FastQCFastQC Report
Fri 10 Feb 2017
SRR2031641_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031641_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962248
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57790.6005728253007541No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC56710.5893491075065888No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT54330.5646153590342614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49360.5129654725185191No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC37950.3943889724894206No Hit
TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC32870.34159592953168No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT31390.3262152792211571No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31090.323097579833889No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC25920.2693692270599679No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT25160.2614710552788886No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT23800.24733748472327302No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT22310.2318529110998412No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC21060.21886249698622393No Hit
CTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT21020.2184468037345882No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC18530.19256989882026257No Hit
ATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTT18290.19007573931044802No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA17640.18332072397136703No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG16730.17386370249665367No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA16460.17105777304811234No Hit
CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG15800.1641988343961224No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT15670.16284783132830621No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG14800.1538065031052286No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA14780.1535986564794107No Hit
ATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC14740.15318296322777497No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA14350.14912995402432638No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC14150.1470514877661476No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT14090.14642794788869398No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14010.14559656138542246No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT13950.14497302150796884No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC13790.14331024850142582No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC13240.1375944662914342No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13000.13510030678161972No Hit
AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA12870.13374930371380353No Hit
CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA12800.13302184052344093No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA12390.12876098469417446No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12340.12824136812962977No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG12280.12761782825217616No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG11940.12408443561327226No Hit
ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA11820.12283735585836499No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT11710.12169419941636668No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC11650.12107065953891305No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC11520.11971965647109685No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA11400.11847257671618958No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA11220.11660195708382869No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC11080.11514703070310357No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG11030.11462741413855887No Hit
TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC10850.112756794506198No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC10730.11150971475129073No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA10680.11099009818674604No Hit
CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT10640.1105744049351103No Hit
ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA10410.10818416873820469No Hit
CTACTATACAATCAAAGATATCCTAGGTATCCTAATCATATTCTTAATTC10330.10735278223493319No Hit
GGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCT10290.10693708898329744No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC10280.10683316567038852No Hit
GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA10260.10662531904457064No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA10190.10589785585420805No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT9960.10350761965730249No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC9960.10350761965730249No Hit
GATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGC9930.10319584971857566No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT9910.1029880030927578No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC9720.10101346014748797No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC9670.10049384358294328No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGC250.005010579757.033
GCGGCTC250.00501988257.003287
TACCTGG1500.044.389032
GTGTAGC1650.040.4084171
GGATGAC5000.036.1020778
GTCGGGA1451.6370905E-1136.0797542
GCCCTGG1850.035.9480136
TGGGTAT4000.035.627056
CATATAG2850.035.0184173
GGTCGGG1502.3646862E-1134.924421
GCTACAC1106.978189E-834.5636373
GTACATG52900.033.5800131
GACATAT2700.033.5133321
ACCTGGG2000.033.2674983
CATGGGG18150.031.9451774
ACATGGG53150.031.7429433
CATGGGT5400.031.6833344
TATACAG600.0039404731.6833295
CTGGCTG2250.031.6684889
ATGGGTA4250.031.3105875
Produced by FastQC (version 0.11.5)