FastQCFastQC Report
Fri 10 Feb 2017
SRR2031623_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031623_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241521
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18350.027.5026441
GTACATG30950.027.0742661
GTATCAA24900.026.1954521
TACATGG32100.024.3048112
ACATGGG31000.024.2181023
TCAACGC26350.023.8043084
TATACCG1000.001649984223.7592265
ATCAACG26550.023.6240373
CAACGCA27250.023.018115
CATGGGG19250.022.9569664
AACGCAG28750.021.9753516
TATCAAC29700.021.4635622
CCTATAC4300.020.9956783
ACTGCGT2056.8258305E-720.8550228
TAGACGT1150.003715071620.6601965
CGCAGAG31000.019.9206588
GAGTACA24950.019.6549221
ACGCAGA31900.019.6548737
CCTAATA4700.019.2313252
AAGACCG2001.28057E-519.007385
Produced by FastQC (version 0.11.5)