##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031626_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1069142 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.83919909609762 33.0 31.0 34.0 30.0 34.0 2 32.091578106556476 34.0 31.0 34.0 30.0 34.0 3 32.24825981955624 34.0 31.0 34.0 30.0 34.0 4 35.322863567234286 37.0 35.0 37.0 33.0 37.0 5 35.00652018160357 37.0 35.0 37.0 32.0 37.0 6 35.2284897609485 37.0 35.0 37.0 32.0 37.0 7 35.37925083852285 37.0 35.0 37.0 33.0 37.0 8 35.46083588522385 37.0 35.0 37.0 33.0 37.0 9 37.39641881059766 39.0 38.0 39.0 34.0 39.0 10-11 37.343047041459414 39.0 37.5 39.0 34.5 39.0 12-13 37.360413303377854 39.0 38.0 39.0 34.5 39.0 14-15 38.775354910760214 41.0 39.0 41.0 35.0 41.0 16-17 38.72425692751758 41.0 38.0 41.0 34.5 41.0 18-19 38.63104059142752 41.0 38.5 41.0 34.0 41.0 20-21 38.61178496401787 40.0 38.0 41.0 34.0 41.0 22-23 38.55184203782098 40.0 38.0 41.0 34.0 41.0 24-25 38.46577021574309 40.0 38.0 41.0 34.0 41.0 26-27 38.36340168097409 40.0 38.0 41.0 34.0 41.0 28-29 38.24598509833118 40.0 38.0 41.0 33.5 41.0 30-31 38.11250890901302 40.0 38.0 41.0 33.0 41.0 32-33 37.961843702707405 40.0 38.0 41.0 33.0 41.0 34-35 37.82084699693773 40.0 38.0 41.0 33.0 41.0 36-37 37.69358700715153 40.0 38.0 41.0 32.0 41.0 38-39 37.518621006377074 40.0 37.0 41.0 31.0 41.0 40-41 37.365152617706535 40.0 37.0 41.0 31.0 41.0 42-43 37.18845812810646 40.0 37.0 41.0 31.0 41.0 44-45 36.968311505861706 40.0 36.5 41.0 30.0 41.0 46-47 36.790847240123384 39.5 36.0 41.0 30.0 41.0 48-49 36.944888050418 40.0 36.5 41.0 30.0 41.0 50-51 36.706650753594936 39.5 36.0 40.5 30.0 41.0 52-53 36.828134616355925 39.5 36.0 40.5 30.5 41.0 54-55 37.085683660355684 40.0 36.0 41.0 31.0 41.0 56-57 36.95560552293334 40.0 36.0 41.0 30.5 41.0 58-59 36.73349330584712 39.0 35.5 41.0 30.0 41.0 60-61 36.40900694201518 39.0 35.0 41.0 29.0 41.0 62-63 36.047552149293544 39.0 35.0 41.0 28.5 41.0 64-65 35.7433437279613 38.0 35.0 40.5 28.0 41.0 66-67 35.485396701280095 37.0 35.0 40.0 28.0 41.0 68-69 35.201543387127245 37.0 34.5 39.5 28.0 41.0 70-71 34.86626378909443 36.0 34.0 39.0 28.0 41.0 72-73 34.47981231679234 36.0 34.0 39.0 27.5 41.0 74-75 34.06186035157164 35.0 34.0 37.5 26.0 40.0 76-77 33.67846600358044 35.0 34.0 37.0 26.0 39.0 78-79 33.33954376500034 35.0 33.5 37.0 26.0 39.0 80-81 33.03169925042698 35.0 33.0 36.0 26.0 38.5 82-83 32.73218992425702 35.0 33.0 36.0 25.5 37.0 84-85 32.50678675049713 35.0 33.0 35.5 25.0 37.0 86-87 32.30794459482463 35.0 33.0 35.0 25.0 36.5 88-89 32.09696373353586 35.0 32.5 35.0 24.5 36.0 90-91 31.917341195089147 35.0 32.0 35.0 24.0 36.0 92-93 31.758352491998256 35.0 32.0 35.0 24.0 36.0 94-95 31.617709808425822 35.0 32.0 35.0 23.5 35.0 96-97 31.51749814337104 35.0 32.0 35.0 23.0 35.0 98-99 31.414874731326616 35.0 32.0 35.0 23.0 35.0 100-101 30.948783229917076 34.5 31.0 35.0 19.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2897393757462048 1101 2 0.335651749302599 1101 3 0.33012277252419153 1101 4 0.2622720045248812 1101 5 0.2504296198681999 1101 6 0.35079669711682726 1101 7 0.3977239519514626 1101 8 0.36468683448641315 1101 9 0.512687169627668 1101 10-11 0.5193744605533013 1101 12-13 0.5310198631316041 1101 14-15 0.4956777511053474 1101 16-17 0.542161645219565 1101 18-19 0.5402388336683615 1101 20-21 0.2771481876451176 1101 22-23 0.3744062788263136 1101 24-25 0.47458906749273666 1101 26-27 