##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031623_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1241521 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.806364934624547 33.0 31.0 34.0 30.0 34.0 2 32.06269487185477 34.0 31.0 34.0 30.0 34.0 3 32.21662138618678 34.0 31.0 34.0 30.0 34.0 4 35.30016971118491 37.0 35.0 37.0 33.0 37.0 5 34.995505512995756 37.0 35.0 37.0 32.0 37.0 6 35.20962512917623 37.0 35.0 37.0 32.0 37.0 7 35.35527067202246 37.0 35.0 37.0 33.0 37.0 8 35.43220452976631 37.0 35.0 37.0 33.0 37.0 9 37.358058381614164 39.0 38.0 39.0 34.0 39.0 10-11 37.30972935616876 39.0 37.5 39.0 34.5 39.0 12-13 37.328639225595055 39.0 38.0 39.0 34.5 39.0 14-15 38.73774829422942 41.0 39.0 41.0 35.0 41.0 16-17 38.6897511197958 41.0 38.0 41.0 34.0 41.0 18-19 38.60544968631219 41.0 38.5 41.0 34.0 41.0 20-21 38.59428676599107 40.0 38.0 41.0 34.0 41.0 22-23 38.53243239542465 40.0 38.0 41.0 34.0 41.0 24-25 38.45081718311651 40.0 38.0 41.0 34.0 41.0 26-27 38.36040066982355 40.0 38.0 41.0 34.0 41.0 28-29 38.244714346354186 40.0 38.0 41.0 33.5 41.0 30-31 38.12125006343026 40.0 38.0 41.0 33.0 41.0 32-33 37.97849210766471 40.0 38.0 41.0 33.0 41.0 34-35 37.85822148799738 40.0 38.0 41.0 33.0 41.0 36-37 37.73335489290959 40.0 38.0 41.0 32.5 41.0 38-39 37.57099799359012 40.0 37.5 41.0 31.5 41.0 40-41 37.43178448048805 40.0 37.0 41.0 31.0 41.0 42-43 37.270759817997444 40.0 37.0 41.0 31.0 41.0 44-45 37.078452156669115 40.0 37.0 41.0 30.5 41.0 46-47 36.912075591149886 40.0 36.5 41.0 30.0 41.0 48-49 37.06735327070585 40.0 37.0 41.0 30.0 41.0 50-51 36.85157681585733 39.5 36.5 40.5 30.0 41.0 52-53 36.99647126387713 39.5 36.5 40.5 30.5 41.0 54-55 37.28589729855557 40.0 37.0 41.0 31.0 41.0 56-57 37.200756974710856 40.0 37.0 41.0 31.0 41.0 58-59 37.01305334343922 40.0 36.0 41.0 30.5 41.0 60-61 36.736494187371775 39.5 36.0 41.0 30.0 41.0 62-63 36.40642687477698 39.0 35.0 41.0 29.0 41.0 64-65 36.14441318350636 39.0 35.0 41.0 28.0 41.0 66-67 35.93335392635324 38.5 35.0 41.0 28.5 41.0 68-69 35.68285031022431 37.5 35.0 40.0 28.5 41.0 70-71 35.36712387466664 37.0 35.0 40.0 28.0 41.0 72-73 34.98820116615023 36.5 34.0 39.0 28.0 41.0 74-75 34.55799740801807 36.0 34.0 39.0 27.5 41.0 76-77 34.15098254479787 35.5 34.0 38.5 26.5 40.0 78-79 33.78633305437444 35.0 34.0 37.0 26.0 39.0 80-81 33.451783336729704 35.0 34.0 37.0 26.0 39.0 82-83 33.1214683440715 35.0 33.5 36.5 26.0 38.5 84-85 32.850516020268685 35.0 33.0 36.0 26.0 37.0 86-87 32.6078161384302 35.0 33.0 36.0 25.5 37.0 88-89 32.35459649897182 35.0 33.0 35.0 25.0 36.5 90-91 32.156623609266376 35.0 33.0 35.0 25.0 36.0 92-93 31.98864779572798 35.0 32.5 35.0 24.5 36.0 94-95 31.83700920081094 35.0 32.0 35.0 24.0 36.0 96-97 31.736787778861572 35.0 32.0 35.0 24.0 35.5 98-99 31.61976237212258 35.0 32.0 35.0 23.5 35.0 100-101 31.17059437576972 34.5 31.5 35.0 21.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05636879269274431 1101 2 -0.04795008578566495 1101 3 0.22905533306688142 1101 4 0.2246662383944198 1101 5 0.2511693512994384 1101 6 0.21957440143235374 1101 7 0.19187712781997135 1101 8 0.15328287709454713 1101 9 0.28497199966786724 1101 10-11 0.3370708433024774 1101 12-13 0.3927330897504149 1101 14-15 0.4729456282768325 1101 16-17 0.44423594893543594 1101 18-19 0.3899492664811035 1101 20-21 0.1872771552160799 1101 22-23 0.2997429953934798 1101 24-25 0.2294698549414207 1101 26-27 0.1455295893282127 1101 28-29 