##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031627_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 176466 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06149059875557 33.0 31.0 34.0 30.0 34.0 2 32.19734679768341 33.0 31.0 34.0 30.0 34.0 3 32.291517912799065 34.0 31.0 34.0 30.0 34.0 4 35.672724490836764 37.0 35.0 37.0 33.0 37.0 5 35.506284496730245 37.0 35.0 37.0 33.0 37.0 6 35.77318010268267 37.0 35.0 37.0 35.0 37.0 7 35.752150555914454 37.0 35.0 37.0 35.0 37.0 8 35.86788389831469 37.0 35.0 37.0 35.0 37.0 9 37.63519318168939 39.0 37.0 39.0 35.0 39.0 10-11 37.55653213650221 39.0 37.0 39.0 35.0 39.0 12-13 37.62946119932452 39.0 37.0 39.0 35.0 39.0 14-15 38.51487255335306 40.0 38.0 41.0 34.0 41.0 16-17 38.714514410708006 40.0 38.0 41.0 34.5 41.0 18-19 38.9329870909977 40.0 38.5 41.0 35.0 41.0 20-21 38.92710210465472 40.0 38.5 41.0 35.0 41.0 22-23 38.765303231217345 40.0 38.0 41.0 35.0 41.0 24-25 38.48981673523512 40.0 37.5 41.0 34.0 41.0 26-27 38.08662291886256 40.0 37.0 41.0 33.5 41.0 28-29 38.07565763376515 40.0 37.0 41.0 34.0 41.0 30-31 37.92340167511022 40.0 36.5 41.0 34.0 41.0 32-33 37.46903369487607 40.0 35.0 41.0 33.0 41.0 34-35 36.92344700962225 39.0 35.0 41.0 32.5 41.0 36-37 36.522196910453005 38.5 35.0 41.0 31.5 41.0 38-39 36.27168123037866 38.5 35.0 40.5 30.0 41.0 40-41 36.3221980438158 39.0 35.0 41.0 30.5 41.0 42-43 35.93640984665602 39.0 35.0 41.0 29.5 41.0 44-45 35.528042229097956 38.5 35.0 40.5 26.0 41.0 46-47 35.16018383144629 39.0 35.0 41.0 22.5 41.0 48-49 34.809317375585096 39.0 35.0 41.0 19.0 41.0 50-51 34.350169437738714 38.5 34.0 41.0 14.5 41.0 52-53 34.51286366778869 39.0 35.0 41.0 12.5 41.0 54-55 34.37927419446238 39.0 34.5 41.0 10.0 41.0 56-57 33.942683009758255 38.5 33.5 41.0 10.0 41.0 58-59 33.64826368818923 38.0 34.0 40.0 10.0 41.0 60-61 32.79328879217526 37.0 31.5 40.0 10.0 41.0 62-63 32.80460541974091 37.0 32.5 40.0 8.0 41.0 64-65 32.53789681865062 37.0 32.0 39.5 8.0 41.0 66-67 31.93348010381603 36.0 31.5 39.0 8.0 40.0 68-69 31.34104586719255 35.5 31.0 38.0 8.0 39.0 70-71 30.598866070517833 35.0 29.5 37.0 7.5 39.0 72-73 30.062493624834246 35.0 29.5 37.0 7.0 38.0 74-75 29.910810014393707 35.0 29.0 36.0 7.0 37.0 76-77 29.407554429748508 34.0 29.0 35.0 7.0 36.5 78-79 29.419103396688314 34.0 29.0 35.0 7.0 36.0 80-81 29.114962655695713 34.0 29.0 35.0 7.0 36.0 82-83 28.823555245769725 34.0 27.5 35.0 7.0 35.5 84-85 28.663408248614466 34.0 27.0 35.0 7.0 35.0 86-87 28.877285142747045 34.0 29.0 35.0 7.0 35.5 88-89 28.684491630115716 34.0 28.0 35.0 7.0 35.0 90-91 28.4295870025954 34.0 26.5 35.0 7.0 35.0 92-93 28.307543662801898 34.0 25.0 35.0 7.0 35.0 94-95 28.12390488819376 34.0 25.0 35.0 7.0 35.0 96-97 27.886859225006518 34.0 25.0 35.0 7.0 35.0 98-99 28.146319970985914 34.0 25.0 35.0 7.0 35.0 100-101 27.6733733410402 33.5 24.5 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2644073643988136 1101 2 0.20309980266510053 1101 3 0.16864539292165404 1101 4 0.4245184374400708 1101 5 0.03581578840792332 1101 6 -0.007116323637831101 1101 7 0.2551775158091587 1101 8 0.33709459487899096 1101 9 0.1916656741417313 1101 10-11 -0.19089469371699153 1101 12-13 0.2508430043823253 1101 14-15 0.28720166384876933 1101 16-17 0.43582045492882315 1101 18-19 0.24027030920776582 1101 20-21 0.26378738555821 1101 22-23 0.33014640326644695 1101 24-25 0.08556273566320982 1101 26-27 0.3385927615371642 1101 28-29 0.5855353930463494 1101 30-31 0.41925951480467916 1101 32-33 0.22329558165989738 1101 34-35 0.3613509137997184 1101 36-37 0.8047303972397231 1101 38-39 1.0256144732509256 1101 40-41 0.6625679125385204 1101 42-43 0.5510966337100385 1101 44-45 0.35495822408642397 1101 46-47 0.21606922416996355 