##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031624_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1447342 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.923200598061825 33.0 31.0 34.0 30.0 34.0 2 32.16418026976347 34.0 31.0 34.0 30.0 34.0 3 32.31627700985669 34.0 31.0 34.0 30.0 34.0 4 35.394243378551856 37.0 35.0 37.0 33.0 37.0 5 35.09875896643641 37.0 35.0 37.0 32.0 37.0 6 35.31069850802368 37.0 35.0 37.0 32.0 37.0 7 35.464112835805224 37.0 35.0 37.0 33.0 37.0 8 35.54418306108715 37.0 35.0 37.0 33.0 37.0 9 37.475700283692454 39.0 38.0 39.0 35.0 39.0 10-11 37.42960198764355 39.0 38.0 39.0 35.0 39.0 12-13 37.44701286910765 39.0 38.0 39.0 35.0 39.0 14-15 38.87028601394833 41.0 39.0 41.0 35.5 41.0 16-17 38.822819002005055 41.0 39.0 41.0 35.0 41.0 18-19 38.74271941255073 41.0 39.0 41.0 34.0 41.0 20-21 38.7305978821868 41.0 39.0 41.0 34.0 41.0 22-23 38.66753365825078 40.5 39.0 41.0 34.0 41.0 24-25 38.58574027424064 40.5 38.0 41.0 34.0 41.0 26-27 38.485127910334946 40.0 38.0 41.0 34.0 41.0 28-29 38.36761801979076 40.0 38.0 41.0 34.0 41.0 30-31 38.22416471020671 40.0 38.0 41.0 34.0 41.0 32-33 38.056226517298605 40.0 38.0 41.0 33.0 41.0 34-35 37.91529023547993 40.0 38.0 41.0 33.0 41.0 36-37 37.782481265658014 40.0 38.0 41.0 33.0 41.0 38-39 37.598360304613564 40.0 37.5 41.0 32.0 41.0 40-41 37.44207519715451 40.0 37.0 41.0 31.0 41.0 42-43 37.24515629339852 40.0 37.0 41.0 31.0 41.0 44-45 37.0245253022437 40.0 36.5 41.0 30.5 41.0 46-47 36.82317897221251 40.0 36.5 41.0 30.0 41.0 48-49 36.94766544465648 40.0 37.0 41.0 30.0 41.0 50-51 36.70465860867715 39.5 36.5 40.5 30.0 41.0 52-53 36.81225584554308 39.5 36.0 40.5 30.5 41.0 54-55 37.06523199078034 40.0 36.0 41.0 31.0 41.0 56-57 36.94760464354658 40.0 36.0 41.0 30.5 41.0 58-59 36.73424905792825 39.5 35.5 41.0 30.0 41.0 60-61 36.44318550833182 39.0 35.0 41.0 29.0 41.0 62-63 36.10534068658272 39.0 35.0 41.0 28.5 41.0 64-65 35.824302065441344 38.5 35.0 41.0 28.0 41.0 66-67 35.59493126019973 37.5 35.0 40.5 28.0 41.0 68-69 35.333541761380516 37.0 35.0 40.0 28.0 41.0 70-71 35.029313044187205 36.5 34.5 39.5 28.0 41.0 72-73 34.67414059703927 36.0 34.0 39.0 27.5 41.0 74-75 34.26191667207888 35.5 34.0 39.0 26.0 41.0 76-77 33.87443810792473 35.0 34.0 37.5 26.0 40.0 78-79 33.53681679934667 35.0 34.0 37.0 26.0 39.0 80-81 33.218541989384676 35.0 33.5 37.0 26.0 39.0 82-83 32.90675147960883 35.0 33.0 36.0 25.0 38.5 84-85 32.650631295160366 35.0 33.0 36.0 25.0 37.0 86-87 32.414356454797826 35.0 33.0 35.5 25.0 37.0 88-89 32.175657861099864 35.0 33.0 35.0 24.5 36.5 90-91 31.98771713941833 35.0 33.0 35.0 24.0 36.0 92-93 31.814525523338645 35.0 32.0 35.0 24.0 36.0 94-95 31.659726933924393 35.0 32.0 35.0 23.0 36.0 96-97 31.555687252909124 35.0 32.0 35.0 23.0 35.0 98-99 31.439889811806747 35.0 32.0 35.0 21.5 35.0 100-101 30.982243657684222 34.5 31.0 35.0 19.