##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031632_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 856544 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.5099586244256 34.0 31.0 34.0 31.0 34.0 2 32.69924370493518 34.0 31.0 34.0 31.0 34.0 3 32.77533903687376 34.0 31.0 34.0 31.0 34.0 4 36.23306683602944 37.0 37.0 37.0 35.0 37.0 5 35.990876125452985 37.0 35.0 37.0 35.0 37.0 6 36.080808458176115 37.0 37.0 37.0 35.0 37.0 7 36.034664885866924 37.0 37.0 37.0 35.0 37.0 8 36.13728891919154 37.0 37.0 37.0 35.0 37.0 9 38.00002918705869 39.0 39.0 39.0 35.0 39.0 10-11 37.973048086262935 39.0 38.5 39.0 35.0 39.0 12-13 38.003390952478796 39.0 39.0 39.0 35.0 39.0 14-15 39.379424758097656 41.0 39.0 41.0 36.0 41.0 16-17 39.35420772312923 41.0 39.0 41.0 36.0 41.0 18-19 39.47381161971831 41.0 39.5 41.0 36.5 41.0 20-21 39.53147065416371 41.0 40.0 41.0 37.0 41.0 22-23 39.4500621100609 41.0 39.0 41.0 36.0 41.0 24-25 39.38667832592371 41.0 39.0 41.0 36.0 41.0 26-27 39.24579356110135 41.0 39.0 41.0 36.0 41.0 28-29 39.155982062801215 41.0 39.0 41.0 35.0 41.0 30-31 38.98084278215713 40.0 38.5 41.0 35.0 41.0 32-33 38.75160120203982 40.0 38.0 41.0 35.0 41.0 34-35 38.62178475361453 40.0 38.0 41.0 35.0 41.0 36-37 38.45806695277767 40.0 38.0 41.0 35.0 41.0 38-39 38.305328155938284 40.0 38.0 41.0 34.0 41.0 40-41 38.17729445305787 40.0 38.0 41.0 34.0 41.0 42-43 38.00689223203945 40.0 38.0 41.0 34.0 41.0 44-45 37.880425874210786 40.0 38.0 41.0 33.0 41.0 46-47 37.72675484271677 40.0 38.0 41.0 33.0 41.0 48-49 37.695516517540256 40.0 38.0 41.0 33.0 41.0 50-51 37.586025353046665 40.0 38.0 41.0 33.0 41.0 52-53 37.52349032857623 40.0 38.0 41.0 33.0 41.0 54-55 37.449464942840066 40.0 38.0 41.0 32.0 41.0 56-57 37.37431877405014 40.0 38.0 41.0 32.0 41.0 58-59 37.21315075466059 40.0 37.0 41.0 31.5 41.0 60-61 37.00178799921545 40.0 37.0 41.0 31.0 41.0 62-63 36.80137973063847 40.0 36.5 41.0 31.0 41.0 64-65 36.52918005379759 39.0 36.0 41.0 30.0 41.0 66-67 36.264005118242615 39.0 36.0 41.0 29.0 41.0 68-69 35.94395209119438 39.0 35.0 41.0 29.0 41.0 70-71 35.600969127096796 38.0 35.0 40.0 29.0 41.0 72-73 35.20296563865955 37.0 35.0 39.5 28.0 41.0 74-75 34.77808553909665 37.0 35.0 39.0 28.0 41.0 76-77 34.09990555067807 36.0 34.0 39.0 26.0 40.0 78-79 33.98873904901558 36.0 34.0 38.0 26.0 39.5 80-81 33.656749682444804 35.5 34.0 37.0 26.0 39.0 82-83 33.29269015952479 35.0 34.0 37.0 26.0 39.0 84-85 32.912416058019204 35.0 34.0 36.0 25.5 38.0 86-87 32.64181991818283 35.0 34.0 36.0 25.0 37.0 88-89 32.34136716852841 35.0 34.0 36.0 24.5 37.0 90-91 32.13970327268652 35.0 34.0 35.5 24.0 36.5 92-93 31.98873963275675 35.0 34.0 35.0 23.0 36.0 94-95 31.829969622109314 35.0 33.0 35.0 21.5 36.0 96-97 31.69608333022005 35.0 33.0 35.0 20.0 36.0 98-99 31.59090192681287 35.0 33.0 35.0 20.0 36.0 100-101 31.183092170396385 34.5 32.0 35.0 19.0 35.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22571836925287414 1101 2 0.2271445296603929 1101 3 0.25404775218180475 1101 4 0.19709319526398872 1101 5 0.2673679916387641 1101 6 0.19366387747978564 1101 7 0.28330940135575844 1101 8 0.187678726431038 1101 9 0.310314855253516 1101 10-11 0.13022657408610883 1101 12-13 0.18954334244247661 1101 14-15 0.3370630655802742 1101 16-17 0.300617371674214 1101 18-19 0.31287261065316585 1101 20-21 0.17183411536692716 1101 22-23 0.21090680844284293 1101 24-25 0.26217005373305113 1101 26-27 0.24193711359450987 1101 28-29 0.20807629268662708 1101 30-31 0.17002614357994617 1101 32-33 0.1999099842003318 1101 34-35 0.2740468252128494 1101 36-37 0.21958805279074056 1101 38-39 0.3557146330082759 1101 40-41 0.30797973205671525 1101 42-43 0.0797017123837307 1101 44-45 