0.219114488502953 1101 30-31 0.3364551729882024 1101 32-33 0.393600090227217 1101 34-35 0.3680668569866228 1101 36-37 0.4049015046270483 1101 38-39 0.4029249739197738 1101 40-41 0.4653722386417556 1101 42-43 0.3212438386052341 1101 44-45 0.32532675704559466 1101 46-47 0.4042942370459528 1101 48-49 0.2256738769336124 1101 50-51 0.170487651261098 1101 52-53 0.25829582392611883 1101 54-55 0.2830303640140457 1101 56-57 0.3794789931293323 1101 58-59 0.451491958592257 1101 60-61 0.2743132152503662 1101 62-63 -0.08575304191717947 1101 64-65 0.1024687802008728 1101 66-67 0.20689095698914883 1101 68-69 0.04043140427874192 1101 70-71 0.0712340577173336 1101 72-73 0.10989126377116776 1101 74-75 -0.04172827506564403 1101 76-77 0.2001961019246039 1101 78-79 0.14390216986053161 1101 80-81 -0.012651399803594643 1101 82-83 0.07232918644871233 1101 84-85 -0.05252153663884229 1101 86-87 0.09203540900792717 1101 88-89 0.2630553435612981 1101 90-91 0.01418204418477842 1101 92-93 0.07220980946053857 1101 94-95 -0.2290192160618716 1101 96-97 -0.08090996579814913 1101 98-99 0.04540318151374123 1101 100-101 0.18484577228701582 1102 1 -0.04473620517338617 1102 2 0.018678517280186213 1102 3 0.08887747224125064 1102 4 0.19566292801459184 1102 5 0.25282454121374087 1102 6 0.12838324141528545 1102 7 0.2337586231427622 1102 8 0.37791579403516096 1102 9 0.3616009487664513 1102 10-11 0.3934828298764046 1102 12-13 0.5632609050150705 1102 14-15 0.4924935941810702 1102 16-17 0.5718903083711666 1102 18-19 0.6079758417951311 1102 20-21 0.6728620082098402 1102 22-23 0.30223731468941395 1102 24-25 0.6174013865976562 1102 26-27 0.5730386150153777 1102 28-29 0.3507482243877007 1102 30-31 0.3692071713272398 1102 32-33 0.5666106903633263 1102 34-35 0.4474121630525829 1102 36-37 0.2606952460483214 1102 38-39 0.43021830586896925 1102 40-41 0.49605957499711195 1102 42-43 0.4776246822330421 1102 44-45 0.14297038946655505 1102 46-47 -0.4474163181058941 1102 48-49 0.19710887468439608 1102 50-51 0.41960815139374574 1102 52-53 0.5075835732138643 1102 54-55 0.45365333828073773 1102 56-57 0.47743797671584787 1102 58-59 0.4940799374150515 1102 60-61 0.35166018227345575 1102 62-63 -0.09038411334986307 1102 64-65 0.2117747538793111 1102 66-67 0.4187321971704634 1102 68-69 0.5641865515156752 1102 70-71 0.34184319067464486 1102 72-73 0.26464286993977026 1102 74-75 0.42416443171774887 1102 76-77 0.43071445896118377 1102 78-79 0.5264730777350835 1102 80-81 0.4491984911960927 1102 82-83 0.357685081116351 1102 84-85 0.4197785429486913 1102 86-87 0.24184451428262577 1102 88-89 0.3219703002495393 1102 90-91 0.34576791220789715 1102 92-93 0.26383697072332524 1102 94-95 0.3445559941530121 1102 96-97 0.22558276174721215 1102 98-99 0.01794836762167762 1102 100-101 0.057664324255366495 1103 1 0.07156300798521897 1103 2 0.05025533035910712 1103 3 0.022132904008010712 1103 4 0.19804621795070432 1103 5 0.14207981646594448 1103 6 0.2615380058608636 1103 7 0.35881553752930273 1103 8 0.26055299327819625 1103 9 0.3695938629253277 1103 10-11 0.28286260534914476 1103 12-13 0.195489032104895 1103 14-15 0.36890133596050134 1103 16-17 0.3116946295959693 1103 18-19 0.2955158597231602 1103 20-21 0.04328573581758377 1103 22-23 0.15214963096266132 1103 24-25 0.24098564123261923 1103 26-27 0.15238705200403757 1103 28-29 0.33359209399684886 1103 30-31 0.20807098231457388 1103 32-33 0.2612801244920391 1103 34-35 0.41638492951862105 