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06018764701134316 1101 2 0.10561680540066476 1101 3 0.09538741370872827 1101 4 0.008920753909158918 1101 5 0.13118731873316847 1101 6 0.13971166731658258 1101 7 0.06391296130721713 1101 8 0.283852434390262 1101 9 0.09480029794984546 1101 10-11 0.3223074923031959 1101 12-13 0.2623727815558965 1101 14-15 0.275214400042735 1101 16-17 0.1372682622704815 1101 18-19 0.20315045686870548 1101 20-21 0.03485062957128093 1101 22-23 0.12470874792024489 1101 24-25 0.20890370289574633 1101 26-27 0.02759669260684916 1101 28-29 0.1887192434410636 1101 30-31 0.2549133140216995 1101 32-33 0.24125780025214283 1101 34-35 0.16385183725203234 1101 36-37 0.2362633458812482 1101 38-39 0.24425182233099463 1101 40-41 0.0883589005751304 1101 42-43 0.20835937589795606 1101 44-45 0.06074790334992741 1101 46-47 0.03565431475817604 1101 48-49 0.08461797593572129 1101 50-51 0.17603119788176969 1101 52-53 0.19169110053827154 1101 54-55 0.05197972175393062 1101 56-57 0.2814056895405699 1101 58-59 0.3443649279574501 1101 60-61 0.11645303464914036 1101 62-63 -0.03421781890158826 1101 64-65 0.07028711963383216 1101 66-67 -0.02555123833322881 1101 68-69 -0.039561867462722944 1101 70-71 0.08388478361977292 1101 72-73 -0.043962030968422994 1101 74-75 -0.15497763294450095 1101 76-77 -0.15902511365266747 1101 78-79 -0.006702894270546267 1101 80-81 -0.0863194669054792 1101 82-83 0.01910636755258821 1101 84-85 -0.21183581489695058 1101 86-87 -0.14747605956507215 1101 88-89 0.012206291295484561 1101 90-91 -0.07865680378767337 1101 92-93 -0.2445661620557722 1101 94-95 -0.29780071277606623 1101 96-97 -0.14023539674147045 1101 98-99 -0.20143802638793673 1101 100-101 0.011651004327898562 1102 1 0.056510702957449865 1102 2 0.04268324264833012 1102 3 0.0601600459333298 1102 4 0.20330954742807705 1102 5 0.25278669314153035 1102 6 0.14640637408378154 1102 7 0.03491030997764 1102 8 0.2204402515507482 1102 9 0.24377992771961488 1102 10-11 0.20616677666990313 1102 12-13 0.26805017170229917 1102 14-15 0.23498940213379882 1102 16-17 0.3006360350075923 1102 18-19 0.33896869806932983 1102 20-21 0.2989022116728748 1102 22-23 0.271163839282913 1102 24-25 0.4550369039945181 1102 26-27 0.33946879416723874 1102 28-29 0.3081963592936745 1102 30-31 0.48957058619257054 1102 32-33 0.2829123520552912 1102 34-35 0.1114525632144776 1102 36-37 0.25500952057160475 1102 38-39 0.10636476073968737 1102 40-41 0.18324682647067903 1102 42-43 0.24282068600261653 1102 44-45 -0.16661124227670854 1102 46-47 -0.5540049688119453 1102 48-49 -0.06535108967306513 1102 50-51 0.07279828846908742 1102 52-53 0.13370604463730018 1102 54-55 0.038628146423370424 1102 56-57 -0.07579226533770367 1102 58-59 0.006677213955846639 1102 60-61 -0.18819860825911405 1102 62-63 -0.5301754374579701 1102 64-65 -0.3132468889454785 1102 66-67 -0.03303955163886485 1102 68-69 0.05613891598724052 1102 70-71 0.0967557473787437 1102 72-73 0.11050911706922761 1102 74-75 -0.041417217281875196 1102 76-77 -0.03279055927385599 1102 78-79 -0.012717509110821368 1102 80-81 0.05765689019156639 1102 82-83 -0.05127435116084911 1102 84-85 0.14961030814757947 1102 86-87 0.07517754030853752 1102 88-89 -0.08587730325422172 1102 90-91 0.0224621449209792 1102 92-93 0.00815354182377348 1102 94-95 0.02017097362621101 1102 96-97 -0.03971422527067858 1102 98-99 -0.17923430284314534 1102 100-101 -0.03238370326244322 1103 1 0.2575087456607186 1103 2 0.16752662063116475 1103 3 0.12370839269384248 1103 4 0.15286896427103613 1103 5 0.10768644229111857 1103 6 0.30951656197916577 1103 7 0.23587773004618384 1103 8 0.2755903407391713 1103 9 0.32164690809815966 1103 10-11 0.34958469652536905 