105 72-73 0.39384188620557836 1105 74-75 0.2724506113690808 1105 76-77 0.11036860748055943 1105 78-79 0.22882228161463303 1105 80-81 0.16133728657151636 1105 82-83 0.2264025577667539 1105 84-85 0.07942654576604014 1105 86-87 0.27830097616434557 1105 88-89 0.36229522151477056 1105 90-91 0.2661064274869318 1105 92-93 0.17350045499195232 1105 94-95 0.0862255710655333 1105 96-97 0.053181182588041054 1105 98-99 0.023620530233507964 1105 100-101 0.11134277686252858 1106 1 0.15185901042122651 1106 2 0.13584965445866004 1106 3 0.12774066701465614 1106 4 0.08727690041322234 1106 5 0.10376983410375828 1106 6 0.05231978506242996 1106 7 0.17109658413900064 1106 8 0.21317305667976427 1106 9 0.10890607636954286 1106 10-11 0.22219116721475984 1106 12-13 0.07678799145767101 1106 14-15 0.0906003125777275 1106 16-17 0.05447880426118701 1106 18-19 0.1357790454494392 1106 20-21 0.1740810590467845 1106 22-23 0.14890017851362103 1106 24-25 0.06690696503197557 1106 26-27 0.15418727543018917 1106 28-29 0.2444266824207233 1106 30-31 0.22478953055482975 1106 32-33 0.07520514588426153 1106 34-35 -0.08969116900520646 1106 36-37 -0.03772593522574397 1106 38-39 0.05308063432462262 1106 40-41 -0.025294257396183184 1106 42-43 0.0728054754422729 1106 44-45 0.19271273715602177 1106 46-47 -0.021690086578075807 1106 48-49 0.18648786452578747 1106 50-51 0.24994408460748474 1106 52-53 0.1169784440097601 1106 54-55 0.19912340906260084 1106 56-57 0.20002671143145534 1106 58-59 0.19222147281773516 1106 60-61 0.2539014871253755 1106 62-63 0.15851301761384917 1106 64-65 0.3751189738808023 1106 66-67 0.3290325469853812 1106 68-69 0.22173514439887754 1106 70-71 0.12497239700150686 1106 72-73 0.19059922672919072 1106 74-75 0.31427772663916187 1106 76-77 0.2184445091881173 1106 78-79 0.2695770767163239 1106 80-81 0.31579218283741994 1106 82-83 0.40718462233235897 1106 84-85 0.32972636709700254 1106 86-87 0.19936842168660007 1106 88-89 0.3365172157549381 1106 90-91 0.28661665404579395 1106 92-93 0.170141742224871 1106 94-95 0.2773682982566754 1106 96-97 0.05305166793147009 1106 98-99 0.26427660865208225 1106 100-101 0.30963430517626733 1107 1 0.0027230650671512535 1107 2 -0.01955914985915541 1107 3 0.028289282144406513 1107 4 0.024423496578783954 1107 5 0.061565615383898376 1107 6 0.1145216966107725 1107 7 0.0571714802217258 1107 8 -0.05347923744299976 1107 9 -0.10269086454795939 1107 10-11 -0.0491487510130284 1107 12-13 -0.017325695364597493 1107 14-15 -0.05610742930651469 1107 16-17 0.08229535008501188 1107 18-19 -0.12208377618350852 1107 20-21 -0.08671347787363715 1107 22-23 -0.01030883987944975 1107 24-25 -0.06694964236685053 1107 26-27 -0.010961291286783137 1107 28-29 -0.03642180827305452 1107 30-31 -0.06994406677944198 1107 32-33 0.11387059844919634 1107 34-35 -0.008276853951471708 1107 36-37 0.10341670092574162 1107 38-39 0.009811712681461415 1107 40-41 -0.11017311198007462 1107 42-43 -0.08671321453817171 1107 44-45 -0.13689271760261335 1107 46-47 -5.955417149436926E-4 1107 48-49 0.06091674501808342 1107 50-51 0.008751049996128302 1107 52-53 -0.10445376107887228 1107 54-55 0.029053191621144947 1107 56-57 -0.19976356289502206 1107 58-59 -0.12802008047603408 1107 60-61 0.22766497556871457 1107 62-63 0.01304017983068917 1107 64-65 -0.20051417450058295 1107 66-67 0.002677461941523518 1107 68-69 0.006047229060719417 1107 70-71 -0.11081533187179815 1107 72-73 -0.07447965206110041 1107 74-75 0.015749588602226083 1107 76-77 -0.10231663047896689 1107 78-79 -0.007860771486910778 1107 80-81 0.11731160657405582 1107 82-83 0.042367853355877116 1107 84-85 0.03435593368916301 1107 86-87 -0.2313171486575385 1107 88-89 -0.29079326523359583 1107 90-91 -0.12831585173158544 1107 92-93 -0.004215180991707257 1107 94-95 -0.08900439315346631 1107 96-97 -0.061994872635033005 1107 98-99 0.0067650633388538495 1107 100-101 0.08794065216729763 1108 1 0.08278037534758198 1108 2 -0.0016255396334514671 1108 3 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21 2801.0 22 3563.0 23 3967.0 24 4084.0 25 4680.0 26 5585.0 27 7119.0 28 8867.0 29 11497.0 30 14468.0 31 18246.0 32 23220.0 33 31067.0 34 43703.0 35 66814.0 36 120225.0 37 248437.0 38 349310.0 39 99405.0 40 140.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.60022608650234 18.35171374314932 12.724361007166868 24.323699163181477 2 15.870691198186238 19.121776322658594 40.528373581134495 24.47915889802067 3 19.49635170547398 23.428697679578406 29.596352358037205 27.478598256910413 4 12.53947166011719 15.687601680022004 37.6204660615977 34.152460598263104 5 13.187470867903423 37.602778036729745 35.7213573226438 13.488393772723034 6 33.31844877412138 36.55001398340636 16.978465554209006 13.15307168826326 7 28.917777570616206 31.682297007551043 21.02563624498928 18.37428917684348 8 25.97147385103011 36.93334576302787 19.16519064044001 17.929989745502002 9 25.73310338398434 15.68089866691526 19.638389111587582 38.94760883751282 10-11 25.057518411485034 26.027174419688638 28.55556073459495 20.35974643423138 12-13 25.400298312668966 23.10040085764892 28.604549268201733 22.894751561480376 14-15 22.369814486808988 25.036170411112145 25.74797240607812 26.846042696000744 16-17 22.602824648084272 28.322270905192504 26.410459587955625 22.664444858767595 18-19 22.47161368509369 27.66048289363289 27.784562319381003 22.08334110189242 20-21 23.0811503682297 26.803206861191388 27.922392094714272 22.19325067586464 22-23 22.70916798929776 26.77117200263827 27.602833110758407 22.91682689730556 24-25 22.587489512445234 26.769926354059848 27.84622914141885 22.796354992076072 26-27 22.676703645007922 26.940943413815603 27.62874056120071 22.753612379975763 28-29 22.57192982619727 27.01185088556452 27.6610177709668 22.755201517271413 30-31 22.808487308338357 26.899413563972995 27.672796762317418 22.61930236537123 32-33 22.51305117926727 26.991190453994594 27.746387620024237 22.749370746713897 34-35 22.78768528013424 27.005779807961222 27.74820546285075 22.45832944905379 36-37 22.80595693110842 26.989512445231657 27.56353127621889 22.64099934744104 38-39 22.74126037102638 27.10249836860259 27.591684534352567 22.56455672601846 40-41 22.95073179826606 26.845716416519068 27.65181318169106 22.55173860352382 42-43 22.70145427426121 26.857136198377923 27.71986575929897 22.7215437680619 44-45 22.798499114384263 27.17950032627948 27.512864733849167 22.50913582548709 46-47 22.7651719958982 26.921832758459963 27.350843665516923 22.962151580124917 48-49 22.704099474335507 27.021411712703884 27.45186537692205 22.82262343603856 50-51 22.7647631660772 27.15265531176564 27.339728215190245 22.742853306966914 52-53 22.77132469469563 27.177169758553184 27.300550013983404 22.750955532767783 54-55 22.70244772373765 27.101526488768197 27.42291462448861 22.773111163005545 56-57 