FastQCFastQC Report
Fri 10 Feb 2017
SRR2031627_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031627_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176466
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT139487.904072172543152No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT85424.8405925220722406No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT58573.3190529620436795No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC48772.763705189668265No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT36012.040619722779459No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT35682.0219192365668173No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC34971.9816848571396188No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA30291.716477961760339No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC28771.6303423889021114No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC27801.575374293064953No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG24251.3742023959289609No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC23181.3135674860879716No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA20721.1741638615937349No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC19511.1055954121473826No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16820.9531581154443349No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG16520.9361576734328426No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC15790.8947899312048779No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA15600.8840229845975995No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14170.8029875443428196No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13980.7922205977355412No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT13930.7893871907336256No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12560.7117518388811442No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG11760.6664173268504981No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC11500.6516836104405381No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10470.5933154262010811No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG9820.5564811351761813No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT9700.5496809583715843No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8420.4771457391225505No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7950.450511713304546No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6730.3813765824578106No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT6600.3740097242528306No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5890.33377534482563215No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG5200.2946743281991998No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA5190.2941076467988168No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA4740.2686069837815783No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT4740.2686069837815783No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG4350.24650640916663832No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG3870.21930570194825066No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA3550.2011718971359922No Hit
GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3490.19777180873369374No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA3170.17963800392143528No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA3070.17397118991760452No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3020.17113778291568915No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT3000.17000442011492298No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2990.1694377387145399No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA2860.16207088050955992No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT2770.1569707479061122No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2740.15527070370496301No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2600.1473371640995999No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG2390.1354368546915553No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC2330.13203676628925687No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2320.13147008488887377No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT2300.13033672208810762No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT2260.12806999648657533No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2200.12466990808427686No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC2130.12070313828159533No Hit
GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC2080.11786973127967994No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2010.1139029614769984No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1960.11106955447508303No Hit
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC1870.10596942187163533No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1840.1042693776704861No Hit
TATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1840.1042693776704861No Hit
GTACATGGGCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAA1820.10313601486971996No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1810.10256933346933687No Hit
GTACATGGGCTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAA1770.10030260786780457No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCATG255.2483716E-576.042493
GTACCCA301.2693118E-463.621711
TACCCAT301.2819037E-463.4949762
CGTAATG250.00496388457.1454732
AAAGTAC504.204976E-757.0318643
GCGCTTG250.005003154357.0318649
AAGGCGC250.005003154357.0318646
TATATGG451.4880492E-552.9124762
AGTACTC601.4793277E-647.5265585
AAGTACT652.566945E-643.8706674
ATGGGGA554.9298433E-543.205965
ATGGGAG554.9298433E-543.205965
AAAAAGT704.157453E-640.899671
TAATGAT608.234991E-539.6054654
AATGTAT500.001607680638.0212446
GAAGGCA500.001607680638.0212449
CGCAGAA3800.037.5209669
GTATATG651.2886898E-436.7048341
TTATGTA1106.921073E-834.5647663
TATAGGC550.002566676834.5647663
Produced by FastQC (version 0.11.5)