##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031629_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1184000 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30307347972973 34.0 31.0 34.0 31.0 34.0 2 32.51788006756757 34.0 31.0 34.0 31.0 34.0 3 32.64300168918919 34.0 31.0 34.0 31.0 34.0 4 35.76608192567568 37.0 35.0 37.0 35.0 37.0 5 35.59431672297297 37.0 35.0 37.0 33.0 37.0 6 35.79864864864865 37.0 37.0 37.0 35.0 37.0 7 35.89550844594594 37.0 37.0 37.0 35.0 37.0 8 35.95106756756757 37.0 37.0 37.0 35.0 37.0 9 37.855793074324325 39.0 39.0 39.0 35.0 39.0 10-11 37.82029814189189 39.0 39.0 39.0 35.0 39.0 12-13 37.89957010135135 39.0 39.0 39.0 35.0 39.0 14-15 39.3515316722973 41.0 39.5 41.0 37.0 41.0 16-17 39.32316258445946 41.0 39.0 41.0 36.5 41.0 18-19 39.32220565878379 41.0 39.5 41.0 36.5 41.0 20-21 39.37030278716216 41.0 39.0 41.0 36.5 41.0 22-23 39.294045185810816 41.0 39.0 41.0 36.0 41.0 24-25 39.22082939189189 41.0 39.0 41.0 36.0 41.0 26-27 39.142995777027025 41.0 39.0 41.0 36.0 41.0 28-29 39.0760004222973 41.0 39.0 41.0 36.0 41.0 30-31 38.91597930743244 41.0 39.0 41.0 35.0 41.0 32-33 38.76674662162162 40.0 38.0 41.0 35.0 41.0 34-35 38.7213754222973 40.0 38.0 41.0 35.0 41.0 36-37 38.60254518581081 40.0 38.0 41.0 35.0 41.0 38-39 38.43379138513514 40.0 38.0 41.0 34.5 41.0 40-41 38.39590160472973 40.0 38.0 41.0 34.0 41.0 42-43 38.291041807432435 40.0 38.0 41.0 34.0 41.0 44-45 38.07885810810811 40.0 38.0 41.0 33.5 41.0 46-47 37.88951055743243 40.0 38.0 41.0 33.0 41.0 48-49 37.98458361486486 40.0 38.0 41.0 33.0 41.0 50-51 37.77432136824324 39.5 38.0 40.5 33.0 41.0 52-53 37.959195523648646 40.0 38.0 41.0 33.5 41.0 54-55 38.28446790540541 41.0 39.0 41.0 34.0 41.0 56-57 38.225414273648646 41.0 39.0 41.0 34.0 41.0 58-59 38.12205954391892 41.0 38.5 41.0 34.0 41.0 60-61 37.91795988175676 40.0 38.0 41.0 33.0 41.0 62-63 37.64351140202703 40.0 38.0 41.0 33.0 41.0 64-65 37.45924493243243 40.0 37.0 41.0 32.0 41.0 66-67 37.27752195945946 39.5 37.0 41.0 32.0 41.0 68-69 37.02305701013513 39.0 36.5 41.0 32.0 41.0 70-71 36.67797888513513 39.0 36.0 41.0 31.5 41.0 72-73 36.3215004222973 38.5 36.0 40.0 31.0 41.0 74-75 35.84487880067567 37.0 35.0 39.5 31.0 41.0 76-77 35.402019425675675 37.0 35.0 39.0 30.5 41.0 78-79 34.984125 36.0 35.0 39.0 30.0 40.5 80-81 34.55112964527027 36.0 35.0 37.5 30.0 39.0 82-83 34.15375675675676 35.5 35.0 37.0 29.5 39.0 84-85 33.76567863175676 35.0 34.0 36.5 29.0 38.0 86-87 33.50696537162162 35.0 34.0 36.0 29.0 37.0 88-89 33.22165371621622 35.0 34.0 36.0 29.0 37.0 90-91 33.01697466216216 35.0 34.0 36.0 29.0 36.5 92-93 32.83391554054054 35.0 34.0 35.0 29.0 36.0 94-95 32.65782474662162 35.0 34.0 35.0 27.5 36.0 96-97 32.54816596283784 35.0 34.0 35.0 27.0 36.0 98-99 32.46630025337838 35.0 34.0 35.0 27.0 36.0 100-101 32.073390625 35.0 33.0 35.0 26.0 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21950246748566826 1101 2 0.2220107053122149 1101 3 0.20448569168223685 1101 4 0.18341075501534476 1101 5 0.17716613749355048 1101 6 0.19934278420412 1101 7 0.17978648835438094 1101 8 0.24866655097766 1101 9 0.287855502889542 1101 10-11 0.3895550895975788 1101 12-13 0.30822645134198723 1101 14-15 0.2958902403793573 1101 16-17 0.2815134826327679 1101 18-19 0.2690533185863089 1101 20-21 0.1888090524295123 1101 22-23 0.2073837532008227 1101 24-25 0.2869335236773978 1101 26-27 0.3530764364926995 1101 28-29 0.4365630847201487 1101 30-31 0.3323683808611477 1101 32-33 0.28773078902631255 1101 34-35 0.2196148242274205 1101 36-37 0.2831833417719949 1101 38-39 0.2175592540119311 1101 40-41 0.21832535571915201 1101 42-43 -0.01943383468916693 1101 44-45 0.3013869396214446 1101 46-47 0.25189117879862977 1101 48-49 0.3324689220843382 1101 50-51 0.24525600960546257 1101 52-53 0.22409093227862087 1101 54-55 0.261503015130252 1101 56-57 0.18878722568189232 1101 58-59 0.20569226643787886 1101 60-61 0.09802438930214663 1101 62-63 -0.02113879971332011 1101 64-65 0.17048390138127445 1101 66-67 0.038360609925931044 1101 68-69 0.05106472288647268 1101 70-71 -0.1001674550497782 1101 72-73 0.052478455850483385 1101 74-75 0.008394136945845787 1101 76-77 -0.07283922069910176 1101 78-79 