##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031627_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 176466 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10660977185407 33.0 31.0 34.0 31.0 34.0 2 32.22126075277957 34.0 31.0 34.0 31.0 34.0 3 32.3581369782281 34.0 31.0 34.0 31.0 34.0 4 35.29500867022543 37.0 35.0 37.0 33.0 37.0 5 35.23241304273911 37.0 35.0 37.0 33.0 37.0 6 35.63184409461313 37.0 36.0 37.0 35.0 37.0 7 35.778665578638375 37.0 36.0 37.0 35.0 37.0 8 35.83175229222626 37.0 37.0 37.0 35.0 37.0 9 37.67654959028935 39.0 38.0 39.0 35.0 39.0 10-11 37.652712137182235 39.0 38.0 39.0 35.0 39.0 12-13 37.71973354640554 39.0 38.5 39.0 35.0 39.0 14-15 38.79589552661703 40.5 38.5 41.0 36.0 41.0 16-17 38.956940713791894 40.5 38.5 41.0 36.0 41.0 18-19 39.03300919157232 41.0 39.0 41.0 36.0 41.0 20-21 39.0260390103476 41.0 39.0 41.0 36.0 41.0 22-23 38.889893803905565 41.0 39.0 41.0 35.0 41.0 24-25 38.65634173155169 40.5 38.5 41.0 35.0 41.0 26-27 38.32998424625707 40.0 37.5 41.0 34.5 41.0 28-29 38.25874106060091 40.0 38.0 41.0 35.0 41.0 30-31 38.07833236997495 40.0 37.5 41.0 35.0 41.0 32-33 37.64250053834733 40.0 35.5 41.0 34.0 41.0 34-35 37.20300227805923 40.0 35.0 41.0 33.0 41.0 36-37 36.858063309646056 39.5 35.0 41.0 32.0 41.0 38-39 36.55647546836218 39.0 35.0 41.0 31.5 41.0 40-41 36.52725737535843 39.5 35.0 41.0 31.0 41.0 42-43 36.10977752088221 39.0 35.0 41.0 30.0 41.0 44-45 35.54048655265037 39.0 35.0 41.0 25.5 41.0 46-47 34.93065236362812 38.5 35.0 40.5 21.5 41.0 48-49 34.57750501513039 38.5 35.0 40.5 15.0 41.0 50-51 33.9504465449435 38.0 34.0 40.5 12.0 41.0 52-53 34.239111216891644 38.5 35.0 40.0 10.5 41.0 54-55 34.43707853070846 39.0 35.0 41.0 10.0 41.0 56-57 34.020598868903924 39.0 34.5 41.0 10.0 41.0 58-59 33.7050253306586 39.0 34.0 41.0 9.0 41.0 60-61 32.892948216653636 38.0 32.5 40.0 8.0 41.0 62-63 32.79288644838099 37.0 32.5 40.0 8.0 41.0 64-65 32.49621173483844 37.0 32.5 39.5 7.5 41.0 66-67 31.93064669681412 36.5 31.5 39.0 7.0 40.5 68-69 31.380744732696385 36.0 31.0 39.0 7.0 39.0 70-71 30.62482574546938 35.0 30.0 37.5 7.0 39.0 72-73 30.02689753266918 35.0 29.5 37.0 7.0 38.0 74-75 29.779076422653656 35.0 29.0 37.0 7.0 37.0 76-77 29.449055342105563 35.0 29.0 36.0 7.0 36.5 78-79 29.04414448108984 35.0 28.5 35.5 7.0 36.0 80-81 28.61803406888579 34.0 26.0 35.0 7.0 36.0 82-83 28.200701551573673 34.0 25.5 35.0 7.0 35.5 84-85 28.011512132648782 34.0 25.0 35.0 7.0 35.0 86-87 28.22897045323178 34.0 26.0 35.0 7.0 35.5 88-89 27.937461040653723 34.0 25.0 35.0 7.0 35.0 90-91 27.611236725488197 34.0 24.0 35.0 7.0 35.0 92-93 27.380305554611084 34.0 23.5 35.0 7.0 35.0 94-95 27.141749685491824 34.0 21.5 35.0 7.0 35.0 96-97 26.910169664411274 34.0 19.5 35.0 7.0 35.0 98-99 27.15569571475525 34.0 20.0 35.0 7.0 35.0 100-101 26.57235104779391 33.0 19.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.18692897436853073 1101 2 0.08939948159934374 1101 3 0.21540269652269473 1101 4 -0.2976879254740723 1101 5 -0.12955011198609867 1101 6 -0.27056725410796645 1101 7 -0.2212713640588646 1101 8 -0.06723695171650945 1101 9 0.03632118522749295 1101 10-11 0.06868438730373327 1101 12-13 0.24245219444814836 1101 14-15 -0.19791901735869288 1101 16-17 0.17884323801315105 1101 18-19 0.4401885808910677 1101 20-21 0.07933816246019632 1101 22-23 0.1765337567820282 1101 24-25 -0.05740219200072261 1101 26-27 -0.6342764918470749 1101 28-29 -0.10367561763964517 1101 30-31 -0.14379436797009504 1101 32-33 -0.5064650473864347 1101 34-35 -0.1398595293344158 1101 36-37 -0.035818087432012646 1101 38-39 -0.05134097698762474 1101 40-41 0.10842684179709039 1101 42-43 -0.2926512111839301 1101 44-45 -0.322175419609934 1101 46-47 -0.372895523754579 1101 48-49 0.08428853599981068 1101 50-51 -1.0856506862256055 1101 52-53 -1.0563369905896138 1101 54-55 -0.25315409809355316 1101 56-57 -0.6263328295056283 1101 58-59 -0.5289658678687559 1101 60-61 -0.4641370575264361 1101 62-63 -0.47169415401157266 1101 64-65 -0.03261791314811546 1101 66-67 -0.3272174071509042 1101 68-69 -0.0651640241896203 1101 70-71 -0.2257802128631532 1101 72-73 -0.25983320849028857 1101 74-75 -0.4766441751553998 1101 76-77 0.06349397162925996 1101 78-79 0.27601425639081256 1101 80-81 -0.020949167444122452 1101 82-83 -0.16536404719374787 1101 84-85 0.40819648966028055 1101 86-87 0.5831334093299532 1101 88-89 0.9094744028482857 1101 90-91 0.7739184125685554 1101 92-93 0.4072748404956137 1101 94-95 0.51206798502567 1101 96-97 0.6046873146881566 1101 98-99 0.6828305847778573 1101 100-101 0.7034195706394506 1102 1 -0.5801751617323632 1102 2 -0.02650465783420941 1102 3 0.23479267473620524 1102 4 -0.31217594290326645 1102 5 -0.95090087451333 1102 6 -0.35673283145001733 1102 7 -0.12840644031159343 1102 8 -0.27611494735921127 1102 9 0.1233582222645282 1102 10-11 0.337366304515065 1102 12-13 0.1460197325745085 1102 14-15 0.05137292163912832 1102 16-17 -0.015819071355039682 1102 18-19 0.47009054167538267 1102 20-21 0.5581235655103072 1102 22-23 0.055074061792915074 1102 24-25 0.3214104441648544 1102 26-27 0.3823901748195908 1102 28-29 0.1351043387873716 1102 30-31 0.15242022897979268 1102 32-33 0.0024401813717318532 1102 34-35 -0.13158066223202525 1102 36-37 -0.47745752098320793 1102 38-39 -0.620976053240355 1102 40-41 -0.7529021342377646 1102 42-43 -0.9266109061730461 1102 44-45 -1.7054815198277993 1102 46-47 -2.6017898592665603 1102 48-49 -1.4472746448280773 1102 50-51 -1.4709992701035972 1102 52-53 -1.3984121539883105 1102 54-55 -1.1768741416665378 1102 56-57 -0.7040289079370012 1102 58-59 -0.6488460421606916 1102 60-61 -1.7304769268074836 1102 62-63 -2.2611277052098337 1102 64-65 -1.4729501571568306 1102 66-67 -1.5624025923360882 1102 68-69 -1.0876585775665255 1102 70-71 -1.1404043958696874 1102 72-73 -1.071843012411854 1102 74-75 -1.2661321925845925 1102 76-77 -1.1502587516822835 1102 78-79 -1.1874661357660479 1102 80-81 -1.130616923435408 1102 82-83 -0.8713825657122669 1102 84-85 -1.0765529656774078 1102 86-87 -1.087536525310572 1102 88-89 -1.490580063382648 1102 90-91 -1.2816643761024693 1102 92-93 -0.604544331617678 1102 94-95 -0.3004864812052652 1102 96-97 -1.1167723803009508 1102 98-99 -1.587921049209072 1102 100-101 -1.4640913226067376 1103 1 0.46748279033734974 1103 2 0.3835171286581698 1103 3 0.3404026965226947 1103 4 0.38613560393768864 1103 5 0.3042734174256694 1103 6 0.1485503929508596 1103 7 0.19784628299995433 1103 8 0.32982187181289646 1103 9 0.06573294993337697 1103 10-11 0.24147850495078416 1103 12-13 0.3784816062128584 1103 14-15 0.42340451205307517 1103 16-17 0.4729608850719771 1103 18-19 0.1056297573616618 1103 20-21 -0.038308896363339784 1103 22-23 -0.03302506674739192 1103 24-25 -0.039019839059548644 1103 26-27 0.3252823316823381 1103 28-29 0.15367732353682584 1103 30-31 0.378264455559318 1103 32-33 0.31706436437827534 1103 34-35 0.44837576478322205 1103 36-37 0.33182897139152345 1103 38-39 0.6471884347770782 1103 40-41 0.48710331238532945 1103 42-43 0.2514664358748888 1103 44-45 0.37635399215477605 1103 46-47 0.5976927115395441 1103 48-49 0.3306120654115787 1103 50-51 0.5724375490685176 1103 52-53 0.8627806564692051 1103 54-55 0.6843459019064468 1103 56-57 0.9214612881414297 1103 58-59 0.7283870733077151 1103 60-61 0.8446864718853249 1103 62-63 1.0650705518707824 1103 64-65 1.3313526750871816 1103 66-67 1.5771943575549798 1103 68-69 1.3686595052221477 1103 70-71 1.1823080224309699 1103 72-73 1.019578556215592 1103 74-75 0.4682087660210712 1103 76-77 0.20319985398220197 1103 78-79 0.19145543286139954 1103 80-81 0.47169789137940654 1103 82-83 0.4375771292768391 1103 84-85 0.45966707789557404 1103 86-87 0.6419569387417177 1103 88-89 0.8727096969659307 1103 90-91 0.9099478243332619 1103 92-93 0.12786307578972966 1103 94-95 