##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031629_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1184000 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.596184966216214 34.0 31.0 34.0 31.0 34.0 2 32.80162753378379 34.0 31.0 34.0 31.0 34.0 3 32.89410810810811 34.0 31.0 34.0 31.0 34.0 4 36.31901182432433 37.0 37.0 37.0 35.0 37.0 5 36.08932516891892 37.0 37.0 37.0 35.0 37.0 6 36.15488006756757 37.0 37.0 37.0 35.0 37.0 7 36.089418918918916 37.0 37.0 37.0 35.0 37.0 8 36.19404307432433 37.0 37.0 37.0 35.0 37.0 9 38.06898310810811 39.0 39.0 39.0 37.0 39.0 10-11 38.04481967905406 39.0 39.0 39.0 36.0 39.0 12-13 38.09189527027027 39.0 39.0 39.0 37.0 39.0 14-15 39.534480996621625 41.0 39.5 41.0 37.0 41.0 16-17 39.50170692567568 41.0 39.0 41.0 37.0 41.0 18-19 39.57742567567568 41.0 40.0 41.0 37.0 41.0 20-21 39.62131461148648 41.0 40.0 41.0 37.0 41.0 22-23 39.54449873310811 41.0 39.5 41.0 37.0 41.0 24-25 39.48240160472973 41.0 39.0 41.0 37.0 41.0 26-27 39.38119425675676 41.0 39.0 41.0 36.0 41.0 28-29 39.325540962837835 41.0 39.0 41.0 36.0 41.0 30-31 39.19956587837838 41.0 39.0 41.0 36.0 41.0 32-33 39.047966638513515 40.5 39.0 41.0 35.0 41.0 34-35 38.98992483108108 40.0 39.0 41.0 35.0 41.0 36-37 38.86133488175676 40.0 38.0 41.0 35.0 41.0 38-39 38.731397804054055 40.0 38.0 41.0 35.0 41.0 40-41 38.69740456081081 40.0 38.0 41.0 35.0 41.0 42-43 38.600350506756754 40.0 38.0 41.0 35.0 41.0 44-45 38.52749831081081 40.0 38.0 41.0 35.0 41.0 46-47 38.51940793918919 40.0 38.5 41.0 34.5 41.0 48-49 38.616119510135135 41.0 39.0 41.0 35.0 41.0 50-51 38.57001309121622 41.0 39.0 41.0 35.0 41.0 52-53 38.569681165540544 41.0 39.0 41.0 35.0 41.0 54-55 38.508002956081086 41.0 39.0 41.0 35.0 41.0 56-57 38.44172930743243 41.0 39.0 41.0 34.5 41.0 58-59 38.31504349662163 40.0 38.5 41.0 34.0 41.0 60-61 38.11250126689189 40.0 38.0 41.0 34.0 41.0 62-63 37.95053420608108 40.0 38.0 41.0 34.0 41.0 64-65 37.73015836148649 40.0 37.5 41.0 33.0 41.0 66-67 37.496855152027024 39.5 37.0 41.0 33.0 41.0 68-69 37.207944256756754 39.0 36.5 41.0 33.0 41.0 70-71 36.834003800675674 39.0 36.0 40.5 32.0 41.0 72-73 36.4763285472973 38.0 36.0 40.0 32.0 41.0 74-75 36.05594467905405 37.0 35.0 39.5 32.0 41.0 76-77 35.37040498310811 37.0 35.0 39.0 31.0 40.0 78-79 35.237671875000004 36.0 35.0 39.0 31.0 40.0 80-81 34.865648226351354 36.0 35.0 37.5 31.0 39.0 82-83 34.49971706081081 35.5 35.0 37.0 31.0 39.0 84-85 34.11003547297297 35.0 34.5 36.5 30.5 38.0 86-87 33.84822086148648 35.0 34.0 36.0 30.0 37.0 88-89 33.56705996621622 35.0 34.0 36.0 30.0 37.0 90-91 33.38650886824324 35.0 34.0 36.0 30.0 36.5 92-93 33.24435388513514 35.0 34.0 35.0 30.0 36.0 94-95 33.08190878378379 35.0 34.0 35.0 29.0 36.0 96-97 32.965151182432436 35.0 34.0 35.0 29.0 36.0 98-99 32.911982263513515 35.0 34.0 35.0 29.0 36.0 100-101 32.524969594594594 35.0 33.5 35.0 27.5 35.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17916132418816488 1101 2 0.25441803361166393 1101 3 0.3050690858519758 1101 4 0.12157164460479919 1101 5 0.24528950310341457 1101 6 0.2449591461192071 1101 7 0.29837681338732835 1101 8 0.13051938814117392 1101 9 0.22307028138590113 1101 10-11 0.20602715992365006 1101 12-13 0.2695423297997763 1101 14-15 0.2572615151211295 1101 16-17 0.28403004101750184 1101 18-19 0.15651969373488583 1101 20-21 0.14288117357136088 1101 22-23 0.1924958108124244 1101 24-25 0.21062994202632268 1101 26-27 0.3023236491451513 1101 28-29 0.21035146271793792 1101 30-31 0.22172582801488971 1101 32-33 0.2337347369333287 1101 34-35 0.28136145235286136 1101 36-37 0.1513301546106831 1101 38-39 0.10821191940932806 1101 40-41 0.1801758183069211 1101 42-43 0.17864010833260124 1101 44-45 0.11642431045748936 1101 46-47 0.27531424488145007 1101 48-49 0.20686680666062784 1101 50-51 0.36705146122678656 1101 52-53 0.23257408197942908 1101 54-55 0.21753559839089576 1101 56-57 0.21188940666394274 1101 58-59 0.26782826463146847 1101 60-61 0.20005476499614616 1101 62-63 0.0595310978164747 1101 64-65 0.18773037071979815 1101 66-67 0.1672090616196158 1101 68-69 0.1247549754442332 1101 70-71 0.0899823729732745 