FastQCFastQC Report
Fri 10 Feb 2017
SRR2031635_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031635_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473341
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC54701.1556150851077764No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49821.052518163438198No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC24100.5091466828354189No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA23030.48654141517426125No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT23010.48611888680676296No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC21170.4472462769969219No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG20600.4352042185232211No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19950.4214720465795272No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT18830.3978104579996239No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18150.3834444935046827No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16310.34457188369484154No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA15410.3255581071574193No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT15200.3211215592986874No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGAC14640.30929076500873576No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG14230.3006289334750212No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14200.2999951409237738No Hit
AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA13570.28668549734757814No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC12560.2653478147889154No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT12290.2596436818276887No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG12260.2590098892764413No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC12160.2568972474389499No Hit
TTTGTAAGGTGGCTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGT12080.25520713396895683No Hit
CTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGAATAAAAA11930.25203817121271976No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11810.24950300100773015No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC11600.2450664531489983No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC11570.24443266059775087No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC11500.24295381131150692No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA11490.24274254712775778No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC10960.23154554538905356No Hit
ACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTTGATTCATAAA10900.23027796028655872No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10880.22985543191906044No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT10560.22309497803908812No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC10290.2173908450778614No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA10280.21717958089411227No Hit
CTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGTAAATAGATAGAAAC10210.2157007316078683No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA10120.2137993539541261No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG9980.21084165538163818No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT9970.21063039119788904No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT9800.2070389000741537No Hit
GATGAAAGACTAATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACC9670.20429246568541495No Hit
GATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGA9620.20323614476666926No Hit
GTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGAATAAAAAATCCT9610.20302488058292012No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC9500.20070097456167962No Hit
GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCT9430.19922212527543567No Hit
AAACTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGAATAA9190.1941517848654564No Hit
GTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATATATCTGGGTCAAT9160.193517992314209No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT9030.19077155792547024No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA9020.1905602937417211No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8910.1882363877204806No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGT8880.1876025951692332No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCA8660.18295478312675217No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT8580.18126466965675908No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC8500.179574556186766No Hit
CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC8430.17809570690052204No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA8420.1778844427167729No Hit
AACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGACCCAGA8310.1755605366955324No Hit
GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT8200.1732366306742919No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA8200.1732366306742919No Hit
TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA7840.16563112005932298No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA7840.16563112005932298No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7820.1652085916918247No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC7760.1639410065893299No Hit
AACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAACAAG7690.16246215730308594No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT7670.16203962893558765No Hit
ACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAG7620.160983308016842No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA7610.16077204383309285No Hit
ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT7600.1605607796493437No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG7440.15718055270935752No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC7430.15696928852560837No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC7420.15675802434185923No Hit
CCCTAATTGTCGATATGAACTCTTAAATAGGATTGCGCTGTTATCCCTAG7300.1542228541368696No Hit
AATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACCTTTTGCATAAT7160.1512651555643817No Hit
ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA7150.15105389138063255No Hit
CTCTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGAT7100.1499975704618869No Hit
TTTCACCTCTACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTT6990.1476736644406464No Hit
ACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTC6980.14746240025689725No Hit
TTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGT6960.147039871889399No Hit
GTACATGGGATGGCCCAAAAACTATAGTATAAGTTTGAAATTTCGGTTGG6890.145561022603155No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGATATGAACTCT6870.14513849423565675No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT6820.14408217331691106No Hit
GTACAAGGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCT6730.14218079566316882No Hit
GGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTA6630.14006815382567747No Hit
AGGTTAGAGGGTGTACGTATATATTTTATTTAGATTTTATTCATAAATTA6590.13922309709068093No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC6590.13922309709068093No Hit
GTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACCGC6530.13795551198818612No Hit
GATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGT6490.13711045525318957No Hit
GTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTAT6480.13689919106944043No Hit
GTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG6470.13668792688569129No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT6400.13520907759944734No Hit
AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCGACAATTAGGGTT6250.1320401148432103No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCTCGATGTTGGATC6230.13161758647571203No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC6220.1314063222919629No Hit
GTCGTAAACCCTAATTGTCGATATGAACTCTTAAATAGGATTGCGCTGTT6160.13013873718946806No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5980.1263359818819836No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA5970.12612471769823447No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC5960.12591345351448532No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT5950.1257021893307362No Hit
GTTTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTT5930.12527966096323792No Hit
AGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAAT5860.12380081167699397No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA5840.12337828330949568No Hit
ATTAAGTTGAGAGCGCTTATTTGTAAGGTGGCTCTATTTCTCTTGTCCTT5800.12253322657449915No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACC5750.12147690565575346No Hit
GATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTG5750.12147690565575346No Hit
CTATTTTGCCACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCT5730.12105437728825519No Hit
ACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTAT5710.12063184892075693No Hit
GGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAG5700.12042058473700779No Hit
GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACC5610.11851920708326555No Hit
GGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGG5590.11809667871576729No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT5530.11682909361327246No Hit
GGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA5500.11619530106202504No Hit
CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA5470.11556150851077764No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT5450.11513898014327936No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA5430.1147164517757811No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT5410.11429392340828282No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT5380.1136601308570354No Hit
GGGTAACTTGGTCCGTTGATCAAAATATATCTGGGTCAATAAGATATGTT5350.113026338305788No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT5280.11154748901954406No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC5210.1100686397333001No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA5170.10922358299830354No Hit
CTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAAGG5170.10922358299830354No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA5160.10901231881455442No Hit
CGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCACTATTTTGCCACAT5140.10858979044705613No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG5110.10795599789580873No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA5110.10795599789580873No Hit
CCATTAATAGCTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTC5100.1077447337120596No Hit
GTACATGGGTAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG5000.10563209187456823No Hit
GTTTTTAGGTAGCTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTA4960.10478703513957167No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG4900.10351945003707685No Hit
GACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTT4890.10330818585332774No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT4880.1030969216695786No Hit
GGTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTT4870.10288565748582945No Hit
AGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGTTCTTA4860.10267439330208031No Hit
CTGTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATATATCTGGGTCA4850.10246312911833119No Hit
TAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGAATAAAAAATC4810.10161807238333463No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC4790.10119554401583637No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTC4760.10056175146458896No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG4750.10035048728083981No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG505.5697456E-966.489875
CATGGAT659.094947E-1265.773114
ATGGATG659.094947E-1265.759215
TGGATGG659.094947E-1265.759216
GGCATCT452.047218E-763.3236858
GGCTCAG301.3014185E-463.3236857
TACCTGG656.693881E-1058.4649852
ATGGCTC352.788361E-454.2774475
GTTATAT2650.050.228791
GGGAACC601.4919751E-647.4927649
GGGCATC601.4919751E-647.4927647
GCTCAGC405.3889956E-447.4927648
TAACTTT13300.047.1356746
ACATGGC857.203198E-944.7085153
TGGGAAC751.3621502E-744.326588
AATGTTC751.3621502E-744.326588
GCGGATA652.588844E-643.839479
TCTCTAG554.9355396E-543.2163281
CTCTAGG1103.274181E-1143.2163281
AGGCTTA1006.366463E-1042.7434846
Produced by FastQC (version 0.11.5)