0.14826363831981837 1101 46-47 0.3431926178994331 1101 48-49 0.46655638512709885 1101 50-51 0.5460280084619811 1101 52-53 0.2255351897056883 1101 54-55 0.21702945690380915 1101 56-57 0.5788746429005514 1101 58-59 0.4066935480628757 1101 60-61 0.4994827737671059 1101 62-63 0.4102555149240743 1101 64-65 0.28141463438409176 1101 66-67 0.2646316343957906 1101 68-69 0.26563216375081566 1101 70-71 0.4054661178887642 1101 72-73 0.5717356063378816 1101 74-75 0.5263882098000252 1101 76-77 0.3807974900579225 1101 78-79 0.37357774913089514 1101 80-81 0.4865712930278505 1101 82-83 0.4111031215894059 1101 84-85 0.34230513078079383 1101 86-87 0.273022751563353 1101 88-89 0.23218248806271902 1101 90-91 0.24671068560777343 1101 92-93 0.2104885461071646 1101 94-95 0.2797014714098687 1101 96-97 0.27016236351159506 1101 98-99 0.08180731711891553 1101 100-101 0.08919572035390644 1102 1 0.12536560646816497 1102 2 0.1517208607702969 1102 3 0.36539980001298034 1102 4 0.19355771078476636 1102 5 -0.019799771028090163 1102 6 0.09605307932451979 1102 7 0.1309064504047086 1102 8 -0.013513910333287527 1102 9 0.1283552540561601 1102 10-11 0.2750125201424751 1102 12-13 0.11475998907472729 1102 14-15 0.18569719007646768 1102 16-17 0.17787320718776556 1102 18-19 0.1858190834181528 1102 20-21 0.1581242922197177 1102 22-23 0.13490960540390518 1102 24-25 0.3138588368192927 1102 26-27 0.26039234257606125 1102 28-29 -0.22065619684440918 1102 30-31 -0.09515483394223168 1102 32-33 0.14882616179164643 1102 34-35 -0.014130514688631024 1102 36-37 0.1505518258597789 1102 38-39 0.22569915544288932 1102 40-41 0.061607531964014584 1102 42-43 0.27625107953200967 1102 44-45 0.20901897493722998 1102 46-47 0.19438014785283997 1102 48-49 0.14953734850733724 1102 50-51 0.2273905056139469 1102 52-53 0.20160781641352798 1102 54-55 0.13323454643013122 1102 56-57 -0.08787059008788134 1102 58-59 0.07154533269687136 1102 60-61 0.24201308072572658 1102 62-63 0.17238419084585388 1102 64-65 0.22459547048688222 1102 66-67 0.4150704273028154 1102 68-69 0.2718506881181568 1102 70-71 0.4208140488446901 1102 72-73 0.5235783794147721 1102 74-75 0.4071363183158354 1102 76-77 0.5341314519218301 1102 78-79 0.2354856536885208 1102 80-81 0.1539332716420958 1102 82-83 0.13814800652902193 1102 84-85 0.306254974040975 1102 86-87 0.3277874061465198 1102 88-89 0.31171910389698354 1102 90-91 0.36499621133432214 1102 92-93 0.4218201666946797 1102 94-95 0.3141056637087978 1102 96-97 0.29150097650175155 1102 98-99 0.3378471031042558 1102 100-101 0.3975253217869543 1103 1 -0.10655034725229484 1103 2 0.04991289430068235 1103 3 0.12714181739409725 1103 4 -0.036472910223295685 1103 5 -0.01969962055199659 1103 6 -0.07335645850606909 1103 7 0.11118431790062289 1103 8 -0.042404818923444054 1103 9 0.20282464431502945 1103 10-11 0.08590964306966953 1103 12-13 0.02105419612081505 1103 14-15 -0.018717442909597537 1103 16-17 0.10491734099881711 1103 18-19 0.008969617095004878 1103 20-21 -0.14472699183457394 1103 22-23 -0.17712798106977345 1103 24-25 -0.10217250026467894 1103 26-27 0.018755533270073954 1103 28-29 0.1234933879066844 1103 30-31 0.06113749528243062 1103 32-33 0.04285193551666566 1103 34-35 -0.3079557370852797 1103 36-37 -0.07580702831862851 1103 38-39 0.16457982364686785 1103 40-41 0.066979353125312 1103 42-43 -0.03301233875395582 1103 44-45 0.1828546630586274 1103 46-47 0.1680180387839627 1103 48-49 -0.2330412258005552 1103 50-51 -0.052349804201547556 1103 52-53 -0.003059696524665867 1103 54-55 -0.04959390331561053 1103 56-57 0.1477409162003127 1103 58-59 0.01589797253824088 1103 60-61 0.13962424149477926 1103 62-63 -0.13178782761188046 1103 64-65 0.03830306614937484 1103 66-67 