1101 72-73 0.23700143031783227 1101 74-75 0.051551369137207814 1101 76-77 0.21224897410447596 1101 78-79 0.4124964653092178 1101 80-81 0.37510288439523976 1101 82-83 0.43949784345597465 1101 84-85 0.37448442250283165 1101 86-87 0.295106633016637 1101 88-89 0.2351103717343932 1101 90-91 0.32931258144598985 1101 92-93 0.04617477264620362 1101 94-95 0.3003692777117166 1101 96-97 0.22165753055801218 1101 98-99 0.27874375680413976 1101 100-101 0.40970506648081084 1102 1 0.0406209986081052 1102 2 0.09928971844558276 1102 3 0.03285783073796722 1102 4 0.028320707632289555 1102 5 0.006832146081130475 1102 6 0.05692950631718219 1102 7 0.2069168528689147 1102 8 0.03160370136178159 1102 9 0.11901626599380677 1102 10-11 0.06036870428322061 1102 12-13 0.033874432909662744 1102 14-15 -0.015636376333112878 1102 16-17 0.10521563309829673 1102 18-19 0.10398915272266862 1102 20-21 0.11051199209909868 1102 22-23 0.10317872787248206 1102 24-25 0.07777725413114922 1102 26-27 0.04953275852059846 1102 28-29 -0.29189154932681305 1102 30-31 -0.23366855873302939 1102 32-33 0.05301491316999574 1102 34-35 0.012137065318846396 1102 36-37 0.0028107142193007917 1102 38-39 -0.018503956195054627 1102 40-41 -0.11024274376214294 1102 42-43 -0.008545540967894283 1102 44-45 -0.0412989178777039 1102 46-47 0.00458211888368254 1102 48-49 -0.06696662680717225 1102 50-51 0.08727004046176745 1102 52-53 -0.07251274890776216 1102 54-55 -0.2644716685103745 1102 56-57 -0.194115800328305 1102 58-59 -0.06529621681504238 1102 60-61 0.10123062972878927 1102 62-63 0.06122766177379191 1102 64-65 0.1370651917652097 1102 66-67 0.047379576310774496 1102 68-69 -0.16112606480982095 1102 70-71 -0.11975355813421373 1102 72-73 0.012199553511834438 1102 74-75 0.12654922339864783 1102 76-77 -0.09667729831848959 1102 78-79 -0.18654224381446483 1102 80-81 -0.2206971899903607 1102 82-83 -0.42402173998464576 1102 84-85 -0.1716346516825098 1102 86-87 -0.14688117918832688 1102 88-89 -0.14404163238542367 1102 90-91 -0.03473056220406079 1102 92-93 -0.02530693135631168 1102 94-95 -0.011675468089784147 1102 96-97 0.06993664823031764 1102 98-99 0.012749421553955642 1102 100-101 0.027311566638161366 1103 1 0.2315516189154394 1103 2 0.20412707494442373 1103 3 0.1560760799441283 1103 4 0.11341039254038066 1103 5 0.14755620967298455 1103 6 0.08024439645449633 1103 7 0.22714348116240757 1103 8 0.19654428311894634 1103 9 0.19877052191173306 1103 10-11 0.17285343820206833 1103 12-13 0.2024794051386749 1103 14-15 0.29917729782765434 1103 16-17 0.24341110265639543 1103 18-19 0.21946763153069782 1103 20-21 0.09760246287849128 1103 22-23 0.11271272742644811 1103 24-25 0.1789342903343325 1103 26-27 0.22125148031153685 1103 28-29 0.1319516799670737 1103 30-31 0.19913413446918327 1103 32-33 0.13337450239293247 1103 34-35 0.12822353240251516 1103 36-37 0.16983013798446933 1103 38-39 0.2240722149124892 1103 40-41 0.04881601604797936 1103 42-43 0.1139691410197301 1103 44-45 0.13960069237260342 1103 46-47 0.210748022688783 1103 48-49 -0.024238060385116 1103 50-51 -0.1725676877329576 1103 52-53 -0.007890232632796312 1103 54-55 -0.0059051402561749455 1103 56-57 -0.048092415346943085 1103 58-59 0.1169914120837845 1103 60-61 0.06491859633987929 1103 62-63 -0.1421287521042771 1103 64-65 0.01783046050133663 1103 66-67 0.05871417695009029 1103 68-69 0.04042019008859654 1103 70-71 0.14082809283272724 1103 72-73 0.08276230108785398 1103 74-75 0.08598923699209848 1103 76-77 0.023069311284004357 1103 78-79 0.07520387727343802 1103 80-81 0.13095420174524008 1103 82-83 0.2236270180200819 1103 84-85 0.025014244380699324 1103 86-87 -0.005425405689180707 1103 88-89 0.07958548229868967 1103 90-91 0.2627318168620292 1103 92-93 0.10483813623924476 1103 94-95 0.17380502849592716 1103 96-97 0.23393099845280574 1103 98-99 0.13003062542383503 1103 100-101 0.13609474049626158 1104 1 0.02577157985736278 1104 2 0.05721824555430999 1104 3 0.05164241212055032 1104 4 0.04043079372999614 1104 5 0.12738944797692398 1104 6 0.052103111908664346 1104 7 0.04699356649020103 1104 8 0.11907876687090635 1104 9 0.10966704979574615 1104 