FastQCFastQC Report
Fri 10 Feb 2017
SRR2031621_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031621_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167791
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23820.20397485508965216No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14960.12810511469946248No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12670.1084954413931945No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11790.10095984641087317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG1001.26747E-633.3554151
TACATGG31350.024.9090462
GTACATG32350.024.8932021
GTATAGT3450.023.4800051
ACATGGG31250.022.8251463
CCATGCG1754.0272644E-621.727979
CTGATCG1553.8875467E-521.4651329
CATGGGG20300.021.0823384
TCTAGAC5400.020.2537943
AGAGTAC35000.019.41937310-11
ACACCGT2702.282286E-819.3640486
GAGTACT21050.018.7410212-13
TACACTG11750.018.6162535
TATACTG6450.018.4311585
GTAGGAC3408.785719E-1018.1817933
GCACCGT2101.9452638E-518.1066426
GTATCAA54900.017.8798221
GTACCTG10000.017.630721
TACGACT1350.00934100117.6119944
GTATAGG3902.8740033E-1017.1053431
Produced by FastQC (version 0.11.5)