0.36147542548725653 1101 28-29 0.44139379681001145 1101 30-31 0.5897963857656521 1101 32-33 0.4647372077218961 1101 34-35 0.46414001985751696 1101 36-37 0.3534921023578832 1101 38-39 0.48298695862376206 1101 40-41 0.4409697582574026 1101 42-43 0.47421356215148336 1101 44-45 0.4816611332262184 1101 46-47 0.5093516148089634 1101 48-49 0.7465571840456136 1101 50-51 0.569583620347828 1101 52-53 0.5727185463531939 1101 54-55 0.4727523507462692 1101 56-57 0.5722288318557887 1101 58-59 0.5843047508379442 1101 60-61 0.48625304962309457 1101 62-63 0.4028641824953212 1101 64-65 0.410208995416383 1101 66-67 0.4628088760937459 1101 68-69 0.3284772613392235 1101 70-71 0.4231967330756774 1101 72-73 0.4367566116855457 1101 74-75 0.40133623474406477 1101 76-77 0.47192676240761955 1101 78-79 0.389057537264371 1101 80-81 0.6222620038535212 1101 82-83 0.48605985085546166 1101 84-85 0.46365789381969336 1101 86-87 0.2950735575558099 1101 88-89 0.5435636670554018 1101 90-91 0.5604509981726231 1101 92-93 0.5395213992812842 1101 94-95 0.43770977830744684 1101 96-97 0.4487456348839416 1101 98-99 0.5397720463261493 1101 100-101 0.6607756152491788 1102 1 -0.3024148358247274 1102 2 -0.18244552709797546 1102 3 -0.12306532466785569 1102 4 -0.14396259305700454 1102 5 -0.0413718650303494 1102 6 -0.001269591878028109 1102 7 0.004595795397221991 1102 8 -3.5454190680894726E-4 1102 9 0.025752035424027042 1102 10-11 0.017309557399485698 1102 12-13 0.15689640759119783 1102 14-15 0.02093621045982985 1102 16-17 0.02692040644638638 1102 18-19 0.06946381110325461 1102 20-21 -0.045944382921312865 1102 22-23 0.02148016584531831 1102 24-25 0.23734633683154271 1102 26-27 0.1742579412346288 1102 28-29 0.19716040189712913 1102 30-31 0.35370631332834535 1102 32-33 0.32719308676087167 1102 34-35 0.09433255535613938 1102 36-37 0.16815213144849395 1102 38-39 -0.05996025529346127 1102 40-41 0.3042065285970281 1102 42-43 0.11964010594827101 1102 44-45 -0.05403692695784912 1102 46-47 -0.79732416225621 1102 48-49 0.0037951907964668408 1102 50-51 0.10239431084614381 1102 52-53 0.0481186627638337 1102 54-55 0.039072075120564875 1102 56-57 -0.1808154993915494 1102 58-59 -0.10035254272295191 1102 60-61 -0.3704015783026833 1102 62-63 -0.8108288910711394 1102 64-65 -0.3414452770110401 1102 66-67 -0.2294458390797729 1102 68-69 -0.2455706357373728 1102 70-71 -0.19382970997650517 1102 72-73 -0.18954472318064575 1102 74-75 -0.26267624505579334 1102 76-77 -0.2101414185615269 1102 78-79 0.07698782584467523 1102 80-81 -0.158997448486204 1102 82-83 -0.3217511107856623 1102 84-85 0.047106010943743115 1102 86-87 -0.2941016983126232 1102 88-89 -0.19099655737854704 1102 90-91 -0.30829057380494973 1102 92-93 -0.2474049378444363 1102 94-95 -0.18973397160970507 1102 96-97 -0.27234300953455204 1102 98-99 -0.1826680943788972 1102 100-101 -0.19973526601316394 1103 1 0.1458224156635346 1103 2 0.09602217105620525 1103 3 0.06964710372707117 1103 4 0.024780854844451028 1103 5 0.13969537822098488 1103 6 0.1594802958002859 1103 7 0.3086119978009023 1103 8 0.19418662399233 1103 9 0.3496690671361833 1103 10-11 0.26900549790643424 1103 12-13 0.2775269768749524 1103 14-15 0.4492392871381057 1103 16-17 0.40118622040422025 1103 18-19 0.4845404238555062 1103 20-21 0.2512137613371763 1103 22-23 0.18541308639644427 1103 24-25 0.34977683778627977 1103 26-27 -0.14264834503590862 1103 28-29 0.23647182314090998 