##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031641_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 962248 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52948096540601 34.0 31.0 34.0 31.0 34.0 2 32.720803784471364 34.0 31.0 34.0 31.0 34.0 3 32.80248127301901 34.0 31.0 34.0 31.0 34.0 4 36.239091169843945 37.0 37.0 37.0 35.0 37.0 5 36.02162540218322 37.0 35.0 37.0 35.0 37.0 6 36.10951958330909 37.0 37.0 37.0 35.0 37.0 7 36.05117703544201 37.0 37.0 37.0 35.0 37.0 8 36.16019882608226 37.0 37.0 37.0 35.0 37.0 9 38.01630660702854 39.0 39.0 39.0 35.0 39.0 10-11 37.99341541889409 39.0 38.5 39.0 35.0 39.0 12-13 38.02509955853377 39.0 39.0 39.0 35.0 39.0 14-15 39.406004481173255 41.0 39.0 41.0 36.0 41.0 16-17 39.39834065646278 41.0 39.0 41.0 36.0 41.0 18-19 39.50059548058297 41.0 39.5 41.0 37.0 41.0 20-21 39.54167220924336 41.0 40.0 41.0 37.0 41.0 22-23 39.4539728843292 41.0 39.0 41.0 36.0 41.0 24-25 39.38008081076812 41.0 39.0 41.0 36.0 41.0 26-27 39.23857415136223 41.0 39.0 41.0 36.0 41.0 28-29 39.15166308477649 41.0 39.0 41.0 35.0 41.0 30-31 38.98601140246589 40.0 38.5 41.0 35.0 41.0 32-33 38.76598496437509 40.0 38.0 41.0 35.0 41.0 34-35 38.62017847789759 40.0 38.0 41.0 35.0 41.0 36-37 38.434252916088155 40.0 38.0 41.0 34.5 41.0 38-39 38.27824531721552 40.0 38.0 41.0 34.0 41.0 40-41 38.19145895860527 40.0 38.0 41.0 34.0 41.0 42-43 38.01932142233603 40.0 38.0 41.0 34.0 41.0 44-45 37.864827466515905 40.0 38.0 41.0 33.0 41.0 46-47 37.68024511352583 40.0 38.0 41.0 33.0 41.0 48-49 37.647009918440986 40.0 38.0 41.0 33.0 41.0 50-51 37.530654779225316 40.0 38.0 41.0 33.0 41.0 52-53 37.50334685029223 40.0 38.0 41.0 32.5 41.0 54-55 37.4272323766846 40.0 38.0 41.0 32.0 41.0 56-57 37.32816591980446 40.0 38.0 41.0 32.0 41.0 58-59 37.184863985168064 40.0 37.0 41.0 31.0 41.0 60-61 36.94402638405068 40.0 37.0 41.0 31.0 41.0 62-63 36.77588885609531 40.0 36.5 41.0 31.0 41.0 64-65 36.51635441175248 39.0 36.0 41.0 30.0 41.0 66-67 36.26519566681354 39.0 36.0 41.0 29.5 41.0 68-69 35.957098897581496 39.0 35.0 41.0 29.0 41.0 70-71 35.59539744431789 37.5 35.0 40.0 29.0 41.0 72-73 35.221614386312055 37.0 35.0 39.5 28.5 41.0 74-75 34.82280451609149 37.0 35.0 39.0 28.0 41.0 76-77 34.17014532636077 36.0 34.0 39.0 27.5 40.0 78-79 34.07595858863827 36.0 34.0 38.0 27.0 40.0 80-81 33.7433961930812 35.5 34.0 37.0 26.5 39.0 82-83 33.38729568676682 35.0 34.0 37.0 26.0 39.0 84-85 33.02574232422411 35.0 34.0 36.0 26.0 38.0 86-87 32.7777677895927 35.0 34.0 36.0 26.0 37.0 88-89 32.50092024093581 35.0 34.0 36.0 25.5 37.0 90-91 32.310094694922725 35.0 34.0 35.5 25.0 36.5 92-93 32.15393796609606 35.0 34.0 35.0 25.0 36.0 94-95 31.9885892202426 35.0 34.0 35.0 24.0 36.0 96-97 31.865595459798307 35.0 33.0 35.0 24.0 36.0 98-99 31.801634298018804 35.0 33.0 35.0 23.5 35.5 100-101 31.40553007124982 34.5 32.5 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20055811176764848 1101 2 0.2839981427123064 1101 3 0.18498712599999578 1101 4 0.12787318199806919 1101 5 0.2653367847761814 1101 6 0.14082574787727253 1101 7 0.2888765862479943 1101 8 0.1160063046757287 1101 9 0.21222764094763846 1101 10-11 0.21349099115254688 1101 12-13 0.1880544503484458 1101 14-15 0.3524563755124319 1101 16-17 0.2733116328456262 1101 18-19 0.22238945134903076 1101 20-21 0.11376909354036968 1101 22-23 0.12334368883991687 1101 24-25 0.19198213234520267 1101 26-27 0.26205341719571607 1101 28-29 0.24347363672363542 1101 30-31 0.1825403784495805 1101 32-33 0.06068828982557761 1101 34-35 0.14810931093239077 1101 36-37 0.28211097880190295 1101 38-39 0.11578075656115061 1101 40-41 0.20437629982708927 1101 42-43 0.13013573064887396 1101 44-45 0.2775049167770689 1101 46-47 0.5524206829050442 1101 