0.004738187135096439 1101 80-81 0.07711172626503782 1101 82-83 0.15855200379272105 1101 84-85 0.17490258587074692 1101 86-87 0.32131449103889764 1101 88-89 0.33697552224509053 1101 90-91 0.08691698058019881 1101 92-93 0.11714042491858123 1101 94-95 0.1580911190159 1101 96-97 0.04998493301232543 1101 98-99 0.13897896836072476 1101 100-101 0.3331405902474174 1102 1 -0.2374826267837804 1102 2 -0.19846822353509452 1102 3 -0.12314283805770287 1102 4 -0.20210121820583282 1102 5 -0.13605447304059481 1102 6 -0.09913231092362196 1102 7 -0.10008648392267361 1102 8 0.04793413888141629 1102 9 0.013744553900899348 1102 10-11 0.02173544607628486 1102 12-13 0.08452033169560735 1102 14-15 0.007663192629514981 1102 16-17 0.06031072464346465 1102 18-19 0.19429292605921233 1102 20-21 0.1239133353236852 1102 22-23 0.07084039497691919 1102 24-25 0.1527132850712718 1102 26-27 0.13927504605410945 1102 28-29 0.1563353503339755 1102 30-31 0.23742660190082887 1102 32-33 0.20041353442727683 1102 34-35 0.06405736134868789 1102 36-37 0.13879516336098874 1102 38-39 0.07561435657823523 1102 40-41 0.2111700328570265 1102 42-43 0.002034994881974228 1102 44-45 -0.23473585384406448 1102 46-47 -0.5882970739979854 1102 48-49 -0.11960100038297838 1102 50-51 0.07312486206448199 1102 52-53 0.13594399210180796 1102 54-55 0.03987969238255573 1102 56-57 0.018798955719375954 1102 58-59 0.020266122759231564 1102 60-61 -0.13868204505255477 1102 62-63 -0.4148217453832146 1102 64-65 -0.2600771377283877 1102 66-67 -0.003921883708386531 1102 68-69 0.016644215347774605 1102 70-71 0.22129565255185923 1102 72-73 0.23585613444745235 1102 74-75 0.23432324174372354 1102 76-77 0.1681415248735263 1102 78-79 0.08665962449383358 1102 80-81 0.04809847990564009 1102 82-83 -0.20166886423005792 1102 84-85 0.07670786724860079 1102 86-87 0.0499157555941494 1102 88-89 0.003908260493595606 1102 90-91 0.10757901245156631 1102 92-93 -0.002382935021913113 1102 94-95 0.10495118825172511 1102 96-97 -0.19568496656711432 1102 98-99 -0.2479377483731824 1102 100-101 -0.00752258601785627 1103 1 0.1079184330804992 1103 2 0.04935416954923255 1103 3 -0.029714421375985012 1103 4 0.14310549780788762 1103 5 0.12620458837418624 1103 6 0.13841215983453026 1103 7 0.10187607037147473 1103 8 0.16302605396483472 1103 9 0.2817425998425094 1103 10-11 0.29122824048597806 1103 12-13 0.2929020739935382 1103 14-15 0.3771469601390507 1103 16-17 0.4293797857396484 1103 18-19 0.467642861808919 1103 20-21 0.26977370510799403 1103 22-23 0.18153886465184854 1103 24-25 0.3507316149885398 1103 26-27 0.25967371644482284 1103 28-29 0.3180326199621746 1103 30-31 0.500902170861032 1103 32-33 0.3602321468334253 1103 34-35 0.39614692943806773 1103 36-37 0.43256872622538367 1103 38-39 0.3969311158148727 1103 40-41 0.23205084591522507 1103 42-43 0.3057105362304853 1103 44-45 0.37896538762020526 1103 46-47 0.6419148988575927 1103 48-49 0.3503542899115146 1103 50-51 0.653855085188205 1103 52-53 0.5995523095938182 1103 54-55 0.5759256977137355 1103 56-57 0.6060779294139209 1103 58-59 0.5345254390511869 1103 60-61 0.5319523867306302 1103 62-63 0.47775389473020624 1103 64-65 0.603567287364271 1103 66-67 0.4494796646704913 1103 68-69 0.4189556327835007 1103 70-71 0.5093737724078551 1103 72-73 0.4546570435093997 1103 74-75 0.4978115545629791 1103 76-77 0.056590278630309854 1103 78-79 0.27618045539344394 1103 80-81 0.3397703680145483 1103 82-83 0.02988121382608 1103 84-85 -0.0018999723486956555 1103 86-87 0.21869807986164602 1103 88-89 0.2831437351492454 1103 90-91 0.29548668319273474 1103 92-93 0.05974231587281764 1103 94-95 0.28902030244731947 1103 96-97 0.4114669933322119 1103 98-99 0.3774326200305467 1103 100-101 0.31968555306012547 1104 1 0.2618481801963384 1104 2 0.15934954059103035 1104 3 0.21286384874458264 1104 4 0.19601170875726126 1104 5 0.2158702901778895 1104 6 0.24232432485590039 1104 7 0.17323553380965961 1104 8 0.30150342471657865 1104 9 0.2105231971569239 1104 10-11 0.22054474401332413 1104 12-13 0.06430454899616933 1104 14-15 0.12622265850580305 1104 16-17 0.11331183740120565 1104 18-19 0.18827480400455698 1104 20-21 0.07009226881974229 1104 22-23 0.03920833319578776 1104 24-25 