1103 30-31 0.20491751554482107 1103 32-33 0.09343819608731252 1103 34-35 0.046683003706874615 1103 36-37 0.1786079937046665 1103 38-39 0.051496206011712786 1103 40-41 -0.0740028296717199 1103 42-43 -0.12544582014405847 1103 44-45 0.03140770312938912 1103 46-47 0.17938897750941152 1103 48-49 0.2118717052672423 1103 50-51 0.11844695227787128 1103 52-53 -0.0925414377640763 1103 54-55 0.012874796113486298 1103 56-57 0.04356755032952009 1103 58-59 0.05066396856537381 1103 60-61 0.1736220888132678 1103 62-63 0.34548779993188816 1103 64-65 0.3282107846161253 1103 66-67 0.2159447595948336 1103 68-69 0.15520953199634846 1103 70-71 0.07550834086777769 1103 72-73 0.15775686523524968 1103 74-75 0.01925119513290241 1103 76-77 -0.551213833290646 1103 78-79 -0.32948974257794816 1103 80-81 -0.09643836259754579 1103 82-83 -0.2363720016838613 1103 84-85 -0.19818993330726187 1103 86-87 -0.15595128553041349 1103 88-89 -0.06321484054152648 1103 90-91 0.0015610913640919932 1103 92-93 -0.1265614253580054 1103 94-95 -0.32202318123941964 1103 96-97 0.055436835274310425 1103 98-99 0.07948546341061302 1103 100-101 0.05580125725614238 1104 1 -0.057909435516137364 1104 2 0.07832480263515151 1104 3 0.15712668630420268 1104 4 -0.024831214121064704 1104 5 -0.1612392860258396 1104 6 -0.037645384780482516 1104 7 0.04259339526007011 1104 8 0.2497946094732697 1104 9 0.3087979237605012 1104 10-11 0.338759354530751 1104 12-13 0.3181610512851165 1104 14-15 0.3493561201689559 1104 16-17 0.3810920733987828 1104 18-19 0.46631220243990157 1104 20-21 0.38505110253500163 1104 22-23 0.4713693023674068 1104 24-25 0.4058771100185865 1104 26-27 0.4369523809169067 1104 28-29 0.4902354347561513 1104 30-31 0.5907432868696887 1104 32-33 0.2666199111508334 1104 34-35 0.40240101641105497 1104 36-37 0.38542854724368425 1104 38-39 0.38868881036743375 1104 40-41 0.27670724292356397 1104 42-43 0.26479011452019563 1104 44-45 0.19250230385534195 1104 46-47 0.5106162914840056 1104 48-49 0.5231568686066268 1104 50-51 0.540515917795112 1104 52-53 0.48489390706351543 1104 54-55 0.5807230266035006 1104 56-57 0.7571689568630902 1104 58-59 0.6084679613058412 1104 60-61 0.4257284862724333 1104 62-63 0.61861393423316 1104 64-65 0.7057402764491556 1104 66-67 0.6089529265639797 1104 68-69 0.4856768641197604 1104 70-71 0.39570797789136947 1104 72-73 0.2724222463604704 1104 74-75 0.34908785574996415 1104 76-77 0.5266343971993734 1104 78-79 0.458773642177043 1104 80-81 0.5286852762409282 1104 82-83 0.47189138307113154 1104 84-85 0.49170684527712893 1104 86-87 0.6330267090249393 1104 88-89 0.5215390160827909 1104 90-91 0.4949016086054705 1104 92-93 0.41682673253673386 1104 94-95 0.6423461472542336 1104 96-97 0.5965609278332948 1104 98-99 0.33561409317468005 1104 100-101 0.5084600594340003 1105 1 0.2968509410350002 1105 2 0.37930469138140666 1105 3 0.2555263439849895 1105 4 0.1876891110821859 1105 5 -0.09393932881573619 1105 6 0.1812335197980417 1105 7 0.36856258653092766 1105 8 0.31245828084682614 1105 9 0.3076169224768037 1105 10-11 0.2517246947104752 1105 12-13 0.12489083305661808 1105 14-15 0.16187858486728857 1105 16-17 0.24883918508042058 1105 18-19 0.2764961990167123 1105 20-21 0.154629622004407 1105 22-23 0.11158967036056566 1105 24-25 0.20382961322782478 1105 26-27 0.24102490980008184 1105 28-29 0.14820612367356745 1105 30-31 0.2569745662877452 1105 32-33 0.3644686488487352 1105 