FastQCFastQC Report
Fri 10 Feb 2017
SRR2031626_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031626_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069142
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19420.18164097940217483No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC352.779286E-454.319553
CATACCG1004.9317874E-528.5177655
GACCGTA1004.941253E-528.5097457
GTACATG32800.027.309161
TACATGG32550.026.8992652
ACATGGG34300.024.24983
CATGGGG22350.022.5420074
CCGTATA1302.9317362E-421.9336579
GTATTAT3201.6370905E-1120.8450241
GTATCCG1150.003657587320.7155511
GAGTACA24900.020.2829381
CCTACAC5400.020.2440933
GGACCGT2151.0746298E-619.8914536
ACCGTAT1456.125704E-419.6646588
AGTACAT24700.019.457992
GTGTATA6950.019.1954171
TATGACG1250.006009199719.0118434
TATACCC2752.8165232E-819.0118435
ATTATAC4450.018.157383
AGAGTAC28500.017.84110310-11
Produced by FastQC (version 0.11.5)