1103 36-37 0.4457043523501696 1103 38-39 0.4919793081313273 1103 40-41 0.18646309799763827 1103 42-43 0.08763930211608084 1103 44-45 0.13166754801580538 1103 46-47 0.4784189005164947 1103 48-49 0.30511192671837506 1103 50-51 0.3095544028699564 1103 52-53 0.19633391114304288 1103 54-55 0.042371306152567456 1103 56-57 0.09742951070085581 1103 58-59 0.20286147154234868 1103 60-61 0.4273207448721621 1103 62-63 0.3084363359892137 1103 64-65 0.2869373155958499 1103 66-67 0.11143986353832958 1103 68-69 0.06663031298747768 1103 70-71 0.2391847552511308 1103 72-73 0.3327898442287065 1103 74-75 0.2613803933574488 1103 76-77 -0.2118347364116815 1103 78-79 -0.29257474457819654 1103 80-81 0.006992382859557722 1103 82-83 -0.28354352263146154 1103 84-85 -0.2853720311751857 1103 86-87 0.016741890529395675 1103 88-89 0.11749825232761424 1103 90-91 -0.07622380674886386 1103 92-93 -0.3484124281184684 1103 94-95 -0.22787645558447167 1103 96-97 -0.062065215657774786 1103 98-99 0.10312608279362934 1103 100-101 0.13321639380474792 1104 1 0.319545004011097 1104 2 0.2782638539797091 1104 3 0.28555309040367405 1104 4 0.16109157244366656 1104 5 0.135508131746235 1104 6 0.20702597310447146 1104 7 0.1716145165681482 1104 8 0.20159098159668787 1104 9 0.29435484695524394 1104 10-11 0.428436544747143 1104 12-13 0.27293003914993363 1104 14-15 0.3005371652739939 1104 16-17 0.22543436813357687 1104 18-19 0.33526209133281526 1104 20-21 0.22666567921645964 1104 22-23 0.32292938502208557 1104 24-25 0.2664670183927953 1104 26-27 0.29436613434328507 1104 28-29 0.348035081412128 1104 30-31 0.44961384796383896 1104 32-33 0.44196423871122903 1104 34-35 0.4738900978835474 1104 36-37 0.30750745939051427 1104 38-39 0.40870925520993495 1104 40-41 0.27895006836568115 1104 42-43 0.24042344510416314 1104 44-45 0.1370424802750989 1104 46-47 0.44650801048725697 1104 48-49 0.4702742200674166 1104 50-51 0.3881791996478441 1104 52-53 0.36311027314936695 1104 54-55 0.27253206070039937 1104 56-57 0.3439195385999838 1104 58-59 0.2386929594293008 1104 60-61 0.48941137357281406 1104 62-63 0.47337742666474725 1104 64-65 0.4684472810062701 1104 66-67 0.24950336584988264 1104 68-69 0.28078460791545723 1104 70-71 0.2978352433187297 1104 72-73 0.2714163470687865 1104 74-75 0.22923533792715745 1104 76-77 0.20614715925759697 1104 78-79 0.25583058904059186 1104 80-81 0.23261785537188473 1104 82-83 0.26967767335159465 1104 84-85 0.24412151924062186 1104 86-87 0.17118442633998399 1104 88-89 0.06353650826370938 1104 90-91 0.15311778704775492 1104 92-93 0.19143750531165438 1104 94-95 0.05612352434420842 1104 96-97 0.17317943700803085 1104 98-99 0.13282410978286663 1104 100-101 0.14359242998664357 1105 1 0.1925394611279927 1105 2 0.3121054588313754 1105 3 0.28675541286045814 1105 4 0.22547280979239304 1105 5 0.12488306854003639 1105 6 0.16499525208020316 1105 7 0.2619452114270615 1105 8 0.3448029964740229 1105 9 0.22422525617829336 1105 10-11 0.35943463290996647 1105 12-13 0.3351642721115482 1105 14-15 0.3746674077771388 1105 16-17 0.689463015191734 1105 18-19 0.5045868965774432 1105 20-21 0.4484335747990116 1105 22-23 0.35692148083546726 1105 24-25 0.32026613262305403 1105 26-27 0.40486301938103253 1105 28-29 0.3677714694043104 1105 30-31 0.35376796034005764 1105 32-33 0.38565613916727415 1105 34-35 0.3673561415042528 1105 36-37 0.48047655376399945 1105 38-39 0.5178095841229933 1105 40-41 0.4957361111516647 