48-49 0.4424643933297858 1101 50-51 0.5157129604403465 1101 52-53 0.4423125588984931 1101 54-55 0.5508209915026114 1101 56-57 0.4779358473849342 1101 58-59 0.39283603435728054 1101 60-61 0.48698208118673136 1101 62-63 0.21432770840494442 1101 64-65 0.31410715496411257 1101 66-67 0.33952025865416857 1101 68-69 0.2252564157396364 1101 70-71 -0.04682158477203302 1101 72-73 0.12843844198884113 1101 74-75 0.2399705935330232 1101 76-77 0.263213676247787 1101 78-79 0.2550388446133738 1101 80-81 0.31098252260619574 1101 82-83 0.32555579049495265 1101 84-85 0.2577331894546191 1101 86-87 0.34091225288284477 1101 88-89 0.45757885666579057 1101 90-91 0.38580760941341197 1101 92-93 0.13010899385071184 1101 94-95 0.0838822089584923 1101 96-97 0.2835207226128489 1101 98-99 0.22761676219024451 1101 100-101 0.31109873497772256 1102 1 0.22980675772533488 1102 2 0.17818887245494608 1102 3 0.14869729351330108 1102 4 0.23682925824734014 1102 5 0.10167789854570941 1102 6 0.12896278878075407 1102 7 0.18635564500335988 1102 8 0.19760015358574634 1102 9 0.37386358046902046 1102 10-11 0.2686733144277582 1102 12-13 0.1449673504215383 1102 14-15 0.2784964448420624 1102 16-17 0.24772094073146178 1102 18-19 0.3473068268795316 1102 20-21 0.301009877478009 1102 22-23 0.3093848570541837 1102 24-25 0.34094260413301214 1102 26-27 0.26363264761018 1102 28-29 -0.10052636993627573 1102 30-31 0.1797407201027994 1102 32-33 0.46320889807486765 1102 34-35 0.424645293576674 1102 36-37 0.4616653977333556 1102 38-39 0.396694795478318 1102 40-41 0.46224373105106764 1102 42-43 0.5342946759860254 1102 44-45 0.6624350460292092 1102 46-47 0.8243746496288153 1102 48-49 0.7415574805129665 1102 50-51 0.6963704264456823 1102 52-53 0.6803584423268134 1102 54-55 0.5780941425875881 1102 56-57 0.44603156356634344 1102 58-59 0.5987079077391328 1102 60-61 0.9379726590835062 1102 62-63 0.7003826959259385 1102 64-65 0.7996618169821659 1102 66-67 0.8242099652686221 1102 68-69 0.6297242142574717 1102 70-71 0.7628474345067318 1102 72-73 0.862441761952411 1102 74-75 0.8144100374973959 1102 76-77 0.663138585797924 1102 78-79 0.548873935329631 1102 80-81 0.5788048496109539 1102 82-83 0.34853246916610203 1102 84-85 0.3877678709195962 1102 86-87 0.21063781477086252 1102 88-89 0.3348326107720325 1102 90-91 0.6425436889593428 1102 92-93 0.6876281119458483 1102 94-95 0.6075157209438231 1102 96-97 0.5926794265283704 1102 98-99 0.491854394326964 1102 100-101 0.38176534170522913 1103 1 0.22464003677296773 1103 2 0.19226323747196972 1103 3 0.20972239905108125 1103 4 0.12304324607457318 1103 5 0.029923384849745105 1103 6 -0.03112093892219292 1103 7 0.123686002203236 1103 8 0.21419241691607738 1103 9 0.19903968965352448 1103 10-11 0.2230637499267658 1103 12-13 0.11651525914493277 1103 14-15 0.21652852449514626 1103 16-17 0.13962694345212867 1103 18-19 0.2826741175123715 1103 20-21 0.18185037971649365 1103 22-23 0.06683876211169348 1103 24-25 0.07449563487148936 1103 26-27 0.1350473967144623 1103 28-29 0.2909381623445597 1103 30-31 0.1913291195688842 1103 32-33 0.24587132407069134 1103 34-35 0.16846231246171328 1103 36-37 0.17915784563505355 1103 38-39 0.11872808218981845 1103 40-41 -0.06606865439014342 1103 42-43 0.09425482389858075 1103 44-45 0.004790183052691077 1103 46-47 -0.08111155861111286 1103 48-49 -0.31021191385026725 1103 50-51 -0.21708220768788067 1103 52-53 -0.19979790047778323 1103 54-55 -0.10035890667189307 1103 56-57 -0.11070896357970383 1103 58-59 -0.2049798473238056 1103 60-61 -0.14383029661298252 1103 62-63 -0.18741116531982271 1103 64-65 -0.29773151162512335 1103 66-67 -0.15349617667889248 1103 68-69 -0.21742802199387512 1103 70-71 -0.1515073001639422 1103 72-73 -0.17535146063810458 1103 