1101 48-49 0.36869973112113286 1101 50-51 0.05381207832777335 1101 52-53 0.05053930543421359 1101 54-55 0.23109448499873508 1101 56-57 0.02035315364755519 1101 58-59 0.39263157093462553 1101 60-61 0.23268614553798983 1101 62-63 0.2394537917290549 1101 64-65 0.7705410700198456 1101 66-67 0.03964415806966315 1101 68-69 0.2556325592370854 1101 70-71 -0.15248668564681722 1101 72-73 -0.5542230085081918 1101 74-75 -0.7112609888398396 1101 76-77 -0.22311003006063856 1101 78-79 0.07430058793850947 1101 80-81 0.2042583245547256 1101 82-83 0.3586181004768747 1101 84-85 0.32162347742834285 1101 86-87 0.027359659101804823 1101 88-89 0.2444627983743466 1101 90-91 0.5483018701347859 1101 92-93 0.05836275728927376 1101 94-95 0.34762952113698375 1101 96-97 -0.3371303478114598 1101 98-99 0.10229424302991319 1101 100-101 0.2409532252779485 1102 1 0.3855947282332366 1102 2 0.41382965015965567 1102 3 0.2688632578453962 1102 4 0.5513158230609889 1102 5 0.269748250281566 1102 6 0.2213150489111939 1102 7 0.31465463999216325 1102 8 0.027780869388791984 1102 9 0.08709051074303886 1102 10-11 0.06392556772092206 1102 12-13 0.031861522900847206 1102 14-15 0.20098162027578326 1102 16-17 0.04094028063688171 1102 18-19 0.5086526621489398 1102 20-21 0.25071549013337346 1102 22-23 0.2572705862729876 1102 24-25 0.29384160276561033 1102 26-27 0.2789522386613399 1102 28-29 0.06472929282848838 1102 30-31 -0.28507055055479924 1102 32-33 -0.36371422226166317 1102 34-35 -0.48448241953361304 1102 36-37 -0.010629079884452608 1102 38-39 -0.08996286879700222 1102 40-41 -0.34410420075123227 1102 42-43 -0.2520351745688245 1102 44-45 0.36582423715831425 1102 46-47 0.2568371980261759 1102 48-49 -0.04401813380261643 1102 50-51 0.22050597810991235 1102 52-53 -0.07405219783375827 1102 54-55 -0.7227998505132405 1102 56-57 -0.17915665027400962 1102 58-59 0.10831784544443224 1102 60-61 0.33353037211751513 1102 62-63 0.5668775390057377 1102 64-65 0.8961674316756145 1102 66-67 0.8329175785489582 1102 68-69 0.26502798407368644 1102 70-71 0.32594468690220424 1102 72-73 0.4246713270037894 1102 74-75 -0.23062373393787894 1102 76-77 -0.07621460522403467 1102 78-79 -0.12858612230114375 1102 80-81 -0.15546389766749513 1102 82-83 -0.30194834832486706 1102 84-85 -0.46258131559998006 1102 86-87 -0.8984573343622486 1102 88-89 -0.6997365480308808 1102 90-91 -0.5215783041571527 1102 92-93 -0.16050979173033397 1102 94-95 -0.05542058779547787 1102 96-97 0.14917139510793476 1102 98-99 0.06438574629788718 1102 100-101 -0.2549073411708562 1103 1 0.2570544232223426 1103 2 0.00457039090039757 1103 3 0.492174804686357 1103 4 0.4612831433224258 1103 5 0.2564040237020464 1103 6 0.19141308812687896 1103 7 0.15958928051504273 1103 8 0.256212241937817 1103 9 0.36813626237702834 1103 10-11 0.5039582474594795 1103 12-13 0.5045194749705573 1103 14-15 0.5041134285546534 1103 16-17 0.8255263372817652 1103 18-19 0.46453501509011375 1103 20-21 0.5726109149699781 1103 22-23 0.418381697384099 1103 24-25 0.6995333238985069 1103 26-27 0.4893280556548021 1103 28-29 0.3723000989287044 1103 30-31 0.6435242206870342 1103 32-33 0.7122661698951944 1103 34-35 0.28782150203500834 1103 36-37 0.4812009854750201 1103 38-39 0.4226732967803457 1103 40-41 0.9677149713620565 1103 42-43 0.8488907513570965 1103 44-45 1.097605282909953 1103 46-47 0.7087162829934996 1103 48-49 0.3503173781799589 1103 50-51 0.8038120783277733 1103 52-53 0.7968628348459745 1103 54-55 0.8046238967634451 1103 56-57 0.8438825654122653 1103 58-59 1.0360139238758066 1103 60-61 1.045186145537997 1103 62-63 0.5887184976114028 1103 64-65 0.7999528347257225 1103 66-67 0.8190559227755472 1103 68-69 0.8769560886488499 1103 70-71 