1105 42-43 0.35591863661695555 1105 44-45 0.5226051398958447 1105 46-47 0.7134416548084843 1105 48-49 0.569851882232463 1105 50-51 0.5199109613472075 1105 52-53 0.5081835602411786 1105 54-55 0.49344877940349363 1105 56-57 0.5438658434248467 1105 58-59 0.44966724192853036 1105 60-61 0.4943819897108952 1105 62-63 0.5760836955965161 1105 64-65 0.45090244272586943 1105 66-67 0.4386981950606952 1105 68-69 0.44438032257496474 1105 70-71 0.4479529874471737 1105 72-73 0.4980420908954528 1105 74-75 0.3386539050605748 1105 76-77 0.26612683695812933 1105 78-79 0.4925793582251785 1105 80-81 0.567896067009535 1105 82-83 0.44282093593143657 1105 84-85 0.22536994410189237 1105 86-87 0.3532825031056248 1105 88-89 0.4614779524311601 1105 90-91 0.3707574145685797 1105 92-93 0.3705177366583925 1105 94-95 0.43388834429281076 1105 96-97 0.44003843781224816 1105 98-99 0.39963655871626713 1105 100-101 0.46443675250442595 1106 1 0.33084516296011657 1106 2 0.26243183998087716 1106 3 0.2301261055454944 1106 4 0.3004864027486889 1106 5 0.4314245097839162 1106 6 0.15915103186529933 1106 7 0.21928585842174186 1106 8 0.4094258632810792 1106 9 0.34060649579583213 1106 10-11 0.4599669360824592 1106 12-13 0.3307086362165279 1106 14-15 0.4038021447646969 1106 16-17 0.3996356532466052 1106 18-19 0.37391728201661323 1106 20-21 0.2581697362683144 1106 22-23 0.41545154905558235 1106 24-25 0.3810349081755646 1106 26-27 0.3705787298345413 1106 28-29 0.37252675113147404 1106 30-31 0.5083919686550402 1106 32-33 0.5367782715040974 1106 34-35 0.48685324718085354 1106 36-37 0.4712784030068491 1106 38-39 0.5180228658681685 1106 40-41 0.47750427689861397 1106 42-43 0.2985097995431829 1106 44-45 0.45877156574439937 1106 46-47 0.7879445731636352 1106 48-49 0.5159276483194546 1106 50-51 0.3044813788687577 1106 52-53 0.24492554640490027 1106 54-55 0.39487956947717606 1106 56-57 0.6219675157339637 1106 58-59 0.38357318167493304 1106 60-61 0.6499860426648567 1106 62-63 0.6619438668827868 1106 64-65 0.775459432509372 1106 66-67 0.5906148769975346 1106 68-69 0.4517137600458625 1106 70-71 0.5292125813707429 1106 72-73 0.37645948026681 1106 74-75 0.6387247987110882 1106 76-77 0.4444641705758343 1106 78-79 0.4472682522541973 1106 80-81 0.4480700268104272 1106 82-83 0.42280249156642924 1106 84-85 0.41232566260603676 1106 86-87 0.4601764600227085 1106 88-89 0.37711375732943253 1106 90-91 0.29087438909193253 1106 92-93 0.4636219773603685 1106 94-95 0.4420929325539049 1106 96-97 0.4412565469344045 1106 98-99 0.3217301443428795 1106 100-101 0.36350492155574443 1107 1 0.08242127646300546 1107 2 0.12975574124516953 1107 3 0.2065965768460245 1107 4 0.032410794738055415 1107 5 0.20863731634509008 1107 6 0.1956330897299594 1107 7 0.12677367549034102 1107 8 0.25040169052767425 1107 9 0.3715153595173888 1107 10-11 0.2970230684419093 1107 12-13 0.3520003677262977 1107 14-15 0.16319847909386453 1107 16-17 0.18773997208158733 1107 18-19 0.3088152748147692 1107 20-21 0.20395427161301427 1107 22-23 0.2514178912227294 1107 24-25 0.3195253038226511 1107 26-27 0.378842676309155 1107 28-29 0.4135064121676706 1107 30-31 0.39323557843773926 1107 32-33 0.4004103753742285 1107 34-35 0.36180354990823815 1107 36-37 0.36789884647691196 1107 38-39 0.20546180921895996 1107 40-41 0.40191543521424933 1107 42-43 0.3123909577452153 1107 44-45 0.15148977936883057 1107 46-47 0.5790406678099629 1107 48-49 0.40850415372084825 1107 50-51 