74-75 -0.34544377431644335 1103 76-77 -0.466050992625739 1103 78-79 -0.15577914713992413 1103 80-81 -0.09644773853943889 1103 82-83 -0.1970403726581509 1103 84-85 -0.3438212949327877 1103 86-87 -0.37130245180384236 1103 88-89 -0.31417763325813297 1103 90-91 -0.10643206768745728 1103 92-93 -0.3874370308894015 1103 94-95 -0.2780508301262401 1103 96-97 -0.19517287167253983 1103 98-99 -0.239694057253395 1103 100-101 -0.1448051115484006 1104 1 0.1597224937375401 1104 2 0.15091730370255618 1104 3 0.18637364644607146 1104 4 0.1950982254151583 1104 5 0.029113270771226496 1104 6 -0.012211250078273395 1104 7 -0.012199341056252422 1104 8 0.11750274180495524 1104 9 0.12828269978446372 1104 10-11 0.1464944174125833 1104 12-13 0.19508126687865968 1104 14-15 0.23081393762321056 1104 16-17 0.2929191988819966 1104 18-19 0.22959056433395375 1104 20-21 0.14552323818444535 1104 22-23 0.21428404176668892 1104 24-25 0.2881097675976818 1104 26-27 0.2010912693609228 1104 28-29 0.08258073639922259 1104 30-31 0.13707255734369284 1104 32-33 0.25477053262838467 1104 34-35 0.21381514876446062 1104 36-37 0.2529053309686802 1104 38-39 0.23113517505478853 1104 40-41 0.13609998184755057 1104 42-43 0.26159608370126364 1104 44-45 0.18335107110636528 1104 46-47 0.22340550498133638 1104 48-49 0.33057627595133 1104 50-51 0.47542378534934926 1104 52-53 0.441189053372824 1104 54-55 0.5081481946254982 1104 56-57 0.53287848657871 1104 58-59 0.3689377920820647 1104 60-61 0.4478917830612872 1104 62-63 0.33902897056250936 1104 64-65 0.3735940638871469 1104 66-67 0.31190683248834006 1104 68-69 0.4084317830968942 1104 70-71 0.3759643006696578 1104 72-73 0.2621206822792672 1104 74-75 0.4022523696610065 1104 76-77 0.26658576306311943 1104 78-79 0.23284823652287656 1104 80-81 0.3800423895127949 1104 82-83 0.5344595129019396 1104 84-85 0.52942446175593 1104 86-87 0.5858262509294647 1104 88-89 0.47292950658743393 1104 90-91 0.4950475198470272 1104 92-93 0.5277946195749834 1104 94-95 0.4077438646805973 1104 96-97 0.29689915319103477 1104 98-99 0.6028833101468933 1104 100-101 0.5684702188215418 1105 1 0.11521127695758793 1105 2 0.10069996947460425 1105 3 0.01993938640347892 1105 4 0.08807351640964356 1105 5 0.07212502724020453 1105 6 0.05344119019127902 1105 7 0.1092131855635472 1105 8 0.06662902903619994 1105 9 0.018997131360649178 1105 10-11 0.10524321405192438 1105 12-13 0.20585170942365494 1105 14-15 0.18524668070206474 1105 16-17 0.050092687023429505 1105 18-19 0.09570684543901109 1105 20-21 0.08655127284122699 1105 22-23 0.04271690153505858 1105 24-25 0.029839397983636218 1105 26-27 0.043993629406422485 1105 28-29 0.07523804845617121 1105 30-31 -0.0540823771643133 1105 32-33 0.017126494004159554 1105 34-35 0.1703789341724331 1105 36-37 0.0794755343001654 1105 38-39 0.08981541660209302 1105 40-41 0.15412705381581304 1105 42-43 0.057552791889946775 1105 44-45 0.06293769848756625 1105 46-47 0.28634783118688034 1105 48-49 0.16906339634574863 1105 50-51 0.11129997777037204 1105 52-53 0.1339280480926206 1105 54-55 0.0419356446677952 1105 56-57 -0.09259921113709169 1105 58-59 -0.056324030024761385 1105 60-61 0.008514905089349156 1105 62-63 -0.02142932752086324 1105 64-65 -0.1653991972931621 1105 66-67 -0.10689184892009962 1105 68-69 -0.10204110117627607 1105 70-71 -0.04340324214080482 1105 72-73 0.05351774753788874 1105 74-75 0.11110181100993088 1105 76-77 0.10514873164446925 1105 78-79 0.16180911636184447 1105 80-81 0.049669566501620466 1105 82-83 -0.0437166299260312 1105 84-85 -0.01688669084479244 1105 86-87 -0.03612742333174879 1105 88-89 0.06796436758104818 1105 90-91 -0.1512636327536896 1105 92-93 -0.07694880934651138 1105 94-95 -0.11736480243338576 1105 96-97 0.04522903445945303 