##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031621_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1167791
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.937586434558924	33.0	31.0	34.0	30.0	34.0
2	32.17979587100774	34.0	31.0	34.0	30.0	34.0
3	32.3373223462075	34.0	31.0	34.0	30.0	34.0
4	35.41023522188474	37.0	35.0	37.0	33.0	37.0
5	35.107389079038974	37.0	35.0	37.0	32.0	37.0
6	35.32853310224176	37.0	35.0	37.0	32.0	37.0
7	35.48384257114501	37.0	35.0	37.0	33.0	37.0
8	35.56309733505396	37.0	35.0	37.0	33.0	37.0
9	37.494421518919054	39.0	38.0	39.0	35.0	39.0
10-11	37.45435013628295	39.0	38.0	39.0	35.0	39.0
12-13	37.47349525728491	39.0	38.0	39.0	35.0	39.0
14-15	38.903309325041896	41.0	39.0	41.0	36.0	41.0
16-17	38.8581274388996	41.0	39.0	41.0	35.0	41.0
18-19	38.778772057671276	41.0	39.0	41.0	35.0	41.0
20-21	38.7698993227384	41.0	39.0	41.0	34.5	41.0
22-23	38.70854630665933	41.0	39.0	41.0	34.0	41.0
24-25	38.624236271730126	41.0	38.0	41.0	34.0	41.0
26-27	38.520233928845144	40.0	38.0	41.0	34.0	41.0
28-29	38.41614081629332	40.0	38.0	41.0	34.0	41.0
30-31	38.280484264735726	40.0	38.0	41.0	34.0	41.0
32-33	38.1235786198044	40.0	38.0	41.0	33.0	41.0
34-35	37.97822726840676	40.0	38.0	41.0	33.0	41.0
36-37	37.84837055603271	40.0	38.0	41.0	33.0	41.0
38-39	37.677921819914694	40.0	38.0	41.0	32.0	41.0
40-41	37.52602349221736	40.0	37.0	41.0	31.5	41.0
42-43	37.33650970079406	40.0	37.0	41.0	31.0	41.0
44-45	37.113992572301036	40.0	37.0	41.0	30.5	41.0
46-47	36.921968485799255	40.0	36.5	41.0	30.0	41.0
48-49	37.064530810735825	40.0	37.0	41.0	30.5	41.0
50-51	36.818130555895706	39.5	36.5	40.5	30.5	41.0
52-53	36.94059853175782	39.5	36.5	40.5	30.5	41.0
54-55	37.2060843935259	40.0	37.0	41.0	31.0	41.0
56-57	37.07946242092977	40.0	36.0	41.0	31.0	41.0
58-59	36.86562878117745	39.5	36.0	41.0	30.5	41.0
60-61	36.55816408929337	39.0	35.0	41.0	29.5	41.0
62-63	36.227211889798774	39.0	35.0	41.0	29.0	41.0
64-65	35.94091451295651	38.5	35.0	41.0	28.5	41.0
66-67	35.69937086345074	37.5	35.0	40.5	28.5	41.0
68-69	35.42196634500523	37.0	35.0	40.0	28.0	41.0
70-71	35.09647274212595	36.5	34.5	39.5	28.0	41.0
72-73	34.72276888587085	36.0	34.0	39.0	28.0	41.0
74-75	34.31018007503055	35.5	34.0	39.0	27.0	40.5
76-77	33.92110617396435	35.0	34.0	37.5	26.5	39.5
78-79	33.57529643574921	35.0	34.0	37.0	26.0	39.0
80-81	33.26078810335069	35.0	34.0	36.5	26.0	39.0
82-83	32.95285372125663	35.0	33.0	36.0	26.0	38.0
84-85	32.705279883129776	35.0	33.0	36.0	25.5	37.0
86-87	32.491389726415086	35.0	33.0	35.0	25.0	37.0
88-89	32.258425523060204	35.0	33.0	35.0	25.0	36.0
90-91	32.068737899161746	35.0	33.0	35.0	25.0	36.0
92-93	31.898638112470465	35.0	32.5	35.0	24.0	36.0
94-95	31.753875479430825	35.0	32.0	35.0	24.0	36.0
96-97	31.656042904937614	35.0	32.0	35.0	23.0	35.0
98-99	31.554690865060614	35.0	32.0	35.0	23.0	35.0
100-101	31.0981485556919	34.5	31.5	35.0	21.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.045845430560490286