0.12618144840564582 1104 26-27 0.15265736807697294 1104 28-29 0.03324639637753535 1104 30-31 0.1442205776252692 1104 32-33 0.10508814252630572 1104 34-35 0.06322202385490527 1104 36-37 0.3090301757449083 1104 38-39 0.25098036157282166 1104 40-41 0.3161379577433081 1104 42-43 0.22888373674781093 1104 44-45 0.25969471673912636 1104 46-47 0.39676735826481035 1104 48-49 0.5588391901703744 1104 50-51 0.39937363777456625 1104 52-53 0.2085886121488798 1104 54-55 0.14478095436265193 1104 56-57 0.05304010109065871 1104 58-59 0.21013182264282904 1104 60-61 0.3390187000336269 1104 62-63 0.3115613817224343 1104 64-65 0.4717646558114623 1104 66-67 0.34709575119426006 1104 68-69 0.34383191245203903 1104 70-71 0.45229851905682494 1104 72-73 0.24913179582040357 1104 74-75 0.3132864797149111 1104 76-77 0.33422446378466475 1104 78-79 0.14330104472141159 1104 80-81 0.3210389806978995 1104 82-83 0.2029898473298033 1104 84-85 0.10119417827720412 1104 86-87 0.3094708576140377 1104 88-89 0.34889033842765826 1104 90-91 0.20987529317419273 1104 92-93 0.33319454522278846 1104 94-95 0.12485519366680364 1104 96-97 0.13514252521540726 1104 98-99 0.38798535340056617 1104 100-101 0.4193958955036976 1105 1 0.14434628354091217 1105 2 0.09035899159464122 1105 3 0.056327801065798155 1105 4 0.0010411431657217918 1105 5 -0.07948596766247817 1105 6 -0.08541773374724215 1105 7 0.10259482442778278 1105 8 0.06842949584746805 1105 9 0.17385296636832948 1105 10-11 0.13541601934220893 1105 12-13 0.09828391450762552 1105 14-15 0.13118389751057435 1105 16-17 0.17074892503651284 1105 18-19 0.09507301576952187 1105 20-21 0.033633453137234426 1105 22-23 0.024369050772335754 1105 24-25 -0.026242294820413292 1105 26-27 0.1115338558172354 1105 28-29 0.15305047257303528 1105 30-31 0.1037542295780085 1105 32-33 0.18918224282874263 1105 34-35 0.1439325469950461 1105 36-37 0.10702350962080942 1105 38-39 0.0822781229113545 1105 40-41 0.12176375113462257 1105 42-43 0.2045957893733359 1105 44-45 0.2601825617737248 1105 46-47 0.3966826986758747 1105 48-49 0.03418968554590407 1105 50-51 0.02078484296227856 1105 52-53 0.20231912524211992 1105 54-55 0.1716788492856054 1105 56-57 0.2938320845291855 1105 58-59 0.18776438505938842 1105 60-61 0.30215015064995043 1105 62-63 0.3072357912334951 1105 64-65 0.17247710600963728 1105 66-67 0.23864401136955138 1105 68-69 0.2321284960400547 1105 70-71 0.1334915550806457 1105 72-73 0.2977647539386936 1105 74-75 0.2692017527724886 1105 76-77 0.07849871595049507 1105 78-79 0.17424669700717033 1105 80-81 0.22469028796356127 1105 82-83 0.1478203270269347 1105 84-85 0.2355896386523071 1105 86-87 0.2770803795259056 1105 88-89 0.1101259755308206 1105 90-91 0.25751525511947193 1105 92-93 0.2589476179916872 1105 94-95 0.22698272187809465 1105 96-97 0.13653823910154017 1105 98-99 0.17716613857381702 1105 100-101 0.204009740199659 1106 1 0.10143174705589075 1106 2 0.1691456974691974 1106 3 0.21040332462905553 1106 4 0.29122609041964864 1106 5 0.28551561821408455 1106 6 0.05753694243826857 1106 7 0.08750032589629342 1106 8 0.21129278253631867 1106 9 0.2826216989752126 1106 10-11 0.1147091305453074 1106 12-13 0.2928526008821706 1106 14-15 0.2579966798548483 1106 16-17 0.41922352682021113 1106 18-19 0.4670054197971112 1106 20-21 0.28625776525207414 1106 22-23 0.4031803063744164 1106 24-25 0.4591138823031784 1106 26-27 0.3850128926794554 1106 28-29 0.3862760200588369 1106 30-31 0.39741108172950845 1106 32-33 0.3554703598646469 1106 34-35 0.4634665143206078 1106 36-37 0.38685366851309766 1106 38-39 0.2928368352871118 1106 40-41 0.42880065277981316 1106 42-43 0.34463540940806325 1106 44-45 0.41958376345601067 1106 46-47 0.5983300391519251 1106 48-49 0.35280796595396424 1106 50-51 0.16139878326522705 1106 52-53 0.1418622852758844 1106 54-55 0.3240923383753085 1106 56-57 0.38791577938862076 1106 58-59 0.34693576903683976 1106 60-61 0.4500353797777521 1106 62-63 0.3944088273896895 1106 64-65 0.5580529763154018 1106 66-67 0.5318914027370027 1106 68-69 0.42770381084103093 1106 70-71 0.43454378865786936 1106 72-73 0.45379126406053416 1106 74-75 0.3954393188730805 1106 76-77 0.43831529185485607 1106 78-79 0.4321415847504113 