1105 98-99 -0.0017317603044020302 1105 100-101 0.13292221567092355 1106 1 0.15705595485427892 1106 2 0.0794816853953293 1106 3 0.11983279005300318 1106 4 0.1414291975743467 1106 5 0.06231816299626303 1106 6 0.02947103716940802 1106 7 0.15208735979247479 1106 8 0.1290187509038816 1106 9 0.044658800154564915 1106 10-11 0.09400010424366201 1106 12-13 -0.022904058701229246 1106 14-15 -0.020957647670797996 1106 16-17 -0.03726652865184832 1106 18-19 0.011000616565425503 1106 20-21 0.06891027812897477 1106 22-23 0.0944575077316756 1106 24-25 0.07259014485938309 1106 26-27 0.10260338858282836 1106 28-29 0.09418835492067501 1106 30-31 -0.04562059055337642 1106 32-33 -0.02522662043263324 1106 34-35 0.0050788514200093005 1106 36-37 -0.11707200626955938 1106 38-39 -0.0889312347296567 1106 40-41 -0.06551192117612459 1106 42-43 -0.10416228728589516 1106 44-45 -0.11601513262735352 1106 46-47 -0.18113747675033665 1106 48-49 -0.3224614924995137 1106 50-51 -0.2007552279185063 1106 52-53 -0.2610457937240014 1106 54-55 -0.4483598796880699 1106 56-57 -0.47129326558538764 1106 58-59 -0.36430473047498424 1106 60-61 -0.3092481143925383 1106 62-63 -0.2780993136352876 1106 64-65 -0.10938772415965303 1106 66-67 -0.13567637000794264 1106 68-69 -0.16137389217730203 1106 70-71 -0.12417452283985853 1106 72-73 -0.029682106593426738 1106 74-75 -0.15598796836367512 1106 76-77 -0.3631465682980206 1106 78-79 -0.28643625160595576 1106 80-81 -0.05750464840223657 1106 82-83 -0.11520421004861703 1106 84-85 -0.3403259659896136 1106 86-87 -0.31254718853925567 1106 88-89 -0.2703752540019124 1106 90-91 -0.2661464468973449 1106 92-93 -0.21578003646687094 1106 94-95 -0.28979000966510426 1106 96-97 -0.2965946046838468 1106 98-99 -0.17662972235049423 1106 100-101 -0.1283166863866647 1107 1 0.15978691877096196 1107 2 0.11108652679034492 1107 3 0.09804961000297396 1107 4 0.07117398925446139 1107 5 0.1380882148019822 1107 6 0.05911799942376916 1107 7 0.22580015428098932 1107 8 0.12273727577036908 1107 9 0.12891084386031793 1107 10-11 0.09047282428888082 1107 12-13 0.014205301174250451 1107 14-15 0.13037531052066953 1107 16-17 0.21877940729698508 1107 18-19 0.06180093354308269 1107 20-21 0.06626836738347919 1107 22-23 0.11493392929131829 1107 24-25 0.09584399285981249 1107 26-27 0.1296613557441546 1107 28-29 0.1304807487473525 1107 30-31 0.022867105912112606 1107 32-33 0.18642630967482177 1107 34-35 0.035889312715433164 1107 36-37 0.18141099231849012 1107 38-39 0.162828533370714 1107 40-41 0.10759324674718584 1107 42-43 0.1421252279757539 1107 44-45 0.18122370281529498 1107 46-47 0.2966876383089456 1107 48-49 0.15589987752545653 1107 50-51 0.31219491004937083 1107 52-53 0.3386324532965901 1107 54-55 0.41483121809230994 1107 56-57 0.38455997995098556 1107 58-59 0.33525826367331746 1107 60-61 0.4290743045497223 1107 62-63 0.41616049963699453 1107 64-65 0.23288039557943563 1107 66-67 0.34022700342943324 1107 68-69 0.4902972099395768 1107 70-71 0.3272656863447523 1107 72-73 0.5091624994947139 1107 74-75 0.3180569041096035 1107 76-77 0.22128422706295225 1107 78-79 0.3783172776099377 1107 80-81 0.500353455049158 1107 82-83 0.49029346811580865 1107 84-85 0.48028158313805847 1107 86-87 0.32007298806995976 1107 88-89 0.18379398316088214 1107 90-91 0.20200220220475984 1107 92-93 0.29020717601400037 1107 94-95 0.3046799174661743 1107 96-97 0.16459563612109207 1107 98-99 0.13416971700171487 1107 100-101 0.3577251969975599 1108 1 0.05271225701665827 1108 2 -1.9907137425434485E-4 1108 3 -0.077585637771314 1108 4 0.04875093323529711 1108 5 0.035397640469689406 1108 6 0.09168962863049757 1108 7 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-0.6025964234998078 2316 94-95 -0.7383235188154984 2316 96-97 -0.6545083035180497 2316 98-99 -0.7631166363012838 2316 100-101 -0.9694702927513923 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 0.0 12 1.0 13 22.0 14 110.0 15 396.0 16 1085.0 17 2519.0 18 3863.0 19 5243.0 20 7190.0 21 9525.0 22 10074.0 23 8464.0 24 6122.0 25 5237.0 26 5553.0 27 6303.0 28 7673.0 29 9014.0 30 11101.0 31 13435.0 32 16861.0 33 21885.0 34 29906.0 35 47278.0 36 77219.0 37 167410.0 38 346112.0 39 142561.0 40 85.