##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031632_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 856544 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.216535285986474 33.0 31.0 34.0 31.0 34.0 2 32.40710109463145 34.0 31.0 34.0 31.0 34.0 3 32.5226923543916 34.0 31.0 34.0 31.0 34.0 4 35.6548490211828 37.0 35.0 37.0 33.0 37.0 5 35.46987195053611 37.0 35.0 37.0 33.0 37.0 6 35.70607697911608 37.0 36.0 37.0 33.0 37.0 7 35.817466469906975 37.0 36.0 37.0 35.0 37.0 8 35.869987998281466 37.0 37.0 37.0 35.0 37.0 9 37.774806664923226 39.0 39.0 39.0 35.0 39.0 10-11 37.73728553349274 39.0 38.5 39.0 35.0 39.0 12-13 37.79262127806628 39.0 39.0 39.0 35.0 39.0 14-15 39.177107655882246 41.0 39.0 41.0 36.0 41.0 16-17 39.157050892890496 41.0 39.0 41.0 36.0 41.0 18-19 39.19406008331154 41.0 39.0 41.0 36.0 41.0 20-21 39.2607706083984 41.0 39.0 41.0 36.0 41.0 22-23 39.175380365748865 41.0 39.0 41.0 36.0 41.0 24-25 39.112187464975534 41.0 39.0 41.0 35.5 41.0 26-27 38.994607982777296 41.0 39.0 41.0 35.0 41.0 28-29 38.88568129599881 41.0 39.0 41.0 35.0 41.0 30-31 38.687194119624905 40.0 38.0 41.0 35.0 41.0 32-33 38.462743303321254 40.0 38.0 41.0 35.0 41.0 34-35 38.3334434658348 40.0 38.0 41.0 35.0 41.0 36-37 38.1805552312549 40.0 38.0 41.0 34.0 41.0 38-39 37.98374981320282 40.0 38.0 41.0 34.0 41.0 40-41 37.85185174375164 40.0 37.0 41.0 33.0 41.0 42-43 37.66445506593941 40.0 37.0 41.0 33.0 41.0 44-45 37.406238325176524 40.0 37.0 41.0 32.5 41.0 46-47 37.07330213042179 40.0 37.0 41.0 31.0 41.0 48-49 37.028959983375046 40.0 37.0 41.0 31.0 41.0 50-51 36.76635467646729 39.5 37.0 40.5 30.5 41.0 52-53 36.899284216572646 40.0 37.5 40.5 31.0 41.0 54-55 37.18337178241865 40.0 38.0 41.0 31.0 41.0 56-57 37.11219388612844 40.0 38.0 41.0 31.0 41.0 58-59 36.957871983225616 40.0 37.0 41.0 30.0 41.0 60-61 36.74022525404416 40.0 37.0 41.0 29.5 41.0 62-63 36.440292617775626 40.0 36.0 41.0 28.0 41.0 64-65 36.20528484234318 39.0 36.0 41.0 28.0 41.0 66-67 35.9792596760937 39.0 35.5 41.0 28.0 41.0 68-69 35.69070473904435 39.0 35.0 41.0 28.0 41.0 70-71 35.36510733832704 38.0 35.0 40.5 26.5 41.0 72-73 34.966590741397994 37.0 35.0 39.5 26.0 41.0 74-75 34.485723442111556 37.0 35.0 39.0 26.0 41.0 76-77 34.04066983132215 36.0 34.5 39.0 25.0 41.0 78-79 33.62814928363283 36.0 34.0 38.0 24.0 39.5 80-81 33.23997424533941 35.5 34.0 37.0 23.5 39.0 82-83 32.846934891844434 35.0 34.0 37.0 22.5 39.0 84-85 32.461307300033624 35.0 34.0 36.0 20.5 38.0 86-87 32.19538284081145 35.0 34.0 36.0 20.0 37.0 88-89 31.892093692793363 35.0 33.5 36.0 19.5 37.0 90-91 31.653121731049424 35.0 33.0 35.0 19.0 36.5 92-93 31.46128686909254 35.0 33.0 35.0 18.0 36.0 94-95 31.27755316714611 35.0 33.0 35.0 17.5 36.0 96-97 31.131741626816606 35.0 33.0 35.0 16.0 36.0 98-99 30.988857548473867 35.0 32.0 35.0 15.0 35.5 100-101 30.568650880748685 34.5 31.5 35.0 12.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15370096912167241 1101 2 0.175080071021938 1101 3 0.2736217871879276 1101 4 0.2964749864367775 1101 5 0.2896017957518424 1101 6 0.2769277423855314 1101 7 0.2003999061662114 1101 8 0.29648725547269805 1101 9 0.14978859405957934 1101 10-11 0.27364556136041074 1101 12-13 0.35061350872010877 1101 14-15 0.3542347106117134 1101 16-17 0.3788227421261823 1101 18-19 0.2571935997040171 1101 20-21 0.17321405244393162 1101 22-23 0.2916372380265386 1101 24-25 0.37622271724445966 1101 26-27 0.16474166537349788 1101 28-29 0.23576282492266643 1101 30-31 0.3334088676351641 1101 32-33 0.17415266377288674 1101 34-35 0.19806632590511697 1101 36-37 0.20655091704929163 1101 38-39 0.1634885176667069 1101 40-41 0.20984699377741833 1101 42-43 0.28468655110274455 1101 44-45 