0.49000916149625695 1103 96-97 1.2223343735116856 1103 98-99 0.4953305847778573 1103 100-101 0.4901842765218021 1104 1 -0.3973047913619929 1104 2 -0.6177083615379111 1104 3 -0.27724436230083427 1104 4 -0.057083350310676906 1104 5 -0.41386383747629196 1104 6 -0.2076587573759383 1104 7 -0.19553606994121964 1104 8 -0.08398531772958506 1104 9 -0.4289565925502856 1104 10-11 -0.22767999178123688 1104 12-13 -0.24958211927734908 1104 14-15 -0.4530252265090198 1104 16-17 -0.3820227750587435 1104 18-19 -0.29796501388016594 1104 20-21 -0.14349680486007088 1104 22-23 -0.20071297524412302 1104 24-25 -0.18669140768700032 1104 26-27 -0.4988598251804035 1104 28-29 -0.32855306862003175 1104 30-31 -0.21609828953872068 1104 32-33 -0.3301987075171553 1104 34-35 -0.5109788103801733 1104 36-37 -0.487411224686916 1104 38-39 -0.3068556828699798 1104 40-41 -0.12605028238591132 1104 42-43 -0.12128683209896707 1104 44-45 -0.14876855686483026 1104 46-47 -0.4756324518591484 1104 48-49 -0.8595431633465935 1104 50-51 -0.6469251960295281 1104 52-53 -0.8449399317660848 1104 54-55 -0.9085871046294969 1104 56-57 -1.158195574603667 1104 58-59 -1.2930590051236521 1104 60-61 -1.5811713712519264 1104 62-63 -0.8183036311357554 1104 64-65 -0.5808205275272016 1104 66-67 -0.9174951849286828 1104 68-69 -0.795760429418376 1104 70-71 -0.6820710625363553 1104 72-73 -0.9683707901896348 1104 74-75 -1.2082618222142223 1104 76-77 -0.9340550479785819 1104 78-79 -1.088160580210495 1104 80-81 -0.6741354419539292 1104 82-83 -1.0993918249715264 1104 84-85 -0.9422937064181518 1104 86-87 -0.7236476364216813 1104 88-89 -0.5553948781974647 1104 90-91 -0.3647662279543198 1104 92-93 -0.3825535908769382 1104 94-95 -0.4146068515756376 1104 96-97 -0.7343649728935411 1104 98-99 -0.3247266047646278 1104 100-101 -0.4187209522363702 1105 1 -0.15950449106169273 1105 2 -0.027055208384759055 1105 3 -0.050893010949479844 1105 4 0.17170794098061748 1105 5 0.0028028291903723357 1105 6 -0.19902512374230952 1105 7 -0.07560363750878452 1105 8 0.2123735411292742 1105 9 -0.13259773369142636 1105 10-11 0.012841779990537816 1105 12-13 -0.02632761477283907 1105 14-15 -0.3253037550375453 1105 16-17 -0.16674499728096492 1105 18-19 -0.07968423309939254 1105 20-21 -0.30669124930451375 1105 22-23 -0.2777587710399203 1105 24-25 0.20829733105173887 1105 26-27 0.1968045892340058 1105 28-29 -0.10248324880021897 1105 30-31 0.07669450325406757 1105 32-33 0.16295069188224431 1105 34-35 0.38438530373394286 1105 36-37 0.5888838203581344 1105 38-39 0.2852051279408272 1105 40-41 0.22567644434081302 1105 42-43 0.10271842315628277 1105 44-45 0.302526488180213 1105 46-47 1.1055424730657748 1105 48-49 0.5649125423591173 1105 50-51 0.020867596763267215 1105 52-53 0.6204316898555362 1105 54-55 0.5335112437188485 1105 56-57 0.6734485695404686 1105 58-59 0.7190468507322052 1105 60-61 0.6640425927120361 1105 62-63 0.7538622847801619 1105 64-65 0.4798025955959204 1105 66-67 0.6288636739301765 1105 68-69 0.4006547357467909 1105 70-71 0.393754763289472 1105 72-73 0.32432815875931453 1105 74-75 0.42518412373172154 1105 76-77 -0.10015865158218418 1105 78-79 0.06884017054025549 1105 80-81 0.13217086435237846 1105 82-83 0.1707846014548977 1105 84-85 -0.0661675802920243 1105 86-87 0.2722232344491893 1105 88-89 -0.0797942775968643 1105 90-91 -0.17848619792428977 1105 92-93 0.2325928055194595 1105 94-95 0.4197587640399796 1105 96-97 -0.4137944023229707 1105 98-99 -0.2724555837436071 1105 100-101 -0.2569341654495787 1106 1 -0.2986936802508815 1106 2 -0.15104169487124608 1106 3 -0.05849436230083427 1106 4 0.22069442746710166 1106 5 -0.2846971708096291 1106 6 -0.15071431293149828 1106 7 -0.3455360699412182 1106 8 -0.19995753995180365 1106 9 -0.2011788147725042 1106 10-11 -0.3096244362256826 1106 12-13 -0.20791545261067768 1106 14-15 -0.3835807820645769 1106 16-17 -0.013619997280962082 1106 18-19 -0.09345112499128305 