FastQCFastQC Report
Fri 10 Feb 2017
SRR2031638_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031638_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1858680
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT61580.3313103923214324No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27430.1475778509479846No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18800.10114705059504595No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG49150.025.3297821
ACATGGG48150.023.97373
TATCCCG1252.2575783E-422.7985885
CCGCGCA1050.002197519622.6176459
TACATGG53550.022.2658542
CATGGGA26150.021.979974
GTATAGG8100.018.772351
TGCACCG2051.591104E-518.5354355
CATGGGG22850.018.5019384
TAATACT14350.018.2064064
TAGGTCG1650.001464880917.2716565
GAGTACT25950.017.205112-13
ATGGGAT14850.016.951815
TTAGTAC6400.016.329313
TACACTG18100.016.0072945
TAGACAG12200.015.9621255
GAGTACA41700.015.953121
AGAGTAC51200.015.67773810-11
TAGAACA13100.015.2297014
CCTACAC9050.014.6971983
Produced by FastQC (version 0.11.5)