0.1873157989157903 1107 52-53 0.4202184763289054 1107 54-55 0.39902645189627606 1107 56-57 0.3014676747652416 1107 58-59 0.1973447218928044 1107 60-61 0.31283761537054744 1107 62-63 0.28981352939570115 1107 64-65 0.4255025981404472 1107 66-67 0.2326724008806238 1107 68-69 0.3428817027491675 1107 70-71 0.2800588549272547 1107 72-73 0.22788096425576754 1107 74-75 0.35101095844664343 1107 76-77 0.4426282113332789 1107 78-79 0.4458781485430876 1107 80-81 0.4076334859677857 1107 82-83 0.4418365469774699 1107 84-85 0.3456915594856156 1107 86-87 0.3707746163958845 1107 88-89 0.36140444945625205 1107 90-91 0.3182781751721464 1107 92-93 0.270073819283283 1107 94-95 0.5039901756332057 1107 96-97 0.5341244203455062 1107 98-99 0.45588083215313446 1107 100-101 0.504678543947346 1108 1 0.2333780714017024 1108 2 0.2635395868237964 1108 3 0.23044449567581893 1108 4 0.0961558789586121 1108 5 0.10762174724442986 1108 6 0.13624226979678156 1108 7 0.12191909110570265 1108 8 0.23280642796721907 1108 9 0.24312407692261218 1108 10-11 0.2912529117287477 1108 12-13 0.288356826966357 1108 14-15 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-1.4183248360274305 2316 64-65 -1.8697597881815469 2316 66-67 -2.090914667739561 2316 68-69 -2.420781499241855 2316 70-71 -1.879522912270403 2316 72-73 -1.673325173291424 2316 74-75 -1.7915569287404551 2316 76-77 -1.4728846288484334 2316 78-79 -1.7484409340210263 2316 80-81 -1.7239709188154215 2316 82-83 -1.7466015363281642 2316 84-85 -2.354905915946297 2316 86-87 -1.756300247020036 2316 88-89 -1.5116527965314575 2316 90-91 -1.6664360210486677 2316 92-93 -1.8943757532788723 2316 94-95 -1.8812280605784117 2316 96-97 -1.8127186976068792 2316 98-99 -1.213662942936999 2316 100-101 -1.8431255633766277 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 8.0 7 89.0 8 375.0 9 409.0 10 391.0 11 495.0 12 812.0 13 1160.0 14 1652.0 15 2120.0 16 2762.0 17 3358.0 18 4172.0 19 5086.0 20 5923.0 21 6997.0 22 7793.0 23 8352.0 24 8948.0 25 9545.0 26 10655.0 27 12393.0 28 14194.0 29 16970.0 30 20093.0 31 24445.0 32 30275.0 33 39627.0 34 54925.0 35 81307.0 36 137724.0 37 257327.0 38 352117.0 39 118852.0 40 170.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.75841641849023 18.942444645863006 13.190227243299235 24.108911692347533 2 14.9831000649158 19.358651303530273 41.98136277907529 23.676885852478645 3 19.14687727997624 24.007302380537315 29.539291456021072 27.30652888346537 4 11.966122465784611 15.936019866036649 38.94091936930501 33.15693829887373 5 13.017701787620538 38.155096147789976 35.7835798599167 13.043622204672781 6 32.22927057608187 37.28608784078253 17.575097251141994 12.909544331993608 7 28.31089325603316 31.936921378491096 21.608648495099875 18.143536870375872 8 25.19573901464714 37.62433926481862 19.560101682605012 17.619820037929227 9 25.570288737548957 16.168463458507546 20.027793178082128 38.23345462586138 10-11 24.654323578311892 26.50211457442172 29.004374424948747 19.83918742231764 12-13 25.064993598488655 23.588873273478296 28.99277373673548 22.353359391297577 14-15 21.95471949069919 25.706801761375008 26.13859044802717 26.19988829989863 16-17 22.102889592774044 28.779192803710362 26.92235756201342 22.195560041502173 18-19 21.968610400242376 28.277391366006793 28.202897873410215 21.551100360340616 20-21 22.664016144771335 27.342125537598573 28.323304603684285 21.670553713945807 22-23 22.39788936312797 27.374969895797168 