FastQCFastQC Report
Fri 10 Feb 2017
SRR2031622_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031622_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1692958
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC25070.14808400444665493No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23650.031.1422621
GTATCAA32100.029.3095911
TCAACGC31450.027.6392384
ATCAACG32500.026.7462793
CAACGCA32650.026.6202565
AACGCAG34400.025.2660276
TAATACT20600.024.9030284
TATCAAC38600.023.3809382
GTACATG41800.022.9627911
TTAATAC20850.022.781883
CCGACCA1503.0369665E-522.1641529
ATACTCG1553.9112456E-521.449186
CGCAGAG39900.021.3071068
ACTTAAT18550.021.0048481
ACGCAGA41000.020.9671337
ACATGGG39200.020.4784133
CTTAATA20550.020.1095852
TACACTG19150.019.8410915
CATGGGG21650.019.7460524
ATACTTC24050.019.3533136
Produced by FastQC (version 0.11.5)