0.09640413119291935 1103 68-69 0.11537683615558336 1103 70-71 0.07906181611501495 1103 72-73 -0.05057804060630389 1103 74-75 0.021976365486708005 1103 76-77 -0.09505014342518336 1103 78-79 0.2133348721392494 1103 80-81 0.21448049384346035 1103 82-83 0.25176246224873466 1103 84-85 0.14137223772967644 1103 86-87 0.185498617241187 1103 88-89 0.2304682745258475 1103 90-91 0.3354618359352841 1103 92-93 0.014617094078069215 1103 94-95 0.1857715917657039 1103 96-97 0.044941771295928135 1103 98-99 -0.10413071857281508 1103 100-101 -0.06717664291500469 1104 1 0.18739941269870997 1104 2 0.1864951724924424 1104 3 0.21456718055246427 1104 4 0.13216464893204005 1104 5 0.10885055665693955 1104 6 0.10845009011270434 1104 7 0.1914916171723675 1104 8 0.16782371320153544 1104 9 0.2835546857437592 1104 10-11 0.15455954172580277 1104 12-13 0.1815088381724692 1104 14-15 0.32285479229956593 1104 16-17 0.37024974201430894 1104 18-19 0.2892853712098784 1104 20-21 0.28622093318696784 1104 22-23 0.25920705008746836 1104 24-25 0.386534571411822 1104 26-27 0.4211843583439432 1104 28-29 0.3155005905190009 1104 30-31 0.33912359734838304 1104 32-33 0.4333480062997026 1104 34-35 0.3788825857480518 1104 36-37 0.5258887390040883 1104 38-39 0.5981910242574386 1104 40-41 0.5128900654115611 1104 42-43 0.440727375472342 1104 44-45 0.49286176969705053 1104 46-47 0.7507761000198769 1104 48-49 0.6230230675687878 1104 50-51 0.7104072972954185 1104 52-53 0.6830645348272668 1104 54-55 0.5717056746277578 1104 56-57 0.3917613997716458 1104 58-59 0.5334314891360066 1104 60-61 0.829596569021696 1104 62-63 0.7283587839383472 1104 64-65 0.5535480623675397 1104 66-67 0.5505251816957539 1104 68-69 0.5483527809843096 1104 70-71 0.5311822679235476 1104 72-73 0.4807460106803205 1104 74-75 0.5190994337264314 1104 76-77 0.4690192868326477 1104 78-79 0.6471882925803172 1104 80-81 0.5686515878773051 1104 82-83 0.5504252259770084 1104 84-85 0.5352479606758891 1104 86-87 0.4393918439977895 1104 88-89 0.49652162230096764 1104 90-91 0.571866105402556 1104 92-93 0.5967398995321318 1104 94-95 0.49242760990019363 1104 96-97 0.4190857184280219 1104 98-99 0.5529282756664635 1104 100-101 0.4949184672150402 1105 1 0.24363588108518996 1105 2 0.22873827060504937 1105 3 0.15895454248413188 1105 4 0.06273600411167024 1105 5 0.07901678908878296 1105 6 0.0923405895439231 1105 7 0.23651137179910364 1105 8 0.25089711723563823 1105 9 0.2465652174673636 1105 10-11 0.26767465676745417 1105 12-13 0.22091106194407217 1105 14-15 0.21854669351419886 1105 16-17 0.1660187046211874 1105 18-19 0.10005989936176007 1105 20-21 0.15249189572044486 1105 22-23 0.15517417056010885 1105 24-25 0.228629051125111 1105 26-27 0.1193880939866716 1105 28-29 0.20223257588956045 1105 30-31 0.22327157968541655 1105 32-33 0.33132116936405254 1105 34-35 0.29889469539540414 1105 36-37 0.2966188651156685 1105 38-39 0.13589912059641307 1105 40-41 0.03040318419619581 1105 42-43 0.1880760966201862 1105 44-45 0.22022345672994703 1105 46-47 0.33276085898896923 1105 48-49 0.2670972911446796 1105 50-51 0.34714845664124994 1105 52-53 0.23543570163460004 1105 54-55 0.1275384881024948 1105 56-57 0.12982605794932311 1105 58-59 0.0962762777064583 1105 60-61 0.13623057633640911 1105 62-63 0.1736574274132039 1105 64-65 0.43409984639993127 1105 66-67 0.40944473204864806 1105 68-69 0.21497051978288795 1105 70-71 0.24027245421640941 1105 72-73 0.2852485977413437 1105 74-75 0.2225873791229347 1105 76-77 -0.05397986916162267 1105 78-79 -0.18126142791923172 1105 80-81 -0.21119746964963326 1105 82-83 0.11138323363421421 1105 84-85 -0.033890174927698524 1105 86-87 0.025196034669697553 1105 88-89 0.04472474634534507 1105 90-91 -0.04197375368517697 1105 92-93 -0.0838222816722265 1105 94-95 -0.08864586752570247 1105 96-97 0.11453114550715071 1105 98-99 -0.18364714052620812 1105 100-101 0.16477767727460702 1106 1 0.07884168194409824 1106 2 0.120409959578339 1106 3 0.17212814751477623 1106 4 0.13032298477673265 1106 5 0.115131911727822 1106 6 -0.0046714222973491815 1106 7 0.2317108047522609 1106 8 0.2114938051665618 1106 9 0.1560878898651481 1106 10-11 0.18145965655898522 1106 12-13 0.12432073433806323 1106 14-15 0.038774649378552795 1106 16-17 0.08532066085699341 1106 18-19 0.06320090677960621 1106 20-21 0.07278978483549992 1106 22-23 0.06765526583989612 1106 24-25 0.143853462946673 1106 26-27 0.14640905121935788 1106 28-29 0.06351267656312132 1106 30-31 0.1704094334737576 1106 32-33 0.07783493048420098 1106 34-35 0.16494030172995622 1106 36-37 0.041709323491176065 1106 38-39 0.11277661042861098 1106 40-41 0.16448266172469772 1106 42-43 0.08787634148132639 1106 44-45 -0.019217380498048442 1106 46-47 0.1528675214103714 1106 48-49 0.23537147497247446 1106 50-51 0.44460394763331834 1106 52-53 0.37578927202080337 1106 54-55 0.29565499045445165 1106 56-57 0.23100184626194675 1106 58-59 0.1470385311870075 1106 60-61 0.3684231827429798 1106 62-63 0.116924314682322 1106 64-65 0.05871654016809913 1106 66-67 0.0754319659137721 1106 68-69 0.1256435846786701 1106 70-71 0.22271238553399542 1106 72-73 0.15245312501910036 1106 74-75 -0.01422385416605465 1106 76-77 0.11514204211979973 1106 78-79 0.17684893395216505 1106 80-81 0.11735424873731404 1106 82-83 0.12877178580745863 1106 84-85 0.17440193758712752 1106 86-87 0.21392389387833788 1106 88-89 0.17602774115625408 1106 90-91 0.22479316959292817 1106 92-93 0.10754465752564357 1106 94-95 0.19071377890390906 1106 96-97 0.04352804873393623 1106 98-99 0.13020627634798032 1106 100-101 0.22029975078601538 1107 1 0.03799710383515986 1107 2 0.0031262866810450873 1107 3 0.04642257317467369 1107 4 0.10889359758383677 1107 5 0.023664213275843338 1107 6 0.1297294581348325 1107 7 0.09481121347490529 1107 8 0.08142933012079112 1107 9 0.11399914756113816 1107 10-11 0.04693418666637683 1107 12-13 -0.044068843061410234 1107 14-15 0.05105795888991338 1107 16-17 -0.0018962874626069492 1107 18-19 0.0043955466641563135 1107 20-21 -0.07966618490310395 1107 22-23 0.016427367491047562 1107 24-25 0.05435064411013002 1107 26-27 0.022475415871944904 1107 28-29 -0.21686984768856377 1107 30-31 -0.11478103266386341 1107 32-33 -0.03127169386819162 1107 34-35 0.10053274191836437 1107 36-37 0.11448514206122695 1107 38-39 0.040965494972247996 1107 40-41 0.020416580859212274 1107 42-43 -0.2835473353612983 1107 44-45 -0.46723848456831973 1107 46-47 -0.24588364228980453 1107 48-49 -0.6019198809415442 1107 50-51 -0.6952641605795478 1107 52-53 -0.762311223222305 1107 54-55 -0.6313520246243343 1107 56-57 -0.6290340078161094 1107 58-59 -0.4657746296129943 1107 60-61 -0.3173682545002734 1107 62-63 -0.6220434616380643 1107 64-65 -0.6013099697004236 1107 66-67 -0.3149189264582404 1107 68-69 -0.11330861795917002 1107 70-71 -0.15340675659835767 1107 72-73 -0.07576102349845826 1107 74-75 -0.32895932651584303 1107 76-77 -0.4645571435496407 1107 78-79 -0.30825570389049517 1107 80-81 -0.25647210124140685 1107 82-83 -0.13995643266708413 1107 84-85 -0.05240192745479533 1107 86-87 -0.255190032800769 1107 88-89 -0.394137755177006 1107 90-91 -0.17158646833625824 1107 92-93 -0.12200277125936765 1107 94-95 -0.22215579323512102 1107 96-97 -0.18439712145180565 1107 98-99 -0.12777696636959135 1107 100-101 -0.18813305223458698 1108 1 0.1418899066217847 1108 2 0.11686014512172704 1108 3 0.11351968142314206 1108 4 0.086619422509699 1108 5 0.16203993699824792 1108 6 0.2666287096999582 1108 7 0.19546550559986287 1108 8 0.18969349468232366 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-0.8095540114585269 2316 100-101 -1.1234364392371567 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 18.0 14 78.0 15 289.0 16 727.0 17 1667.0 18 2905.0 19 4211.0 20 5934.0 21 8366.0 22 9403.0 23 7698.0 24 5777.0 25 5296.0 26 5763.0 27 6604.0 28 7883.0 29 9200.0 30 10632.0 31 12866.0 32 15784.0 33 19718.0 34 26773.0 35 41619.0 36 65828.0 37 146463.0 38 316004.0 39 118904.0 40 133.