0.6306015496473023 1103 72-73 0.2582769914918046 1103 74-75 0.5056507758660409 1103 76-77 0.40924291111583244 1103 78-79 0.5118005879385095 1103 80-81 1.068228912790019 1103 82-83 0.6564122181239327 1103 84-85 0.27750583036952037 1103 86-87 0.25162436498415985 1103 88-89 0.6378451513155241 1103 90-91 0.36815481131125694 1103 92-93 0.3046862867010347 1103 94-95 -0.05678224356889672 1103 96-97 0.38713435807089525 1103 98-99 0.4625883606769712 1103 100-101 0.5350708723367674 1104 1 0.04300213564064137 1104 2 0.09484816867817614 1104 3 3.447393268842802E-4 1104 4 0.1402047119498775 1104 5 -0.0033998978665863433 1104 6 0.23103727113340966 1104 7 0.46187686221438895 1104 8 0.5388919804999048 1104 9 0.5662571774097032 1104 10-11 0.04031445660980637 1104 12-13 0.06681522660455386 1104 14-15 0.4616297684239399 1104 16-17 0.4425606510072626 1104 18-19 0.16143043992671835 1104 20-21 0.12224326791115203 1104 22-23 0.13018725293965616 1104 24-25 0.29430456572856656 1104 26-27 0.32756334977245416 1104 28-29 0.390886700235896 1104 30-31 0.7885405605563207 1104 32-33 0.7647579999605512 1104 34-35 0.7814898026886041 1104 36-37 0.8053431423377617 1104 38-39 0.28480564972151967 1104 40-41 -0.21100234889938463 1104 42-43 -0.17541480419846067 1104 44-45 0.12762979271386854 1104 46-47 0.49711497580395303 1104 48-49 0.08908371804924542 1104 50-51 0.16819116329509853 1104 52-53 0.00233669105512746 1104 54-55 0.40266311244971575 1104 56-57 0.46459334972599464 1104 58-59 0.15345673433331797 1104 60-61 0.455521112858257 1104 62-63 0.05113679826499862 1104 64-65 0.165148913157104 1104 66-67 -0.1286564955251066 1104 68-69 0.31363909518479716 1104 70-71 0.4294169091244271 1104 72-73 0.06934725292971677 1104 74-75 -0.041734845048988944 1104 76-77 0.2932298392204089 1104 78-79 -0.20266019637521637 1104 80-81 0.1278694356658363 1104 82-83 0.4100886887121682 1104 84-85 0.4184372029185397 1104 86-87 -0.0672073343622479 1104 88-89 0.04818011863578775 1104 90-91 0.06198651065766114 1104 92-93 0.4533790971585532 1104 94-95 -0.11907799520288265 1104 96-97 0.07463435807089525 1104 98-99 -0.2800586981465578 1104 100-101 0.5119908069772912 1105 1 0.1203294629679732 1105 2 0.13294876927877652 1105 3 -0.04319880421666511 1105 4 0.35416867591384005 1105 5 0.0697983003316125 1105 6 0.2019456795418222 1105 7 0.43522521056273433 1105 8 0.22958267119059883 1105 9 0.3363397599922848 1105 10-11 0.43539703919238804 1105 12-13 0.22212678816610776 1105 14-15 0.21406970836387273 1105 16-17 0.23450884920546144 1105 18-19 0.07959860809489072 1105 20-21 0.20416894358682924 1105 22-23 0.2528411568435587 1105 24-25 0.3361589200829229 1105 26-27 0.714387674096777 1105 28-29 0.3808454089446016 1105 30-31 0.5916561761719308 1105 32-33 0.5743488408013917 1105 34-35 0.5691211840699921 1105 36-37 0.5235300792747068 1105 38-39 0.17247456864043897 1105 40-41 0.715330233683197 1105 42-43 0.22389075135709646 1105 44-45 0.33737078370486273 1105 46-47 0.47252788871686136 1105 48-49 0.4943952796107993 1105 50-51 0.6818923644962993 1105 52-53 0.3197165709350074 1105 54-55 0.5102081574947661 1105 56-57 0.42377127765391975 1105 58-59 0.24457910670569305 1105 60-61 0.2133101518972964 1105 62-63 0.4257614228896287 1105 64-65 0.31436062486881156 1105 66-67 0.8092188798502633 1105 68-69 0.419682638728343 1105 70-71 0.6207645067220255 1105 72-73 0.8524741298065912 1105 74-75 0.6324393291251837 1105 76-77 0.5743859953765664 1105 78-79 0.274817281102262 1105 80-81 0.4863529191493221 1105 82-83 0.44387247249595063 1105 84-85 0.09805432003565429 1105 86-87 0.12076188485696804 1105 88-89 -0.02849030178463252 1105 90-91 -9.827085615583542E-4 1105 92-93 0.5048055235849809 1105 94-95 0.435069902695016 