FastQCFastQC Report
Fri 10 Feb 2017
SRR2031625_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031625_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3149701
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC310890.9870460719922304No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC192240.610343648492349No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT183080.5812615229191597No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA171230.5436389041372498No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC167040.5303360541206927No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT164130.5210970819134896No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT123870.3932754251911531No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA117130.3718765686012736No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT116290.36920964878888507No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT116100.36860641692655904No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA107380.34092124935033513No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT104810.33276174468624165No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA97710.31021992246248137No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG94840.30110794643682054No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC93400.2965360839012973No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA93320.29628209153821267No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT93050.2954248673128021No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG90080.28599540083328545No Hit
CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA87540.2779311433053487No Hit
ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG86600.27494673303910433No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT85800.2724068094082581No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC85150.27034312145819556No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA83300.2644695480618636No Hit
AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT76530.24297544433582743No Hit
CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC75910.2410070035219216No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG73990.23491118680789067No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA73860.23449844921787813No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA72500.23018057904543957No Hit
ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA71050.22557696746453074No Hit
CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT69640.2211003520651643No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC65840.2090357148186447No Hit
ATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC63320.20103495538147909No Hit
AAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCA61870.19643134380057028No Hit
CTACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTC61820.19627259857364238No Hit
GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT61190.194272408714351No Hit
GGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCT59200.18795434868262098No Hit
GAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAA58640.18617640214102862No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA58410.18544617409716033No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT56040.17792165034077836No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC54990.1745880005752927No Hit
GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA52640.1671269749096819No Hit
GCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGT52620.16706347681891073No Hit
GTGATGTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAA52190.16569826786733088No Hit
TCAGAATACTTTGAAACATCATTCTCCATTTCAGATGGTATCTATGGTTC51830.16455530223345008No Hit
TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC51780.1643965570065222No Hit
ACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAG51690.164110815598052No Hit
ATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGC49850.15826899124710567No Hit
TCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAA49410.15687203325014026No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC47940.15220492357846033No Hit
ACTTAACCCTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCAT47790.15172868789767666No Hit
TAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCA47050.14937925853914388No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT46430.14741081772523806No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG45300.14382317559666774No Hit
ACATAAATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACC45000.14287070423510043No Hit
ACAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGT44900.14255321378124464No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA44610.14163249146506288No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC44360.14083876533042344No Hit
GTTTTAGGTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCA43650.1385845831080474No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG43580.13836233979034834No Hit
GTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTC42720.13563192188718864No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA42380.134552454344079No Hit
CTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTC42110.1336952301186684No Hit
GTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATT42100.13366348107328282No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT42080.13359998298251166No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC41850.1328697549386434No Hit
TTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACATCATTCTCCA41830.13280625684787223No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC41780.13264751162094435No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT41320.13118705553320775No Hit
ATCCAGTAGCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAG40550.12874237903851826No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG40110.12734542104155283No Hit
CAATAGATGCACAAGAAGTTGAAACCATTTGAACTATTCTACCAGCTGTA40020.12705967963308262No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG39780.12629770254382877No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG39110.12417051650299504No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC39060.12401177127606716No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT38160.12115435719136515No Hit
ATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGCTTCA38110.12099561196443727No Hit
GAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGA37790.11997964251209876No Hit
TGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCTATTGTGCTTG37760.11988439537594205No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA37570.11928116351361605No Hit
ATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAG37470.11896367305976029No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA37440.11886842592360354No Hit
TATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGCT37370.1186461826059045No Hit
GTAGTAGGCAAACAATAAGGAATGTTGATCCAATAATTACATGGAGTCCA37260.11829694310666315No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC36470.1157887685212025No Hit
CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG36110.1146458028873217No Hit
CTTCAGAATACTTTGAAACATCATTCTCCATTTCAGATGGTATCTATGGT36040.11442355956962265No Hit
GTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAG35990.11426481434269474No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG35910.11401082197961014No Hit
TCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACA35820.11372508057113993No Hit
GATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGC35600.11302660157265722No Hit
CGCTAATATTAACAACAAAACTAACACATACAAGCACAATAGATGCACAA35540.11283610730034375No Hit
TACAAGCACAATAGATGCACAAGAAGTTGAAACCATTTGAACTATTCTAC35050.11128040407645044No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC34980.1110581607587514No Hit
TATTAAACCCGAATGATATTTCCTATTTGCATACGCCATTCTACGCTCAA34970.11102641171336582No Hit
CACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCG34720.11023268557872636No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC34350.10905797089945997No Hit
ATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCTT34230.10867698235483304No Hit
ATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAAC33670.10689903581324069No Hit
AATCCGAAGTGATGTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAG33320.10578781922474546No Hit
GTGAAGTAAAGTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTG33270.10562907399781757No Hit
ATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAA32600.10350188795698385No Hit
ATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTC32360.10273991086772999No Hit
GTTTCAAAGTATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTAT32150.10207318091463286No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT32150.10207318091463286No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC31960.10146994905230687No Hit
GTCGTAGTAGGCAAACAATAAGGAATGTTGATCCAATAATTACATGGAGT31890.10124770573460783No Hit
CTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGCCTTATAGA31850.10112070955306551No Hit
GAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCTTGGAGGATGGTGAA31550.10016823819149817No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGGT4300.048.7457921
GGCGTAC405.382404E-447.5129058
GCATAGC751.3609133E-744.3453759
TAACTTT21600.043.9899256
ACTTTAT23500.040.638698
CTCGAGT1305.456968E-1240.2032289
AACTTTA25300.038.306537
CTCCGTA250.001610807738.02665392-93
ACGTGGG756.902659E-638.0187873
GCGTACA500.00161408838.010329
TACGTGG651.3143441E-436.6060372
TCTCGAG1451.6370905E-1136.044278
CGCAGAA4100.034.7655379
CATGGGT9050.034.1350634
AACTTGC2150.033.1448447
ACTTAAT93550.033.0995831
CTTAATA93650.033.0294652
ATCTATA29050.032.961191
CTTACAC5650.032.803823
ATAACTT29750.032.749365
Produced by FastQC (version 0.11.5)