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.110445362476774 23.676357397226514 14.155684734150217 21.057512506146495 2 13.873468086820381 23.376963014592654 42.947040782135296 19.802528116451672 3 15.95745674457702 32.53134704742335 31.478506549606557 20.032689658393068 4 9.782329478580904 19.850612774861283 43.59120163655725 26.77585611000056 5 8.970817611237718 42.66319068255688 37.15150651922143 11.214485186983973 6 23.670938095416 44.03077950461389 19.825017745731685 12.473264654238427 7 24.10500803227855 35.32031045690589 26.048982889378713 14.525698621436845 8 23.758616019725782 37.86472148541114 22.389742967086338 15.986919527776742 9 26.952614226472893 16.391685657712856 23.773326297306387 32.882373818507865 10-11 21.681431426756827 29.486751410318675 31.11743237942242 17.714384783502073 12-13 23.232081480928006 28.412667650465124 30.362888538125304 17.992362330481562 14-15 21.440054451376696 30.047318059550935 27.98297577240632 20.529651716666045 16-17 17.845259554675533 32.82586767288079 30.096702562857246 19.232170209586428 18-19 17.70685452235962 31.296640919789294 33.26320656031681 17.73329799753428 20-21 18.291121063249523 31.051119382074944 33.259704113273806 17.398055441401723 22-23 18.752046774749996 30.491792177861733 32.86713082604764 17.88903022134063 24-25 18.5971765606904 31.15385782866963 32.83520753166212 17.413758078977846 26-27 17.610070235738036 31.47876816602533 33.357364011656145 17.55379758658049 28-29 17.78847164386857 29.58499833013763 34.43980204540624 18.18672798058756 30-31 18.012487272183765 30.990072303337723 33.55215835738774 17.445282067090773 32-33 17.566835523727356 31.32171998370193 33.64191873612884 17.469525756441882 34-35 17.619877087458438 31.172712668584452 33.658632831471586 17.54877741248552 36-37 18.14728723801696 30.464109257668028 32.68536117233907 18.703242331975943 38-39 17.83475221354653 31.85714919490417 33.02539040609706 17.282708185452236 40-41 17.436056991818283 30.633102327492807 34.824830948556055 17.10600973213285 42-43 17.559343127731907 30.87132710053424 32.844372268091306 18.724957503642546 44-45 17.558175645384242 31.31964032203833 32.641171965479884 18.481012067097545 46-47 18.26210912691 30.57893114656106 32.725872809803114 18.43308691672582 48-49 17.454466039089922 31.320287787596357 32.25149239742998 18.973753775883743 50-51 17.97721650971127 30.724668648093562 31.609013215545268 19.6891016266499 52-53 17.537452833713154 31.26675337168902 32.2380403108305 18.957753483767327 54-55 18.3443496372296 31.18576214473806 31.82490819098144 18.6449800270509 56-57 18.36659879702619 31.58851150670602 30.6545256285725 19.390364067695295 58-59 17.55064538424179 30.96017250719169 31.70596023088131 19.783221877685207 60-61 18.447388575484737 32.23658095789592 30.928008368513467 18.38802209810588 62-63 18.043439683191988 31.666907946351852 32.13670284305301 18.152949527403145 64-65 18.17676616729555 30.891991538087943 31.645484645272166 19.285757649344344 66-67 18.243312661112565 31.794338644599694 30.53188160795009 19.430467086337654 68-69 17.999133728098034 31.408018735756716 31.263309298763403 19.32953823738185 