1105 96-97 0.6641613850979233 1105 98-99 0.233454815366958 1105 100-101 0.584156722893205 1106 1 0.12911324675175706 1106 2 0.017070390900400412 1106 3 -0.01701637178422999 1106 4 -0.03687862138345821 1106 5 -0.2888165645332492 1106 6 -0.47035161775547607 1106 7 -0.34298424889672674 1106 8 0.019447536055459125 1106 9 0.10306273296526314 1106 10-11 0.16774501216536208 1106 12-13 -0.07728199561767468 1106 14-15 -0.1793424537982915 1106 16-17 -0.4022310156594102 1106 18-19 -0.2913473378510574 1106 20-21 -0.2652567320888508 1106 22-23 -0.05036830261590097 1106 24-25 -0.19145932316031633 1106 26-27 -0.30368665022754726 1106 28-29 -0.04001607754188541 1106 30-31 -0.014931661665904983 1106 32-33 -0.4116308889283289 1106 34-35 -0.9167740862002844 1106 36-37 -0.021740190995565456 1106 38-39 0.11605564972151683 1106 40-41 0.028928206656175348 1106 42-43 0.23326575135710215 1106 44-45 0.20054645938053994 1106 46-47 0.8373927535817316 1106 48-49 0.5981114958270197 1106 50-51 0.7720106077395386 1106 52-53 1.001642246610686 1106 54-55 0.9380797791163928 1106 56-57 0.7510516830593232 1106 58-59 1.1999845121110937 1106 60-61 1.0187155573026985 1106 62-63 0.9216229093761186 1106 64-65 0.8828572464904383 1106 66-67 1.0914823933637763 1106 68-69 0.4747502062959086 1106 70-71 0.28115302023553923 1106 72-73 0.3044166973741582 1106 74-75 0.17458459939545534 1106 76-77 0.25538261699818676 1106 78-79 -0.06411852970854781 1106 80-81 0.2667583245547256 1106 82-83 0.20383868871216748 1106 84-85 -0.073229463748131 1106 86-87 -0.4248462232511372 1106 88-89 -0.2855004369197722 1106 90-91 0.029000399546550426 1106 92-93 0.21240687493633104 1106 94-95 0.3218942270193388 1106 96-97 -0.25661564192910546 1106 98-99 -0.2956836981465578 1106 100-101 -0.0959953041338224 1107 1 0.26260381278949296 1107 2 0.10677164876203449 1107 3 0.24300721312143025 1107 4 0.017563202515916032 1107 5 -0.010475369564694859 1107 6 -0.11579186932780061 1107 7 0.10626889575735987 1107 8 0.2516802404579792 1107 9 0.3281806574935615 1107 10-11 0.10648321971252983 1107 12-13 -0.0388582534793116 1107 14-15 -0.010199372037284604 1107 16-17 0.021373544089023255 1107 18-19 0.055822473469696376 1107 20-21 0.23289578363442587 1107 22-23 0.5425955338620909 1107 24-25 0.42074586551892423 1107 26-27 0.39635265794855457 1107 28-29 0.11762936270968538 1107 30-31 0.46608248927748974 1107 32-33 0.6803109349710326 1107 34-35 0.8098572031078888 1107 36-37 0.7933541486270741 1107 38-39 1.0342160270800065 1107 40-41 1.0947497475366816 1107 42-43 1.7160291161369727 1107 44-45 1.174209981393119 1107 46-47 1.879059420248403 1107 48-49 1.966114640481102 1107 50-51 1.1004500731483446 1107 52-53 1.590518032774206 1107 54-55 1.6074587099339936 1107 56-57 1.8315351736253618 1107 58-59 1.7152360844381391 1107 60-61 2.0369545510133875 1107 62-63 2.1347321861056727 1107 64-65 1.9273344477482937 1107 66-67 1.2276066072002578 1107 68-69 1.496684168560062 1107 70-71 1.2561333661474876 1107 72-73 1.1409536470596962 1107 74-75 0.9813259515967125 1107 76-77 0.8359447239164268 1107 78-79 0.9828743948197527 1107 80-81 0.7761922868188762 1107 82-83 0.5854031541209714 1107 84-85 0.9282642469436944 1107 86-87 1.0346663553652178 1107 88-89 0.30571418572174025 1107 90-91 0.8280373492320869 1107 92-93 0.9363455541816137 1107 94-95 0.24367095657908422 1107 96-97 -0.03189079916180759 1107 98-99 0.018683440218222103 1107 100-101 0.5689221486963696 1108 1 0.17077991341842136 1108 2 0.38487342120343015 1108 3 0.18468817367031676 1108 4 0.34929562104078116 1108 5 0.6306910112243216 1108 6 0.415254442850582 1108 7 0.43182635716387807 1108 8 0.3368717784797042 1108 9 0.2850703087228368 1108 10-11 0.2659457697411227 