10-11 -0.020148292193901796 1104 12-13 -0.004445456737578013 1104 14-15 0.03190760836518791 1104 16-17 0.06301185997780578 1104 18-19 0.06908907016322274 1104 20-21 -0.005079948868335293 1104 22-23 0.04351029891206082 1104 24-25 0.07643117621759643 1104 26-27 0.005261409724894861 1104 28-29 -0.009214161897382667 1104 30-31 -0.11856735931350926 1104 32-33 0.05201907190975419 1104 34-35 0.06286035785105781 1104 36-37 0.10983595894908404 1104 38-39 -0.004384752662950575 1104 40-41 0.11864126790899832 1104 42-43 0.27071152658214714 1104 44-45 0.16257214580588908 1104 46-47 0.33302205304124044 1104 48-49 0.2522840821271899 1104 50-51 0.18158933527077892 1104 52-53 0.2220711463596814 1104 54-55 0.1858608768332246 1104 56-57 0.25236670578673426 1104 58-59 0.03249057469928829 1104 60-61 0.16582442187384316 1104 62-63 0.15504390803682355 1104 64-65 0.207274051778235 1104 66-67 0.1286601194596173 1104 68-69 0.07745483575476442 1104 70-71 0.04657962307094721 1104 72-73 0.1390657302916054 1104 74-75 0.11790868517260122 1104 76-77 0.07464842176909059 1104 78-79 0.13750409203325376 1104 80-81 0.15035162264072 1104 82-83 0.16545064875239035 1104 84-85 0.21178525796361214 1104 86-87 0.25332342465453905 1104 88-89 0.2974375662238913 1104 90-91 -0.010160666146916242 1104 92-93 0.028714123866215857 1104 94-95 0.1321692381062789 1104 96-97 0.12298698898032256 1104 98-99 0.1288023621573018 1104 100-101 0.12450198153783987 1105 1 0.09643941772630171 1105 2 0.08785940395942049 1105 3 -0.014485613758154159 1105 4 0.10382539067084195 1105 5 0.09314207172258193 1105 6 0.12014977664827597 1105 7 0.17838057119652717 1105 8 0.017403068288977863 1105 9 0.14491724460634714 1105 10-11 0.12231882854750609 1105 12-13 0.15602047970504174 1105 14-15 0.15034871195204147 1105 16-17 0.04736948079077763 1105 18-19 -0.0050848907923324305 1105 20-21 0.09673840813693602 1105 22-23 0.03477586154284751 1105 24-25 0.12487517039151896 1105 26-27 0.14017418228433343 1105 28-29 0.15297911079910165 1105 30-31 0.04066048910747355 1105 32-33 0.1860857163123555 1105 34-35 0.18636560160051374 1105 36-37 0.12500826693786138 1105 38-39 0.1364878678647088 1105 40-41 -0.03754538356571402 1105 42-43 0.08125178572468172 1105 44-45 0.15509216709707374 1105 46-47 0.22415636992255372 1105 48-49 0.23386196848127838 1105 50-51 0.32805323052862434 1105 52-53 0.32881940428985246 1105 54-55 0.10574390628072905 1105 56-57 0.12795873824362758 1105 58-59 0.13001374383043185 1105 60-61 0.2567460139979758 1105 62-63 0.3473025603871278 1105 64-65 0.3240041897499779 1105 66-67 0.14586940264079828 1105 68-69 0.09605001043883021 1105 70-71 0.1692251744200277 1105 72-73 0.3381350360923392 1105 74-75 0.26956066971496995 1105 76-77 0.21183718084660796 1105 78-79 0.24261060876358442 1105 80-81 0.218499317608007 1105 82-83 0.21573283179110092 1105 84-85 0.19574109652853622 1105 86-87 0.25200769303198456 1105 88-89 0.1863543631540665 1105 90-91 0.12407670063116427 1105 92-93 0.12485233695954179 1105 94-95 0.19200345498637006 1105 96-97 0.22099051942550574 1105 98-99 0.22943033985955452 1105 100-101 0.14082912115694057 1106 1 0.1454322517145954 1106 2 0.1475869199033042 1106 3 0.17858634561473252 1106 4 0.17677212851379664 1106 5 0.1422701980049439 1106 6 0.05307794987975001 1106 7 0.2055770102316643 1106 8 0.19165595915379185 1106 9 0.21772062637611356 1106 10-11 0.24550470224005494 1106 12-13 0.2524225110606082 1106 14-15 0.21982623282378455 1106 16-17 0.23610715888830214 1106 18-19 0.1637742278186849 1106 20-21 0.23859417042390163 1106 22-23 0.2710495175348697 1106 24-25 0.19515100539585006 1106 26-27 0.32083419364529675 1106 28-29 0.4058614638456888 1106 30-31 0.22071392508374998 1106 32-33 0.08586414158173028 1106 34-35 0.030223958632397796 1106 36-37 0.2217512169549778 1106 38-39 0.33683380398883145 1106 40-41 0.3460105100712454 1106 42-43 0.3997562772496437 1106 44-45 0.3773066241986598 1106 46-47 0.3576213220505835 1106 48-49 0.37394817923980384 1106 50-51 0.37179161644675673 1106 52-53 0.2538558256921988 1106 54-55 0.32546575965972124 1106 56-57 0.2875816938516351 1106 58-59 