FastQCFastQC Report
Fri 10 Feb 2017
SRR2031624_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031624_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1447342
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23990.16575211663863826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23430.1618829550997622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19250.046.9021761
GTATCAA31850.036.553311
TCAACGC36050.031.621394
ATCAACG36800.030.9769333
CAACGCA37400.030.4778675
AACGCAG37600.030.1894386
TATCAAC38800.029.6260412
TATACCG1205.38763E-627.7052335
GTACATG46800.026.7042241
ACGCAGA44300.025.623547
TACATGG47300.025.3063412
CGCAGAG44550.025.159928
ACATGGG46300.024.1080383
GTGTACG1553.895096E-521.4603311
GAGTACA34650.021.3939741
CCGTATG2005.3938857E-721.3726069
CATGGGG28750.020.4860784
GCAGAGT54200.020.4174549
AGTACAT34450.020.4062142
CTATACT6200.019.1524034
Produced by FastQC (version 0.11.5)