1106 80-81 0.37220189532560966 1106 82-83 0.327040505827803 1106 84-85 0.36779260934853397 1106 86-87 0.43524379111373435 1106 88-89 0.4132075935191395 1106 90-91 0.5344912841920859 1106 92-93 0.5074930018365293 1106 94-95 0.427414671646126 1106 96-97 0.4399397203281694 1106 98-99 0.4364502362638163 1106 100-101 0.5105014407789952 1107 1 0.058310092251439016 1107 2 0.07836353222453596 1107 3 0.04689673444646303 1107 4 0.1188994679682267 1107 5 0.16652854932115702 1107 6 0.1668315756414671 1107 7 0.19117654633478054 1107 8 0.2753453827364041 1107 9 0.2845248184069007 1107 10-11 0.2664786947814832 1107 12-13 0.23151573526934754 1107 14-15 0.2904120247236648 1107 16-17 0.15216029272979625 1107 18-19 0.21756456639120358 1107 20-21 0.14950010814366976 1107 22-23 0.2657683467003409 1107 24-25 0.26595315460420466 1107 26-27 0.19654849111789474 1107 28-29 0.21704154563979472 1107 30-31 0.20887847102017787 1107 32-33 0.2986327717401096 1107 34-35 0.3131310263641538 1107 36-37 0.3670645947764868 1107 38-39 0.313050321498757 1107 40-41 0.2384929200518826 1107 42-43 0.3053427298495919 1107 44-45 0.3372047967993339 1107 46-47 0.3517548120407241 1107 48-49 0.23211819463144678 1107 50-51 0.10041391053844251 1107 52-53 0.1139723226309215 1107 54-55 0.19044367106416615 1107 56-57 0.32187921928540675 1107 58-59 0.30754793350678256 1107 60-61 0.20509633012486717 1107 62-63 0.13219433740885478 1107 64-65 0.4081191644513922 1107 66-67 0.23580054478382095 1107 68-69 0.385222143102105 1107 70-71 0.33035221021398087 1107 72-73 0.40261335102466234 1107 74-75 0.3582190184833607 1107 76-77 0.3562942127019326 1107 78-79 0.42457121143584686 1107 80-81 0.3667140062387446 1107 82-83 0.3468784206825717 1107 84-85 0.44428285291414227 1107 86-87 0.36029625321133807 1107 88-89 0.36899375026208503 1107 90-91 0.4260488442919268 1107 92-93 0.3399789137002145 1107 94-95 0.3543214120572671 1107 96-97 0.3058683247184817 1107 98-99 0.33071847842145274 1107 100-101 0.2808665028745807 1108 1 -0.01185721279011176 1108 2 0.012669000053243451 1108 3 -0.03506313350172974 1108 4 -0.10777599385529157 1108 5 -0.04205046240605981 1108 6 -0.027053404865021946 1108 7 -0.059026478659852444 1108 8 0.033779865559395716 1108 9 -0.09731463471688073 1108 10-11 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-1.0197166495300323 2316 80-81 -1.095634389499736 2316 82-83 -1.1964234146083612 2316 84-85 -1.6433991425496899 2316 86-87 -1.20386323306478 2316 88-89 -1.1011730636706005 2316 90-91 -1.0939933094312764 2316 92-93 -1.2790738375478021 2316 94-95 -1.1185400884802092 2316 96-97 -0.9402663645877567 2316 98-99 -0.7319256687395068 2316 100-101 -1.33561594057808 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 3.0 7 49.0 8 213.0 9 263.0 10 320.0 11 393.0 12 545.0 13 666.0 14 841.0 15 1137.0 16 1720.0 17 2498.0 18 3149.0 19 4107.0 20 5206.0 21 6544.0 22 6928.0 23 6042.0 24 5085.0 25 5212.0 26 5846.0 27 6734.0 28 8127.0 29 9687.0 30 11851.0 31 14661.0 32 18838.0 33 25044.0 34 36029.0 35 53034.0 36 88410.0 37 203106.0 38 467693.0 39 183987.0 40 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.41337058891432 28.164743907490276 15.860980776935039 20.560904726660375 2 11.997442969140131 24.802436301020865 43.230438841095285 19.96968188874372 3 13.631866397299103 33.93405008577839 33.96561924212019 18.46846427480231 4 8.842697713638419 21.76047979220744 48.39228295819936 21.004539535954784 5 8.369752339467759 44.25262779959201 38.55635934698979 8.821260513950433 6 21.329028217411174 47.74844694212638 21.327758687814207 9.594766152648239 7 21.693214380618222 35.283579007587726 30.144307178291974 12.878899433502081 8 21.91382368672268 38.645880584686836 24.605712138548302 14.83458359004218 9 24.759298234924245 17.57789326610525 26.584374886281797 31.07843361268871 10-11 19.522391015747495 29.458027345989958 35.16531463038817 15.854267007874382 12-13 20.741150091419826 29.661617595597296 32.05330065548923 17.54393165749365 14-15 20.430064752625164 30.797001937796686 29.39534734127439 19.377585968303755 16-17 17.62954652955342 33.884931224275945 30.31123968765706 18.17428255851358 18-19 17.13893173502121 33.02027843719278 32.86742653243751 