1103 12-13 0.22542282619986764 1103 14-15 0.368941100031293 1103 16-17 0.340298726686143 1103 18-19 0.299179050050995 1103 20-21 0.08237147547922774 1103 22-23 0.18625365291866558 1103 24-25 0.24045134307716154 1103 26-27 0.21054558807945511 1103 28-29 0.102096458051669 1103 30-31 0.24141623790603006 1103 32-33 0.13446402647544176 1103 34-35 0.2573789872307941 1103 36-37 0.21771242742520514 1103 38-39 0.21050978953773125 1103 40-41 0.1375100076010085 1103 42-43 0.006784607435143641 1103 44-45 -0.04604291169516017 1103 46-47 0.19517268585149594 1103 48-49 0.2075992991067963 1103 50-51 0.4201814567379145 1103 52-53 0.14520548194638394 1103 54-55 0.08963780020896905 1103 56-57 0.1925073107939923 1103 58-59 0.2682104637574412 1103 60-61 0.3261015241837768 1103 62-63 0.3324367498402765 1103 64-65 0.4168413807990987 1103 66-67 0.08395996605464262 1103 68-69 0.027821272501057592 1103 70-71 0.13814989386211352 1103 72-73 0.06733821980051857 1103 74-75 0.03331604681028466 1103 76-77 -0.23350789449218468 1103 78-79 0.013326403747363713 1103 80-81 0.11393044729129542 1103 82-83 -0.08869074498834806 1103 84-85 -0.1169999193020459 1103 86-87 0.0743022433327809 1103 88-89 0.20162330281875995 1103 90-91 0.04877017800363603 1103 92-93 -0.03453632489121006 1103 94-95 -0.16341873921003014 1103 96-97 0.015948155077754933 1103 98-99 -0.010151425234159461 1103 100-101 -0.05034132663976365 1104 1 0.09023877007198777 1104 2 0.3195992747902352 1104 3 0.24293072446160124 1104 4 0.12750694860076806 1104 5 0.09216836667074091 1104 6 0.06138985134067809 1104 7 0.08030224609541392 1104 8 0.23637101642091096 1104 9 0.3360875164275825 1104 10-11 0.3588989980391517 1104 12-13 0.3035740693800548 1104 14-15 0.3617838552915984 1104 16-17 0.1958317061590975 1104 18-19 0.4591632366874947 1104 20-21 0.39207419879750915 1104 22-23 0.33751866136498876 1104 24-25 0.28330658895434624 1104 26-27 0.3334587152327231 1104 28-29 0.4309850114320781 1104 30-31 0.420756636320327 1104 32-33 0.1957893173067191 1104 34-35 0.3116752743646103 1104 36-37 0.2710416012618424 1104 38-39 0.32286228202213607 1104 40-41 0.2957309556994545 1104 42-43 0.22539314058802518 1104 44-45 0.2737887317668495 1104 46-47 0.48402305189933514 1104 48-49 0.302094453838869 1104 50-51 0.32861583673643224 1104 52-53 0.36316250209751644 1104 54-55 0.2423568134744869 1104 56-57 0.21141574002010088 1104 58-59 0.1692168679271262 1104 60-61 0.23551241575916038 1104 62-63 0.3941379718591733 1104 64-65 0.2737298347396475 1104 66-67 0.18006434880594924 1104 68-69 0.08988963376850734 1104 70-71 0.19329778640400264 1104 72-73 0.3323763768160575 1104 74-75 0.08850517051467932 1104 76-77 0.27507516022762246 1104 78-79 0.2126899886271687 1104 80-81 0.3176993999833897 1104 82-83 0.26966141522139253 1104 84-85 0.29865567437212803 1104 86-87 0.54272311406217 1104 88-89 0.583708544609209 1104 90-91 0.5249861638104107 1104 92-93 0.5266349554212759 1104 94-95 0.27547031136969125 1104 96-97 0.4617485792736602 1104 98-99 0.6210394641341459 1104 100-101 0.5604592370144807 1105 1 0.27864456717344055 1105 2 0.33790591094584244 1105 3 0.25589792507182096 1105 4 0.08936812327658572 1105 5 0.015127939515892308 1105 6 0.19945239901420564 1105 7 0.296167997430274 1105 8 0.2966303604331131 1105 