27.927276300602244 22.299864440472614 24-25 22.17413156926061 27.365988976424887 28.17362734903397 22.28625210528054 26-27 22.284882817125123 27.47718322928086 28.007258838150946 22.230675115443074 28-29 22.1900797489531 27.634168088981177 28.003795344581366 22.171956817484357 30-31 22.43594751921232 27.606137954976194 27.865013962711867 22.09290056309962 32-33 22.215572672552458 27.592968624775576 27.962394514470557 22.22906418820141 34-35 22.434336591970656 27.653901947691583 27.95172212149452 21.96003933884324 36-37 22.40775629248317 27.56872416978851 27.803798727528573 22.219720810199746 38-39 22.38862653148839 27.682858364860525 27.89010415450081 22.038410949150276 40-41 22.492934070386244 27.625992633229725 27.821760566273145 22.05931273011089 42-43 22.18492478177977 27.566267505744968 27.86287948411666 22.3859282283586 44-45 22.340129931041936 27.680941820591276 27.776803167255665 22.20212508111112 46-47 22.3945997492291 27.614258347293074 27.55840593004162 22.432735973436202 48-49 22.35511354589944 27.50136946766303 27.861240175404724 22.282276811032812 50-51 22.318368800956836 27.75525566670643 27.65604196151379 22.27033357082295 52-53 22.2883027749399 27.8938385493176 27.576305305855968 22.24155336988653 54-55 22.254887182285792 27.766031110620286 27.71947884028017 22.25960286681375 56-57 22.449638669244877 27.704578038895672 27.5079870143484 22.337796277511053 58-59 22.370966961737533 27.65483158386946 27.682040987439333 22.292160466953675 60-61 22.52733624968392 27.609440780970022 27.751279146533914 22.11194382281214 62-63 22.300096202018587 27.744977047562873 27.755231060454673 22.199695689963864 64-65 22.266595207475632 27.672208786657613 27.790850400667487 22.27034560519926 66-67 22.239364775023816 27.751665284556747 27.596293822190383 22.412676118229054 68-69 22.313282583577042 27.72505791390603 27.546216224279412 22.415443278237515 70-71 22.207263330287365 27.47863352940679 27.70565039000384 22.60845275030201 72-73 22.22056090133688 27.578535698337802 27.698110500497318 22.50279289982799 74-75 22.222343209966162 27.627510999802386 27.573187502772633 22.576958287458815 76-77 22.203109985184206 27.543576640362005 27.626998729901715 22.626314644552078 78-79 22.19904261454145 27.468816415087495 27.616497356503082 22.715643613867975 80-81 22.183482784709003 27.60573212901603 27.551398388448355 22.659386697826612 82-83 22.182720062300547 27.624706908081297 27.698592070465406 22.49398095915275 84-85 22.317333236582936 27.568702859377332 27.620262757950663 22.49370114608907 86-87 22.147076497237485 27.53060651211303 27.703623935165016 22.618693055484467 88-89 22.20475849942234 27.461585408622895 27.589896069573967 22.743760022380798 90-91 22.21107622611445 27.51348372095461 27.661374244873322 22.614065808057614 92-93 22.147666979830955 27.430448685496593 27.762518470171603 22.659365864500845 94-95 22.146432304436473 27.41091245773866 27.70843549178001 22.734219746044857 96-97 22.1513236974042 27.3534839212626 27.76991310250422 22.72527927882898 98-99 22.15894365910508 27.269755708472033 27.637093813089635 22.93420681933325 100-101 22.23383694858482 27.20034140310551 27.692700131048504 22.873121517261165 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 19.0 1 13.5 2 15.5 3 42.5 4 44.5 5 31.5 6 47.5 7 100.0 8 179.5 9 208.5 10 201.5 11 212.0 12 240.0 13 271.5 14 303.5 15 340.5 16 406.0 17 483.5 18 541.0 19 626.5 20 746.5 21 