0.29523879710872336 1101 46-47 0.3118929244261963 1101 48-49 0.27602411168470553 1101 50-51 0.18814568971413337 1101 52-53 0.43478340726002074 1101 54-55 0.36040554245581546 1101 56-57 0.26312429417995276 1101 58-59 0.3091934221660182 1101 60-61 0.2010902594662909 1101 62-63 0.11056435299388312 1101 64-65 0.1670920439109338 1101 66-67 0.2561301138398733 1101 68-69 0.22979050616419983 1101 70-71 0.42255651176235176 1101 72-73 0.5532807886773412 1101 74-75 0.3951642810915317 1101 76-77 0.4130291030024793 1101 78-79 0.4903555993437365 1101 80-81 0.10900523049273403 1101 82-83 0.4024419067681251 1101 84-85 0.2320168333776209 1101 86-87 0.33329317419619287 1101 88-89 0.4293804345373964 1101 90-91 0.19291431519679492 1101 92-93 0.24471258966730502 1101 94-95 0.12564528406660358 1101 96-97 0.05390705981230326 1101 98-99 -0.09004280348401394 1101 100-101 0.17291704591059087 1102 1 0.1484841653567699 1102 2 0.12048433403055725 1102 3 0.21599339017659247 1102 4 0.2267709457708449 1102 5 0.32129544995003556 1102 6 0.1651907195777227 1102 7 0.1704661375754526 1102 8 0.22510975215326567 1102 9 0.30027452675966515 1102 10-11 0.2928196721860701 1102 12-13 0.38703292717219995 1102 14-15 0.33625480787681994 1102 16-17 0.3389581904649006 1102 18-19 0.5030865452339768 1102 20-21 0.4001803710617935 1102 22-23 0.1646269222684893 1102 24-25 0.3455622101774196 1102 26-27 0.44290179925050666 1102 28-29 0.31212536135780056 1102 30-31 0.31800986857010827 1102 32-33 0.3459497112505119 1102 34-35 0.21137546081135383 1102 36-37 0.33893516921576605 1102 38-39 0.48567721825831 1102 40-41 0.6938823171956869 1102 42-43 0.6134708944061416 1102 44-45 0.35564451359428517 1102 46-47 -0.3031399555794607 1102 48-49 0.56316830445504 1102 50-51 0.5852473781043841 1102 52-53 0.3641994237903816 1102 54-55 0.44740595885731693 1102 56-57 0.38382573430063616 1102 58-59 0.51396868320262 1102 60-61 0.20045387226003442 1102 62-63 -0.07905939289120312 1102 64-65 0.08770863240410876 1102 66-67 0.2804542470569231 1102 68-69 0.5345767664858485 1102 70-71 0.5244845292983555 1102 72-73 0.5493014044801754 1102 74-75 0.4669149744393266 1102 76-77 0.5549473783145871 1102 78-79 0.657627226687957 1102 80-81 0.5629967924697681 1102 82-83 0.46321295664987616 1102 84-85 0.4876087913289311 1102 86-87 0.44628332983607777 1102 88-89 0.5428459164239321 1102 90-91 0.26723019895006317 1102 92-93 0.284031105392355 1102 94-95 0.5396190900549271 1102 96-97 0.4825688396537551 1102 98-99 0.18688776745744562 1102 100-101 0.27752810333471345 1103 1 -0.06970616561551424 1103 2 -0.06741798921011366 1103 3 -0.2342906616112188 1103 4 0.030320779395871966 1103 5 -0.17033071836786462 1103 6 -0.087423833788705 1103 7 0.07450445514760418 1103 8 -0.0031031377137580307 1103 9 0.13181641845193326 1103 10-11 0.03926745998724357 1103 12-13 0.0528095064826104 1103 14-15 0.10971568284144695 1103 16-17 0.1314075956962597 1103 18-19 0.0405440751727113 1103 20-21 -0.04625039115844487 1103 22-23 0.005156067308917045 1103 24-25 0.18214667834083542 1103 26-27 -0.04619682142705983 1103 28-29 -0.021116584666096117 1103 30-31 0.13470445218198535 1103 32-33 0.16962353116493034 1103 34-35 0.11003694969840438 1103 36-37 0.15562975282764313 1103 38-39 0.11180046844170732 1103 40-41 -0.121591682488841 1103 42-43 -6.668475511446559E-4 1103 44-45 -0.0496519443337462 1103 46-47 0.15983316152900073 1103 48-49 0.0392461113599083 1103 50-51 0.20336610148626733 1103 52-53 0.2121972905852374 1103 54-55 -0.021124257793196932 1103 56-57 0.05756302457927376 1103 58-59 0.31346993383169064 1103 60-61 0.30843611558062634 1103 62-63 0.3033051097740511 1103 64-65 0.5688315195420373 1103 66-67 0.41556099494562915 1103 