1106 20-21 -0.21919124930451517 1106 22-23 -0.3014074196885659 1106 24-25 -0.2064830743536632 1106 26-27 -0.2124014918470749 1106 28-29 -0.08896973528670316 1106 30-31 -0.3108899562053864 1106 32-33 -0.2899209297393739 1106 34-35 -0.05125658815795475 1106 36-37 -0.2019945580202389 1106 38-39 -0.30998068286997693 1106 40-41 -0.290286393497027 1106 42-43 -0.3705923876545256 1106 44-45 0.43977310980183404 1106 46-47 0.8059647703630759 1106 48-49 0.24219294776452216 1106 50-51 -0.07921686269619244 1106 52-53 -0.019939931766081997 1106 54-55 -0.22282321574061115 1106 56-57 0.0688877587296588 1106 58-59 -0.5857673384569893 1106 60-61 -0.2832547045852607 1106 62-63 0.22996025775312745 1106 64-65 0.30286002802835554 1106 66-67 0.03840759284909723 1106 68-69 -0.009649318307264565 1106 70-71 -0.16401550698079959 1106 72-73 0.1364903209214745 1106 74-75 0.07923817778577913 1106 76-77 -0.1455133813119147 1106 78-79 -0.37913280243271785 1106 80-81 -0.23455210862059062 1106 82-83 -0.062239047193749286 1106 84-85 -0.42875203975148324 1106 86-87 -0.4920504141994613 1106 88-89 -0.3831726559752404 1106 90-91 -0.03803011684320978 1106 92-93 -0.10651192421027034 1106 94-95 -0.8493290737978576 1106 96-97 0.18612113821756893 1106 98-99 0.27492617301314937 1106 100-101 0.1986401588747455 1107 1 0.2472182694346543 1107 2 0.29620673280171417 1107 3 0.27700092071803084 1107 4 0.30599316960546474 1107 5 0.08613616252370804 1107 6 0.2926032971314001 1107 7 0.10359915018456434 1107 8 0.3451289380356144 1107 9 0.28003187705139254 1107 10-11 0.16769474616425128 1107 12-13 0.20423077380441157 1107 14-15 0.08792079026246569 1107 16-17 -0.09834877086587568 1107 18-19 -0.014815118701967833 1107 20-21 -0.160464834210174 1107 22-23 -0.14967785994013383 1107 24-25 0.25257746023753214 1107 26-27 0.2886794829956898 1107 28-29 0.3308415854680149 1107 30-31 0.02761240228517181 1107 32-33 0.49258693189584335 1107 34-35 0.4500405816533686 1107 36-37 0.7741846872627747 1107 38-39 1.1481364554948001 1107 40-41 0.8155115624778162 1107 42-43 0.8799579268108815 1107 44-45 0.6191166632609537 1107 46-47 1.1976510911177911 1107 48-49 0.6251921616009994 1107 50-51 0.16282323164342927 1107 52-53 0.2810427726364324 1107 54-55 0.3987373188505856 1107 56-57 0.42867549457871945 1107 58-59 0.025926843932950305 1107 60-61 0.68042454069775 1107 62-63 1.0924012954889761 1107 64-65 1.2550023236258383 1107 66-67 0.4762417123459528 1107 68-69 0.8992736376675765 1107 70-71 0.7107486439625994 1107 72-73 0.6382198806699009 1107 74-75 0.6985778004272873 1107 76-77 0.9075330023358852 1107 78-79 1.053414367378604 1107 80-81 1.0483488347756307 1107 82-83 1.0936768333093951 1107 84-85 0.7204421426384542 1107 86-87 0.8877255291967643 1107 88-89 1.1967015578612354 1107 90-91 1.0543244429052194 1107 92-93 1.1663850883683473 1107 94-95 0.9805058318625193 1107 96-97 0.7910543143181954 1107 98-99 0.7028546321326488 1107 100-101 1.436887014220659 1108 1 0.07100328944608947 1108 2 0.2334280020984565 1108 3 0.2583616983052295 1108 4 0.05819442746710024 1108 5 0.21340888979643324 1108 6 -0.20185067656785805 1108 7 -0.1337936456987947 1108 8 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-0.8014273953483197 2316 62-63 -1.0889277944557065 2316 64-65 -1.2303352530961433 2316 66-67 -1.3543258408858456 2316 68-69 -1.4199655833675067 2316 70-71 -1.0426801656153337 2316 72-73 -0.6284695184359563 2316 74-75 -0.8475690511298843 2316 76-77 -1.262619305006691 2316 78-79 -0.9533295895813119 2316 80-81 -1.328251907817382 2316 82-83 -1.2464383443825042 2316 84-85 -1.3795427024020874 2316 86-87 -1.4527306350829932 2316 88-89 -1.229759001356772 2316 90-91 -1.411160136923531 2316 92-93 -1.782277486459268 2316 94-95 -1.570187507532797 2316 96-97 -1.3496594842723901 2316 98-99 -1.1130883852197826 2316 100-101 -1.7087142587959363 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 37.0 8 187.0 9 207.0 10 183.0 11 219.0 12 278.0 13 372.0 14 565.0 15 859.0 16 1331.0 17 2128.0 18 2259.0 19 2879.0 20 3780.0 21 3978.0 22 2835.0 23 2393.0 24 2364.0 25 2070.0 26 1974.0 27 2067.0 28 2336.0 29 2464.0 30 2752.0 31 3486.0 32 4382.0 33 5899.0 34 8822.0 35 16740.0 36 25597.0 37 35438.0 38 31774.0 39 3803.