849.0 22 1035.5 23 1319.5 24 1695.0 25 2220.5 26 2877.0 27 3861.5 28 5459.5 29 7993.0 30 11876.5 31 16294.5 32 21261.0 33 26990.0 34 31919.0 35 35526.0 36 38990.0 37 41532.0 38 42150.5 39 42161.0 40 42832.5 41 44035.0 42 45025.5 43 46509.0 44 48059.0 45 48802.5 46 49507.5 47 50133.5 48 51254.0 49 51236.0 50 49201.5 51 46952.0 52 44284.5 53 41399.0 54 38582.5 55 35354.5 56 31663.5 57 27885.5 58 24016.0 59 20224.0 60 16376.0 61 12605.5 62 9633.5 63 7095.5 64 4968.0 65 3586.5 66 2555.5 67 1845.5 68 1326.0 69 960.0 70 711.5 71 525.5 72 369.5 73 243.5 74 178.0 75 121.5 76 85.0 77 65.0 78 47.5 79 35.5 80 24.0 81 20.0 82 17.5 83 11.0 84 8.5 85 13.0 86 13.5 87 8.0 88 8.0 89 5.5 90 4.5 91 6.5 92 5.0 93 2.5 94 2.5 95 1.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4704713009284579 2 0.3651972056856066 3 0.19919115343195967 4 0.22746292652319214 5 0.25090191788942756 6 0.19822459708696027 7 0.19081433177529822 8 0.19097542449946478 9 0.19073378541321492 10-11 0.20144645157029162 12-13 0.19012968769759028 14-15 0.2004798952252922 16-17 0.08892318373994479 18-19 0.03809842926539301 20-21 3.6245862937477497E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.13769400598137285 46-47 0.2047085792346646 48-49 0.08662761242057121 50-51 0.06161796699371174 52-53 0.06955178365891515 54-55 0.07893543484161766 56-57 0.11139561876118084 58-59 0.09190339913702628 60-61 0.1393049332230385 62-63 0.24014897855130923 64-65 0.1331028633426257 66-67 0.10209251394056162 68-69 0.10616010522576742 70-71 0.08735252967932078 72-73 0.07047806682287291 74-75 0.13902302095574703 76-77 0.21268266908090963 78-79 0.1358011664724157 80-81 0.08372794338557302 82-83 0.0871914369551542 84-85 0.09794437629327252 86-87 0.07204872088349694 88-89 0.09440033636160806 90-91 0.11393282916680426 92-93 0.1629050173134405 94-95 0.13024346748866913 96-97 0.08811772011911195 98-99 0.07849242985015961 100-101 0.06113468882121204 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1241521.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.1672299292383 #Duplication Level Percentage of deduplicated Percentage of total 1 75.10675978826731 48.944994843620854 2 14.564417645430547 18.98245506970456 3 4.922804757262538 9.624166485398279 4 2.2453727028810406 5.852988768219362 5 1.1352694457383148 3.699118250103385 6 0.6583213359650352 2.5740586720894068 7 0.3848790872296461 1.75570527807148 8 0.24550402011685513 1.2799053542005956 9 0.16553301472518875 0.9708595228328729 >10 0.5588329538548584 5.517070118096008 >50 0.010198650735676147 0.44352619631123097 >100 0.0017348506241280754 0.16820953329267968 >500 2.478314458779165E-4 0.10594720664097476 >1k 1.2391572293895826E-4 0.08099470141836715 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.054636208328333E-5 2 0.0 0.0 0.0 0.0 8.054636208328333E-5 3 0.0 0.0 0.0 0.0 8.054636208328333E-5 4 0.0 0.0 0.0 0.0 8.054636208328333E-5 5 0.0 0.0 0.0 0.0 8.054636208328333E-5 6 0.0 0.0 0.0 0.0 8.054636208328333E-5 7 0.0 0.0 0.0 0.0 8.054636208328333E-5 8 0.0 0.0 0.0 0.0 8.054636208328333E-5 9 0.0 0.0 0.0 0.0 8.054636208328333E-5 10-11 0.0 0.0 0.0 1.6109272416656666E-4 8.054636208328333E-5 12-13 0.0 0.0 0.0 1.6109272416656666E-4 8.054636208328333E-5 14-15 0.0 0.0 0.0 2.8191226729149167E-4 8.054636208328333E-5 16-17 0.0 0.0 0.0 6.443708966662666E-4 8.054636208328333E-5 18-19 0.0 0.0 0.0 0.0010068295260410418 1.6109272416656666E-4 20-21 0.0 0.0 0.0 