FastQCFastQC Report
Fri 10 Feb 2017
SRR2031633_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031633_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2916394
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC42580.1460022205504469No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC38790.13300671994250435No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38350.033.5833131
GTATCAA56350.027.8316961
TCAACGC57050.026.227154
ATCAACG58000.025.798013
AACGCAG60600.025.002136
CAACGCA60600.024.7675
CCTCGTC8250.022.4527558
CGCAGAG67900.021.8244748
GTACATG75600.021.7507361
AGCCTCG9250.021.5658476
ACGCAGA70000.021.5089997
TATCAAC71450.021.3409582
CTCGTCC9250.021.0523749
ACATGGG67250.020.978183
GCCTCGT9800.020.3555187
AGCCCGT2851.9699655E-919.9984046
CATGGGG25450.019.970654
CGTCCGA5100.019.09161410-11
TTGGTCG1759.949527E-518.9984869
CGTTATA1559.626754E-418.3878362
Produced by FastQC (version 0.11.5)