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.16632992800432 17.2533507868302 13.626817677356934 13.953501607808544 2 14.238529189382785 30.10636544004366 30.158666992603877 25.496438377969678 3 32.68643500175962 25.98907897873693 29.851169866155054 11.473316153348394 4 9.674085850556438 16.371224165341815 44.82171246877129 29.132977515330456 5 7.832402932607943 46.010256177273995 33.52793189807427 12.629408992043798 6 22.51428960647531 38.43350721149753 15.003661090834786 24.048542091192378 7 20.676051541125048 36.96599875120622 23.313276948402113 19.044672759266618 8 23.01199416469413 21.89885848247989 21.838688986143985 33.250458366682 9 19.585861624653038 24.380012828299456 28.19614810442009 27.837977442627416 10-11 25.907854924868158 22.607106160981395 26.10597364850673 25.379065265643714 12-13 34.462951348191204 24.397989561841406 23.79236067555717 17.346698414410223 14-15 24.605179499988648 27.26134789618293 34.5199709348532 13.613501668975225 16-17 16.465824737298757 30.731367067216357 32.86359950323521 19.939208692249675 18-19 15.106313026817752 37.72199327713944 29.838220539302657 17.33347315674015 20-21 20.68427166860284 27.181310741506813 26.95038676224747 25.18403082764287 22-23 16.341958224247165 28.94750263506851 30.167851030793468 24.542688109890857 24-25 24.715809277707887 28.812632461777337 26.604558385184678 19.86699987533009 26-27 21.236668820055986 22.749708159078804 38.185542824113426 17.828080196751785 28-29 15.579205059331544 37.514308705359674 30.157367424886377 16.749118810422402 30-31 18.236374145727787 28.491324107760136 29.121190484285925 24.151111262226152 32-33 19.119830448925004 30.146317137578908 31.980098149218545 18.75375426427754 34-35 23.414992123128535 27.633368467580155 30.030147450500376 18.921491958790927 36-37 20.5699681525053 25.48989607063117 36.83882447610304 17.101311300760486 38-39 18.765937914385773 31.587104598052886 32.78761914476443 16.85933834279691 40-41 17.339034148221184 34.397561003252754 29.858442986184308 18.404961862341754 42-43 16.016116419026893 34.27317443586866 24.387134065485704 25.323575079618738 44-45 20.067696201238732 27.03790817035837 27.230272061556445 25.664123566846452 46-47 24.38146393256928 31.501548646814843 29.02257816337017 15.094409257245708 48-49 21.25388116200927 29.52887300979683 29.638325322898503 19.578920505295393 50-51 11.71133880323658 31.282497632669724 37.67967611519684 19.326487448896852 52-53 18.815160098557087 34.97273802131623 24.663655515198943 21.548446364927738 54-55 15.213544827116877 27.88700923820272 29.34816511940476 27.551280815275643 56-57 22.251784868400527 24.5107744499315 28.273393334789404 24.964047346878573 58-59 28.750620364987313 24.989010677367666 25.56130624051728 20.699062717127745 60-61 20.239980025308842 27.88483909591824 34.89697712557385 16.97820375319907 62-63 16.00059067919588 28.822020974791062 32.64070518008616 22.5366831659269 64-65 14.941371063151296 36.23361921973723 27.706392866363473 21.118616850748005 66-67 19.89262102824016 26.560554536095047 24.512526128599507 29.03429830706529 68-69 16.502915749585895 27.184826756824215 28.854575571237323 27.457681922352567 70-71 24.619987521978334 26.59775395610005 25.814474505133006 22.967784016788613 72-73 21.180106612226382 20.917829193603264 36.06413746172167 21.83792673244868 74-75 16.11029614139425 34.373341883421816 27.986879775730422 21.52948219945351 76-77 19.130896504334444 24.655359916406148 27.74356222261975 28.470181356639664 78-79 20.818094894922158 25.733360192706545 30.28205179130267 23.166493121068623 