68-69 0.23195582852657282 1103 70-71 0.23848606660816785 1103 72-73 0.27446846799310265 1103 74-75 0.09337247683667727 1103 76-77 -0.03328894676384664 1103 78-79 0.29941025568903257 1103 80-81 0.38419063622000493 1103 82-83 0.05366260724991179 1103 84-85 0.02733973707491799 1103 86-87 0.22564056408052835 1103 88-89 0.19599477258030973 1103 90-91 0.17326401475831332 1103 92-93 0.16458664008322543 1103 94-95 0.1466398888050513 1103 96-97 0.5423586738796793 1103 98-99 0.399735070530312 1103 100-101 0.35672584794599516 1104 1 -0.08575665702471014 1104 2 0.18429411652569883 1104 3 0.1927246188039362 1104 4 0.059620238514419555 1104 5 0.01125874644056779 1104 6 0.07713711991760164 1104 7 0.0689151183043677 1104 8 0.2669879689338046 1104 9 0.37327759815391204 1104 10-11 0.428079417420534 1104 12-13 0.3202179142528436 1104 14-15 0.3401375795141277 1104 16-17 0.2585238772105427 1104 18-19 0.5033233420692653 1104 20-21 0.41363252068953216 1104 22-23 0.4848633012270014 1104 24-25 0.38839457891187834 1104 26-27 0.4336806519001897 1104 28-29 0.5346133996353117 1104 30-31 0.5063747864224979 1104 32-33 0.40356447065796885 1104 34-35 0.488208372785607 1104 36-37 0.6481152253505442 1104 38-39 0.4588112243732425 1104 40-41 0.48174021123606536 1104 42-43 0.5250655817995025 1104 44-45 0.6496138553687345 1104 46-47 0.7121195110704974 1104 48-49 0.5494410526216029 1104 50-51 0.43088021764667417 1104 52-53 0.4035703799620691 1104 54-55 0.43107045952940126 1104 56-57 0.48575284266303953 1104 58-59 0.45529878495561604 1104 60-61 0.48678303379029586 1104 62-63 0.37202813139789015 1104 64-65 0.6479002985484286 1104 66-67 0.6310772316720943 1104 68-69 0.27458080596127843 1104 70-71 0.2477943918865435 1104 72-73 0.38276358315035 1104 74-75 0.2868866981113527 1104 76-77 0.24455547955219714 1104 78-79 0.22370399065027158 1104 80-81 0.452520621892468 1104 82-83 0.26699543786283897 1104 84-85 0.3207262216653888 1104 86-87 0.33886462427901876 1104 88-89 0.33372645242053167 1104 90-91 0.5741606851496819 1104 92-93 0.286917937398929 1104 94-95 0.28202592094716294 1104 96-97 0.23659612700671673 1104 98-99 0.2887835350622474 1104 100-101 0.30037203332642903 1105 1 0.1979393541308454 1105 2 0.1057281923394271 1105 3 0.15278724647509279 1105 4 0.23866565580797072 1105 5 0.2161931219374864 1105 6 0.1784055748978446 1105 7 0.27815514619325654 1105 8 0.2756237376517774 1105 9 0.20730815360753496 1105 10-11 0.2622720464372037 1105 12-13 0.15050242980579043 1105 14-15 0.21649685684951692 1105 16-17 0.2315707378762042 1105 18-19 0.2356298017710472 1105 20-21 0.04559403892127989 1105 22-23 0.11137547815017257 1105 24-25 0.1175054372290063 1105 26-27 0.019952715310708413 1105 28-29 0.09973945005548046 1105 30-31 0.05518033484273843 1105 32-33 0.0543081742529381 1105 34-35 0.10401146258048044 1105 36-37 0.08941095761724682 1105 38-39 0.16370584597733995 1105 40-41 0.25365072216533235 1105 42-43 0.2767902891496945 1105 44-45 0.25324613797610596 1105 46-47 0.34553051739442253 1105 48-49 0.2282601785252396 1105 50-51 0.5795781247571199 1105 52-53 0.615697152068293 1105 54-55 0.6035717744658555 1105 56-57 0.5186782398961682 1105 58-59 0.2902375639606589 1105 60-61 0.3056734720616774 1105 62-63 0.3998283344485358 1105 64-65 0.3549844422495809 1105 66-67 0.2224683732815791 1105 68-69 0.14179668794726297 1105 70-71 0.13691466881827807 1105 72-73 0.3156926499735846 1105 74-75 0.36063322729070535 1105 76-77 0.1293915100342602 1105 78-79 0.14284763063901806 1105 80-81 0.07920710434571987 1105 82-83 0.05384729651022013 1105 84-85 0.24017871882146125 1105 86-87 0.06626912319648426 1105 88-89 0.3292403785149851 1105 90-91 0.4041332855435549 1105 92-93 