FastQCFastQC Report
Fri 10 Feb 2017
SRR2031640_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031640_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541764
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21580.1399695413824684No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20180.13088903360047324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20900.041.1542051
GTATCAA31200.033.660431
TCAACGC33900.030.2638854
ATCAACG35150.029.1876433
CAACGCA35300.029.0617335
AACGCAG37350.027.5938036
GTACATG35850.027.4386751
TATCAAC39450.026.2470282
TACATGG36700.025.754772
ACGCAGA40250.025.605687
CGCAGAG41800.024.6561858
ACATGGG36750.024.5565243
GAGTACT29600.020.5381212-13
GAGTACA26400.020.520261
GCAGAGT51050.019.9095069
CGATACT1200.004765682419.7906194
GTATAGC5350.019.5411831
CATGGGG24600.019.1148414
CCGTGCG1507.728812E-418.997769
GTACTTT32100.018.71664214-15
Produced by FastQC (version 0.11.5)