FastQCFastQC Report
Fri 10 Feb 2017
SRR2031638_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031638_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1858680
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT57610.3099511481266275No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT29260.15742354789420449No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20680.11126175565455054No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG550.002576136534.5561038
GTACATG49950.029.2773781
TACATGG54350.025.918562
ACATGGG51450.025.4985263
GGACCGC2309.006362E-820.6568686
CATGGGG25850.020.4105054
CATGGGA27700.020.0769394
GAGTACT26800.016.48695612-13
AGAGTAC51900.016.43240510-11
GTATAGC7550.016.4042241
GTATTAC5250.016.3321191
ACACCGT2951.228389E-616.1053546
GAGTACA42800.016.0268441
AGTACAT37550.015.84231952
CTGATCG2406.1343846E-515.8382129
GTATAGA10350.015.6483261
ACCGTGT3654.1800376E-815.621258
GTATAGG7950.015.5788551
GTATATA14150.015.4856961
TGCACCG2154.7645194E-415.4757235
Produced by FastQC (version 0.11.5)