0.10216936548107824 1105 94-95 0.11062548304274245 1105 96-97 0.23655943110565048 1105 98-99 0.220471057232686 1105 100-101 0.26668213815438335 1106 1 0.13541637470903822 1106 2 0.07478699312631676 1106 3 0.06348115868138393 1106 4 0.20328776987687291 1106 5 0.11228633594839721 1106 6 0.015186295305085196 1106 7 0.05215892903250818 1106 8 0.10689318724487151 1106 9 0.08830222918093256 1106 10-11 0.22604984443055542 1106 12-13 0.1801539385825066 1106 14-15 0.04664623730535311 1106 16-17 0.21927082467683334 1106 18-19 0.2107144228996063 1106 20-21 0.27823770531882985 1106 22-23 0.2617461812351962 1106 24-25 0.25277226385139784 1106 26-27 0.05301654977548509 1106 28-29 0.1712845769018898 1106 30-31 0.12102182705532272 1106 32-33 0.1222940966327144 1106 34-35 0.06957644364732829 1106 36-37 0.06684563920300945 1106 38-39 0.23224516969059295 1106 40-41 0.042708158400984075 1106 42-43 -0.20642667828681027 1106 44-45 0.0802087227393713 1106 46-47 0.24819347703023453 1106 48-49 9.619520628021405E-4 1106 50-51 -0.1910635554603033 1106 52-53 -0.1408669079264726 1106 54-55 0.12956837066861482 1106 56-57 -0.017782758576288415 1106 58-59 0.068044085757478 1106 60-61 0.23440315067195883 1106 62-63 0.20760002228470142 1106 64-65 0.2623001425486464 1106 66-67 0.371124858879881 1106 68-69 0.3185837762602617 1106 70-71 0.2864230513323136 1106 72-73 0.33148259247290923 1106 74-75 0.3809858879760881 1106 76-77 0.35993623130571706 1106 78-79 0.3419801930009001 1106 80-81 0.4396210940281904 1106 82-83 0.409767743173326 1106 84-85 0.4319341760787907 1106 86-87 0.32185335959740513 1106 88-89 0.33665538782047477 1106 90-91 0.3891620737841528 1106 92-93 0.4456487057963656 1106 94-95 0.4173311152279595 1106 96-97 0.5719661242294478 1106 98-99 0.5393069813181519 1106 100-101 0.5699887111218693 1107 1 -0.08911459788474474 1107 2 -0.046080357167326724 1107 3 -0.07477334297808369 1107 4 0.25662936302999384 1107 5 -0.019588147091859298 1107 6 0.007659015404485103 1107 7 0.03238727382691309 1107 8 0.15060851417107557 1107 9 0.20146233702003258 1107 10-11 0.15701690090072873 1107 12-13 0.16682200111257117 1107 14-15 -0.09914679191433606 1107 16-17 0.08320269500688227 1107 18-19 0.15728977010621037 1107 20-21 0.07430552351511466 1107 22-23 0.06132067361966875 1107 24-25 0.183818919143512 1107 26-27 0.12210227078507785 1107 28-29 0.03461339963531174 1107 30-31 0.12188962725536356 1107 32-33 0.050928561538285066 1107 34-35 0.1981839141698103 1107 36-37 0.18373564401354514 1107 38-39 0.3066847766800507 1107 40-41 0.22734454752155386 1107 42-43 0.2986512074300478 1107 44-45 0.17892510521517124 1107 46-47 0.45410438172276457 1107 48-49 0.34520695723671935 1107 50-51 0.09611082479364796 1107 52-53 0.13012901176869462 1107 54-55 0.1222357617999208 1107 56-57 -0.014965652443372335 1107 58-59 0.10652059891377519 1107 60-61 0.003730955924787338 1107 62-63 -0.16758123807611724 1107 64-65 -0.06399619348297136 1107 66-67 -0.08690320143526264 1107 68-69 0.10020613868407935 1107 70-71 -0.09962777424590286 1107 72-73 0.040908823399476546 1107 74-75 -0.1026781842304203 1107 76-77 -0.01979860079268292 1107 78-79 0.002761161268658441 1107 80-81 0.10910602043389162 1107 82-83 0.016032170459801875 1107 84-85 0.06629612230458548 1107 86-87 0.02778416399541328 1107 88-89 0.09574263159013086 1107 90-91 0.12045894624261422 1107 92-93 0.011224291799432962 1107 94-95 0.05582095253488717 1107 96-97 0.07762629263286769 1107 98-99 0.05395662537298662 1107 100-101 0.2752377343058896 1108 1 -0.04901292371280874 1108 2 -0.013310834314175679 1108 3 0.07216309497400175 1108 4 0.061361605353432935 1108 5 0.18078214773185408 1108 6 0.04419581737520417 1108 7 -0.01161818645268653 