70-71 18.64708643105316 30.2675636044383 32.139738297156946 18.94561166735159 72-73 18.12387921694624 31.416424608659916 30.457688104755853 20.002008069637984 74-75 17.991720215190345 31.246906171778683 30.812018997272762 19.94935461575821 76-77 17.487893208054693 31.22133830836478 32.22513963088878 19.065628852691745 78-79 17.327521592636437 30.780510471173628 31.42537085163366 20.466597084556273 80-81 17.703004307881283 31.616952934397492 30.20695227470026 20.473090483020968 82-83 18.494957974363135 30.888580398621833 30.629853321829163 19.986608305185865 84-85 17.414633690738597 30.71797829416819 30.714183976538273 21.153204038554936 86-87 16.976827810363506 31.315962752643177 31.416949975716367 20.290259461276946 88-89 18.139815350991892 30.364289516942506 31.000392274068815 20.495502857996787 90-91 17.583159767624313 30.26015009153062 31.9877904696081 20.16889967123697 92-93 18.085469047708 31.14119064519744 30.90168164157358 19.87165866552098 94-95 17.490286546867413 29.842775152239696 31.29751653155004 21.369421769342846 96-97 18.39561073336571 29.820534613516642 30.809450536107896 20.97440411700975 98-99 17.53546811372212 29.743947771509692 31.441350356782603 21.27923375798558 100-101 17.438450330631 29.736826129188927 31.211881701348677 21.612841838831397 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 612.0 1 410.5 2 411.0 3 631.0 4 505.5 5 524.5 6 1011.0 7 1828.0 8 2605.5 9 2845.0 10 2382.0 11 1863.5 12 1743.0 13 1740.0 14 1762.5 15 1732.5 16 1670.0 17 1637.0 18 1609.0 19 1646.5 20 1768.5 21 1906.5 22 2077.5 23 2342.5 24 2656.0 25 3195.5 26 4577.5 27 6159.5 28 6593.0 29 8436.5 30 12980.0 31 17667.5 32 25988.0 33 36000.5 34 43943.5 35 51991.0 36 64477.5 37 71689.5 38 65201.5 39 58553.0 40 51353.0 41 42982.0 42 35698.5 43 32201.5 44 31516.0 45 29563.5 46 24713.5 47 17375.0 48 15113.5 49 12965.0 50 10322.5 51 8122.0 52 5129.0 53 3788.0 54 3142.5 55 2625.0 56 2105.0 57 1809.5 58 1623.0 59 1375.0 60 1164.0 61 1042.0 62 895.0 63 624.0 64 380.0 65 262.5 66 214.0 67 183.5 68 178.5 69 151.0 70 88.0 71 86.5 72 124.5 73 141.0 74 110.0 75 88.5 76 64.0 77 28.5 78 35.0 79 40.5 80 26.5 81 14.0 82 6.0 83 2.0 84 1.0 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04261310568984197 2 0.001984719991033736 3 4.6699293906676126E-4 4 0.013075802293869319 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0015761011693503195 24-25 0.0 26-27 0.0 28-29 0.021715171666604402 30-31 0.01774573168453693 32-33 1.1674823476669031E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.03356511749542347 50-51 0.010565715246385474 52-53 0.0 54-55 0.0012258564650502483 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.3349646953338063E-4 80-81 0.010565715246385474 82-83 0.005020174094967684 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 856544.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.50201270461897 #Duplication Level Percentage of deduplicated Percentage of total 1 75.47347231581296 15.473580882805052 2 10.20460188455974 4.184297549656449 3 3.6317213061227247 2.233727890732905 4 1.9347023787843156 1.586611709979703 5 1.2540272299005801 1.2855041099679914 6 0.8981157663077438 1.104790851063598 7 0.6713954249987463 0.9635470273203144 8 0.5288382988850568 0.8673799617944415 9 0.4319439751918776 0.7970148780360744 >10 3.5337982717468033 15.409546749687191 >50 0.6354249247797354 9.262714807809745 >100 0.7198726610655181 30.201377957829127 >500 0.06023544825978905 8.532521578531913 >1k 0.020669026363653104 6.458656576989881 >5k 0.0011810872207801773 1.638727467795633 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6973 0.8140854410281317 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6562 0.7661019165390219 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3879 0.45286640265999173 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 2467 0.28801789516942505 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 2293 0.26770370232002094 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 2056 0.2400343706803153 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1975 0.23057776366421343 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 1923 0.2245068554563455 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1803 0.21049706728434267 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 1783 0.20816210258900883 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 1752 0.20454290731124147 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1680 0.19613703440803976 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1631 0.19041637090447192 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1625 0.1897158814958718 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 1514 0.17675682743676915 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1507 0.17593958979340232 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 1482 0.17302088392423506 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 1470 0.1716199051070348 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 1450 0.16928494041170097 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1404 0.16391452161243322 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 1372 0.16017857809989913 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 1371 0.16006182986513245 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1301 0.15188945343146412 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1290 0.15060522284903052 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1261 0.1472195240407965 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 1197 0.13974763701572832 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1155 0.13484421115552733 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 1153 0.13461071468599395 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 1148 0.1340269735121605 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 1098 0.12818956177382598 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 1055 0.1231693876788583 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 1055 0.1231693876788583 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 1053 0.12293589120932491 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 1048 0.12235215003549146 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 1046 0.1221186535659581 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 1045 0.12200190533119139 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 1003 0.11709847947099039 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 988 0.11534725594949005 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 960 0.11207830537602272 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 959 0.11196155714125602 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 949 0.11079407479358912 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 936 0.10927634774162215 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 927 0.10822561362872193 No Hit GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA 926 0.10810886539395524 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 924 0.10787536892442186 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 923 0.10775862068965517 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 915 0.10682463481152164 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 888 0.10367243247282101 