FastQCFastQC Report
Fri 10 Feb 2017
SRR2031633_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031633_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2916394
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC40230.1379443243951263No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC35960.12330295563630977No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32450.037.440881
GTATCAA51300.029.7203031
TCAACGC51700.028.2266714
ATCAACG52600.027.7437063
AACGCAG55200.026.6842736
CAACGCA55250.026.4990485
CGCAGAG60850.023.5046398
ACGCAGA63050.023.060527
TATCAAC65250.022.7582362
GTATAGG19000.022.3180891
GTACATG76250.021.9324861
CCTCGTC7650.021.7397378
ACATGGG67650.021.431073
CGTCCGA4350.019.66180810-11
TAATACT30800.019.4460624
CCGCAAT2950.019.29011532-33
GCCTCGT8400.019.2323517
CTCGTCC8950.019.1129259
GCAGAGT72700.018.5622929
AGCCTCG8800.018.3581526
Produced by FastQC (version 0.11.5)