22.715484291973524 27.12846089307355 27.364081290202293 22.79197352475063 58-59 22.750349585158943 27.102591591311644 27.390882819054724 22.75617600447469 60-61 22.844457909946865 27.183182623287035 27.309639228115966 22.662720238650135 62-63 22.692971007737484 27.244103663652464 27.32632609303626 22.736599235573784 64-65 22.6125664211802 27.059662533793233 27.393865945744384 22.933905099282185 66-67 22.609303626363385 27.039433205928965 27.323296354992078 23.02796681271558 68-69 22.724433672042508 27.037242472266243 27.31840216276685 22.919921692924397 70-71 22.715670737391626 26.851263167707657 27.379556259904913 23.053509834995804 72-73 22.619884403840775 27.067260184580967 27.288244616388553 23.02461079518971 74-75 22.52181411391815 27.10543488393773 27.27729094807495 23.09546005406917 76-77 22.58231565209285 27.018737764519436 27.20112799477953 23.197818588608186 78-79 22.611080943136347 26.821074906951864 27.227293808366294 23.340550341545494 80-81 22.60203184968433 27.020067231074535 27.13082928425617 23.24707163498496 82-83 22.607093346410114 26.99030149006329 27.23446564967338 23.168139513853216 84-85 22.580218141139184 27.0326745595227 27.159131164351635 23.227976134986484 86-87 22.498088934464437 27.050293651533515 27.2818588608185 23.169758553183556 88-89 22.647105434883937 26.891628600727135 27.17395357509089 23.287312389298034 90-91 22.64244429943134 26.882492775240046 27.105761163419405 23.3693017619092 92-93 22.597184674186632 26.81667754264939 27.195254964109257 23.390882819054724 94-95 22.572107765451662 26.91423510767223 27.146779155402257 23.36687797147385 96-97 22.51855131910133 26.821571734874617 27.253658991330287 23.406217954693766 98-99 22.451990304838258 26.813927472732356 27.165423697212642 23.568658525216744 100-101 22.41535378018085 26.783723314999534 27.129439731518595 23.671483173301016 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 18.0 1 13.5 2 21.0 3 39.0 4 33.5 5 30.5 6 54.0 7 107.5 8 186.5 9 245.5 10 240.5 11 205.5 12 216.0 13 238.5 14 257.5 15 293.5 16 337.0 17 375.5 18 445.5 19 522.0 20 598.5 21 738.0 22 889.0 23 1142.0 24 1506.0 25 1931.0 26 2658.0 27 3686.0 28 4931.5 29 6687.0 30 8767.5 31 11104.0 32 13867.5 33 17096.0 34 20184.0 35 22575.0 36 25354.5 37 28091.0 38 30397.5 39 32852.5 40 34868.0 41 36562.0 42 38037.0 43 39711.0 44 41996.5 45 44081.5 46 45276.5 47 45894.0 48 46152.0 49 46203.0 50 45821.5 51 44676.0 52 43078.0 53 41195.0 54 38778.0 55 35987.5 56 32553.0 57 28323.5 58 24443.0 59 20454.5 60 16220.5 61 12666.0 62 9538.0 63 6726.0 64 4636.0 65 3095.5 66 2028.0 67 1405.5 68 985.0 69 710.5 70 510.0 71 364.0 72 252.0 73 166.0 74 116.5 75 88.5 76 60.5 77 33.0 78 20.0 79 14.0 80 11.5 81 7.5 82 3.5 83 4.5 84 5.5 85 4.0 86 2.5 87 1.5 88 1.0 89 1.0 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05071315372424723 2 0.0011186725086231007 3 6.525589633634754E-4 4 0.00885615735993288 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.002563624498927939 24-25 0.0 26-27 0.0 28-29 0.023165843199403374 30-31 0.019064044001118673 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.031975389204810294 50-51 0.011513004567912743 52-53 0.0 54-55 8.856157359932879E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.3305677262981263E-4 80-81 0.012165563531276218 82-83 0.005686585252167428 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1072700.