16.973363295348495 20-21 18.19206467583955 32.89687389410615 31.83221748028078 17.07884394977352 22-23 19.26706081081081 31.914527027027027 31.130152027027023 17.68826013513514 24-25 18.824324324324323 32.45173141891892 30.680532094594593 18.043412162162163 26-27 18.435346283783787 31.565498310810813 32.476351351351354 17.522804054054053 28-29 18.406165540540538 30.07804054054054 33.36528716216216 18.150506756756755 30-31 19.187964527027027 31.879054054054055 30.968116554054053 17.964864864864865 32-33 18.192187500000003 31.823733108108108 31.97495777027027 18.00912162162162 34-35 18.328125 31.71896114864865 32.405616554054056 17.5472972972973 36-37 19.51288006756757 30.098310810810812 31.27124155405405 19.117567567567566 38-39 17.938893581081082 32.88568412162162 32.09940878378379 17.076013513513512 40-41 17.55181587837838 31.213766891891893 33.58961148648649 17.644805743243243 42-43 18.08741554054054 31.222086148648646 30.613386824324323 20.077111486486487 44-45 18.530010547229512 31.775326249406877 31.33372973549037 18.36093346787324 46-47 18.69800443684187 31.30686237749382 31.88051166723517 18.114621518429143 48-49 17.227882472685955 31.613024850622363 31.761261230797324 19.397831445894358 50-51 17.220050363825607 31.379984879865074 30.574239735697674 20.825725020611642 52-53 17.934903652926547 31.068238535871036 31.841719899331622 19.155137911870796 54-55 18.321887700932557 31.159973423140126 31.13900948787135 19.379129388055965 56-57 18.989179565601393 31.31102163960043 30.06844528288166 19.63135351191651 58-59 18.251184747978712 30.399717968476892 30.628741768268323 20.720355515276072 60-61 19.997944565002708 32.21259964736179 29.69515699759311 18.09429879004239 62-63 18.015138558450243 32.217739545673915 32.02351217942748 17.743609716448365 64-65 18.023749011515054 31.636888778000028 30.69086111310805 19.648501097376865 66-67 18.64478934723579 31.83751135627177 30.06751829369084 19.4501810028016 68-69 18.43977717987787 31.728530141427193 30.409002433501485 19.422690245193454 70-71 18.890111878156453 30.650633108292034 31.970869748261464 18.48838526529004 72-73 18.74755669420356 31.309307616267546 30.513883471060062 19.429252218468836 74-75 18.23853409659914 31.43307721976371 29.802171721272654 20.5262169623645 76-77 17.41383652057618 31.985584929776262 32.08999473083898 18.510583818808577 78-79 17.874595330681707 31.761387304749032 30.440986473313497 19.92303089125576 80-81 18.345033036444914 31.999110687306835 30.012993095146314 19.642863181101937 82-83 18.598834722299713 31.82652038136976 30.85978118073133 18.7148637155992 84-85 18.183993361321413 31.431378409329657 30.7289145650596 19.65571366428933 86-87 16.846345750482953 32.40638958516383 32.05134219409229 18.695922470260925 88-89 18.597081550869955 30.165029336669992 31.192066417544513 20.04582269491554 90-91 17.8187187069529 30.808171348697034 32.464435539176925 18.908674405173134 92-93 19.494676463496656 31.632720396949278 30.646311595030518 18.226291544523544 94-95 18.568470871610458 29.460683736106485 31.5432523727446 20.42759301953846 96-97 19.156125634311245 29.77992226583057 31.59883419880757 19.465117901050615 98-99 17.99141923314098 30.695493512438137 31.3184888010891 19.994598453331776 100-101 17.812669033130494 30.379141311697094 32.06909229208925 19.739097363083165 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 183.0 1 125.0 2 114.0 3 173.0 4 141.5 5 147.5 6 264.0 7 480.0 8 724.5 9 845.0 10 725.0 11 569.5 12 598.0 13 647.5 14 675.0 15 747.0 16 800.5 17 827.0 18 910.0 19 955.5 20 1011.0 21 1197.5 22 1445.5 23 1630.5 24 1805.0 25 2306.0 26 3395.5 27 4841.5 28 5831.5 29 8266.0 30 15375.0 31 22839.0 32 40363.5 33 63698.0 34 83850.5 35 103397.5 36 125713.5 37 134839.0 38 114463.5 39 93812.5 40 72392.5 41 52076.0 42 40874.0 43 33111.0 44 29768.0 45 25813.0 46 19656.0 47 15351.5 48 17062.5 49 14841.0 50 9423.0 51 5294.0 52 1950.5 53 1041.5 54 808.0 55 665.5 56 530.0 57 439.5 58 375.0 59 302.5 60 272.5 61 235.5 62 195.5 63 165.5 64 128.0 65 100.0 66 76.0 67 53.5 68 39.5 69 33.5 70 32.5 71 32.5 72 30.5 73 21.0 74 15.0 75 24.5 76 26.5 77 13.5 78 13.5 79 22.5 80 23.5 81 13.0 82 5.0 83 1.0 84 2.0 85 2.0 86 0.0 87 1.