1108 12-13 0.03455512559444429 1108 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-0.8175379041801349 2316 94-95 -0.9293607930609831 2316 96-97 -1.2830463648206702 2316 98-99 -0.8423076941304934 2316 100-101 -1.0046172920856264 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 3.0 13 20.0 14 112.0 15 366.0 16 867.0 17 1910.0 18 2562.0 19 2912.0 20 3517.0 21 4250.0 22 3437.0 23 2450.0 24 2422.0 25 2449.0 26 2495.0 27 2620.0 28 2975.0 29 3120.0 30 3309.0 31 3794.0 32 4773.0 33 6182.0 34 8440.0 35 15372.0 36 23344.0 37 38116.0 38 31621.0 39 3025.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.567083368767186 17.37279247101511 13.592425659778327 13.46769850043938 2 14.182415776499587 31.940044768084324 29.662539313744933 24.215000141671155 3 33.84353837871533 25.2548664041028 30.13628764910889 10.76530756807299 4 9.79439866710492 16.841401353296533 44.63271713382221 28.73148284577633 5 7.748234787437807 46.706447700973555 33.2273639114617 12.317953600126936 6 22.265478902451463 40.00544014144368 14.124533904548183 23.604547051556672 7 21.501592374735075 36.56455067831764 23.03276551857015 18.901091428377136 8 23.25377126471955 22.028039395690953 21.28115330998606 33.43703602960343 9 19.151564607346458 24.86201307900672 27.379778540908728 28.60664377273809 10-11 26.092845080638767 22.707773735450452 26.582174469869553 24.61720671404123 12-13 35.500889689798605 23.880237552843038 23.63344780297621 16.985424954382147 14-15 24.14686115172328 27.68946992621808 34.63528385071345 13.52838507134519 16-17 16.369158931465552 30.01343034918908 33.26476488388698 20.35264583545839 18-19 15.128693346026997 37.41995625219589 29.863826459487946 17.587523942289167 20-21 21.026146679813674 25.837271769065996 27.85664093933109 25.279940611789243 22-23 16.580482649386415 28.501928160781127 30.557027373932854 24.360561815899608 24-25 24.853512858000975 28.658778461573338 27.82972357281289 18.6579851076128 26-27 21.789749866829872 21.73223170469099 39.239286888125754 17.238731540353385 28-29 15.165840510827886 37.24531585227256 30.761338740898825 16.827504896000725 30-31 18.68150553907107 26.745112042555487 30.273453552005485 24.299928866367964 32-33 19.42640508653225 29.347863044439155 32.82445343578933 18.401278433239266 34-35 23.55807917672526 26.522389582129136 32.01239898904038 17.90713225210522 36-37 21.020763206510036 24.291931590221346 37.71944737229835 16.967857830970274 38-39 18.05588611970578 30.95865492502805 34.385660693844706 16.599798261421466 40-41 17.33931748892138 33.08257681366382 30.42569106796777 19.15241462944703 42-43 16.29747373431709 33.1534119887117 25.485929300828484 25.06318497614271 44-45 20.950778053562725 25.43067786429114 28.245951061394265 25.37259302075187 46-47 25.16972107941473 30.515226729228296 29.526084344859633 14.788967846497341 48-49 21.980040304865778 28.871548789578554 30.280235026685677 18.868175878869987 50-51 11.732068371418208 30.644722524483132 38.35209240116068 19.271116702937977 52-53 19.30286854124874 34.34769304001904 25.446828284202056 20.902610134530164 54-55 15.443049451872756 27.27035749400021 29.26112617551972 28.025466878607315 56-57 22.683406435233984 23.8581369782281 28.916335158047442 24.542121428490475 58-59 29.4133147461834 24.18227873924722 26.040993732503715 20.36341278206567 60-61 19.696712114514977 28.434655967721827 35.1928983486904 16.675733569072797 62-63 15.381433250597848 28.63441116135686 32.95082338807476 23.033332199970534 64-65 14.138700939557761 37.072013872360685 27.174073192569676 21.61521199551188 66-67 19.884000317341584 26.123162535559256 24.66594131447418 29.326895832624984 68-69 16.238538868677253 27.443813539152018 