9 0.4276206972056187 1105 10-11 0.3436434679094873 1105 12-13 0.3001415751008736 1105 14-15 0.3835229857263798 1105 16-17 0.4177996695458148 1105 18-19 0.1933356241779549 1105 20-21 0.17735261222237142 1105 22-23 0.19716778417200231 1105 24-25 0.32335235554473485 1105 26-27 0.22514445131206173 1105 28-29 0.03777372233978582 1105 30-31 0.1072554921555735 1105 32-33 0.32622409991542156 1105 34-35 0.3746043361495026 1105 36-37 0.33397066304674183 1105 38-39 0.39913993267049364 1105 40-41 0.30412149727078486 1105 42-43 0.20746789268566346 1105 44-45 0.25090543657234576 1105 46-47 0.5377987956064274 1105 48-49 0.3985856819090472 1105 50-51 0.295435058704399 1105 52-53 0.3444744776886637 1105 54-55 0.2701981559611468 1105 56-57 0.3117208506226916 1105 58-59 0.16730992666091282 1105 60-61 0.2641165347522971 1105 62-63 0.20039273921233303 1105 64-65 0.34886332062828274 1105 66-67 0.21820317413012447 1105 68-69 0.2977462317852826 1105 70-71 0.34089504040857577 1105 72-73 0.45098812357426254 1105 74-75 0.4241268333674668 1105 76-77 0.43373267357621614 1105 78-79 0.39804467970268576 1105 80-81 0.5690342588697348 1105 82-83 0.24067590797501737 1105 84-85 0.5023170016032452 1105 86-87 0.5446300553283763 1105 88-89 0.5291700243956328 1105 90-91 0.46739653757090593 1105 92-93 0.4370087159094531 1105 94-95 0.3612826683490944 1105 96-97 0.3877592581447473 1105 98-99 0.34491436878708015 1105 100-101 0.5021068342684885 1106 1 0.23028051911238023 1106 2 0.1101917350764765 1106 3 0.1603918337406327 1106 4 0.1878276718490426 1106 5 0.23176316327390367 1106 6 0.1833063119684084 1106 7 0.04855690519792688 1106 8 0.09533882970750795 1106 9 0.2575421820028154 1106 10-11 0.2764111636827451 1106 12-13 0.3397309033721001 1106 14-15 0.24921517332141718 1106 16-17 0.35460918801454966 1106 18-19 0.4152793148667797 1106 20-21 0.354109746303088 1106 22-23 0.47880473491851916 1106 24-25 0.26369424904952865 1106 26-27 0.27308665662336296 1106 28-29 0.2336038960374438 1106 30-31 0.2978759636931798 1106 32-33 0.19704870763235505 1106 34-35 0.37383542171941286 1106 36-37 0.391604928730672 1106 38-39 0.4058495201629668 1106 40-41 0.3924356161132607 1106 42-43 0.32996773505262667 1106 44-45 0.4437380715773642 1106 46-47 0.5099016538142536 1106 48-49 0.4341284445438234 1106 50-51 0.0524966003164522 1106 52-53 0.29402799954552705 1106 54-55 0.5068424526862216 1106 56-57 0.3622426578282827 1106 58-59 0.4286211433815126 1106 60-61 0.6421621305968088 1106 62-63 0.6355525512133156 1106 64-65 0.6629525894732424 1106 66-67 0.4806725054368215 1106 68-69 0.3303430700850356 1106 70-71 0.30310384475780694 1106 72-73 0.449274805898213 1106 74-75 0.4347317703784199 1106 76-77 0.3381682719707584 1106 78-79 0.30042881662971865 1106 80-81 0.3518137003968107 1106 82-83 0.40770220116576894 1106 84-85 0.4537205494346921 1106 86-87 0.29645064716929426 1106 88-89 0.36741258062878046 1106 90-91 0.35268990520964394 1106 92-93 0.5221339044413504 1106 94-95 0.25370188629950974 1106 96-97 0.46481614609471933 1106 98-99 0.3019567154380347 1106 100-101 0.4329617298484578 1107 1 0.3081149862031687 1107 2 0.25421997592834344 1107 3 0.23059061155338156 1107 4 0.468726990972435 1107 5 0.3722965520465138 1107 6 0.2712458320004245 1107 7 0.231213604545772 1107 8 