FastQCFastQC Report
Fri 10 Feb 2017
SRR2031629_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031629_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1184000
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC83430.7046452702702702No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC61950.5232263513513514No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT57670.48707770270270273No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA43310.36579391891891894No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC40860.34510135135135134No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA38560.3256756756756757No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC36730.3102195945945946No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC34370.29028716216216216No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA34330.2899493243243243No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32230.27221283783783784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31600.2668918918918919No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30950.26140202702702703No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT30460.2572635135135135No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC28940.2444256756756757No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC28540.2410472972972973No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT28460.24037162162162162No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC25670.21680743243243242No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT25290.21359797297297298No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC24810.2095439189189189No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT24790.209375No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT24570.20751689189189187No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA24420.20625000000000002No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA24040.20304054054054052No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA23760.20067567567567568No Hit
ATATAGGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATT23760.20067567567567568No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA22990.1941722972972973No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT22960.19391891891891894No Hit
CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC22780.19239864864864864No Hit
TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC22680.19155405405405407No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT22520.1902027027027027No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT21680.18310810810810813No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG21360.1804054054054054No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC21250.17947635135135137No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC20770.1754222972972973No Hit
ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG20510.17322635135135137No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT20410.17238175675675674No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC20080.16959459459459458No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA19900.16807432432432431No Hit
ATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTC19560.1652027027027027No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA19500.16469594594594594No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG19380.16368243243243244No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC19360.1635135135135135No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT19180.16199324324324324No Hit
GTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTC19050.16089527027027029No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG19010.16055743243243242No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT18590.15701013513513515No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT18400.1554054054054054No Hit
TTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGT18360.15506756756756757No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA18160.15337837837837837No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT17790.15025337837837838No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG17380.14679054054054055No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17180.14510135135135135No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG17100.14442567567567569No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC16270.13741554054054053No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA16260.13733108108108108No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA16190.13673986486486486No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA15710.13268581081081082No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC15690.1325168918918919No Hit
CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA15670.132347972972973No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG15580.13158783783783784No Hit
AAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCA15470.1306587837837838No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT15230.12863175675675675No Hit
GGCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTG15190.12829391891891892No Hit
CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC15040.12702702702702703No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15000.1266891891891892No Hit
GCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGG14890.12576013513513512No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC14880.12567567567567567No Hit
ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA14460.12212837837837838No Hit
ACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGGTAAAA14420.12179054054054055No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC14380.12145270270270271No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT14340.12111486486486486No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA14230.12018581081081081No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA14210.1200168918918919No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC14030.11849662162162161No Hit
ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT13990.11815878378378378No Hit
TCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAA13970.11798986486486486No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT13800.11655405405405406No Hit
CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT13700.11570945945945947No Hit
GATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAG13650.11528716216216216No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG13490.1139358108108108No Hit
AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT13420.1133445945945946No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA13230.11173986486486485No Hit
TCCTTTTGGTGTGTGGATTAGCATTATTTGTTTGATAATAAGTTTAACTA13150.11106418918918919No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG13130.11089527027027027No Hit
GTTTATATTCTTATCCTCCCAGGATTTGGAATTATTTCACATGTAGTTAC13090.11055743243243243No Hit
AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA13060.11030405405405405No Hit
ATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATG13010.10988175675675675No Hit
TAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCA12900.1089527027027027No Hit
CATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCC12760.10777027027027027No Hit
TTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAAC12720.10743243243243243No Hit
ACTTACTACTCCGGAAAAAAAGAACCTTTCGGCTATATAGGAATAGTATG12600.10641891891891891No Hit
TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC12590.10633445945945946No Hit
TAGTATAGCTGGAGATCATTTAATATTACCTCCGTGTAGGGTTGCAAGTC12410.1048141891891892No Hit
CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC12310.1039695945945946No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT12230.10329391891891891No Hit
TTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTAT12220.10320945945945946No Hit
GAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAA12170.10278716216216216No Hit
TTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGT12130.10244932432432433No Hit
ATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC12120.10236486486486487No Hit
ATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTAT11990.1012668918918919No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGCGA352.7897288E-454.2788126
GACATAT3350.052.4892651
TAACTTT12050.046.902756
ACATATA3900.045.062182
TCAGGCC554.964652E-543.1799773
GGGATGC2200.043.1763277
ATGGGCG901.1979864E-842.2168545
ACCTGGG1051.027729E-940.7125473
GGTAACA704.3140735E-640.709118
ATGGGTA3300.040.2979055
ATGTATT608.2661965E-539.6033861
TACTAAG608.2661965E-539.6033861
TACCTGG1208.731149E-1139.5816422
CATGGGT4600.038.204894
AACTTTA15650.036.720577
GTATGGT651.3285427E-436.5369034
GGGAGGT1302.055458E-1036.5338177
ACTTTAT15550.036.345868
CTATAAC18050.036.051093
CATTCCG2650.035.8444989
Produced by FastQC (version 0.11.5)