FastQCFastQC Report
Fri 10 Feb 2017
SRR2031623_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031623_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241521
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22700.029.7298531
CCCGTAC700.00839260527.142343
TCAACGC24300.026.9747944
ATCAACG24650.026.784483
CAACGCA24650.026.5896425
AACGCAG25100.025.7344886
GGTATCA15750.025.6488951
GTACATG29050.025.5216481
ACTGCGT2059.586074E-1025.4852668
ACATGGG28800.024.244333
TACATGG30150.023.788932
ACCGCTC1401.7810027E-523.7476358
TATCAAC28250.023.7075142
ACGCAGA28950.022.4761717
CCATGCG1503.0358002E-522.164469
CGCAGAG30050.021.6534168
CGATTAG550.002879960221.58875810-11
GGACCGC1908.2592305E-619.9980076
GTGTAGC8100.019.949141
GTAGGAC3153.2196112E-1019.60283
Produced by FastQC (version 0.11.5)