28.240567588090627 28.077080004080106 70-71 24.08820962678363 27.014552378361838 25.852005485475953 23.04523250937858 72-73 20.878242834313692 20.553817732594382 36.35034510897283 22.217594324119094 74-75 14.895220609069169 35.02176056577471 27.66595264810218 22.417066177053936 76-77 18.59735019777181 24.136944227216574 27.457413892761213 29.808291682250402 78-79 19.545408180612696 26.037593644101413 30.016547096891188 24.400451078394706 80-81 23.32477513474912 23.382868866860502 28.99444000476085 24.297915993629527 82-83 19.613043626100854 21.948779851972752 34.67617622723203 23.762000294694367 84-85 16.647682839753834 27.881574920947944 31.477168406378564 23.993573832919658 86-87 16.838654471682933 29.394330919270566 27.372411682703753 26.394602926342753 88-89 15.50496979588136 29.50341708884431 22.928779481599854 32.06283363367447 90-91 19.82619881450251 22.147609171171784 25.807520995545886 32.21867101877982 92-93 23.0109482846554 27.63591853388188 26.436820690671293 22.916312490791427 94-95 20.006120159124137 25.602949010007592 27.960060294901 26.430870535967266 96-97 11.853558192513006 26.70939444425555 34.870739972572615 26.56630739065882 98-99 18.286525449661692 29.91936123672549 23.777101537973323 28.0170117756395 100-101 14.6263302845874 24.12136048870604 27.104088039622365 34.1482211870842 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 866.0 1 552.0 2 408.0 3 923.0 4 777.0 5 326.5 6 522.5 7 1004.0 8 1221.0 9 963.5 10 574.0 11 275.0 12 205.0 13 212.5 14 232.5 15 202.0 16 171.5 17 193.0 18 212.0 19 218.0 20 255.5 21 306.0 22 361.0 23 424.0 24 494.0 25 595.5 26 742.5 27 968.5 28 1169.0 29 1368.0 30 1784.5 31 2273.0 32 2809.0 33 3342.5 34 3736.5 35 3757.0 36 3477.5 37 3404.5 38 3274.5 39 2995.5 40 2723.5 41 2409.5 42 2085.0 43 1824.5 44 1595.0 45 1662.0 46 3028.0 47 14131.0 48 35052.0 49 32929.0 50 14284.5 51 6240.5 52 2774.5 53 1522.0 54 1359.0 55 1344.0 56 1193.0 57 982.0 58 632.0 59 411.0 60 289.0 61 256.5 62 220.0 63 117.5 64 58.0 65 32.5 66 21.0 67 21.5 68 19.5 69 11.5 70 7.0 71 6.0 72 6.5 73 5.0 74 3.5 75 6.0 76 6.5 77 3.5 78 11.0 79 12.0 80 3.0 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.045901193431029205 2 5.666814003830766E-4 3 5.666814003830766E-4 4 0.004533451203064613 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0014167035009576916 24-25 0.0 26-27 0.0 28-29 0.026350685117813064 30-31 0.021250552514365372 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.03428422472317614 50-51 0.01020026520689538 52-53 0.0 54-55 8.500221005746149E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.015300397810343069 82-83 0.006800176804596919 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 176466.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.798907438260063 #Duplication Level Percentage of deduplicated Percentage of total 1 75.57147917064 15.718042002425397 2 9.462441762253766 3.93616900706085 3 4.337520093725308 2.706470368229574 4 2.487535078876386 2.069520474198996 5 1.5557311391439392 1.617875398093684 6 1.0952783151241043 1.366835537723981 7 0.828270168651064 1.2058980200151872 8 0.621202626488298 1.0336268742987318 9 0.4604528240198349 0.8619224099826595 >10 3.097839413671907 11.83230763999864 >50 0.21524126093234885 2.904808858363651 >100 0.17164809416124022 6.97528135731529 >500 0.024521156308748605 3.512858000974692 >1k 0.06266517723346865 28.06149626556957 >5k 0.005449145846388578 7.203087280269287 >10k+ 0.002724572923194289 8.99380050547981 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 15871 8.99380050547981 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7553 4.280144617093378 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5158 