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4925675675675676 2 0.3806587837837838 3 0.20836148648648647 4 0.2383445945945946 5 0.25920608108108106 6 0.20777027027027029 7 0.19881756756756755 8 0.20067567567567568 9 0.19856418918918917 10-11 0.21203547297297298 12-13 0.19961993243243245 14-15 0.21144425675675674 16-17 0.09573479729729731 18-19 0.04235641891891892 20-21 2.533783783783784E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.1434966216216216 46-47 0.21359797297297298 48-49 0.09222972972972973 50-51 0.06836993243243243 52-53 0.07664695945945946 54-55 0.08581081081081081 56-57 0.11625844594594595 58-59 0.09742398648648648 60-61 0.14936655405405405 62-63 0.24577702702702703 64-65 0.13809121621621623 66-67 0.10840371621621621 68-69 0.1133445945945946 70-71 0.09341216216216215 72-73 0.07774493243243243 74-75 0.14459459459459462 76-77 0.21938344594594597 78-79 0.14421452702702703 80-81 0.08986486486486486 82-83 0.09341216216216215 84-85 0.10426520270270272 86-87 0.07757601351351351 88-89 0.09966216216216216 90-91 0.12069256756756758 92-93 0.16765202702702703 94-95 0.13631756756756758 96-97 0.09480574324324324 98-99 0.08492398648648648 100-101 0.06756756756756757 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1184000.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 11.2730730108881 #Duplication Level Percentage of deduplicated Percentage of total 1 74.49880092317032 8.398304220305167 2 8.903168689179877 2.0073214132275528 3 3.467319510505894 1.1726203798202919 4 1.839015119535481 0.8292540684260234 5 1.2328902016517251 0.6949230628814226 6 0.9337670399597311 0.6315854409976149 7 0.6813727095570676 0.5376815011724433 8 0.5712022740567843 0.5151363951541954 9 0.46190311863295536 0.4686360822275591 >10 4.759036069883157 11.900021666650723 >50 1.1453017910423013 9.24084403686178 >100 1.2387343055746995 30.061474499193093 >500 0.17480922073803545 13.729224874907327 >1k 0.090418562450708 18.019098000787846 >5k 0.0022604640612677 1.7938743573869522 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 9005 0.7605574324324325 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 6098 0.5150337837837837 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 6016 0.5081081081081081 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 4345 0.3669763513513513 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 4307 0.3637668918918919 No Hit ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC 3837 0.324070945945946 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 3582 0.30253378378378376 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 3454 0.29172297297297295 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 3435 0.2901182432432432 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3148 0.26587837837837835 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 3095 0.26140202702702703 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 3036 0.25641891891891894 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 2992 0.25270270270270273 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2988 0.25236486486486487 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2941 0.24839527027027025 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 2755 0.23268581081081083 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 2722 0.22989864864864865 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 2668 0.22533783783783784 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 2656 0.2243243243243243 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 2621 0.22136824324324325 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 2566 0.216722972972973 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 2410 0.2035472972972973 No Hit CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC 2395 0.20228040540540543 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 2368 0.2 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 2330 0.19679054054054052 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 2307 0.19484797297297296 No Hit TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC 2300 0.19425675675675677 