FastQCFastQC Report
Fri 10 Feb 2017
SRR2031625_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031625_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3149701
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC340771.0819122196043371No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT191820.6090101885861547No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC184840.5868493549070214No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA177350.5630693199132235No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC170910.5426229346849114No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT156670.49741229405584847No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT125050.39702181254665125No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT121540.3858778976163134No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT120950.38400470393856434No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT119880.3806075560823075No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA119630.37981382994766805No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC108910.34577885329432856No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG105120.33374596509319454No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT103620.3289836082853579No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA102680.32599919801911353No Hit
ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG97670.31009292628093904No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG95740.30396536052152257No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA93450.2966948291282252No Hit
CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA93040.2953931182674165No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT92520.29374216790736646No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA90760.28815433591950473No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT88320.2804075688454237No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC86730.27535947062911686No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA80130.25440510067463545No Hit
AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT79770.2532621350407547No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA78490.24919825723140068No Hit
ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA77720.2467535807367112No Hit
CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC73520.23341898167476852No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG71390.2266564350076404No Hit
CTACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTC69300.22002088452205462No Hit
CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT68770.21833818511661898No Hit
ATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC68750.21827468702584782No Hit
AAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCA65070.2065910383239552No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC64560.20497183700929072No Hit
GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT63770.20246366242383007No Hit
GAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAA62780.19932050693065786No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA61910.19655833998211258No Hit
TCAGAATACTTTGAAACATCATTCTCCATTTCAGATGGTATCTATGGTTC58680.18630339832257095No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT58510.18576366455101612No Hit
TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC57640.18300149760247084No Hit
ACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAG56990.18093780965240827No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA56570.179604349746214No Hit
ACATAAATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACC54050.17160359030904837No Hit
GGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCT53160.16877792526973195No Hit
GTGATGTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAA52510.1667142373196694No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC51000.16192013146644713No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC50670.16087241296872307No Hit
TCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAA50480.16026918110639707No Hit
ATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGC50000.15874522692788934No Hit
GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA49760.1579832498386355No Hit
GCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGT49220.15626880138781427No Hit
ACAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGT49180.15614180520627197No Hit
TAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCA48280.15328439112156997No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC47400.1504904751276391No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT46980.14915701522144484No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG46190.14664884063598418No Hit
ACTTAACCCTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCAT45480.14439465841360816No Hit
TTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACATCATTCTCCA45010.14290245328048598No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT44780.1421722252366177RNA PCR Primer, Index 39 (95% over 23bp)
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC44020.13975929778731377No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA43950.13953705446961473No Hit
CAATAGATGCACAAGAAGTTGAAACCATTTGAACTATTCTACCAGCTGTA43550.13826709265419163No Hit
GTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTC43450.13794960220033584No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG43340.1376003627010945No Hit
CTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTC43290.13744161747416658No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC43250.13731462129262428No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA42960.13639389897644252No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC42800.13588591425027327No Hit
ATCCAGTAGCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAG42210.1340127205725242No Hit
GTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATT40860.12972659944547116No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG40760.1294091089916154No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT40040.1271231777238538No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC39090.1241070184122239No Hit
GTTTTAGGTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCA39000.12382127700375369No Hit
TATTAAACCCGAATGATATTTCCTATTTGCATACGCCATTCTACGCTCAA38680.1228053075514152No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG38660.12274180946064404No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG38060.12083686673750937No Hit
ATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGCTTCA38020.12070987055596706No Hit
GAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGA37990.12061462341981033No Hit
TATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGCT37830.12010663869364109No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT37560.11924941446823048No Hit
CTTCAGAATACTTTGAAACATCATTCTCCATTTCAGATGGTATCTATGGT37100.1177889583804939No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT37040.11759846410818044No Hit
TGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCTATTGTGCTTG36930.11724922460893906No Hit
CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG36810.11686823606431214No Hit
ATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAAC36790.11680473797354098No Hit
ATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAG36710.11655074561045635No Hit
TACAAGCACAATAGATGCACAAGAAGTTGAAACCATTTGAACTATTCTAC36630.11629675324737172No Hit
CACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCG36400.11556652520350345No Hit
GATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGC36320.11531253284041883No Hit
ATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCTT36310.11528078379503325No Hit
CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC36220.11499504238656304No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG36120.11467755193270728No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC35250.111915384984162No Hit
GTAGTAGGCAAACAATAAGGAATGTTGATCCAATAATTACATGGAGTCCA35140.11156614548492062No Hit
CGCTAATATTAACAACAAAACTAACACATACAAGCACAATAGATGCACAA34940.11093116457720907No Hit
ATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTC34480.1094707084894725No Hit
ATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAA34290.1088674766271465No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA33720.10705778104016857No Hit
GTTTCAAAGTATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTAT33670.10689903581324069No Hit
TAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATC33560.10654979631399931No Hit
GTGAAGTAAAGTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTG33470.10626405490552913No Hit
AATCCGAAGTGATGTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAG33400.10604181158783009No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA33350.10588306636090218No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC33270.10562907399781757No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC33020.10483534786317812No Hit
GTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAG32920.10451785740932235No Hit
ATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTATAATGGTAATT32660.10369238222929732No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT32470.10308915036697135No Hit
GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCT32430.10296215418542903No Hit
GAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCTTGGAGGATGGTGAA31960.10146994905230687No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT25350.049.2754446
CGCAGAA2700.047.4955339
CGTACAC405.392118E-447.495538
ACTTTAT26550.047.04838
AACTTTA28700.044.1857387
GTATGGT4750.041.017631
AACTTGC2150.039.7637027
ATAACTT33600.038.7314765
GGAGTGA2600.038.361786
ATCTATA32350.038.3395081
GGTGGAC756.9305916E-637.9964268
CATTCCG4250.035.761349
ACTTAAT93250.035.4173661
CTTAATA93300.035.3826522
TAGACCC6500.035.0736245
CATGGGT7650.034.770734
ACTTCAG96950.034.047858
GTGGACA1351.1996235E-831.6636879
TACGTAC600.00395380831.6636876
TCTAGAC7700.031.4605773
Produced by FastQC (version 0.11.5)