34-35 0.2208883933900907 1105 36-37 0.10395229563906128 1105 38-39 0.20618239188220144 1105 40-41 0.14434417060862614 1105 42-43 0.22435793651420255 1105 44-45 0.18441180321350004 1105 46-47 0.26365972323411313 1105 48-49 0.36746902093867817 1105 50-51 0.19697933242925814 1105 52-53 0.11082886641310807 1105 54-55 0.26539354436131646 1105 56-57 0.43762680881003035 1105 58-59 0.3902876018706749 1105 60-61 0.3233846694132083 1105 62-63 0.32591603093833754 1105 64-65 0.3602492623284874 1105 66-67 0.3527719252802797 1105 68-69 0.13025859283178676 1105 70-71 0.1776249654823019 1105 72-73 0.033689897194875584 1105 74-75 0.22528918223691363 1105 76-77 0.1967317442254739 1105 78-79 0.2010725296395961 1105 80-81 0.2266666626337397 1105 82-83 0.021324416875152963 1105 84-85 0.3019721426669477 1105 86-87 0.15542294351359942 1105 88-89 0.11310191723811869 1105 90-91 0.18345851917457523 1105 92-93 0.02575056651191332 1105 94-95 0.07461615153322043 1105 96-97 0.35582494152606614 1105 98-99 -0.031238709563872646 1105 100-101 -0.023677296150076188 1106 1 0.026698310263405745 1106 2 0.029208615942003746 1106 3 0.23017534568850806 1106 4 0.39896223175778545 1106 5 0.19236548060772662 1106 6 0.16515501243898711 1106 7 0.16616837043385146 1106 8 0.21158209706016606 1106 9 0.23247220380022782 1106 10-11 0.36989143000245406 1106 12-13 0.3780319549593969 1106 14-15 0.34105423337650365 1106 16-17 0.5013006136172535 1106 18-19 0.5280301766206392 1106 20-21 0.35320403202855033 1106 22-23 0.38375261914993075 1106 24-25 0.2597400693035894 1106 26-27 0.15763758449188003 1106 28-29 0.2733933294929969 1106 30-31 0.29567873870682604 1106 32-33 0.3859942904954181 1106 34-35 0.3405638763911938 1106 36-37 0.3822905134800223 1106 38-39 0.41388245485601516 1106 40-41 0.4917320691400491 1106 42-43 0.4051674730107564 1106 44-45 0.397894558075798 1106 46-47 0.5563461822486531 1106 48-49 0.542193611407022 1106 50-51 0.36457748681198865 1106 52-53 0.34428814738128466 1106 54-55 0.46647277834134115 1106 56-57 0.5839117572603172 1106 58-59 0.6174550516732751 1106 60-61 0.508012498189018 1106 62-63 0.5493388597545135 1106 64-65 0.6872695713846042 1106 66-67 0.5334613674775852 1106 68-69 0.4570770627294891 1106 70-71 0.4158072827573065 1106 72-73 0.4186188699950293 1106 74-75 0.3379855717380451 1106 76-77 0.42274164645458256 1106 78-79 0.5144737414819076 1106 80-81 0.3631639256947494 1106 82-83 0.40798870183974856 1106 84-85 0.4430375304807086 1106 86-87 0.3720932432851143 1106 88-89 0.3851139912565813 1106 90-91 0.39402772578521805 1106 92-93 0.32569465706502854 1106 94-95 0.14481883841609644 1106 96-97 0.28092041144799396 1106 98-99 0.0664383235619681 1106 100-101 0.04219392239328812 1107 1 0.15733210673490916 1107 2 0.0405959532911595 1107 3 0.09285483188304511 1107 4 0.17188320803748525 1107 5 0.2581001801180349 1107 6 0.049509895971546314 1107 7 0.07459259095704596 1107 8 0.25333756428149456 1107 9 0.23846464069610818 1107 10-11 0.2220172844558519 1107 12-13 0.06903874695696288 1107 14-15 0.11441719692962948 1107 16-17 0.03860727975277456 1107 18-19 0.1730356730074405 1107 20-21 -0.020892194102003714 1107 22-23 0.08674807882143654 1107 24-25 0.2125508143366801 1107 26-27 0.0910653854913761 1107 28-29 0.2555729906803421 1107 30-31 0.22537003999892846 1107 32-33 0.1646890812106534 1107 34-35 0.157906470590909 1107 36-37 0.2322922681505375 1107 38-39 