0.26736588548056517 1106 60-61 0.3126266593153417 1106 62-63 0.27426264109659826 1106 64-65 0.43042098678107266 1106 66-67 0.39295927435503586 1106 68-69 0.16538641517603025 1106 70-71 0.2527762297895251 1106 72-73 0.23657800994239153 1106 74-75 0.306899998916073 1106 76-77 0.19669519561586668 1106 78-79 0.37991257578810433 1106 80-81 0.40070443864533445 1106 82-83 0.525641334777383 1106 84-85 0.2758305507182115 1106 86-87 0.2538108436400819 1106 88-89 0.3818947349122013 1106 90-91 0.3996962228612162 1106 92-93 0.39778079690317014 1106 94-95 0.3432418722636399 1106 96-97 0.4030034537272087 1106 98-99 0.37988791452567483 1106 100-101 0.4611578187132537 1107 1 0.1596618588635863 1107 2 0.19906882679529048 1107 3 0.16450831434968194 1107 4 0.1360942137937613 1107 5 0.18757791389110423 1107 6 0.22303156765926246 1107 7 0.2819156779133323 1107 8 0.19965242911987247 1107 9 0.19205714504857951 1107 10-11 0.20177084928214128 1107 12-13 0.13361312278718884 1107 14-15 0.13450837819517147 1107 16-17 0.23285669826602629 1107 18-19 0.19865015453680002 1107 20-21 0.1870777379139028 1107 22-23 0.19768510553140572 1107 24-25 0.23563018729027618 1107 26-27 0.24723736528646612 1107 28-29 0.20572235086775947 1107 30-31 0.24590836325932486 1107 32-33 0.3045291959301011 1107 34-35 0.22997571101803516 1107 36-37 0.24052725853392332 1107 38-39 0.3544368166437337 1107 40-41 0.21577735267821652 1107 42-43 0.164273036489881 1107 44-45 0.27625350864057197 1107 46-47 0.3620111280671381 1107 48-49 0.22732966879284788 1107 50-51 0.34463090222119064 1107 52-53 0.21601827461954315 1107 54-55 0.3025205391841794 1107 56-57 0.31108690300951025 1107 58-59 0.26194536345322916 1107 60-61 0.48412045649333635 1107 62-63 0.36083099676772434 1107 64-65 0.24387570432782724 1107 66-67 0.37100182338983245 1107 68-69 0.35975806577004477 1107 70-71 0.3601626910315687 1107 72-73 0.32661155873803693 1107 74-75 0.320626037295078 1107 76-77 0.20909831336338414 1107 78-79 0.26507026523269417 1107 80-81 0.28875133285547605 1107 82-83 0.40012218737489746 1107 84-85 0.35588703799762555 1107 86-87 0.1857355878978808 1107 88-89 0.20850708127319706 1107 90-91 0.2596855788658132 1107 92-93 0.3397061811480384 1107 94-95 0.4066519591334625 1107 96-97 0.34979273967005753 1107 98-99 0.3537798106052463 1107 100-101 0.40479095791717157 1108 1 0.007559577827208841 1108 2 0.018017203995505326 1108 3 0.08535812563756195 1108 4 -0.014369865561789652 1108 5 -0.012091334278558463 1108 6 0.05865444211980986 1108 7 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-0.9523709993535334 2316 90-91 -0.9087691973367527 2316 92-93 -0.8553960514797367 2316 94-95 -1.0049889969512478 2316 96-97 -0.9060468735737501 2316 98-99 -0.9287283640653783 2316 100-101 -1.1217422500993486 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 21.0 14 94.0 15 297.0 16 728.0 17 1457.0 18 2310.0 19 3018.0 20 3802.0 21 5379.0 22 6429.0 23 5114.0 24 4088.0 25 4166.0 26 5076.0 27 6013.0 28 7372.0 29 9316.0 30 11502.0 31 14547.0 32 18358.0 33 23992.0 34 32686.0 35 49274.0 36 84767.0 37 200425.0 38 486332.0 39 197399.0 40 37.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.03262484219919 28.134595852479265 16.14207227319355 20.690707032127992 2 12.159526747563508 24.9528495908282 42.84196399085455 20.04565967075374 3 13.647811809539254 33.76517746741532 34.003861512577785 18.58314921046764 4 8.89913976024111 21.879603600437893 47.981680204349175 21.23957643497182 5 8.28302364864865 44.111739864864866 38.771030405405405 8.834206081081081 6 21.325253378378378 47.77609797297298 21.19831081081081 9.700337837837838 7 21.886402027027028 35.1866554054054 29.94940878378378 12.977533783783784 8 21.818496621621623 38.79560810810811 24.385304054054053 15.000591216216216 9 24.546030405405407 17.620861486486486 26.284543918918917 31.548564189189186 10-11 19.395734797297298 29.48268581081081 35.096199324324324 16.025380067567568 12-13 20.7660472972973 29.456798986486486 31.980658783783785 17.796494932432434 14-15 20.33585304054054 30.80684121621622 29.29991554054054 19.557390202702702 16-17 17.639442567567567 33.84598817567567 