1108 8 0.0818136982986104 1108 9 0.01366480669822323 1108 10-11 -0.004775263284230391 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-1.2186577182752636 2316 94-95 -1.1214101215342396 2316 96-97 -1.0131834902250034 2316 98-99 -0.7156899231376386 2316 100-101 -1.0940603661739239 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 30.0 8 140.0 9 167.0 10 202.0 11 289.0 12 386.0 13 514.0 14 639.0 15 981.0 16 1683.0 17 2722.0 18 3945.0 19 5253.0 20 7200.0 21 9349.0 22 9927.0 23 8425.0 24 6803.0 25 6131.0 26 6554.0 27 7391.0 28 8237.0 29 9546.0 30 11234.0 31 13317.0 32 16146.0 33 20531.0 34 28381.0 35 44093.0 36 69370.0 37 147761.0 38 297077.0 39 111982.0 40 137.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.83751153987371 23.33863942689497 13.861221214833444 20.962627818397873 2 13.743070048900757 23.15772574561059 43.405780203219976 19.693424002268674 3 15.833034054604816 32.82804945527372 31.242827546433045 20.096088943688418 4 9.824970336930345 19.59868850617483 43.959499086120026 26.616842070774798 5 9.11541360599634 42.64514482881314 36.919057851936124 11.3203837132544 6 23.754185081806504 43.91136400326618 20.042652154483623 12.291798760443696 7 24.30171976146527 35.27663337567816 26.114593056852026 14.307053806004543 8 23.742642311039575 37.735820364772934 22.425042812625748 16.096494511561747 9 27.236189368852372 16.510645067335126 23.818491688530745 32.43467387528175 10-11 21.745984486039365 29.4649432572298 31.193519724700607 17.59555253203023 12-13 23.29292229804815 28.481591611473867 30.318862720235074 17.90662337024291 14-15 21.60527659604337 30.042636028467694 27.980325072663746 20.371762302825186 16-17 17.94760634117869 32.780011954125605 30.144086359501337 19.128295345194367 18-19 17.78367974286742 31.294527523557246 33.32778564187374 17.594007091701588 20-21 18.33109176199182 31.12450994814031 33.26491870801432 17.279479581853547 22-23 18.81748047969515 30.37899979452311 32.99211715918855 17.811402566593195 24-25 18.735114600067245 31.115039040609705 32.82738539993275 17.32246095939029 26-27 17.648772275563193 31.519921918780586 33.36582825867673 17.465477546979493 28-29 17.908945716740764 29.57752316284978 34.37581723764337 18.137713882766093 30-31 18.028729405611386 31.12817321702096 33.55256706018605 17.290530317181606 32-33 17.70125060709082 31.35419779952927 33.58729966002914 17.357251933350767 34-35 17.665350524900063 31.19150913438189 33.66826456083984 17.474875779878207 36-37 18.251893656367915 30.403225053237193 32.650570198378595 18.69431109201629 38-39 17.84759451937087 31.864154088990176 33.095614469309226 17.192636922329736 40-41 17.552513355998055 30.6744895767176 34.75040394889229 17.02259311839205 42-43 17.54229788545597 30.921995834422983 32.89422376433669 18.64148251578436 44-45 17.569624604103414 31.41668137821701 32.61065521542971 18.403038802249874 46-47 18.291569342147675 30.631177657390786 32.74005593490913 18.337197065552413 48-49 17.596184126193997 31.46734580246286 32.21080786428034 18.7256622070628 50-51 18.02043710688885 30.89783549378966 31.572257684651255 19.50946971467023 52-53 17.64260194365322 31.35215687557395 32.12063361505946 18.884607565713377 54-55 18.357472399906758 31.290796930966614 31.816946985971086 18.534783683155545 56-57 18.37173586290566 31.687521768705896 30.73507514148198 19.20566722690647 58-59 17.72149384071456 31.0442242712598 31.67636684251826 19.55791504550737 60-61 18.52822456895166 32.25238285919721 30.88507033855453 18.3343222332966 62-63 18.076139874543582 31.781200262147742 32.184720531785416 17.957939331523264 64-65 18.39067492351272 30.839366777690618 31.69601260739492 19.073945691401743 66-67 