0.30467081408733776 1107 40-41 0.39913573535808666 1107 42-43 0.3336655983587278 1107 44-45 0.20505294981121125 1107 46-47 0.4715548125718243 1107 48-49 0.30294523637418536 1107 50-51 0.07769834377409524 1107 52-53 0.21613554985243155 1107 54-55 0.37995139839257774 1107 56-57 0.45872075900454945 1107 58-59 0.26982874147977753 1107 60-61 0.3935634030773443 1107 62-63 0.4696047350173558 1107 64-65 0.4178048217666017 1107 66-67 0.20207714111180763 1107 68-69 0.263151352633308 1107 70-71 0.35347000473498724 1107 72-73 0.452600198403907 1107 74-75 0.40190442439219964 1107 76-77 0.362663151032379 1107 78-79 0.3656207743340829 1107 80-81 0.4188315588529008 1107 82-83 0.42292189631699273 1107 84-85 0.45166813819424334 1107 86-87 0.37797518336264346 1107 88-89 0.3591140424739834 1107 90-91 0.371998074699075 1107 92-93 0.33384628715393205 1107 94-95 0.3921581414230353 1107 96-97 0.49960973897289307 1107 98-99 0.5828648849104638 1107 100-101 0.6173440889921409 1108 1 0.24096238993985608 1108 2 0.2502655610467599 1108 3 0.08191571269453846 1108 4 -0.02188503848367418 1108 5 0.26608738759873773 1108 6 0.4133093391398006 1108 7 0.16641030296842985 1108 8 0.366665540341522 1108 9 0.2805895842147521 1108 10-11 0.1592478988376982 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-1.6090116523653002 2316 96-97 -1.4220701755275158 2316 98-99 -1.0257349873579784 2316 100-101 -1.6208709488094968 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 11.0 7 211.0 8 602.0 9 556.0 10 463.0 11 525.0 12 709.0 13 968.0 14 1277.0 15 1580.0 16 2145.0 17 2771.0 18 3426.0 19 4157.0 20 5082.0 21 5963.0 22 6863.0 23 7287.0 24 7933.0 25 8563.0 26 9645.0 27 11026.0 28 12688.0 29 15132.0 30 18464.0 31 22268.0 32 27970.0 33 36550.0 34 51001.0 35 77686.0 36 134210.0 37 240267.0 38 278574.0 39 72487.0 40 82.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.161154007707296 17.24452563318289 12.207440985465901 24.386879373643918 2 15.898754424847294 18.74614541942061 40.29659501488326 25.058505140848837 3 20.241463362062905 22.69648769488987 28.446494489151203 28.61555445389602 4 12.654765386547878 15.013742341410005 36.7118307873443 35.619661484697815 5 14.056317532469967 37.22916690107302 34.413946615359805 14.300568951097208 6 34.287883118647876 35.346768940973604 16.62700960110209 13.738338339276426 7 30.167354332199146 30.94045094491148 20.1662017154311 18.725993007458275 8 26.7815793591965 35.93454961864966 18.522877740179492 18.760993281974343 9 26.180641195278557 15.899438869793054 18.98781031774888 38.932109617179506 10-11 26.01042169436882 25.539383601763817 27.48112708000431 20.969067623863054 12-13 26.39548956519172 22.538539281462207 27.69332191949307 23.372649233853 14-15 23.09953070782755 24.560451532638417 24.994177778090172 27.345839981443866 16-17 23.410489579881336 27.61570160509707 25.602824132062434 23.370984682959158 18-19 23.304675391144187 27.15466727422689 26.836165989321227 22.704491345307698 20-21 24.049853269574776 26.16155545942407 26.83803533221873 22.95055593878243 22-23 23.697740805243832 26.024232515418905 26.669750136090435 23.608276543246827 24-25 23.63114534832604 26.015814550359075 26.8401671620795 23.51287293923539 26-27 23.622820916211317 26.17243546694452 26.850642851931738 23.354100764912424 28-29 23.38973681699905 26.540814971257326 26.74934667237841 23.320101539365208 30-31 23.6954024816161 26.280512785018267 