30.18091216216216 18.333657094594592 18-19 17.08631756756757 32.94484797297297 32.770650337837836 17.198184121621622 20-21 18.14413006756757 32.90950168918919 31.732094594594596 17.21427364864865 22-23 19.113277442655185 31.895778468856683 31.12283336345777 17.868110725030366 24-25 18.76059966216216 32.35152027027027 30.831461148648646 18.05641891891892 26-27 18.35899493243243 31.60506756756757 32.47377533783784 17.562162162162164 28-29 18.283899674290765 30.098346363533068 33.32734939591096 18.29040456626521 30-31 19.032831816505748 31.687047079450455 31.183826663079273 18.096294440964524 32-33 18.177660472972974 31.847339527027025 31.88327702702703 18.091722972972974 34-35 18.32014358108108 31.696663851351353 32.36735641891892 17.615836148648647 36-37 19.504307432432434 30.07753378378378 31.300464527027028 19.117694256756756 38-39 17.81570945945946 32.85291385135135 32.18876689189189 17.142609797297297 40-41 17.502449324324324 31.17778716216216 33.545101351351356 17.774662162162162 42-43 18.086444256756756 31.070945945945944 30.742271959459462 20.100337837837838 44-45 18.477364864864864 31.644467905405406 31.39995777027027 18.47820945945946 46-47 18.58445945945946 31.168454391891892 32.00924831081081 18.23783783783784 48-49 17.219936557127944 31.48490876654772 31.719915223627886 19.57523945269645 50-51 17.24013702639545 31.243150052856777 30.611994075310456 20.904718845437316 52-53 17.98897804054054 31.005278716216218 31.808699324324323 19.19704391891892 54-55 18.327523866393804 31.02179331360902 31.120274124631436 19.530408695365743 56-57 18.83424831081081 31.269552364864868 30.097381756756757 19.798817567567568 58-59 18.1640625 30.346410472972973 30.71570945945946 20.77381756756757 60-61 19.85091197328375 32.277785824897585 29.661650753780517 18.20965144803815 62-63 17.92179054054054 32.22639358108108 31.925422297297295 17.926393581081083 64-65 17.92297297297297 31.60751689189189 30.643538851351355 19.825971283783783 66-67 18.56376689189189 31.817694256756756 30.008614864864864 19.609923986486486 68-69 18.267947635135133 31.659881756756757 30.38543074324324 19.686739864864865 70-71 18.677533783783783 30.755447635135134 31.908065878378377 18.658952702702702 72-73 18.62369087837838 31.281967905405406 30.47622466216216 19.618116554054055 74-75 18.090329391891892 31.453927364864864 29.688640202702704 20.76710304054054 76-77 17.316427364864865 31.92010135135135 31.985135135135135 18.77833614864865 78-79 17.814967280294866 31.63781803316903 30.326496035459577 20.220718651076524 80-81 18.262128885990666 31.9883213331216 29.84205164764991 19.907498133237826 82-83 18.500523041929085 31.813069840139637 30.671415220118938 19.014991897812347 84-85 17.96583614864865 31.33619087837838 30.496114864864865 20.20185810810811 86-87 16.643369932432435 32.32487331081081 31.886064189189188 19.14569256756757 88-89 18.42930743243243 30.129645270270274 30.947466216216217 20.493581081081082 90-91 17.72014358108108 30.686824324324323 32.187626689189194 19.405405405405403 92-93 19.15434966216216 31.747846283783783 30.395777027027027 18.702027027027025 94-95 18.346157094594595 29.567018581081083 31.141173986486486 20.945650337837836 96-97 18.810388513513512 29.827618243243244 31.239484797297294 20.122508445945947 98-99 17.59987331081081 30.834712837837834 30.894510135135135 20.670903716216216 100-101 17.239949324324325 30.547972972972975 31.877744932432435 20.33433277027027 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 149.0 1 98.5 2 141.5 3 214.5 4 140.0 5 106.5 6 217.5 7 458.5 8 695.0 9 845.0 10 726.5 11 542.5 12 549.0 13 580.0 14 601.0 15 608.0 16 626.0 17 659.5 18 678.5 19 709.0 20 818.0 21 969.0 22 1140.0 23 1366.5 24 1613.0 25 2049.0 26 3056.0 27 4307.5 28 5088.5 29 7475.5 30 14243.5 31 21600.0 32 39108.5 33 61973.5 34 82261.0 35 102600.0 36 125314.0 37 134922.5 38 115693.0 39 95371.5 40 73891.0 41 53384.5 42 41566.0 43 34101.0 44 31232.5 45 27099.5 46 20532.0 47 16161.5 48 17768.0 49 14957.5 50 9567.0 51 5688.0 52 2288.5 53 1276.5 54 872.0 55 668.5 56 516.0 57 418.5 58 341.0 59 266.5 60 225.0 61 204.0 62 173.0 63 137.5 64 101.5 65 66.5 66 37.5 67 21.0 68 21.5 69 21.5 70 21.0 71 16.0 72 9.0 73 5.0 74 6.0 75 20.5 76 22.5 77 9.5 78 10.0 79 14.0 80 10.5 81 3.5 82 2.0 83 1.0 84 1.