80-81 25.04438180261016 23.753764654502554 28.338277248725262 22.863576294162023 82-83 21.404749615731472 22.046804190370317 34.50844823576789 22.03999795813033 84-85 19.535943003976335 27.71068618719972 31.00576880534111 21.74760200348284 86-87 19.264684200378802 29.95389745160083 27.820024270468284 22.961394077552086 88-89 18.379154069981794 29.24227040946552 22.92161789646234 29.456957624090347 90-91 22.178065599532527 22.694329325197927 25.703692987606836 29.423912087662714 92-93 26.014400413224315 27.571370593527476 26.62231695099489 19.79191204225332 94-95 23.44958876049377 25.25796448502146 27.616172678035923 23.67627407644884 96-97 16.041452187896166 26.466609377791766 34.441674536796754 23.050263897515308 98-99 22.38461036769856 29.8880202351887 23.254020240859997 24.47334915625275 100-101 19.579842653625345 23.765256219434402 26.866822595506413 29.788078531433836 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 982.0 1 611.5 2 411.0 3 841.5 4 685.5 5 335.5 6 557.0 7 1033.0 8 1215.0 9 889.5 10 516.5 11 298.0 12 261.0 13 242.5 14 221.0 15 215.5 16 222.0 17 217.5 18 204.0 19 213.5 20 245.0 21 289.0 22 347.5 23 424.5 24 505.0 25 602.5 26 791.5 27 1047.5 28 1287.0 29 1558.0 30 1946.0 31 2422.5 32 2945.5 33 3392.0 34 3743.0 35 3816.0 36 3580.5 37 3487.5 38 3313.5 39 2902.5 40 2567.0 41 2273.5 42 1862.5 43 1560.0 44 1352.0 45 1491.5 46 3240.0 47 13928.5 48 34380.0 49 32481.0 50 13835.5 51 5927.0 52 2909.5 53 2009.0 54 1763.0 55 1583.0 56 1301.5 57 1043.5 58 692.5 59 439.0 60 317.5 61 302.5 62 288.5 63 172.5 64 85.0 65 57.0 66 42.0 67 34.5 68 31.0 69 26.0 70 24.0 71 18.0 72 12.5 73 9.5 74 7.0 75 8.5 76 10.0 77 7.0 78 6.5 79 11.0 80 10.0 81 5.0 82 3.5 83 3.0 84 2.0 85 1.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4312445456915213 2 0.31904162841567213 3 0.16547096891185836 4 0.19607176453254452 5 0.21363888794441988 6 0.16377092471070914 7 0.16773769451339068 8 0.16830437591377376 9 0.16603765031224144 10-11 0.17510455271837067 12-13 0.16178753980936839 14-15 0.17567123411875377 16-17 0.07083517504788459 18-19 0.030034114220303065 20-21 5.666814003830766E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.1345868325909807 46-47 0.1952217424319699 48-49 0.07565196695114074 50-51 0.060634909840989196 52-53 0.06885179014654381 54-55 0.0753686262509492 56-57 0.10908616957374226 58-59 0.08981900196071764 60-61 0.13770358029308763 62-63 0.225255856652273 64-65 0.13062006278829916 66-67 0.09888590436684687 68-69 0.10313601486971996 70-71 0.08840229845975996 72-73 0.07140185644826764 74-75 0.1413870093955776 76-77 0.21335554724422834 78-79 0.13572019539174687 80-81 0.08783561705937688 82-83 0.08896897986014303 84-85 0.09803588226627226 86-87 0.06800176804596919 88-89 0.09236906826244148 90-91 0.11305293937642379 92-93 0.16462094681128375 94-95 0.13062006278829916 96-97 0.09435245316378225 98-99 0.07905205535343919 100-101 0.058651524939648425 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 176466.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.882968957192887 #Duplication Level Percentage of deduplicated Percentage of total 1 76.75315931220219 16.795870025954006 2 9.237103791174643 4.042705110332869 3 3.9336026517505696 2.58236714154568 4 2.390200952972861 2.092187730214319 5 1.5123264967888956 1.6547096891185837 6 1.0539672674539051 1.3838359797354731 7 0.8545680546923555 1.309034034884907 8 0.5852496374559768 1.0245599718926026 9 0.45059042883778744 0.887423072999898 >10 2.7734617775015535 11.203291285573425 >50 0.22529521441889372 3.313386148039849 >100 0.14242800911539258 6.561603935035644 >500 0.023306401491609695 3.7117631725091518 >1k 0.05697120364615703 27.373545045504518 >5k 0.005179200331468821 8.15964548411592 >10k+ 0.0025896001657344106 7.904072172543152 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13948 7.904072172543152 