FastQCFastQC Report
Fri 10 Feb 2017
SRR2031622_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031622_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1692958
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC19560.1155374203022166No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42350.033.96571
TCAACGC42750.032.58424
CAACGCA43250.032.2075045
ATCAACG43700.031.9846383
AACGCAG44850.031.1525236
GGTATCA27400.029.8995671
TATCAAC48400.029.1018772
CGCAGAG50600.027.331518
ACGCAGA51100.027.2492837
GTACATG46300.022.6323011
GCAGAGT60050.022.238969
ACATGGG43750.021.6247233
GTATTAG8350.019.394541
TACACTG19700.019.0649835
GGCGTAG1250.005937295519.0522841
GCGTTAT1250.005937295519.0522841
GAGTACT27850.018.76994112-13
TAATACT18700.018.5590954
TACATGG54100.018.4712682
TACACCG1300.007526291518.2853034
Produced by FastQC (version 0.11.5)