FastQCFastQC Report
Fri 10 Feb 2017
SRR2031636_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031636_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2738401
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC38890.14201718448101647No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC32850.11996051710468994No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31630.11550536243596173No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATCG801.8343253E-623.7904660-61
CGATACG701.8099052E-523.7007628-29
GTACATG76650.022.621421
ACATGGG71050.021.8747543
TAATACT32250.020.0440084
TACATGG88100.018.6899742
GTCACGC650.00755354318.2747310-11
TCGTACG650.007554739218.2742388-89
GTATTAT17350.018.1207031
AATACCG3259.957148E-917.5498455
GTATAGG15500.017.5175671
CGTCCGA3050.017.13630510-11
GTATTAC11200.017.0126741
GTAATAC10150.016.8576093
GGTATCA75100.016.8087941
CATGGGG31450.016.7755874
ACCTGGG14650.016.5459633
CTCGTCC6100.016.357989
TCGTCCG3050.016.35738210-11
GTATATA31850.016.3022561
Produced by FastQC (version 0.11.5)