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046114864864864866 2 0.0019425675675675677 3 6.756756756756757E-4 4 0.012162162162162161 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0018581081081081082 24-25 0.0 26-27 0.0 28-29 0.02326858108108108 30-31 0.01896114864864865 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.03509290540540541 50-51 0.011866554054054054 52-53 0.0 54-55 0.001097972972972973 56-57 0.0 58-59 0.0 60-61 1.266891891891892E-4 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.3783783783783786E-4 80-81 0.011148648648648648 82-83 0.005109797297297297 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1184000.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 10.656774546432073 #Duplication Level Percentage of deduplicated Percentage of total 1 72.54205019946409 7.730642741116467 2 9.733006699792039 2.074449161171933 3 3.764453400801293 1.2035079354866665 4 2.020475966055318 0.8612702738694429 5 1.3560277216463892 0.722544085414876 6 0.9706946439818296 0.6206684384606103 7 0.7062772838902112 0.5268646447179067 8 0.5737590086501799 0.48915363193354705 9 0.47719229373852157 0.4576797620699593 >10 5.028838103953167 11.980547394628584 >50 1.2105277551698934 9.261784451329582 >100 1.3294259987561257 30.30742145658011 >500 0.18273622671977957 13.31624405734991 >1k 0.10214077301367593 18.7205177773507 >5k 0.002393924367508029 1.72670418851971 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 8343 0.7046452702702702 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 6195 0.5232263513513514 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 5767 0.48707770270270273 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 4331 0.36579391891891894 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 4086 0.34510135135135134 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 3856 0.3256756756756757 No Hit ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC 3673 0.3102195945945946 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 3437 0.29028716216216216 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 3433 0.2899493243243243 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3223 0.27221283783783784 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3160 0.2668918918918919 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3095 0.26140202702702703 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 3046 0.2572635135135135 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 2894 0.2444256756756757 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 2854 0.2410472972972973 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 2846 0.24037162162162162 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 2567 0.21680743243243242 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 2529 0.21359797297297298 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 2481 0.2095439189189189 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 2479 0.209375 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 2457 0.20751689189189187 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 2442 0.20625000000000002 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 2404 0.20304054054054052 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 2376 0.20067567567567568 No Hit ATATAGGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATT 2376 0.20067567567567568 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 2299 0.1941722972972973 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 2296 0.19391891891891894 No Hit CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC 2278 0.19239864864864864 No Hit TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC 2268 0.19155405405405407 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 2252 0.1902027027027027 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 2168 0.18310810810810813 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 