FastQCFastQC Report
Fri 10 Feb 2017
SRR2031640_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031640_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541764
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19920.12920265358381697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17500.11350634727493962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19500.040.515021
GTATCAA31250.033.657731
ATCAACG33950.030.2268983
TCAACGC33950.029.947024
CAACGCA34700.029.4366665
AACGCAG35900.028.7108556
GTACATG39000.027.9455991
TATCAAC37650.027.5409932
TACATGG40150.026.655392
ACGCAGA41150.024.9316277
ACATGGG41850.024.1804183
CGCAGAG42250.024.0576828
CCTATAC4700.022.2384473
GAGTACA30450.021.7255131
AGTACAT27800.021.3871692
GAGTACT27050.020.71960412-13
CATGGGG27750.020.7158154
AATACCG1150.003719571720.6562655
GCAGAGT49600.020.2054029
GTATAAT7850.020.0072231
Produced by FastQC (version 0.11.5)