26.547689642722855 23.476395090642775 32-33 23.543411445813557 26.428388371236 26.6648396564722 23.363360526478242 34-35 23.840752678315884 26.310957758651334 26.676250675775528 23.17203888725726 36-37 23.721778772136908 26.200027685751753 26.705199122286842 23.372994419824494 38-39 23.641106606980177 26.462200530893 26.7209126570652 23.17578020506163 40-41 23.713734938857513 26.46430502215795 26.604510906876726 23.217449132107802 42-43 23.495008146719517 26.384381120562097 26.53141491027385 23.589195822444537 44-45 23.65648094989684 26.39207969752851 26.452253691686252 23.499185660888404 46-47 23.848610065857976 26.40190704156447 26.37158819223744 23.377894700340114 48-49 23.65864126947277 26.34072685862779 26.566281724064815 23.43435014783462 50-51 23.390617286261445 26.69798761356378 26.548433211746293 23.362961888428487 52-53 23.69238660065676 26.68978791689266 26.191905752904628 23.425919729545953 54-55 23.467999458970443 26.5100270655184 26.429340650648886 23.592632824862275 56-57 23.64017706033956 26.546270005313684 26.310969829049224 23.502583105297532 58-59 23.837539406336376 26.444220286978766 26.28629069062603 23.431949616058827 60-61 23.6486071989679 26.549735535216314 26.56219195530674 23.23946531050905 62-63 23.438533463897933 26.616615861161513 26.603209395401205 23.341641279539342 64-65 23.50778399877505 26.691902365565824 26.41006106483474 23.39025257082439 66-67 23.551477031855054 26.61227352053389 26.221294500078645 23.614954947532414 68-69 23.44653530980345 26.51334416946309 26.35150444930489 23.688616071428573 70-71 23.59178117968969 26.33845554738543 26.384277918531662 23.685485354393215 72-73 23.388635816953336 26.366003479882107 26.575470294180136 23.669890408984422 74-75 23.24839526477253 26.722453274540264 26.366173253804092 23.662978206883118 76-77 23.307954961904326 26.490951984171247 26.34239337756662 23.858699676357812 78-79 23.420793477085088 26.43888724211386 26.393511175670938 23.746808105130118 80-81 23.46584404436619 26.43596173701735 26.23372283394693 23.86447138466953 82-83 23.504860971670112 26.322357006085166 26.577259049467187 23.59552297277753 84-85 23.46743173957188 26.475155025097518 26.424786789291787 23.632626446038817 86-87 23.290904181312673 26.55660264777002 26.435675118646095 23.716818052271204 88-89 23.322857482556632 26.38786299197413 26.277019316195627 24.01226020927361 90-91 23.353533669984802 26.258346450131043 26.406009912272733 23.98210996761142 92-93 23.455710491678882 26.314024347402494 26.489486774742026 23.740778386176594 94-95 23.407608789811242 26.314929371434566 26.45966560358193 23.81779623517226 96-97 23.21022279159047 26.201871207841304 26.68790408152471 23.90000191904351 98-99 23.35142167840186 26.19697809976436 26.400934135491767 24.050666086342012 100-101 23.320309027014126 26.09298886772764 26.376185640819266 24.210516464438964 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 22.0 1 21.0 2 26.5 3 45.5 4 48.0 5 45.5 6 64.0 7 132.5 8 207.5 9 235.0 10 248.0 11 246.0 12 241.0 13 250.0 14 298.5 15 352.5 16 362.5 17 396.0 18 461.0 19 528.0 20 617.0 21 716.0 22 856.0 23 1035.0 24 1274.0 25 1626.0 26 2050.0 27 2714.5 28 3676.0 29 4938.5 30 6571.0 31 8503.0 32 11056.0 33 13896.5 34 16528.5 35 19056.5 36 21677.0 37 23977.5 38 25706.5 39 27653.0 40 29830.0 41 31694.5 42 33235.5 43 35056.0 44 37347.5 45 39464.5 46 41461.5 47 44329.0 48 47971.5 49 48236.5 50 46050.0 51 45275.0 52 44661.0 53 43582.5 54 42003.0 55 39751.0 56 36928.0 57 33521.0 58 30114.0 59 26531.5 60 22240.5 61 18089.0 62 14300.5 63 10711.0 64 7614.0 65 5449.0 66 4070.5 67 2987.0 68 2192.5 69 1631.0 70 1198.5 71 876.0 72 585.5 73 427.5 74 349.0 75 277.0 76 198.5 77 126.5 78 92.0 79 70.5 80 57.5 81 44.0 82 29.0 83 21.5 84 19.0 85 15.0 86 12.5 87 10.0 88 9.0 89 10.5 90 8.5 91 6.0 92 4.5 93 3.5 94 3.0 95 1.5 96 1.5 97 3.0 98 2.