0.0021344785952070082 1.6109272416656666E-4 22-23 0.0 0.0 0.0 0.005638245345829833 1.6109272416656666E-4 24-25 0.0 0.0 0.0 0.0086990071049946 2.4163908624985E-4 26-27 0.0 0.0 0.0 0.010350207527701907 2.4163908624985E-4 28-29 0.0 0.0 0.0 0.011800042045201006 2.4163908624985E-4 30-31 0.0 0.0 0.0 0.014820530623324132 2.4163908624985E-4 32-33 0.0 0.0 0.0 0.02162669821936157 2.4163908624985E-4 34-35 0.0 8.054636208328333E-5 0.0 0.03169499347977199 2.8191226729149167E-4 36-37 0.0 8.054636208328333E-5 0.0 0.04812645134476179 3.221854483331333E-4 38-39 0.0 8.054636208328333E-5 0.0 0.07668013670328573 3.221854483331333E-4 40-41 0.0 8.054636208328333E-5 0.0 0.10998605742472338 3.221854483331333E-4 42-43 0.0 8.054636208328333E-5 0.0 0.1427684267926197 3.221854483331333E-4 44-45 0.0 8.054636208328333E-5 0.0 0.18255832966176166 4.027318104164166E-4 46-47 0.0 8.054636208328333E-5 0.0 0.22806702423881675 4.832781724997E-4 48-49 0.0 8.054636208328333E-5 0.0 0.2798986082394096 4.832781724997E-4 50-51 0.0 8.054636208328333E-5 0.0 0.3369657057754158 4.832781724997E-4 52-53 0.0 8.054636208328333E-5 0.0 0.3944355351218385 5.638245345829833E-4 54-55 0.0 8.054636208328333E-5 0.0 0.4587518052453402 5.638245345829833E-4 56-57 0.0 8.054636208328333E-5 0.0 0.5375261473627913 5.638245345829833E-4 58-59 0.0 8.054636208328333E-5 0.0 0.622301193455447 5.638245345829833E-4 60-61 1.20819543124925E-4 8.054636208328333E-5 0.0 0.7122312067214328 5.638245345829833E-4 62-63 1.6109272416656666E-4 8.054636208328333E-5 0.0 0.8055844403759582 5.638245345829833E-4 64-65 1.6109272416656666E-4 8.054636208328333E-5 0.0 0.90473701210048 5.638245345829833E-4 66-67 1.6109272416656666E-4 8.054636208328333E-5 0.0 1.0232207107249898 5.638245345829833E-4 68-69 1.6109272416656666E-4 8.054636208328333E-5 0.0 1.1583372331196975 5.638245345829833E-4 70-71 1.6109272416656666E-4 8.054636208328333E-5 0.0 1.3101268524656449 5.638245345829833E-4 72-73 1.6109272416656666E-4 8.054636208328333E-5 0.0 1.46578269719159 5.638245345829833E-4 74-75 1.6109272416656666E-4 8.054636208328333E-5 0.0 1.6233313814264922 5.638245345829833E-4 76-77 1.6109272416656666E-4 8.054636208328333E-5 0.0 1.7983988994145084 5.638245345829833E-4 78-79 1.6109272416656666E-4 8.054636208328333E-5 0.0 2.007618074925837 5.638245345829833E-4 80-81 1.6109272416656666E-4 8.054636208328333E-5 0.0 2.2340338987419464 5.638245345829833E-4 82-83 1.6109272416656666E-4 8.054636208328333E-5 0.0 2.4743842431984637 5.638245345829833E-4 84-85 1.6109272416656666E-4 8.054636208328333E-5 0.0 2.713405572680607 5.638245345829833E-4 86-87 1.6109272416656666E-4 8.054636208328333E-5 0.0 2.9753423421754444 6.040977156246249E-4 88-89 1.6109272416656666E-4 8.054636208328333E-5 0.0 3.2756594532029664 6.443708966662666E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1835 0.0 27.502644 1 GTACATG 3095 0.0 27.074266 1 GTATCAA 2490 0.0 26.195452 1 TACATGG 3210 0.0 24.304811 2 ACATGGG 3100 0.0 24.218102 3 TCAACGC 2635 0.0 23.804308 4 TATACCG 100 0.0016499842 23.759226 5 ATCAACG 2655 0.0 23.624037 3 CAACGCA 2725 0.0 23.01811 5 CATGGGG 1925 0.0 22.956966 4 AACGCAG 2875 0.0 21.975351 6 TATCAAC 2970 0.0 21.463562 2 CCTATAC 430 0.0 20.995678 3 ACTGCGT 205 6.8258305E-7 20.855022 8 TAGACGT 115 0.0037150716 20.660196 5 CGCAGAG 3100 0.0 19.920658 8 GAGTACA 2495 0.0 19.654922 1 ACGCAGA 3190 0.0 19.654873 7 CCTAATA 470 0.0 19.231325 2 AAGACCG 200 1.28057E-5 19.00738 5 >>END_MODULE