1101	2	0.15100168919596513
1101	3	0.18288611628205587
1101	4	0.14145769419472742
1101	5	0.13823376636068474
1101	6	0.12752663228690153
1101	7	0.09908650857847334
1101	8	0.051617793872758
1101	9	0.1398751840235377
1101	10-11	0.22295046873028213
1101	12-13	0.06097721146149837
1101	14-15	0.1489937196109139
1101	16-17	7.214004383016004E-4
1101	18-19	0.08730436997293367
1101	20-21	0.058269240518733056
1101	22-23	-0.030292366378468216
1101	24-25	0.19122489412565358
1101	26-27	0.11973659128002367
1101	28-29	0.19392162520160383
1101	30-31	0.1923490680261608
1101	32-33	0.22131180079119872
1101	34-35	0.15391399473526945
1101	36-37	0.26470478560113975
1101	38-39	0.07104815809194776
1101	40-41	0.1000390416580288
1101	42-43	0.12259419855901399
1101	44-45	-0.04113219592512962
1101	46-47	0.1776959290169131
1101	48-49	0.06097626959397218
1101	50-51	0.017725702895305062
1101	52-53	-0.0949424627936466
1101	54-55	-0.04001760264667098
1101	56-57	-0.008792078906573408
1101	58-59	0.12112772743466138
1101	60-61	-0.025581735929286253
1101	62-63	0.004885865405853451
1101	64-65	0.140712152378633
1101	66-67	0.15878279326411615
1101	68-69	-0.054042545532091424
1101	70-71	0.11430846365345104
1101	72-73	-0.012260286823895683
1101	74-75	-0.0659808788413585
1101	76-77	0.059194852598601244
1101	78-79	-0.13300054017256002
1101	80-81	0.09091056626701288
1101	82-83	0.18375261150175248
1101	84-85	0.06752093423965988
1101	86-87	0.03276835142980161
1101	88-89	0.09314894705916998
1101	90-91	0.07403921527816948
1101	92-93	-0.12865299675789643
1101	94-95	-0.2812321436875642
1101	96-97	-0.16640996264922236
1101	98-99	-0.20226941230016493
1101	100-101	-0.04800177422060514
1102	1	-0.0362612481571567
1102	2	-0.004364518759032876
1102	3	-0.07109471740630369
1102	4	0.04498657455157229
1102	5	0.11786635217022479
1102	6	0.22137996431990103
1102	7	0.09048271329972835
1102	8	0.24326345835451235
1102	9	0.18011006630009518
1102	10-11	0.2811787911390553
1102	12-13	0.24651573143624006
1102	14-15	0.10699414051009626
1102	16-17	0.2242034685931742
1102	18-19	0.322698911629125
1102	20-21	0.25110471513389143
1102	22-23	0.21776251476884312
1102	24-25	0.18936472504737623
1102	26-27	0.17486617176672326
1102	28-29	0.18639984880172733
1102	30-31	0.2189262850612863
1102	32-33	0.17367525236983283
1102	34-35	0.004709853497928407
1102	36-37	0.008258611934024884
1102	38-39	0.009361276287620512
1102	40-41	0.054920702259302345
1102	42-43	0.07613308810377362
1102	44-45	-0.19893140648251517
1102	46-47	-0.6052355824731208
1102	48-49	-0.14712039349677042
1102	50-51	-0.08790510692499254
1102	52-53	-0.0053751163576905014
1102	54-55	-0.07773478919227017
1102	56-57	0.001520464402410937
1102	58-59	-0.0862262025831555
1102	60-61	-0.46347908785746483
1102	62-63	-0.47219822717143956
1102	64-65	-0.1899267417857473
1102	66-67	-0.10742054210526675