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8542 4.8405925220722406 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5857 3.3190529620436795 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4877 2.763705189668265 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3601 2.040619722779459 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3568 2.0219192365668173 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3497 1.9816848571396188 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3029 1.716477961760339 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2877 1.6303423889021114 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2780 1.575374293064953 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2425 1.3742023959289609 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2318 1.3135674860879716 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2072 1.1741638615937349 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1951 1.1055954121473826 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1682 0.9531581154443349 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1652 0.9361576734328426 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1579 0.8947899312048779 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1560 0.8840229845975995 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1417 0.8029875443428196 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1398 0.7922205977355412 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1393 0.7893871907336256 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1256 0.7117518388811442 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1176 0.6664173268504981 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1150 0.6516836104405381 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1047 0.5933154262010811 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 982 0.5564811351761813 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 970 0.5496809583715843 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 842 0.4771457391225505 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 795 0.450511713304546 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 673 0.3813765824578106 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 660 0.3740097242528306 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 589 0.33377534482563215 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 520 0.2946743281991998 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 519 0.2941076467988168 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 474 0.2686069837815783 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 474 0.2686069837815783 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 435 0.24650640916663832 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 387 0.21930570194825066 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 355 0.2011718971359922 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 349 0.19777180873369374 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 317 0.17963800392143528 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 307 0.17397118991760452 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 302 0.17113778291568915 No Hit GCTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 300 0.17000442011492298 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 299 0.1694377387145399 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 286 0.16207088050955992 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 277 0.1569707479061122 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 274 0.15527070370496301 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 260 0.1473371640995999 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 239 0.1354368546915553 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 233 0.13203676628925687 