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4634557430163626 2 0.3601953716157442 3 0.1931455316506133 4 0.22129894812849932 5 0.24486925029603177 6 0.19426792699192438 7 0.18753355494405796 8 0.188281818504932 9 0.18800121966960423 10-11 0.19987990369847972 12-13 0.18860918381281438 14-15 0.19730774770797518 16-17 0.08605030950051537 18-19 0.03615048328472738 20-21 4.208982529916513E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.13141378787850444 46-47 0.2010022990397908 48-49 0.08301048878446454 50-51 0.05934665367182283 52-53 0.0670163551707818 54-55 0.07562138612083334 56-57 0.10704845567754331 58-59 0.08834186665569214 60-61 0.13277001558258866 62-63 0.23313086568482017 64-65 0.12556797880917597 66-67 0.09858372414515565 68-69 0.10382156907127398 70-71 0.0829169558393553 72-73 0.06687605575311792 74-75 0.13492127332010154 76-77 0.20792373697787575 78-79 0.13080582373529429 80-81 0.07959653628797672 82-83 0.0831040217295738 84-85 0.09432797514268451 86-87 0.06860641523763916 88-89 0.09082048970108741 90-91 0.1097141446131571 92-93 0.15685474894822204 94-95 0.12510031408362968 96-97 0.08431995001599413 98-99 0.07244126598711864 100-101 0.05864515658350341 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1069142.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.2684859652639 #Duplication Level Percentage of deduplicated Percentage of total 1 79.5980732474804 55.93236092852625 2 12.856501735471081 18.06813823522681 3 3.8322152502283755 8.078518905796287 4 1.5447758191966603 4.341962318827982 5 0.7663339469761478 2.692456309889937 6 0.43030334904086764 1.8142058905730558 7 0.25920406391629497 1.2749713989209077 8 0.16721491458716226 0.939995110308065 9 0.11140590276276612 0.7045491703259688 >10 0.42086667390669696 4.616427878699649 >50 0.00883077261227507 0.39724351235452643 >100 0.003205745330329853 0.47783812940645476 >500 9.350061795680816E-4 0.47905756538162997 >1k 1.335723113668688E-4 0.18227464576236782 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1942 0.18164097940217483 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 9.353294510925583E-5 0.0 0.0 0.0 0.0 9 9.353294510925583E-5 0.0 0.0 0.0 0.0 10-11 9.353294510925583E-5 0.0 0.0 1.8706589021851166E-4 0.0 12-13 9.353294510925583E-5 0.0 0.0 1.8706589021851166E-4 0.0 14-15 9.353294510925583E-5 0.0 0.0 3.273653078823954E-4 0.0 16-17 9.353294510925583E-5 0.0 0.0 7.482635608740466E-4 0.0 18-19 9.353294510925583E-5 0.0 0.0 0.0010756288687564421 0.0 20-21 9.353294510925583E-5 0.0 0.0 0.0019174253747397446 0.0 22-23 9.353294510925583E-5 0.0 0.0 0.003507485441597094 1.8706589021851166E-4 24-25 9.353294510925583E-5 0.0 0.0 0.004770180200572047 3.741317804370233E-4 26-27 9.353294510925583E-5 0.0 0.0 0.00561197670655535 4.2089825299165124E-4 28-29 9.353294510925583E-5 0.0 0.0 0.006547306157647908 4.6766472554627916E-4 30-31 9.353294510925583E-5 0.0 0.0 0.00874533036771542 5.144311981009071E-4 