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.82113095887904 25.56463208850711 14.2919524526913 20.322284499922542 2 13.688052494944014 24.088623670530623 42.73455508373293 19.488768750792435 3 15.930811152357633 32.016366047414564 33.44314700343783 18.609675796789976 4 9.00075040898329 20.205229581496287 46.028652735251136 24.765367274269288 5 8.347432262784645 43.45865099225979 38.20865307072605 9.985263674229513 6 22.490044146623323 45.828102526583585 19.970839118397752 11.71101420839534 7 22.73000307613006 36.255622251228374 27.184052344094244 13.830322328547318 8 22.229404477847705 38.22496903085275 22.76346638288674 16.7821601084128 9 25.60400229462675 16.56683100406548 25.807691987928266 32.021474713379504 10-11 21.52792211571237 29.087875474929543 32.39804083770504 16.986161571653046 12-13 22.581808431921914 28.822091602164928 30.733449173186123 17.86265079272703 14-15 20.74839334558243 30.31255975590492 29.608219502664596 19.330827395848054 16-17 17.660156217523966 33.05998037927853 30.56467771302201 18.715185690175506 18-19 17.027315203564985 32.11952635910909 33.95252575219694 16.90063268512899 20-21 18.000037412392647 30.99871342938619 33.8944845819373 17.10676457628387 22-23 18.465657101554257 30.399348596150915 33.0061591335539 18.128835168740927 24-25 19.111081550702107 30.878110424755366 32.281594765590576 17.729213258951955 26-27 18.22399215171141 30.316404918482554 34.116101046715606 17.34350188309043 28-29 17.717743314468542 30.153536069620067 34.83674741157032 17.29197320434107 30-31 18.49064782076995 30.455481194555162 33.135959361716175 17.91791162295872 32-33 17.427740783945918 31.103328405633686 33.69403401931416 17.774896791106237 34-35 18.087644765174883 30.607338232971127 33.57138700210341 17.733629999750583 36-37 18.52781195700069 29.675821617711858 33.183389313357885 18.612977111929567 38-39 17.567144852470467 32.05815964283636 33.28689693301519 17.087798571677986 40-41 16.950204105386554 31.292088941728117 34.59284924468536 17.164857708199964 42-43 17.07018357013992 31.232592845087755 32.553769922099086 19.14345366267324 44-45 18.595569957017318 30.172471130103673 32.75683607552315 18.47512283735586 46-47 18.764133570555614 30.470886923121693 32.71796875649521 18.04701074982749 48-49 17.610758572509752 30.61137609261554 32.76557522933491 19.0122901055398 50-51 17.433658027555357 30.040659532540097 32.10720729709729 20.418475142807253 52-53 17.984916570364394 31.01887455209052 31.97283860293812 19.02337027460696 54-55 18.538104437887963 30.35743792849371 31.409465651393575 19.694991982224753 56-57 19.065459216335082 30.587332995236157 30.753974027485636 19.593233760943125 58-59 19.150988102859138 30.007752679143007 30.860131691622115 19.981127526375737 60-61 19.000247337484723 32.01690208761151 30.777772466141784 18.20507810876198 62-63 17.977693900117224 31.3360485030886 31.93880371796044 18.74745387883373 64-65 17.952700343362626 31.149350271447695 31.317965846642448 19.579983538547236 66-67 18.858288092051115 30.4373196930521 30.205726590234534 20.498665624662248 68-69 18.357221838860667 30.65914400445623 30.75262302441782 20.231011132265277 70-71 18.781073070559774 29.70736234317972 32.24563729932408 19.265927286936424 72-73 19.214225438764228 