FastQCFastQC Report
Fri 10 Feb 2017
SRR2031634_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031634_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3967
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC100.25207965717166625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.20166372573733302No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT60.15124779430299976No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.12603982858583312No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.12603982858583312No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.10083186286866651No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers