FastQCFastQC Report
Fri 10 Feb 2017
SRR2031631_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031631_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13327
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA459.117181E-442.3781971
AACGCAG459.289088E-442.218886
TCAACGC459.289088E-442.218884
ATCAACG500.001559659537.9969943
CAACGCA500.001559659537.9969945
TATCAAC550.002444440534.673072
Produced by FastQC (version 0.11.5)