29.44043531397311 31.514069138101615 19.831270109161046 74-75 17.63531854573873 31.29312817485721 30.669172604151946 20.402380675252118 76-77 17.327445731245998 31.037996441665765 31.5192133420906 20.115344484997628 78-79 17.777186567447252 31.016804980438963 30.765049839204067 20.440958612909714 80-81 18.46607488724196 30.201589915671175 30.30563028874062 21.026704908346254 82-83 18.69922374756674 29.85308469143732 31.03045457899556 20.417236982000382 84-85 17.492164182206665 30.10320624204987 31.279825990804866 21.124803584938604 86-87 16.98595372502723 31.136671627272804 31.306118588970826 20.571256058729144 88-89 18.231630515210213 30.023912754300348 30.272237510496254 21.472219219993182 90-91 17.745269410796386 29.938799561027928 31.541920585961208 20.77401044221448 92-93 18.882710070584714 30.75387010417273 30.429785252866203 19.933634572376352 94-95 18.037293920070503 29.598814442846333 31.100090621128857 21.263801015954307 96-97 18.312067159401735 29.332147221922 31.269849352765604 21.08593626591066 98-99 17.74100855496712 30.151062927644436 30.429057789675838 21.678870727712606 100-101 17.407934337093973 29.792007881544052 30.792529576574857 22.007528204787125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 584.0 1 399.5 2 459.5 3 817.0 4 627.5 5 451.0 6 861.5 7 1665.5 8 2334.0 9 2459.0 10 1903.5 11 1294.0 12 1223.5 13 1208.0 14 1195.0 15 1222.0 16 1266.0 17 1289.0 18 1295.0 19 1357.0 20 1508.5 21 1746.0 22 1955.0 23 2239.0 24 2508.0 25 2977.0 26 4546.0 27 7126.0 28 9038.0 29 13762.0 30 20424.0 31 25553.0 32 37014.5 33 49281.0 34 58336.5 35 64076.5 36 71826.0 37 77484.5 38 67281.0 39 57564.5 40 49653.5 41 39693.5 42 33502.0 43 31353.0 44 29224.0 45 26516.0 46 22943.5 47 20848.5 48 25686.0 49 22845.5 50 13995.0 51 8788.5 52 5533.0 53 4085.5 54 3075.5 55 2744.5 56 2716.5 57 2780.5 58 2787.5 59 2633.0 60 2731.5 61 2802.5 62 2110.0 63 1089.5 64 507.5 65 320.5 66 207.0 67 165.0 68 134.0 69 103.0 70 96.5 71 110.5 72 92.0 73 86.0 74 103.0 75 101.5 76 57.0 77 26.0 78 31.0 79 33.0 80 28.5 81 18.0 82 11.5 83 7.0 84 2.5 85 1.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044375254612116624 2 0.0022863128839966415 3 0.0012470797549072588 4 0.011015871168347454 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0019225812888153573 24-25 0.0 26-27 0.0 28-29 0.02353863037387451 30-31 0.020732700925333178 32-33 5.1961656454469125E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.03491823313740325 50-51 0.011639411045801082 52-53 0.0 54-55 0.0011431564419983207 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.637315951812838E-4 80-81 0.012626682518435996 82-83 0.006339322087445232 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 962248.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.139070181057793 #Duplication Level Percentage of deduplicated Percentage of total 1 71.61076772936691 10.841204383743142 2 10.38834964311737 3.145399086250411 3 4.2108973952522675 1.9124721357587255 4 2.270971465121679 1.3752158555862692 5 1.4346564655412393 1.0859682458768571 6 1.0787139315649457 0.979843554914789 7 0.8359588566534258 0.8858947859547132 8 0.6491036973017924 0.7861461142588743 9 0.5210813312857115 0.7099818159936071 >10 4.872774828544471 15.895274989694814 >50 1.0187776415381515 10.854166611375263 >100 0.9752461365241163 30.23156558788288 >500 0.09267997841697864 9.832250343265486 >1k 0.037212415576514155 8.862949901316968 >5k 0.0028084841944538983 2.6016665881272 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6493 0.674774070717736 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6310 0.6557561044554003 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5997 0.6232281075149027 