18.407895367332795 31.891821453156172 30.451322458834955 19.248960720676077 68-69 18.18741230625563 31.42312666293052 31.350429608037818 19.039031422776034 70-71 18.916536084498055 30.32129640341068 32.08038720096099 18.681780311130275 72-73 18.353761906931695 31.466896597652322 30.54308343527174 19.636258060144243 74-75 18.33136145865727 31.364206032065955 30.768142610964716 19.53628989831206 76-77 17.90396410572899 31.1495116893297 32.43956442512716 18.506959779814153 78-79 17.767346974563715 30.85112041829729 31.494290499168493 19.887242107970504 80-81 18.085604231310555 31.76871515364675 30.28626080189481 19.859419813147888 82-83 19.055946200969295 30.989305047164713 30.5942841451195 19.360464606746497 84-85 17.9232697956553 30.807310855085362 30.75875335184089 20.51066599741845 86-87 17.63055772509988 31.355994085857873 31.50419814128631 19.509250047755938 88-89 18.78209989868264 30.45568271189293 31.168526878315163 19.59369051110927 90-91 18.3516880408814 30.34423550753435 32.080724868739104 19.223351582845147 92-93 19.017749548762584 31.107696499496875 31.037299369641648 18.837254582098897 94-95 18.52129816021491 29.94975602799073 31.41130851762747 20.117637294166883 96-97 19.60840793537463 29.845062242700376 30.866080912589116 19.68044890933588 98-99 18.86784957317686 29.844436810779357 31.41186972605269 19.875843889991096 100-101 18.92594563431404 29.811758067615695 31.181969148346468 20.080327149723804 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 644.0 1 416.0 2 381.5 3 579.0 4 465.5 5 533.0 6 1065.5 7 1860.5 8 2645.0 9 2966.0 10 2499.0 11 1872.0 12 1759.0 13 1878.5 14 1900.5 15 1858.0 16 1806.5 17 1758.5 18 1782.5 19 1832.5 20 1901.0 21 2043.5 22 2252.5 23 2596.5 24 2877.5 25 3388.0 26 4852.5 27 6463.0 28 6876.0 29 8814.0 30 13588.0 31 18286.0 32 26547.5 33 36345.5 34 43986.0 35 51633.0 36 64137.0 37 71476.5 38 64576.5 39 58179.0 40 51147.5 41 42499.5 42 35100.0 43 31666.0 44 31137.0 45 29251.0 46 24387.0 47 17020.0 48 14781.5 49 12683.5 50 10042.5 51 7695.0 52 4908.5 53 3800.5 54 3185.0 55 2691.5 56 2141.5 57 1789.0 58 1565.5 59 1376.5 60 1223.5 61 1114.5 62 969.5 63 694.0 64 445.5 65 349.0 66 272.5 67 224.0 68 204.5 69 157.0 70 107.5 71 110.0 72 135.0 73 158.5 74 137.5 75 91.0 76 61.0 77 42.0 78 42.0 79 45.5 80 30.5 81 19.0 82 12.5 83 5.0 84 3.0 85 2.5 86 3.0 87 2.5 88 1.5 89 1.5 90 1.5 91 0.5 92 1.0 93 2.5 94 2.0 95 0.5 96 0.0 97 0.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4745815743265962 2 0.37160963126237534 3 0.19975622968580717 4 0.22719206485597937 5 0.25170919415698434 6 0.20104046026824074 7 0.19356857324317256 8 0.19333507677363918 9 0.19158385325213884 10-11 0.20611900848059175 12-13 0.19403556618223933 14-15 0.20436778495909144 16-17 0.09018801135726828 18-19 0.03876041394254119 20-21 2.3349646953338063E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.1409151193633952 46-47 0.21020519669742596 48-49 0.08761955019240109 50-51 0.06444502559121307 52-53 0.07355138790301491 54-55 0.08224913139313333 56-57 0.11184480890648932 58-59 0.09380720663503568 60-61 0.1420242275936788 62-63 0.24096835655844884 64-65 0.13694567938132776 66-67 0.10542365599432137 68-69 0.10956821832853887 70-71 0.08884540665745133 72-73 0.07413512907684837 74-75 0.13799641349422795 76-77 0.21148942727985953 78-79 0.13677055702917773 80-81 0.08563483020136736 82-83 0.08948752194866814 84-85 0.10011161131243695 86-87 0.07349301378563156 88-89 0.095383307804386 90-91 0.11628124182762357 92-93 0.16292216161691636 94-95 0.1305245264691598 96-97 0.09118037135278514 98-99 0.08008928904994957 100-101 0.06356941383046288 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 856544.