1102	68-69	0.09604137765894194
1102	70-71	0.050544290386703494
1102	72-73	0.0020620911538387077
1102	74-75	0.16981146387323776
1102	76-77	0.34003521375063883
1102	78-79	0.2201343888135341
1102	80-81	0.1567859082476062
1102	82-83	-0.103847801184763
1102	84-85	-0.04521537242133178
1102	86-87	-0.03625346859986678
1102	88-89	-0.21988814853114036
1102	90-91	-0.307984283215319
1102	92-93	-0.01296115655586405
1102	94-95	-0.13156552663909693
1102	96-97	-0.18814786564735542
1102	98-99	-0.180518163718137
1102	100-101	-0.051168270645529645
1103	1	-0.11714806974061176
1103	2	-0.08189601184088247
1103	3	-0.08855284955432552
1103	4	0.0737890998984625
1103	5	-0.11955854137774935
1103	6	0.05857572787348175
1103	7	0.015236619818622898
1103	8	0.05091817027025769
1103	9	0.23530384340479316
1103	10-11	0.12062651901522514
1103	12-13	0.17816566407399392
1103	14-15	0.19614685122610354
1103	16-17	0.3824900089269363
1103	18-19	0.3228936229548296
1103	20-21	0.017201834783016068
1103	22-23	0.31520225751387443
1103	24-25	0.28738515990266933
1103	26-27	0.2460036595972639
1103	28-29	0.16341653693908853
1103	30-31	0.20116182676196104
1103	32-33	0.13697167577845448
1103	34-35	0.267465903808187
1103	36-37	0.30613399668557406
1103	38-39	0.20303364989735684
1103	40-41	-0.059720507704412285
1103	42-43	-0.009612174412332308
1103	44-45	0.1756951509622482
1103	46-47	0.4156958850405701
1103	48-49	0.2779885170048644
1103	50-51	0.20046089136391743
1103	52-53	0.16974313635401472
1103	54-55	0.2717956418278007
1103	56-57	0.2739697952051543
1103	58-59	0.25205129055769504
1103	60-61	0.2365612311544254
1103	62-63	0.2963460598212535
1103	64-65	0.5200305790650788
1103	66-67	0.12945856969640346
1103	68-69	0.21722951010197988
1103	70-71	0.20144458442047863
1103	72-73	0.24858138035916255
1103	74-75	-0.048009047686520034
1103	76-77	-0.4246753252457083
1103	78-79	-0.22336392467170185
1103	80-81	-0.007610929168443192
1103	82-83	-0.12006243600269073
1103	84-85	-0.3638894669645012
1103	86-87	-0.31523792119552496
1103	88-89	-0.16946676568737473
1103	90-91	-0.1645784084402706
1103	92-93	-0.3171390912390635
1103	94-95	-0.21540006134786793
1103	96-97	-0.10103913215602844
1103	98-99	0.041775791380253224
1103	100-101	-0.2578218909977785
1104	1	-0.02808995691835392
1104	2	0.25867046291261175
1104	3	0.0906269343306434
1104	4	-0.03676517938720281
1104	5	-0.021118426797073653
1104	6	0.19259352002111285
1104	7	0.10733509113091344
1104	8	0.1481453557642638
1104	9	0.37525487245648037
1104	10-11	0.21380083857430066
1104	12-13	0.3012070624876415
1104	14-15	0.3197535899311106
1104	16-17	0.25316227227340704
1104	18-19	0.3189579847726165
1104	20-21	0.29116734713447556
1104	22-23	0.32342852598433325
1104	24-25	0.13762292425597877
1104	26-27	0.2718996483282794
1104	28-29	0.2647481118561288
1104	30-31	0.40544583935449197
1104	32-33	0.1581289216825752
1104	34-35	0.33086721033040334
