FastQCFastQC Report
Fri 10 Feb 2017
SRR2031632_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031632_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856544
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69730.8140854410281317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65620.7661019165390219No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38790.45286640265999173No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT24670.28801789516942505No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT22930.26770370232002094No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC20560.2400343706803153No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG19750.23057776366421343No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA19230.2245068554563455No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA18030.21049706728434267No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA17830.20816210258900883No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC17520.20454290731124147No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC16800.19613703440803976No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT16310.19041637090447192No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT16250.1897158814958718No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG15140.17675682743676915No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT15070.17593958979340232No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC14820.17302088392423506No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG14700.1716199051070348No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG14500.16928494041170097No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14040.16391452161243322No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG13720.16017857809989913No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT13710.16006182986513245No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13010.15188945343146412No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA12900.15060522284903052No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC12610.1472195240407965No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT11970.13974763701572832No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11550.13484421115552733No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA11530.13461071468599395No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG11480.1340269735121605No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC10980.12818956177382598No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG10550.1231693876788583No Hit
TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC10550.1231693876788583No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC10530.12293589120932491No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT10480.12235215003549146No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC10460.1221186535659581No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG10450.12200190533119139No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT10030.11709847947099039No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9880.11534725594949005No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA9600.11207830537602272No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG9590.11196155714125602No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC9490.11079407479358912No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT9360.10927634774162215No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9270.10822561362872193No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA9260.10810886539395524No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC9240.10787536892442186No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT9230.10775862068965517No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA9150.10682463481152164No Hit
ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT8880.10367243247282101No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT8870.10355568423805431No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA8800.10273844659468749No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT8780.1025049501251541No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA8720.10180446071655397No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA8660.10110397130795382No Hit
CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA8640.10087047483842045No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT8630.10075372660365375No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT8590.100286733664587No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCG250.00502207456.996467
CTTCGAT502.8244987E-547.505373
TTCGATA502.8254735E-547.5025984
ACCGTCC459.626639E-442.21968
GGTATCA38450.039.4335021
ATGGGCG756.9231683E-637.9976435
CATGGGT6450.037.5601964
GACATAT2450.036.860271
CATATAG2650.035.853113
GGGTATG3250.035.0747457
TGGGTAT3700.034.6600076
CATGGGG17900.033.9683384
GATGCGA352.0544996E-433.92646816-17
GTACATA2250.033.799361
ACCTGGG2400.033.649643
GGGGAGC1850.033.3763057
GTACATG62800.033.1501461
AGGGTCT1151.036442E-733.0414285
TACCTGG2450.032.962912
ATGGGTA4100.032.437015
Produced by FastQC (version 0.11.5)