FastQCFastQC Report
Fri 10 Feb 2017
SRR2031641_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031641_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962248
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT64930.674774070717736No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63100.6557561044554003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59970.6232281075149027No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC56760.5898687240711334No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC40690.42286396022646966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37330.38794572708906644No Hit
TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC33570.34887056143530565No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27680.287659730131941No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC26470.2750850092699595No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT26460.27498108595705056No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT22900.23798438656146859No Hit
CTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT20860.2167840307280452No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC20560.21366633134077703No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20530.21335456140205022No Hit
ATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTT19630.20400146324024573No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA18120.18830904299099607No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG17670.18363249391009387No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC17500.1818657975906419No Hit
CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG16590.17240877611592853No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA15710.16326352457994198No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15450.16056151844430958No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA14880.15463788960850008No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14860.1544300429826822No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT14770.15349473316650178No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG14670.15245550003741237No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA14660.15235157672450345No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC14580.15152019022123195No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT13990.1453887147596046No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC13880.14424555831760627No Hit
ATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC13590.14123178224324706No Hit
CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA13230.13749054297852528No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA12870.13374930371380353No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC12820.1332296871492588No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC12570.13063160432653537No Hit
ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA12410.12896883131999234No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT12180.12657859512308678No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA11960.12429228223909013No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA11950.12418835892618119No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA11890.12356481904872757No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG11700.12159027610345774No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC11320.11764119021291808No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11300.11743334358710021No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG11300.11743334358710021No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11130.11566664726764826No Hit
AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA11100.11535487732892144No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC11070.11504310739019463No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG11000.11431564419983205No Hit
TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC10850.112756794506198No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT10830.1125489478803801No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT10540.1095351718060209No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT10520.10932732518020302No Hit
CTACTATACAATCAAAGATATCCTAGGTATCCTAATCATATTCTTAATTC10490.10901555524147621No Hit
GATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGC10490.10901555524147621No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC10320.10724885892202426No Hit
GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA10190.10589785585420805No Hit
GGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCT10090.10485862272511869No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10090.10485862272511869No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG9990.1038193895960293No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA9970.10361154297021141No Hit
CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT9890.10278015646693991No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG9810.1019487699636684No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC9770.10153307671203265No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC9690.10070169020876116No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC9650.10028599695712541No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG1500.047.4952962
GACATAT3850.044.427351
ACATATA3800.043.7456672
GGCAACA459.6289837E-442.218048
GGATGAC5350.040.837088
ACAGTAC608.294584E-539.579428
GTGTAGC1003.0227966E-838.0100631
AGCGGGT250.001614810238.00611526-27
CATATAG4300.037.554423
GGGTATG3050.035.8161247
ACCTGGG2000.035.6214753
CTGGCTG1502.5465852E-1134.8298849
TGGGTAT3200.034.1372456
GGATAGC851.6330338E-533.5260938
GCCTATC851.6330338E-533.5260937
CATTCCG1850.033.3750729
GTATTAC1509.513315E-1031.6750531
CATCGCC300.00395151331.66682480-81
GACTGTG600.003952550231.6635347
GGGATGC1351.1979864E-831.6635327
Produced by FastQC (version 0.11.5)