FastQCFastQC Report
Fri 10 Feb 2017
SRR2031624_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031624_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1447342
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25010.1727995180130197No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21520.14868635056538124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21750.040.954871
GTATCAA34300.037.0800551
TCAACGC39850.031.2638914
AACGCAG40650.030.868666
CAACGCA40400.030.6028615
ATCAACG40700.030.4941253
TATACCG1102.7108108E-630.2604485
TATCAAC43300.028.7958372
TACCGTG2600.027.4196727
ACGCAGA47550.026.2875187
CGCAGAG47400.025.869368
GTACATG47950.024.7362521
TACATGG47900.023.3479462
ACATGGG45600.022.9418223
GCAGAGT56000.021.7268269
TATACTG8050.020.6748435
GTATACC2851.9390427E-920.0219544
ACCGTGC3404.1836756E-1119.5701188
ATACCGT2451.7448656E-719.4002976
GAGTACT30000.019.1680712-13
Produced by FastQC (version 0.11.5)