FastQCFastQC Report
Fri 10 Feb 2017
SRR2031626_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031626_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069142
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22050.2062401439659091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG30650.029.9202651
TACATGG30200.028.4673922
ACATGGG31950.025.7188363
GACCGTG2900.024.5668247
TCTATAC3100.022.9829443
CATGGGG20000.022.0866094
CTATACT4100.022.0113074
TAATACG1353.7967414E-421.1102584
GTCTAGG3850.020.981031
AGACCGT2509.496944E-920.898186
GTATAGA4950.020.1582431
GTATATA6450.019.890361
GTATAAT4300.019.890361
CGTACAC1759.939027E-518.9992313
ACCGTGT2502.1736741E-718.9983448
ACGCGCA650.007562881318.27020380-81
TACACGA1300.007564550718.2684942
CACCGTA1300.007566591718.267647
GAGTACA23950.018.2524381
GTATAGG3156.7757355E-918.101281
Produced by FastQC (version 0.11.5)