32-33 9.353294510925583E-5 0.0 0.0 0.014918504744926306 8.417965059833025E-4 34-35 9.353294510925583E-5 0.0 0.0 0.023476769222423213 9.353294510925583E-4 36-37 9.353294510925583E-5 4.6766472554627914E-5 0.0 0.03905000458311431 9.353294510925583E-4 38-39 9.353294510925583E-5 9.353294510925583E-5 0.0 0.06425713329005875 9.353294510925583E-4 40-41 9.353294510925583E-5 9.353294510925583E-5 0.0 0.09484240634078542 9.353294510925583E-4 42-43 9.353294510925583E-5 9.353294510925583E-5 0.0 0.12425851757764637 9.353294510925583E-4 44-45 9.353294510925583E-5 9.353294510925583E-5 0.0 0.15989456966427285 9.353294510925583E-4 46-47 9.353294510925583E-5 9.353294510925583E-5 0.0 0.2064272098561276 9.353294510925583E-4 48-49 9.353294510925583E-5 9.353294510925583E-5 0.0 0.25267925121265467 0.0011223953413110699 50-51 9.353294510925583E-5 9.353294510925583E-5 0.0 0.3032338080442074 0.0011223953413110699 52-53 9.353294510925583E-5 9.353294510925583E-5 0.0 0.35135650830291953 0.0012159282864203256 54-55 9.353294510925583E-5 9.353294510925583E-5 0.0 0.40649417944482585 0.0013094612315295816 56-57 9.353294510925583E-5 9.353294510925583E-5 0.0 0.4746796964294734 0.0013094612315295816 58-59 9.353294510925583E-5 9.353294510925583E-5 0.0 0.5539956338821224 0.0013094612315295816 60-61 9.353294510925583E-5 9.353294510925583E-5 0.0 0.6361175596880488 0.0014029941766388375 62-63 9.353294510925583E-5 9.353294510925583E-5 0.0 0.7275460135323465 0.0014029941766388375 64-65 9.353294510925583E-5 9.353294510925583E-5 0.0 0.8157008142978202 0.0014029941766388375 66-67 9.353294510925583E-5 9.353294510925583E-5 0.0 0.9191482515886571 0.0014029941766388375 68-69 9.353294510925583E-5 9.353294510925583E-5 0.0 1.0428923379682025 0.0014029941766388375 70-71 9.353294510925583E-5 9.353294510925583E-5 0.0 1.181321096729901 0.0014029941766388375 72-73 9.353294510925583E-5 9.353294510925583E-5 0.0 1.324379736274508 0.0018706589021851166 74-75 9.353294510925583E-5 9.353294510925583E-5 0.0 1.4605637043535844 0.0018706589021851166 76-77 9.353294510925583E-5 9.353294510925583E-5 0.0 1.6183070162803443 0.0018706589021851166 78-79 9.353294510925583E-5 9.353294510925583E-5 0.0 1.8092077572483356 0.0018706589021851166 80-81 9.353294510925583E-5 9.353294510925583E-5 0.0 2.020077781997153 0.0019174253747397444 82-83 9.353294510925583E-5 9.353294510925583E-5 0.0 2.2430135566650637 0.0019641918472943724 84-85 9.353294510925583E-5 9.353294510925583E-5 0.0 2.4629095106169245 0.0021044912649582563 86-87 9.353294510925583E-5 9.353294510925583E-5 0.0 2.700015526468888 0.0021512577375128843 88-89 9.353294510925583E-5 9.353294510925583E-5 0.0 2.96751974948136 0.002198024210067512 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATAC 35 2.779286E-4 54.31955 3 CATACCG 100 4.9317874E-5 28.517765 5 GACCGTA 100 4.941253E-5 28.509745 7 GTACATG 3280 0.0 27.30916 1 TACATGG 3255 0.0 26.899265 2 ACATGGG 3430 0.0 24.2498 3 CATGGGG 2235 0.0 22.542007 4 CCGTATA 130 2.9317362E-4 21.933657 9 GTATTAT 320 1.6370905E-11 20.845024 1 GTATCCG 115 0.0036575873 20.715551 1 GAGTACA 2490 0.0 20.282938 1 CCTACAC 540 0.0 20.244093 3 GGACCGT 215 1.0746298E-6 19.891453 6 ACCGTAT 145 6.125704E-4 19.664658 8 AGTACAT 2470 0.0 19.45799 2 GTGTATA 695 0.0 19.195417 1 TATGACG 125 0.0060091997 19.011843 4 TATACCC 275 2.8165232E-8 19.011843 5 ATTATAC 445 0.0 18.15738 3 AGAGTAC 2850 0.0 17.841103 10-11 >>END_MODULE