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 2209 0.18657094594594595 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 2207 0.18640202702702702 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 2139 0.18065878378378378 No Hit CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC 2134 0.1802364864864865 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 2110 0.17820945945945946 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 2104 0.17770270270270272 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 2069 0.1747466216216216 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 2061 0.17407094594594594 No Hit ATATAGGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATT 2054 0.17347972972972975 No Hit ATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTC 2049 0.17305743243243243 No Hit GTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTC 2044 0.17263513513513512 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 1979 0.1671452702702703 No Hit CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG 1951 0.16478040540540542 No Hit GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG 1950 0.16469594594594594 No Hit ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG 1926 0.16266891891891894 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 1911 0.16140202702702702 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1873 0.15819256756756755 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 1861 0.15717905405405405 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 1858 0.1569256756756757 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 1818 0.1535472972972973 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1811 0.15295608108108108 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1779 0.15025337837837838 No Hit CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT 1752 0.14797297297297296 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 1700 0.14358108108108109 No Hit CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA 1648 0.13918918918918918 No Hit TTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGT 1642 0.13868243243243245 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 1620 0.13682432432432431 No Hit CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC 1600 0.13513513513513514 No Hit GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA 1598 0.1349662162162162 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 1576 0.1331081081081081 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 1554 0.13125 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 1554 0.13125 No Hit CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA 1546 0.13057432432432434 No Hit ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG 1533 0.12947635135135135 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1524 0.1287162162162162 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 1515 0.12795608108108109 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1501 0.12677364864864865 No Hit GCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGG 1488 0.12567567567567567 No Hit AAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCA 1488 0.12567567567567567 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1488 0.12567567567567567 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 1487 0.12559121621621622 No Hit ACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGGTAAAA 1485 0.12542229729729729 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1478 0.12483108108108107 No Hit AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT 1447 0.12221283783783785 No Hit GGCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTG 1445 0.12204391891891891 No Hit CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC 1439 0.12153716216216216 No Hit ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA 1416 0.11959459459459461 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 1408 0.11891891891891893 No Hit GATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAG 1390 0.11739864864864864 No Hit GTTTATATTCTTATCCTCCCAGGATTTGGAATTATTTCACATGTAGTTAC 1378 0.11638513513513513 No Hit ATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATG 1375 0.11613175675675676 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 1363 0.11511824324324324 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 1362 0.11503378378378379 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1351 0.11410472972972974 No Hit TAGTATAGCTGGAGATCATTTAATATTACCTCCGTGTAGGGTTGCAAGTC 1333 0.11258445945945947 No Hit GAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAA 1314 0.11097972972972973 No Hit TAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCA 1308 0.11047297297297298 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 1304 0.11013513513513515 No Hit TCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAA 1292 0.10912162162162163 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 1288 0.10878378378378378 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1277 0.10785472972972973 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 1271 0.10734797297297298 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 1270 0.10726351351351353 No Hit TCCTTTTGGTGTGTGGATTAGCATTATTTGTTTGATAATAAGTTTAACTA 1261 0.10650337837837837 No Hit TTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAAC 1260 0.10641891891891891 No Hit ATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC 1257 0.10616554054054053 No Hit CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT 1252 0.10574324324324325 No Hit CTACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTC 1250 0.10557432432432433 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 1215 0.10261824324324323 No Hit CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC 1196 0.10101351351351352 No Hit ACTTACTACTCCGGAAAAAAAGAACCTTTCGGCTATATAGGAATAGTATG 1191 0.10059121621621622 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.445945945945946E-5 0.0 10-11 0.0 0.0 0.0 5.067567567567568E-4 0.0 12-13 0.0 0.0 0.0 0.0013935810810810812 0.0 14-15 0.0 0.0 0.0 0.0025760135135135137 0.0 16-17 0.0 0.0 0.0 0.006123310810810811 0.0 18-19 0.0 0.0 0.0 0.010135135135135136 0.0 20-21 0.0 0.0 0.0 0.016849662162162164 0.0 22-23 0.0 0.0 0.0 0.03289695945945946 0.0 24-25 0.0 0.0 0.0 0.052998310810810814 0.0 26-27 0.0 0.0 0.0 0.0613597972972973 0.0 28-29 0.0 0.0 0.0 0.06879222972972973 0.0 30-31 0.0 0.0 0.0 0.08133445945945947 0.0 32-33 0.0 0.0 0.0 0.09894425675675675 0.0 34-35 0.0 0.0 0.0 0.12381756756756757 0.0 36-37 0.0 0.0 0.0 0.15515202702702702 0.0 38-39 0.0 0.0 0.0 0.2007179054054054 0.0 40-41 0.0 0.0 0.0 0.26203547297297297 0.0 42-43 0.0 0.0 0.0 0.3171875 0.0 44-45 0.0 0.0 0.0 0.3774070945945946 0.0 46-47 0.0 0.0 0.0 0.45434966216216216 0.0 48-49 0.0 0.0 0.0 0.5347128378378379 0.0 50-51 0.0 0.0 0.0 0.6236908783783783 0.0 52-53 0.0 0.0 0.0 0.7118665540540541 0.0 54-55 0.0 0.0 0.0 0.8076435810810811 0.0 56-57 0.0 0.0 0.0 0.916554054054054 0.0 58-59 0.0 0.0 0.0 1.039695945945946 0.0 60-61 0.0 0.0 0.0 1.169214527027027 0.0 62-63 0.0 0.0 0.0 1.3020692567567567 0.0 64-65 0.0 0.0 0.0 1.4445523648648648 0.0 66-67 0.0 0.0 0.0 1.6069256756756758 0.0 68-69 0.0 0.0 0.0 1.791722972972973 0.0 70-71 0.0 0.0 0.0 2.0145692567567566 0.0 72-73 0.0 0.0 0.0 2.2440033783783786 0.0 74-75 0.0 0.0 0.0 2.469974662162162 0.0 76-77 0.0 0.0 0.0 2.72191722972973 0.0 78-79 0.0 0.0 0.0 3.015456081081081 0.0 80-81 0.0 0.0 0.0 3.3559966216216215 0.0 82-83 0.0 0.0 0.0 3.7103885135135135 0.0 84-85 0.0 0.0 0.0 4.057981418918919 0.0 86-87 0.0 0.0 0.0 4.427533783783783 0.0 88-89 0.0 0.0 0.0 4.841638513513513 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGTATT 45 2.5465852E-11 84.65389 1 CAGAGTT 20 0.0020718356 71.269554 9 CAGTGTA 20 0.0020725313 71.26352 7 CAGATCC 30 1.297844E-4 63.366802 4 GACATAT 450 0.0 50.792336 1 TAACTTT 1315 0.0 48.050938 6 TGGGTAT 155 0.0 45.976463 6 GTATGGT 90 1.1707016E-8 42.326946 1 GGGACAT 45 9.616202E-4 42.230236 7 ACATATA 545 0.0 41.901306 2 AACTTTA 1545 0.0 40.897724 7 CATGGGT 420 0.0 38.4727 4 ACTTTAT 1660 0.0 38.353893 8 GGCATTC 250 0.0 38.00721 7 CATTCCG 255 0.0 37.26512 9 ATGGGTA 335 0.0 36.88515 5 CATGCCA 65 1.3260654E-4 36.54849 9 GCATGCC 65 1.3260654E-4 36.54849 8 CTTAATA 2635 0.0 36.471497 2 CCTTTAG 240 0.0 35.71336 1 >>END_MODULE