0.4526930281182615 1107 9 0.4667807024169335 1107 10-11 0.46938185016377076 1107 12-13 0.4583609379473401 1107 14-15 0.446650985678815 1107 16-17 0.38856332721864106 1107 18-19 0.5180002809204183 1107 20-21 0.4680620916801175 1107 22-23 0.5124934291938743 1107 24-25 0.47626742138083245 1107 26-27 0.45504345235053734 1107 28-29 0.43637634389073554 1107 30-31 0.4773807637358942 1107 32-33 0.47406408010809287 1107 34-35 0.524483973152222 1107 36-37 0.5720566724498184 1107 38-39 0.7385041197999485 1107 40-41 0.5531918127229218 1107 42-43 0.5596462926497736 1107 44-45 0.6513587777334564 1107 46-47 0.9143865843708525 1107 48-49 0.8042338500290853 1107 50-51 0.4782627485187092 1107 52-53 0.4934059148385259 1107 54-55 0.498471864606465 1107 56-57 0.5773168190974829 1107 58-59 0.6792472978984563 1107 60-61 0.6376210522164172 1107 62-63 0.585104016681889 1107 64-65 0.6909712867504041 1107 66-67 0.598019383374158 1107 68-69 0.759222234057944 1107 70-71 0.760480232705568 1107 72-73 0.5889828268455446 1107 74-75 0.7519553097443747 1107 76-77 0.7039861197204615 1107 78-79 0.7122077798080468 1107 80-81 0.593459054566182 1107 82-83 0.8361564977185694 1107 84-85 0.6130650145190941 1107 86-87 0.5447803401397167 1107 88-89 0.5631600699189505 1107 90-91 0.5971880146922004 1107 92-93 0.7652031736434743 1107 94-95 0.5795132430911956 1107 96-97 0.6257322836428898 1107 98-99 0.5739273381500603 1107 100-101 0.7788338797501879 1108 1 0.1479204555450444 1108 2 0.24959560852455098 1108 3 0.12649646039063356 1108 4 0.014211823875264429 1108 5 0.016226745644516427 1108 6 0.09806646469887426 1108 7 0.11149824852384427 1108 8 0.13436777529174293 1108 9 0.15026689290476014 1108 10-11 0.08735109851953382 1108 12-13 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-1.379161793188377 2316 96-97 -1.2200575985750604 2316 98-99 -0.8119759388233767 2316 100-101 -1.3240448621080034 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 9.0 7 256.0 8 1059.0 9 954.0 10 686.0 11 772.0 12 913.0 13 1335.0 14 1753.0 15 2208.0 16 2882.0 17 3783.0 18 4790.0 19 6053.0 20 7375.0 21 8960.0 22 10320.0 23 10543.0 24 10795.0 25 11374.0 26 12539.0 27 14123.0 28 16317.0 29 19411.0 30 23472.0 31 28617.0 32 35609.0 33 47134.0 34 65413.0 35 99819.0 36 169186.0 37 301507.0 38 385323.0 39 141919.0 40 133.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.00997962435528 18.528928418750034 13.075389885463338 23.385702071431346 2 15.348366409567554 19.298587399254703 41.58807522315625 23.764970968021494 3 19.216714809515604 24.112887539550858 29.474983369777917 27.19541428115562 4 12.256448320653183 15.81829209860883 37.95341810155015 33.971841479187844 5 13.351556380755964 37.83805495862417 35.00833472418538 13.802053936434483 6 32.512073850362476 36.76694232724721 17.326330027113816 13.394653795276506 7 28.797910806440896 31.966170347073025 21.139518593988793 18.096400252497286 8 25.776262092107665 37.166792179098856 19.222021340805014 17.83492438798846 9 25.803292281901726 16.492348625008912 19.847546984889668 37.8568121081997 10-11 25.242723151103924 26.339948435499554 28.038967228610577 20.378361184785945 12-13 25.438165710269185 23.5173416476062 28.584500581244644 22.45999206087997 14-15 22.401713927739532 25.474912348101768 25.80565333111824 