FastQCFastQC Report
Fri 10 Feb 2017
SRR2031636_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031636_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2738401
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC42400.15483488356891484No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34170.12478084838560898No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGC1201.7130084E-423.7472386
ACATGGG66000.022.8146573
GTACATG75850.021.9927851
TATAGCG2056.843984E-720.8512335
TACATGG83300.019.3313082
GTATTAC9600.019.307321
GGACCGT2001.287084E-518.9977916
CGTACGA757.7321776E-418.9977912-13
GTTACAC17500.018.4573573
TAATACT32250.018.4107284
GTATAGG15750.018.4067841
TACACTG41350.018.3775485
GGTATCA74900.018.274241
ATCTCGC4950.018.2302048
ACCTGGG15100.017.6160963
CCGCAAT2300.017.55230732-33
TAGCGCT1650.001465653517.2707187
CATGGGT17400.017.198164
CATGGGG28250.016.9821854
GTATCAA92100.016.8739511
Produced by FastQC (version 0.11.5)