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 232 0.13147008488887377 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 230 0.13033672208810762 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 226 0.12806999648657533 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 220 0.12466990808427686 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 213 0.12070313828159533 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 208 0.11786973127967994 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 201 0.1139029614769984 No Hit GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 196 0.11106955447508303 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 187 0.10596942187163533 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 184 0.1042693776704861 No Hit TATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 184 0.1042693776704861 No Hit GTACATGGGCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAA 182 0.10313601486971996 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 181 0.10256933346933687 No Hit GTACATGGGCTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAA 177 0.10030260786780457 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.666814003830766E-4 0.0 3 0.0 0.0 0.0 5.666814003830766E-4 0.0 4 0.0 0.0 0.0 5.666814003830766E-4 0.0 5 0.0 0.0 0.0 5.666814003830766E-4 0.0 6 0.0 0.0 0.0 5.666814003830766E-4 0.0 7 0.0 0.0 0.0 5.666814003830766E-4 0.0 8 0.0 0.0 0.0 5.666814003830766E-4 0.0 9 0.0 0.0 0.0 5.666814003830766E-4 0.0 10-11 0.0 0.0 0.0 0.001983384901340768 0.0 12-13 0.0 0.0 0.0 0.009633583806512303 0.0 14-15 0.0 0.0 0.0 0.016150419910917684 0.0 16-17 0.0 0.0 0.0 0.03768431312547459 0.0 18-19 0.0 0.0 0.0 0.052984710935817664 0.0 20-21 0.0 0.0 0.0 0.08386884725669534 0.0 22-23 0.0 0.0 0.0 0.1731211678170299 0.0 24-25 0.0 0.0 0.0 0.27654052338694135 0.0 26-27 0.0 0.0 0.0 0.3329253227250575 0.0 28-29 0.0 0.0 0.0 0.38506001156030056 0.0 30-31 0.0 0.0 0.0 0.46326204481316513 0.0 32-33 0.0 0.0 0.0 0.579431731891696 0.0 34-35 0.0 0.0 0.0 0.7279022587920619 0.0 36-37 0.0 0.0 0.0 0.8967733161062188 0.0 38-39 0.0 0.0 0.0 1.1163623587546612 0.0 40-41 0.0 0.0 0.0 1.3872360681377716 0.0 42-43 0.0 0.0 0.0 1.6133419468906192 0.0 44-45 0.0 0.0 0.0 1.838597803542892 0.0 46-47 0.0 0.0 0.0 2.11258826062811 0.0 48-49 0.0 0.0 0.0 2.323110400870423 0.0 50-51 0.0 0.0 0.0 2.5562997971280588 0.0 52-53 0.0 0.0 0.0 2.772772092074394 0.0 54-55 0.0 0.0 0.0 3.015028390738159 0.0 56-57 0.0 0.0 0.0 3.3530538460666643 0.0 58-59 0.0 0.0 0.0 3.5717928666145315 0.0 60-61 0.0 0.0 0.0 3.8177325943807876 0.0 62-63 0.0 0.0 0.0 4.050638649938231 0.0 64-65 0.0 0.0 0.0 4.242460303967903 0.0 66-67 0.0 0.0 0.0 4.547334897373998 0.0 68-69 0.0 0.0 0.0 4.94004510783947 0.0 70-71 0.0 0.0 0.0 5.295921027280043 0.0 72-73 0.0 0.0 0.0 5.567644758763728 0.0 74-75 0.0 0.0 0.0 5.926354085206215 0.0 76-77 0.0 0.0 0.0 6.197511135289518 0.0 78-79 0.0 0.0 0.0 6.782326340484852 0.0 80-81 0.0 0.0 0.0 7.360624709575783 0.0 82-83 0.0 0.0 0.0 7.762968503847767 0.0 84-85 0.0 0.0 0.0 8.18174605873086 0.0 86-87 0.0 0.0 0.0 8.567939433091926 0.0 88-89 0.0 0.0 0.0 9.062935636326545 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCCATG 25 5.2483716E-5 76.04249 3 GTACCCA 30 1.2693118E-4 63.62171 1 TACCCAT 30 1.2819037E-4 63.494976 2 CGTAATG 25 0.004963884 57.145473 2 AAAGTAC 50 4.204976E-7 57.031864 3 GCGCTTG 25 0.0050031543 57.031864 9 AAGGCGC 25 0.0050031543 57.031864 6 TATATGG 45 1.4880492E-5 52.912476 2 AGTACTC 60 1.4793277E-6 47.526558 5 AAGTACT 65 2.566945E-6 43.870667 4 ATGGGGA 55 4.9298433E-5 43.20596 5 ATGGGAG 55 4.9298433E-5 43.20596 5 AAAAAGT 70 4.157453E-6 40.89967 1 TAATGAT 60 8.234991E-5 39.605465 4 AATGTAT 50 0.0016076806 38.021244 6 GAAGGCA 50 0.0016076806 38.021244 9 CGCAGAA 380 0.0 37.520966 9 GTATATG 65 1.2886898E-4 36.704834 1 TTATGTA 110 6.921073E-8 34.564766 3 TATAGGC 55 0.0025666768 34.564766 3 >>END_MODULE