26.317720393040457 16-17 22.53641985488412 28.70835662675928 26.363663707580464 22.391559810776133 18-19 22.391481417869933 27.78605815833662 28.194908907942402 21.627551515851046 20-21 22.976204911573777 26.82603527237792 28.27131078363601 21.926449032412297 22-23 22.69764160785771 26.672134160412675 28.11097860768222 22.51924562404739 24-25 22.44231149237706 26.96487768613085 28.080232591882222 22.512578229609865 26-27 22.579424904410985 26.999285586958717 27.91931692716718 22.50197258146312 28-29 22.515583739019526 26.984430770336242 28.10652216269548 22.39346332794875 30-31 22.723136618712093 26.97271964746411 27.944604661510546 22.359539072313247 32-33 22.419994721358186 27.088034479756683 28.02267881399144 22.469291984893687 34-35 22.59403789843727 27.114324050569945 28.04250826687818 22.249129784114604 36-37 22.75274261370153 26.9595575890149 27.8322262464573 22.455473550826273 38-39 22.66302643051884 27.14082089789421 27.815644125576398 22.38050854601055 40-41 22.72648758897344 27.115291340954663 27.896240142274596 22.2619809277973 42-43 22.65103893896536 27.02768246896725 27.787730888760226 22.533547703307168 44-45 22.724434621891877 27.159181044487564 27.54750933375028 22.568874999870282 46-47 22.76546958629384 27.11420621684248 27.508409885968938 22.61191431089474 48-49 22.673181370445754 27.12512995582453 27.477683209621034 22.724005464108682 50-51 22.64153878327719 27.344029506366375 27.36902142633692 22.645410284019516 52-53 22.800378887398537 27.375305944660315 27.238477812962376 22.585837354978775 54-55 22.68424035007231 27.368293314516407 27.25883521508378 22.688631120327504 56-57 22.755178163262975 27.46753240015895 27.182170980981326 22.595118455596747 58-59 22.813532679748732 27.247375651397572 27.24986523405016 22.689226434803537 60-61 22.79300097900501 27.333750765388192 27.30915456171364 22.564093693893152 62-63 22.59227889286105 27.50585551457195 27.347157564936996 22.554708027629992 64-65 22.739447306493453 27.347878530435366 27.286167580512206 22.626506582558978 66-67 22.7555920431525 27.360810153757413 27.234379247072138 22.649218556017953 68-69 22.762120034195885 27.1449894452022 27.34301294518975 22.749877575412164 70-71 22.609747959594703 27.0787758086851 27.392724211195997 22.918752020524206 72-73 22.702422963532477 27.280326454303587 27.21682080413295 22.80042977803099 74-75 22.60922510629318 27.362441577670992 27.12010350828032 22.90822980775551 76-77 22.650172017375883 27.213643953633383 27.20332671945536 22.932857309535375 78-79 22.691078010072378 27.108553771814087 27.086379043255597 23.11398917485794 80-81 22.688117132793995 27.24603577697665 27.036652748134966 23.029194342094392 82-83 22.754617936086927 27.315312810863347 27.14744444179359 22.782624811256134 84-85 22.780710384308815 27.147967625868187 27.068052416907722 23.00326957291528 86-87 22.65528877383776 27.238596611315597 27.200845459853625 22.90526915499302 88-89 22.682722730337662 27.034561455073565 27.104272203876278 23.178443610712495 90-91 22.658262740515063 26.998385181716344 27.171972094667296 23.1713799831013 92-93 22.78273826516859 27.045539246677237 27.201430392094778 22.970292096059396 94-95 22.671062594932923 27.06467320936318 27.227081796159407 23.037182399544495 96-97 22.706877612431587 