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.56172455817064 #Duplication Level Percentage of deduplicated Percentage of total 1 76.23905790593977 51.508422308155254 2 14.414827907938102 19.477812653390906 3 4.695645293619457 9.51737681851164 4 2.045370576832424 5.527550541253555 5 0.9795648706184089 3.30905459877905 6 0.5430838350145807 2.2015008283950053 7 0.32124195679033934 1.5192562420837663 8 0.19917882857575764 1.076549212324354 9 0.1327920869974902 0.8074496164706161 >10 0.4195231520116504 4.241298853409918 >50 0.00735775372335382 0.3200399422983285 >100 0.0019400983033942974 0.23153669224397935 >500 1.385778784395352E-4 0.05355384579108868 >1k 2.771557568790704E-4 0.2085978468925622 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1172 0.10925701500885615 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 9.322270905192505E-5 0.0 16-17 0.0 0.0 0.0 4.195021907336628E-4 0.0 18-19 0.0 0.0 0.0 6.059476088375129E-4 0.0 20-21 0.0 0.0 0.0 8.85615735993288E-4 0.0 22-23 0.0 0.0 0.0 0.001771231471986576 0.0 24-25 0.0 0.0 0.0 0.002889903980609677 0.0 26-27 0.0 0.0 0.0 0.00424163326186259 0.0 28-29 0.0 0.0 0.0 0.0064789782791087915 0.0 30-31 0.0 0.0 0.0 0.009834995804978094 0.0 32-33 0.0 0.0 0.0 0.01827165097417731 0.0 34-35 0.0 0.0 0.0 0.031788943786706436 0.0 36-37 0.0 0.0 0.0 0.05318355551412324 0.0 38-39 0.0 0.0 0.0 0.08418010627388832 0.0 40-41 0.0 0.0 0.0 0.12142257854013239 0.0 42-43 0.0 0.0 0.0 0.15600820359839657 0.0 44-45 0.0 0.0 0.0 0.1979118113172369 0.0 46-47 0.0 0.0 0.0 0.24853174233243217 0.0 48-49 0.0 0.0 0.0 0.30376619744569777 0.0 50-51 0.0 0.0 0.0 0.3628227836300923 0.0 52-53 0.0 0.0 0.0 0.4220192038780647 4.661135452596253E-5 54-55 0.0 0.0 0.0 0.4860165936422113 9.322270905192505E-5 56-57 0.0 0.0 0.0 0.5632049967372053 9.322270905192505E-5 58-59 0.0 0.0 0.0 0.6473851030110935 9.322270905192505E-5 60-61 0.0 0.0 0.0 0.7366924582828377 1.864454181038501E-4 62-63 0.0 0.0 0.0 0.8305211149436003 1.864454181038501E-4 64-65 0.0 0.0 0.0 0.9284049594481216 1.864454181038501E-4 66-67 0.0 0.0 0.0 1.043581616481775 1.864454181038501E-4 68-69 0.0 0.0 0.0 1.1735340729001584 1.864454181038501E-4 70-71 0.0 0.0 0.0 1.3180292719306423 1.864454181038501E-4 72-73 0.0 0.0 0.0 1.470261955812436 1.864454181038501E-4 74-75 0.0 0.0 0.0 1.6212827444765545 1.864454181038501E-4 76-77 0.0 0.0 0.0 1.7883378390976041 1.864454181038501E-4 78-79 0.0 0.0 0.0 1.9825207420527642 1.864454181038501E-4 80-81 0.0 0.0 0.0 2.1976321431900807 1.864454181038501E-4 82-83 0.0 0.0 0.0 2.4174512911345203 1.864454181038501E-4 84-85 0.0 0.0 0.0 2.6423044653677636 1.864454181038501E-4 86-87 0.0 0.0 0.0 2.8760604083154657 1.864454181038501E-4 88-89 0.0 0.0 0.0 3.1468723781113077 1.864454181038501E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2075 0.0 27.939077 1 GGTATCA 1455 0.0 27.760433 1 CGCAACG 35 0.008397502 27.139982 16-17 TCAACGC 2260 0.0 25.010744 4 GTACATG 2605 0.0 24.808556 1 CAACGCA 2290 0.0 24.680792 5 ATCAACG 2325 0.0 24.311518 3 AACGCAG 2370 0.0 24.248487 6 TACATGG 2510 0.0 23.844316 2 GCACCGT 205 2.6719135E-8 23.168276 6 TATCAAC 2500 0.0 22.60971 2 GTAGCAC 320 0.0 22.26534 3 ACATGGG 2460 0.0 22.205002 3 ACGCAGA 2645 0.0 21.547812 7 GTAGGAC 290 1.05501385E-10 21.292833 3 GTATAGG 340 1.8189894E-12 20.964409 1 ACCGTGC 185 6.5574422E-6 20.538363 8 CGCAGAG 2785 0.0 20.294079 8 CATGGGG 1620 0.0 20.231222 4 GTGTACG 165 6.302455E-5 20.159716 1 >>END_MODULE