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.693930290757645 #Duplication Level Percentage of deduplicated Percentage of total 1 76.97522751334128 16.698952197896354 2 9.610186285129299 4.169654227015803 3 3.4828172246149687 2.2666798225874145 4 1.81087368499487 1.5713986995058449 5 1.199005463038399 1.300557046669631 6 0.7949853524619241 1.034781409108943 7 0.6203633088345499 0.9420682863760339 8 0.47578469423522235 0.8257311992118684 9 0.37877571200488014 0.7395420502859367 >10 3.309408520582534 15.11234494405149 >50 0.6043235312911086 9.279274739780613 >100 0.6618781533188326 29.556790925998204 >500 0.05478757289177627 8.165271941131031 >1k 0.020476163606017395 6.665528115209006 >5k 0.0011068196543793187 1.671424395171826 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7387 0.8624192102215414 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6535 0.7629497142003213 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3906 0.4560186049986924 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 2560 0.29887548100272726 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 2341 0.27330761758882205 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 2038 0.23793290245451487 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 1922 0.2243901072215788 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 1921 0.2242733589868121 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1831 0.21376601785780996 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1742 0.20337542496357458 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1726 0.2015074532073075 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 1668 0.19473605559083945 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 1629 0.19018287443493853 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1613 0.1883149026786715 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 1506 0.17582284155863562 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 1480 0.17278738745470168 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1460 0.17045242275936787 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 1452 0.16951843688123436 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 1447 0.1689346957074009 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 1445 0.16870119923786753 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 1402 0.16368102514289984 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1395 0.162863787499533 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1369 0.15982833339559904 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1266 0.14780326521462997 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 1262 0.1473362722755632 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 1180 0.1377629170246946 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1173 0.13694567938132776 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 1141 0.13320973586879367 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1123 0.13110826764299324 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 1096 0.1279560653042926 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 1093 0.12760582059999254 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 1065 0.12433687002652519 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 1063 0.12410337355699183 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 1047 0.12223540180072477 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 1045 0.12200190533119139 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 1036 0.12095117121829117 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 1036 0.12095117121829117 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 