2.9229426631759092 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4223 2.3930955538177328 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3873 2.1947570636836558 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3583 2.0304194575725636 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3251 1.8422812326453823 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3179 1.8014801718178006 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3104 1.75897906678907 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2585 1.464871419990253 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2489 1.4104700055534778 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2259 1.28013328346537 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2243 1.2710663810592409 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1981 1.1225958541588748 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1874 1.0619609443178857 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1712 0.9701585574558271 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1635 0.9265240896263303 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1492 0.8454886493715502 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1418 0.8035542257432027 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1413 0.8007208187412873 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1373 0.7780535627259642 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1301 0.7372525018983828 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1234 0.6992848480727166 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1181 0.6692507338524135 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1062 0.6018156472068273 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1054 0.5972821960037628 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 909 0.5151133929482166 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 854 0.4839459159271474 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 803 0.4550451645076105 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 654 0.37060963585053214 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 644 0.36494282184670135 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 643 0.3643761404463183 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 609 0.34510897283329367 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 553 0.3133748144118414 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 530 0.3003411422030306 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 500 0.28334070019153834 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 446 0.25273990457085216 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 427 0.2419729579635737 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 403 0.2283726043543799 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 358 0.20287194133714143 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 354 0.20060521573560913 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 344 0.19493840173177837 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 334 0.18927158772794758 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 306 0.17340450851722145 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 291 0.1649042875114753 RNA PCR Primer, Index 39 (95% over 23bp) GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 277 0.1569707479061122 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 270 0.1530039781034307 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 266 0.1507372525018984 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 264 0.14960388970113223 No Hit GCTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 263 