2136 0.1804054054054054 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 2125 0.17947635135135137 No Hit CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC 2077 0.1754222972972973 No Hit ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG 2051 0.17322635135135137 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 2041 0.17238175675675674 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 2008 0.16959459459459458 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 1990 0.16807432432432431 No Hit ATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTC 1956 0.1652027027027027 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 1950 0.16469594594594594 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 1938 0.16368243243243244 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 1936 0.1635135135135135 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 1918 0.16199324324324324 No Hit GTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTC 1905 0.16089527027027029 No Hit CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG 1901 0.16055743243243242 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 1859 0.15701013513513515 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1840 0.1554054054054054 No Hit TTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGT 1836 0.15506756756756757 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 1816 0.15337837837837837 No Hit CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT 1779 0.15025337837837838 No Hit GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG 1738 0.14679054054054055 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1718 0.14510135135135135 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 1710 0.14442567567567569 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 1627 0.13741554054054053 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 1626 0.13733108108108108 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 1619 0.13673986486486486 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1571 0.13268581081081082 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1569 0.1325168918918919 No Hit CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA 1567 0.132347972972973 No Hit ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG 1558 0.13158783783783784 No Hit AAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCA 1547 0.1306587837837838 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1523 0.12863175675675675 No Hit GGCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTG 1519 0.12829391891891892 No Hit CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC 1504 0.12702702702702703 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1500 0.1266891891891892 No Hit GCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGG 1489 0.12576013513513512 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1488 0.12567567567567567 No Hit ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA 1446 0.12212837837837838 No Hit ACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGGTAAAA 1442 0.12179054054054055 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 1438 0.12145270270270271 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 1434 0.12111486486486486 No Hit CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA 1423 0.12018581081081081 No Hit GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA 1421 0.1200168918918919 No Hit CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC 1403 0.11849662162162161 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 1399 0.11815878378378378 No Hit TCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAA 1397 0.11798986486486486 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1380 0.11655405405405406 No Hit CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT 1370 0.11570945945945947 No Hit GATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAG 1365 0.11528716216216216 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 1349 0.1139358108108108 No Hit AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT 1342 0.1133445945945946 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 1323 0.11173986486486485 No Hit TCCTTTTGGTGTGTGGATTAGCATTATTTGTTTGATAATAAGTTTAACTA 1315 0.11106418918918919 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 1313 0.11089527027027027 No Hit GTTTATATTCTTATCCTCCCAGGATTTGGAATTATTTCACATGTAGTTAC 1309 0.11055743243243243 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 1306 0.11030405405405405 No Hit ATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATG 1301 0.10988175675675675 No Hit TAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCA 1290 0.1089527027027027 No Hit CATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCC 1276 0.10777027027027027 No Hit TTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAAC 1272 0.10743243243243243 No Hit ACTTACTACTCCGGAAAAAAAGAACCTTTCGGCTATATAGGAATAGTATG 1260 0.10641891891891891 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 1259 0.10633445945945946 No Hit TAGTATAGCTGGAGATCATTTAATATTACCTCCGTGTAGGGTTGCAAGTC 1241 0.1048141891891892 No Hit CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC 1231 0.1039695945945946 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 1223 0.10329391891891891 No Hit TTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTAT 1222 0.10320945945945946 No Hit GAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAA 1217 0.10278716216216216 No Hit TTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGT 1213 0.10244932432432433 No Hit ATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC 1212 0.10236486486486487 No Hit ATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTAT 1199 0.1012668918918919 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.445945945945946E-5 0.0 10-11 0.0 0.0 0.0 5.067567567567568E-4 0.0 12-13 0.0 0.0 0.0 0.0013935810810810812 0.0 14-15 0.0 0.0 0.0 0.002533783783783784 0.0 16-17 0.0 0.0 0.0 0.006081081081081081 0.0 18-19 0.0 0.0 0.0 0.010008445945945946 0.0 20-21 0.0 0.0 0.0 0.016849662162162164 0.0 22-23 0.0 0.0 0.0 0.03289695945945946 0.0 24-25 0.0 0.0 0.0 0.05249155405405405 0.0 26-27 0.0 0.0 0.0 0.06059966216216216 0.0 28-29 0.0 0.0 0.0 0.0680320945945946 0.0 30-31 0.0 0.0 0.0 0.08091216216216215 0.0 32-33 0.0 0.0 0.0 0.0983108108108108 0.0 34-35 0.0 0.0 0.0 0.12335304054054054 0.0 36-37 0.0 0.0 0.0 0.15485641891891894 0.0 38-39 0.0 0.0 0.0 0.20114020270270272 0.0 40-41 0.0 0.0 0.0 0.26313344594594595 0.0 42-43 0.0 0.0 0.0 0.31929898648648647 0.0 44-45 0.0 0.0 0.0 0.38048986486486486 0.0 46-47 0.0 0.0 0.0 0.4581503378378379 0.0 48-49 0.0 0.0 0.0 0.5390202702702702 0.0 50-51 0.0 0.0 0.0 0.6285472972972973 0.0 52-53 0.0 0.0 0.0 0.7164695945945947 0.0 54-55 0.0 0.0 0.0 0.8123733108108109 0.0 56-57 0.0 0.0 0.0 0.9216216216216215 0.0 58-59 0.0 0.0 0.0 1.0451013513513514 0.0 60-61 0.0 0.0 0.0 1.1748310810810811 0.0 62-63 0.0 0.0 0.0 1.3077280405405405 0.0 64-65 0.0 0.0 0.0 1.4510135135135136 0.0 66-67 0.0 0.0 0.0 1.613302364864865 0.0 68-69 0.0 0.0 0.0 1.7983530405405406 0.0 70-71 0.0 0.0 0.0 2.0215371621621623 0.0 72-73 0.0 0.0 0.0 2.2516469594594595 0.0 74-75 0.0 0.0 0.0 2.4795608108108107 0.0 76-77 0.0 0.0 0.0 2.7338682432432435 0.0 78-79 0.0 0.0 0.0 3.0274915540540537 0.0 80-81 0.0 0.0 0.0 3.3679054054054056 0.0 82-83 0.0 0.0 0.0 3.7228462837837837 0.0 84-85 0.0 0.0 0.0 4.069087837837838 0.0 86-87 0.0 0.0 0.0 4.439442567567568 0.0 88-89 0.0 0.0 0.0 4.855320945945946 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGGCGA 35 2.7897288E-4 54.278812 6 GACATAT 335 0.0 52.489265 1 TAACTTT 1205 0.0 46.90275 6 ACATATA 390 0.0 45.06218 2 TCAGGCC 55 4.964652E-5 43.179977 3 GGGATGC 220 0.0 43.176327 7 ATGGGCG 90 1.1979864E-8 42.216854 5 ACCTGGG 105 1.027729E-9 40.712547 3 GGTAACA 70 4.3140735E-6 40.70911 8 ATGGGTA 330 0.0 40.297905 5 ATGTATT 60 8.2661965E-5 39.603386 1 TACTAAG 60 8.2661965E-5 39.603386 1 TACCTGG 120 8.731149E-11 39.581642 2 CATGGGT 460 0.0 38.20489 4 AACTTTA 1565 0.0 36.72057 7 GTATGGT 65 1.3285427E-4 36.536903 4 GGGAGGT 130 2.055458E-10 36.533817 7 ACTTTAT 1555 0.0 36.34586 8 CTATAAC 1805 0.0 36.05109 3 CATTCCG 265 0.0 35.844498 9 >>END_MODULE