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 887 0.10355568423805431 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 880 0.10273844659468749 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 878 0.1025049501251541 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 872 0.10180446071655397 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 866 0.10110397130795382 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 864 0.10087047483842045 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 863 0.10075372660365375 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 859 0.100286733664587 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1674823476669033E-4 0.0 10-11 0.0 0.0 0.0 5.837411738334517E-4 0.0 12-13 0.0 0.0 0.0 0.0018679717562670455 0.0 14-15 0.0 0.0 0.0 0.003210576456083984 0.0 16-17 0.0 0.0 0.0 0.00688814585123473 1.1674823476669033E-4 18-19 0.0 0.0 0.0 0.011207830537602272 1.1674823476669033E-4 20-21 0.0 0.0 0.0 0.019380206971270596 1.1674823476669033E-4 22-23 0.0 0.0 0.0 0.04156237157694176 1.1674823476669033E-4 24-25 0.0 0.0 0.0 0.06905658086449733 1.1674823476669033E-4 26-27 0.0 0.0 0.0 0.08043953375424964 1.1674823476669033E-4 28-29 0.0 0.0 0.0 0.08861191018791796 1.1674823476669033E-4 30-31 0.0 0.0 0.0 0.10402267717712108 1.1674823476669033E-4 32-33 0.0 0.0 0.0 0.12848143236074272 1.1674823476669033E-4 34-35 0.0 0.0 0.0 0.16239679456046624 1.1674823476669033E-4 36-37 0.0 0.0 0.0 0.2102635708148093 1.1674823476669033E-4 38-39 0.0 0.0 0.0 0.2801373893226734 1.1674823476669033E-4 40-41 0.0 0.0 0.0 0.3661224642283409 1.1674823476669033E-4 42-43 0.0 0.0 0.0 0.44352654387865653 1.1674823476669033E-4 44-45 0.0 0.0 0.0 0.5279355176149736 1.1674823476669033E-4 46-47 0.0 0.0 0.0 0.6352855194829454 1.1674823476669033E-4 48-49 0.0 0.0 0.0 0.7435111331116673 1.1674823476669033E-4 50-51 0.0 0.0 0.0 0.8591502596480741 1.1674823476669033E-4 52-53 0.0 0.0 0.0 0.9641069227033288 1.1674823476669033E-4 54-55 0.0 0.0 0.0 1.0786953151268353 1.1674823476669033E-4 56-57 0.0 0.0 0.0 1.2173922180296635 1.1674823476669033E-4 58-59 0.0 0.0 0.0 1.3754109537863788 1.1674823476669033E-4 60-61 0.0 0.0 0.0 1.5424776777375127 1.1674823476669033E-4 62-63 0.0 0.0 0.0 1.700846658198528 1.1674823476669033E-4 64-65 0.0 0.0 0.0 1.8586318974857101 1.1674823476669033E-4 66-67 0.0 0.0 0.0 2.04064239548698 1.1674823476669033E-4 68-69 0.0 0.0 0.0 2.2546419098143238 1.1674823476669033E-4 70-71 0.0 0.0 0.0 2.4999299510591397 1.1674823476669033E-4 72-73 0.0 0.0 0.0 2.737979601748422 1.1674823476669033E-4 74-75 0.0 0.0 0.0 2.9686157395300183 1.1674823476669033E-4 76-77 0.0 0.0 0.0 3.221025423095603 1.1674823476669033E-4 78-79 0.0 0.0 0.0 3.5250378264280644 1.1674823476669033E-4 80-81 0.0 0.0 0.0 3.868277636642134 1.1674823476669033E-4 82-83 0.0 0.0 0.0 4.214319404490604 1.1674823476669033E-4 84-85 0.0 0.0 0.0 4.542090073598088 1.1674823476669033E-4 86-87 0.0 0.0 0.0 4.883578660290656 1.1674823476669033E-4 88-89 0.0 0.0 0.0 5.256997889191915 1.1674823476669033E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGACCG 25 0.005022074 56.99646 7 CTTCGAT 50 2.8244987E-5 47.50537 3 TTCGATA 50 2.8254735E-5 47.502598 4 ACCGTCC 45 9.626639E-4 42.2196 8 GGTATCA 3845 0.0 39.433502 1 ATGGGCG 75 6.9231683E-6 37.997643 5 CATGGGT 645 0.0 37.560196 4 GACATAT 245 0.0 36.86027 1 CATATAG 265 0.0 35.85311 3 GGGTATG 325 0.0 35.074745 7 TGGGTAT 370 0.0 34.660007 6 CATGGGG 1790 0.0 33.968338 4 GATGCGA 35 2.0544996E-4 33.926468 16-17 GTACATA 225 0.0 33.79936 1 ACCTGGG 240 0.0 33.64964 3 GGGGAGC 185 0.0 33.376305 7 GTACATG 6280 0.0 33.150146 1 AGGGTCT 115 1.036442E-7 33.041428 5 TACCTGG 245 0.0 32.96291 2 ATGGGTA 410 0.0 32.43701 5 >>END_MODULE