1028 0.12001718534015765 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 1013 0.11826596181865731 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 971 0.11336253595845631 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 969 0.11312903948892293 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 959 0.11196155714125602 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 955 0.11149456420218927 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 943 0.11009358538498898 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 938 0.10950984421115553 No Hit GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA 919 0.10729162775058841 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 916 0.10694138304628834 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 907 0.10589064893338812 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 905 0.10565715246385475 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 895 0.10448967011618783 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 885 0.10332218776852094 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 878 0.1025049501251541 No Hit ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA 858 0.1001699854298203 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1674823476669033E-4 0.0 10-11 0.0 0.0 0.0 5.837411738334517E-4 0.0 12-13 0.0 0.0 0.0 0.001751223521500355 0.0 14-15 0.0 0.0 0.0 0.0030938282213172938 0.0 16-17 0.0 0.0 0.0 0.006829771733851384 0.0 18-19 0.0 0.0 0.0 0.011207830537602272 0.0 20-21 0.0 0.0 0.0 0.019380206971270596 0.0 22-23 0.0 0.0 0.0 0.04191261628124183 0.0 24-25 0.0 0.0 0.0 0.06934845145141405 0.0 26-27 0.0 0.0 0.0 0.08038115963686629 0.0 28-29 0.0 0.0 0.0 0.0886702843053013 0.0 30-31 0.0 0.0 0.0 0.10437292188142117 0.0 32-33 0.0 0.0 0.0 0.12883167706504278 0.0 34-35 0.0 0.0 0.0 0.16298053573429971 0.0 36-37 0.0 0.0 0.0 0.21020519669742593 0.0 38-39 0.0 0.0 0.0 0.28042925990959017 0.0 40-41 0.0 0.0 0.0 0.36548034893712406 0.0 42-43 0.0 0.0 0.0 0.44288442858743976 0.0 44-45 0.0 0.0 0.0 0.5257756752717899 0.0 46-47 0.0 0.0 0.0 0.6323084394963948 0.0 48-49 0.0 0.0 0.0 0.7412929166511002 0.0 50-51 0.0 0.0 0.0 0.8566401726005903 0.0 52-53 0.0 0.0 0.0 0.9612465909515449 0.0 54-55 0.0 0.0 0.0 1.0744923786752345 0.0 56-57 0.0 0.0 0.0 1.212897410991146 0.0 58-59 0.0 0.0 0.0 1.3706826502783278 0.0 60-61 0.0 0.0 0.0 1.535589531886278 0.0 62-63 0.0 0.0 0.0 1.6919154182388763 0.0 64-65 0.0 0.0 0.0 1.8483580528262413 0.0 66-67 0.0 0.0 0.0 2.0290259461276943 0.0 68-69 0.0 0.0 0.0 2.2433173310419545 0.0 70-71 0.0 0.0 0.0 2.4883135016998543 0.0 72-73 0.0 0.0 0.0 2.7260129076848356 0.0 74-75 0.0 0.0 0.0 2.9562988007621325 0.0 76-77 0.0 0.0 0.0 3.2066653902192996 0.0 78-79 0.0 0.0 0.0 3.509101692382411 0.0 80-81 0.0 0.0 0.0 3.8490725520230136 0.0 82-83 0.0 0.0 0.0 4.192370736354467 0.0 84-85 0.0 0.0 0.0 4.518623678409982 0.0 86-87 0.0 0.0 0.0 4.857835674524601 0.0 88-89 0.0 0.0 0.0 5.230963032838943 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCTAG 35 3.5235644E-8 81.64833 1 CCGTCCA 30 1.2980151E-4 63.36309 9 ACCGTCC 40 7.522847E-6 59.4029 8 GAACCGT 35 2.783491E-4 54.30169 6 CATTCCG 110 0.0 47.522316 9 AGCGCTC 20 5.432511E-4 47.422447 32-33 TACCTGG 215 0.0 46.474243 2 ACCTGGG 210 0.0 45.26465 3 AGAACCG 45 9.595938E-4 42.247005 5 CCGACCT 60 8.2660576E-5 39.601933 9 GAAACCG 75 6.8923946E-6 38.022305 5 GGTATCA 4075 0.0 37.8688 1 GGATGCC 270 0.0 36.961803 8 GGATGAC 790 0.0 36.0929 8 GATGACC 665 0.0 35.731064 9 GTATGGT 95 8.5149804E-7 35.094463 1 ATGGGAT 1655 0.0 34.174126 5 TGACGGC 35 2.0735593E-4 33.873177 32-33 GGGATGC 295 0.0 33.821533 7 GTACATG 6115 0.0 33.257957 1 >>END_MODULE