26.9297460981868 27.23155985050728 23.13181643887433 98-99 22.79291980816356 26.83373068774581 27.054165583391875 23.319183920698755 100-101 22.820715880993227 26.889351245164473 27.030109118462338 23.259823755379962 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 48.0 1 43.5 2 72.5 3 129.5 4 120.0 5 100.0 6 154.0 7 316.5 8 532.5 9 674.5 10 648.0 11 556.0 12 562.0 13 634.0 14 691.5 15 746.0 16 863.0 17 925.5 18 980.0 19 1082.5 20 1208.0 21 1355.5 22 1579.0 23 1927.0 24 2331.0 25 2868.5 26 3685.5 27 5073.5 28 7386.5 29 10796.0 30 15488.0 31 21345.5 32 27605.5 33 33714.0 34 39333.5 35 43764.5 36 47667.5 37 50036.5 38 49810.0 39 48424.0 40 46198.5 41 44113.0 42 42860.0 43 42350.5 44 42888.0 45 43808.0 46 44471.0 47 45463.0 48 46897.0 49 48246.5 50 49364.5 51 49736.5 52 49115.0 53 48691.5 54 47767.0 55 45830.5 56 43387.0 57 40202.5 58 37120.0 59 33325.5 60 28034.0 61 22829.0 62 18339.0 63 14296.0 64 10872.0 65 8198.0 66 6185.5 67 4740.0 68 3639.5 69 2833.5 70 2367.0 71 1850.0 72 1358.0 73 1058.5 74 862.5 75 705.0 76 563.0 77 408.5 78 296.0 79 203.5 80 125.5 81 98.0 82 83.5 83 59.5 84 39.5 85 32.0 86 32.0 87 29.0 88 20.5 89 15.0 90 15.5 91 14.5 92 13.0 93 9.0 94 7.5 95 7.0 96 5.5 97 3.0 98 1.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.44260444317929004 2 0.3431808100642419 3 0.18537429301436703 4 0.21349480634155577 5 0.23373881225031817 6 0.18592703037706362 7 0.17784324644762606 8 0.1795014585357158 9 0.1774977855959407 10-11 0.18955436931975994 12-13 0.17946691245054727 14-15 0.18703250510245678 16-17 0.07727959252201622 18-19 0.03326788001730068 20-21 3.8000693685390186E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.13234605228066343 46-47 0.20112730785121968 48-49 0.08135603057190353 50-51 0.06042110295977039 52-53 0.0692303546777472 54-55 0.07783232988471281 56-57 0.11272387590493471 58-59 0.09095984224875668 60-61 0.1379770641631349 62-63 0.2351897478273967 64-65 0.13082602453324785 66-67 0.10315461031325007 68-69 0.10736923270381153 70-71 0.08598520598448742 72-73 0.07005946072179209 74-75 0.14143167267998855 76-77 0.21808943566897113 78-79 0.13839161718515733 80-81 0.08211604444561134 82-83 0.08764341807257717 84-85 0.09911271834853132 86-87 0.07106129719167964 88-89 0.09444899685077889 90-91 0.11528028620740641 92-93 0.16744487481189657 94-95 0.13220786793998932 96-97 0.08646885117684694 98-99 0.0766923090741511 100-101 0.061250209003815274 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1447342.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.70439067684272 #Duplication Level Percentage of deduplicated Percentage of total 1 74.079677242484 44.969616685421634 2 14.526262067118493 17.63615775193124 3 5.129488504351863 9.341474224216475 4 2.39523404295843 5.816048924248881 5 1.2143215090229296 3.6857323645510554 6 0.749838641811049 2.7311098714254634 7 0.4511059660818631 1.9168878961181586 8 0.31143932160260324 1.5124587400556233 9 0.2274049041917122 1.2424028531295317 >10 0.8754291513202139 8.710074843931961 >50 0.030557940991748304 1.2327998698781975 >100 0.008784413753439914 0.7583807579282663 >500 1.14073577939102E-4 0.039540419804798145 >1k 3.42220733817306E-4 0.4073147973587088 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2501 0.1727995180130197 