0.14903720830074915 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 259 0.14677048269921686 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 257 0.14563711989845068 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 232 0.13147008488887377 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 230 0.13033672208810762 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 230 0.13033672208810762 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 223 0.12636995228542608 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 208 0.11786973127967994 No Hit TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG 208 0.11786973127967994 RNA PCR Primer, Index 39 (96% over 30bp) TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 197 0.1116362358754661 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 190 0.10766946607278455 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 186 0.10540274047125225 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.666814003830766E-4 0.0 3 0.0 0.0 0.0 5.666814003830766E-4 0.0 4 0.0 0.0 0.0 5.666814003830766E-4 0.0 5 0.0 0.0 0.0 5.666814003830766E-4 0.0 6 0.0 0.0 0.0 5.666814003830766E-4 0.0 7 0.0 0.0 0.0 5.666814003830766E-4 0.0 8 0.0 0.0 0.0 5.666814003830766E-4 0.0 9 0.0 0.0 0.0 5.666814003830766E-4 0.0 10-11 0.0 0.0 0.0 0.0014167035009576916 0.0 12-13 0.0 0.0 0.0 0.008783561705937687 0.0 14-15 0.0 0.0 0.0 0.013883694309385377 0.0 16-17 0.0 0.0 0.0 0.035984268924325366 0.0 18-19 0.0 0.0 0.0 0.05128466673466843 0.0 20-21 0.0 0.0 0.0 0.08216880305554611 0.0 22-23 0.0 0.0 0.0 0.17170446431607223 0.0 24-25 0.0 0.0 0.0 0.2768238640871329 0.0 26-27 0.0 0.0 0.0 0.33434202622601517 0.0 28-29 0.0 0.0 0.0 0.3890267813629821 0.0 30-31 0.0 0.0 0.0 0.4660954518150805 0.0 32-33 0.0 0.0 0.0 0.5845318644951436 0.0 34-35 0.0 0.0 0.0 0.7341357541962757 0.0 36-37 0.0 0.0 0.0 0.9015901080094749 0.0 38-39 0.0 0.0 0.0 1.1223125134586833 0.0 40-41 0.0 0.0 0.0 1.393186222841794 0.0 42-43 0.0 0.0 0.0 1.6130586061904277 0.0 44-45 0.0 0.0 0.0 1.8388811442430835 0.0 46-47 0.0 0.0 0.0 2.112871601328301 0.0 48-49 0.0 0.0 0.0 2.3228270601702308 0.0 50-51 0.0 0.0 0.0 2.5528997087257603 0.0 52-53 0.0 0.0 0.0 2.76653859667018 0.0 54-55 0.0 0.0 0.0 3.0121949837362436 0.0 56-57 0.0 0.0 0.0 3.35220382396609 0.0 58-59 0.0 0.0 0.0 3.5734929108156814 0.0 60-61 0.0 0.0 0.0 3.8177325943807876 0.0 62-63 0.0 0.0 0.0 4.05403873834053 0.0 64-65 0.0 0.0 0.0 4.246143733070394 0.0 66-67 0.0 0.0 0.0 4.553568392778212 0.0 68-69 0.0 0.0 0.0 4.945995262543493 0.0 70-71 0.0 0.0 0.0 5.302154522684257 0.0 72-73 0.0 0.0 0.0 5.577561683270432 0.0 74-75 0.0 0.0 0.0 5.933154262010812 0.0 76-77 0.0 0.0 0.0 6.205161334194688 0.0 78-79 0.0 0.0 0.0 6.791393242890981 0.0 80-81 0.0 0.0 0.0 7.368841589881336 0.0 82-83 0.0 0.0 0.0 7.769768680652364 0.0 84-85 0.0 0.0 0.0 8.187412872734692 0.0 86-87 0.0 0.0 0.0 8.575022950596715 0.0 88-89 0.0 0.0 0.0 9.07681933063593 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCATGC 15 6.6125614E-4 94.98556 8 GCTAAAC 15 6.6125614E-4 94.98556 3 GTACCCA 25 5.252147E-5 76.03153 1 AGGTGGA 20 0.0020724095 71.23916 6 AAGGCAG 30 1.2991652E-4 63.323704 9 ACCCATG 30 1.2991652E-4 63.323704 3 CATGGAC 40 7.5259177E-6 59.365967 4 ACTATAC 25 0.005017273 56.99133 3 TAGAACG 25 0.005017273 56.99133 6 AGAACGT 25 0.005017273 56.99133 7 GAACGTC 25 0.005017273 56.99133 8 ATGGACT 35 2.783555E-4 54.277454 5 GGACTAA 40 5.3797464E-4 47.492775 7 CTAAACC 50 2.8197326E-5 47.492775 4 AATGTAT 45 9.6100976E-4 42.215797 6 GACTAAA 45 9.6100976E-4 42.215797 8 ATGGGAG 60 8.26964E-5 39.577316 5 GCTTTGC 100 2.994966E-8 38.015766 1 GAGGACT 25 0.0016155057 37.99422 12-13 CTTTGCG 100 3.0098818E-8 37.99422 2 >>END_MODULE