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 5676 0.5898687240711334 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 4069 0.42286396022646966 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3733 0.38794572708906644 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 3357 0.34887056143530565 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2768 0.287659730131941 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 2647 0.2750850092699595 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 2646 0.27498108595705056 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 2290 0.23798438656146859 No Hit CTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT 2086 0.2167840307280452 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 2056 0.21366633134077703 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2053 0.21335456140205022 No Hit ATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTT 1963 0.20400146324024573 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 1812 0.18830904299099607 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 1767 0.18363249391009387 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1750 0.1818657975906419 No Hit CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG 1659 0.17240877611592853 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1571 0.16326352457994198 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1545 0.16056151844430958 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 1488 0.15463788960850008 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1486 0.1544300429826822 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 1477 0.15349473316650178 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 1467 0.15245550003741237 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 1466 0.15235157672450345 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1458 0.15152019022123195 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1399 0.1453887147596046 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1388 0.14424555831760627 No Hit ATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC 1359 0.14123178224324706 No Hit CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA 1323 0.13749054297852528 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1287 0.13374930371380353 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 1282 0.1332296871492588 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1257 0.13063160432653537 No Hit ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA 1241 0.12896883131999234 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1218 0.12657859512308678 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1196 0.12429228223909013 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 1195 0.12418835892618119 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 1189 0.12356481904872757 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1170 0.12159027610345774 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 1132 0.11764119021291808 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1130 0.11743334358710021 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 1130 0.11743334358710021 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1113 0.11566664726764826 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 1110 0.11535487732892144 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 1107 0.11504310739019463 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 1100 0.11431564419983205 No Hit TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC 1085 0.112756794506198 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 1083 0.1125489478803801 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 