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2152 0.14868635056538124 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 6.909217033707307E-5 0.0 0.0 0.0 8 0.0 6.909217033707307E-5 0.0 0.0 0.0 9 0.0 6.909217033707307E-5 0.0 0.0 0.0 10-11 0.0 6.909217033707307E-5 0.0 6.909217033707307E-5 0.0 12-13 0.0 6.909217033707307E-5 0.0 5.527373626965845E-4 0.0 14-15 0.0 6.909217033707307E-5 0.0 0.001105474725393169 0.0 16-17 0.0 6.909217033707307E-5 0.0 0.003040055494831215 0.0 18-19 0.0 6.909217033707307E-5 0.0 0.005458281456628772 0.0 20-21 0.0 6.909217033707307E-5 0.0 0.009811088187864376 0.0 22-23 0.0 6.909217033707307E-5 0.0 0.02010582156808826 0.0 24-25 0.0 6.909217033707307E-5 0.0 0.03136784533303117 0.0 26-27 0.0 6.909217033707307E-5 0.0 0.03672248853415433 0.0 28-29 0.0 6.909217033707307E-5 0.0 0.04062619615819896 0.0 30-31 0.0 6.909217033707307E-5 0.0 0.04522082548561432 0.0 32-33 0.0 6.909217033707307E-5 0.0 0.05365007026673724 0.0 34-35 0.0 6.909217033707307E-5 0.0 0.06536119313887112 0.0 36-37 0.0 6.909217033707307E-5 0.0 0.08432699389639767 0.0 38-39 0.0 6.909217033707307E-5 0.0 0.11621303050695689 0.0 40-41 0.0 6.909217033707307E-5 0.0 0.15127730695302147 0.0 42-43 0.0 6.909217033707307E-5 0.0 0.18603066863256923 0.0 44-45 0.0 6.909217033707307E-5 0.0 0.22582775874672328 0.0 46-47 0.0 6.909217033707307E-5 0.0 0.27892509165076396 0.0 48-49 0.0 6.909217033707307E-5 0.0 0.3354770330716582 0.0 50-51 0.0 6.909217033707307E-5 0.0 0.40052731144401255 0.0 52-53 0.0 6.909217033707307E-5 0.0 0.4620538891291761 0.0 54-55 0.0 6.909217033707307E-5 0.0 0.5288314717599572 0.0 56-57 0.0 6.909217033707307E-5 0.0 0.6130202813156808 0.0 58-59 0.0 6.909217033707307E-5 0.0 0.7055346973970216 0.0 60-61 0.0 6.909217033707307E-5 0.0 0.8013309915693734 0.0 62-63 0.0 6.909217033707307E-5 0.0 0.9024128367725113 0.0 64-65 0.0 6.909217033707307E-5 0.0 1.0094366086246374 0.0 66-67 0.0 6.909217033707307E-5 0.0 1.1318333883767622 0.0 68-69 0.0 6.909217033707307E-5 0.0 1.2806233771976492 0.0 70-71 0.0 6.909217033707307E-5 0.0 1.4336625344942662 0.0 72-73 0.0 6.909217033707307E-5 0.0 1.59644368780841 0.0 74-75 0.0 6.909217033707307E-5 0.0 1.7612285140623296 0.0 76-77 0.0 6.909217033707307E-5 0.0 1.9423536386009665 0.0 78-79 0.0 6.909217033707307E-5 0.0 2.1634485836796005 0.0 80-81 0.0 1.036382555056096E-4 0.0 2.4026456773865474 0.0 82-83 0.0 1.3818434067414614E-4 0.0 2.6630195213018073 0.0 84-85 0.0 1.3818434067414614E-4 0.0 2.92284062785437 0.0 86-87 0.0 1.3818434067414614E-4 0.0 3.193647389490528 0.0 88-89 0.0 1.3818434067414614E-4 0.0 3.5024548448120765 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2175 0.0 40.95487 1 GTATCAA 3430 0.0 37.080055 1 TCAACGC 3985 0.0 31.263891 4 AACGCAG 4065 0.0 30.86866 6 CAACGCA 4040 0.0 30.602861 5 ATCAACG 4070 0.0 30.494125 3 TATACCG 110 2.7108108E-6 30.260448 5 TATCAAC 4330 0.0 28.795837 2 TACCGTG 260 0.0 27.419672 7 ACGCAGA 4755 0.0 26.287518 7 CGCAGAG 4740 0.0 25.86936 8 GTACATG 4795 0.0 24.736252 1 TACATGG 4790 0.0 23.347946 2 ACATGGG 4560 0.0 22.941822 3 GCAGAGT 5600 0.0 21.726826 9 TATACTG 805 0.0 20.674843 5 GTATACC 285 1.9390427E-9 20.021954 4 ACCGTGC 340 4.1836756E-11 19.570118 8 ATACCGT 245 1.7448656E-7 19.400297 6 GAGTACT 3000 0.0 19.16807 12-13 >>END_MODULE