FastQCFastQC Report
Fri 10 Feb 2017
SRR2031632_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031632_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856544
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73870.8624192102215414No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65350.7629497142003213No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39060.4560186049986924No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT25600.29887548100272726No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT23410.27330761758882205No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG20380.23793290245451487No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA19220.2243901072215788No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC19210.2242733589868121No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA18310.21376601785780996No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC17420.20337542496357458No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT17260.2015074532073075No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC16680.19473605559083945No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA16290.19018287443493853No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT16130.1883149026786715No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC15060.17582284155863562No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG14800.17278738745470168No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT14600.17045242275936787No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG14520.16951843688123436No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG14470.1689346957074009No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG14450.16870119923786753No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT14020.16368102514289984No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13950.162863787499533No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA13690.15982833339559904No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12660.14780326521462997No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT12620.1473362722755632No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG11800.1377629170246946No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11730.13694567938132776No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA11410.13320973586879367No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC11230.13110826764299324No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC10960.1279560653042926No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC10930.12760582059999254No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG10650.12433687002652519No Hit
TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC10630.12410337355699183No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA10470.12223540180072477No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG10450.12200190533119139No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT10360.12095117121829117No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC10360.12095117121829117No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG10280.12001718534015765No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT10130.11826596181865731No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC9710.11336253595845631No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC9690.11312903948892293No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA9590.11196155714125602No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9550.11149456420218927No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA9430.11009358538498898No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT9380.10950984421115553No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA9190.10729162775058841No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT9160.10694138304628834No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT9070.10589064893338812No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT9050.10565715246385475No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8950.10448967011618783No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA8850.10332218776852094No Hit
ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT8780.1025049501251541No Hit
ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA8580.1001699854298203No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG353.5235644E-881.648331
CCGTCCA301.2980151E-463.363099
ACCGTCC407.522847E-659.40298
GAACCGT352.783491E-454.301696
CATTCCG1100.047.5223169
AGCGCTC205.432511E-447.42244732-33
TACCTGG2150.046.4742432
ACCTGGG2100.045.264653
AGAACCG459.595938E-442.2470055
CCGACCT608.2660576E-539.6019339
GAAACCG756.8923946E-638.0223055
GGTATCA40750.037.86881
GGATGCC2700.036.9618038
GGATGAC7900.036.09298
GATGACC6650.035.7310649
GTATGGT958.5149804E-735.0944631
ATGGGAT16550.034.1741265
TGACGGC352.0735593E-433.87317732-33
GGGATGC2950.033.8215337
GTACATG61150.033.2579571
Produced by FastQC (version 0.11.5)