1054 0.1095351718060209 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 1052 0.10932732518020302 No Hit CTACTATACAATCAAAGATATCCTAGGTATCCTAATCATATTCTTAATTC 1049 0.10901555524147621 No Hit GATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGC 1049 0.10901555524147621 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 1032 0.10724885892202426 No Hit GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA 1019 0.10589785585420805 No Hit GGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCT 1009 0.10485862272511869 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1009 0.10485862272511869 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 999 0.1038193895960293 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 997 0.10361154297021141 No Hit CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT 989 0.10278015646693991 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 981 0.1019487699636684 No Hit ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC 977 0.10153307671203265 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 969 0.10070169020876116 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 965 0.10028599695712541 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.0392331290893824E-4 0.0 8 0.0 0.0 0.0 1.0392331290893824E-4 0.0 9 0.0 0.0 0.0 1.0392331290893824E-4 0.0 10-11 0.0 0.0 0.0 0.001402964724270666 0.0 12-13 0.0 0.0 0.0 0.0037932009211762456 0.0 14-15 0.0 0.0 0.0 0.006131475461627355 0.0 16-17 0.0 0.0 0.0 0.011223717794165328 0.0 18-19 0.0 0.0 0.0 0.016679691721884587 0.0 20-21 0.0 0.0 0.0 0.02785144785959545 0.0 22-23 0.0 0.0 0.0 0.056949975474098156 0.0 24-25 0.0 0.0 0.0 0.08552888652405616 0.0 26-27 0.0 0.0 0.0 0.09815556904249217 0.0 28-29 0.0 0.0 0.0 0.11192540800292647 0.0 30-31 0.0 0.0 0.0 0.13239830064598732 0.0 32-33 0.0 0.0 0.0 0.16627730065430119 0.0 34-35 0.0 0.0 0.0 0.21397810127950384 0.0 36-37 0.0 0.0 0.0 0.27451343104896037 0.0 38-39 0.0 0.0 0.0 0.36373159518128384 0.0 40-41 0.0 0.0 0.0 0.4856336412234684 0.0 42-43 0.0 0.0 0.0 0.5879981044387725 0.0 44-45 0.0 0.0 0.0 0.7011705921966063 0.0 46-47 0.0 0.0 0.0 0.8440651474463964 0.0 48-49 0.0 0.0 0.0 0.9858685079106426 0.0 50-51 0.0 0.0 0.0 1.1474692594840414 0.0 52-53 0.0 0.0 0.0 1.29639136688255 0.0 54-55 0.0 0.0 0.0 1.449574330110325 0.0 56-57 0.0 0.0 0.0 1.6349735203398708 0.0 58-59 0.0 0.0 0.0 1.8357013992234852 0.0 60-61 0.0 0.0 0.0 2.0540962413016186 0.0 62-63 0.0 0.0 0.0 2.2557074683449585 0.0 64-65 0.0 0.0 0.0 2.463865864101562 0.0 66-67 0.0 0.0 0.0 2.708189572750476 0.0 68-69 0.0 0.0 0.0 2.974389138766721 0.0 70-71 0.0 0.0 0.0 3.283197263075631 0.0 72-73 0.0 0.0 0.0 3.572415842901206 0.0 74-75 0.0 0.0 0.0 3.87498856843558 0.0 76-77 0.0 0.0 0.0 4.193045867593385 0.0 78-79 0.0 0.0 0.0 4.602607643767511 0.0 80-81 0.0 0.0 0.0 5.0611692619782005 0.0 82-83 0.0 0.0 0.0 5.504817884786458 0.0 84-85 0.0 0.0 0.0 5.894114614943341 0.0 86-87 0.0 0.0 0.0 6.2994155352882 0.0 88-89 0.0 0.0 0.0 6.770136181109236 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 150 0.0 47.495296 2 GACATAT 385 0.0 44.42735 1 ACATATA 380 0.0 43.745667 2 GGCAACA 45 9.6289837E-4 42.21804 8 GGATGAC 535 0.0 40.83708 8 ACAGTAC 60 8.294584E-5 39.57942 8 GTGTAGC 100 3.0227966E-8 38.010063 1 AGCGGGT 25 0.0016148102 38.006115 26-27 CATATAG 430 0.0 37.55442 3 GGGTATG 305 0.0 35.816124 7 ACCTGGG 200 0.0 35.621475 3 CTGGCTG 150 2.5465852E-11 34.829884 9 TGGGTAT 320 0.0 34.137245 6 GGATAGC 85 1.6330338E-5 33.526093 8 GCCTATC 85 1.6330338E-5 33.526093 7 CATTCCG 185 0.0 33.375072 9 GTATTAC 150 9.513315E-10 31.675053 1 CATCGCC 30 0.003951513 31.666824 80-81 GACTGTG 60 0.0039525502 31.663534 7 GGGATGC 135 1.1979864E-8 31.663532 7 >>END_MODULE