1104	36-37	0.3827494234796802
1104	38-39	0.39223215240735243
1104	40-41	0.22648868711109316
1104	42-43	0.2561199790803954
1104	44-45	0.32453374536367363
1104	46-47	0.4542089833511582
1104	48-49	0.12841913991911014
1104	50-51	0.13162976550479755
1104	52-53	0.24700757002106855
1104	54-55	0.06133495566729863
1104	56-57	0.03822572986013739
1104	58-59	-0.07632160714217662
1104	60-61	0.2072902176070528
1104	62-63	0.22184687757937382
1104	64-65	0.3697460181491934
1104	66-67	0.30172664309609587
1104	68-69	0.12075674191986252
1104	70-71	0.09686041205786466
1104	72-73	0.023611869028719923
1104	74-75	-0.04095978366882491
1104	76-77	7.664059434873138E-4
1104	78-79	-0.15614798926041118
1104	80-81	-0.06980356083695227
1104	82-83	-0.03177244857631223
1104	84-85	-0.0060096821341417694
1104	86-87	0.05632536761017093
1104	88-89	-0.004582149939139413
1104	90-91	-0.09446014076393539
1104	92-93	-0.21039518660951373
1104	94-95	-0.10276451675877851
1104	96-97	0.0589984941710604
1104	98-99	0.10570036855676435
1104	100-101	-0.06372865711896125
1105	1	0.1318793620433354
1105	2	0.24363406282110844
1105	3	0.19369907514045792
1105	4	0.2333275365575176
1105	5	0.005339931767373685
1105	6	0.1334248685065731
1105	7	0.21462991168721146
1105	8	0.2789488490965226
1105	9	0.43684032856779
1105	10-11	0.3025026807822613
1105	12-13	0.26802578868628046
1105	14-15	0.3602646714377187
1105	16-17	0.4323449186475372
1105	18-19	0.31707002438884047
1105	20-21	0.23836366540987797
1105	22-23	0.28254604243502257
1105	24-25	0.26565705018279573
1105	26-27	0.1705539887532339
1105	28-29	0.2856435137373623
1105	30-31	0.31268010522990863
1105	32-33	0.3209496932837794
1105	34-35	0.17748085800980817
1105	36-37	0.33033463737226754
1105	38-39	0.3134989563312942
1105	40-41	0.3528541951648734
1105	42-43	0.33809890605144943
1105	44-45	0.3596917930807635
1105	46-47	0.531070366958339
1105	48-49	0.5798071877707613
1105	50-51	0.5131377116245801
1105	52-53	0.49220423816796455
1105	54-55	0.3948243855113347
1105	56-57	0.5499693009715116
1105	58-59	0.5626271635978028
1105	60-61	0.5919106743239126
1105	62-63	0.5997982454423223
1105	64-65	0.5063923653562696
1105	66-67	0.33093418417235654
1105	68-69	0.44373387916368046
1105	70-71	0.4947840593335755
1105	72-73	0.4022925843813496
1105	74-75	0.4489396955609237
1105	76-77	0.46545899001356617
1105	78-79	0.4841760455652562
1105	80-81	0.4975090224257386
1105	82-83	0.4085717711429879
1105	84-85	0.41971932894063
1105	86-87	0.6193910492540269
1105	88-89	0.6101958835139527
1105	90-91	0.645469615806249
1105	92-93	0.5571125172915536
1105	94-95	0.504282702435713
1105	96-97	0.5180339253876731
1105	98-99	0.30753584821712465
1105	100-101	0.4375039670517751
1106	1	0.10071242889235066
1106	2	0.14552844787921515
1106	3	0.24134110792976315
1106	4	0.1383242013135373
1106	5	0.18571461877399997
1106	6	0.056765375128456697
1106	7	0.08005784527730242
1106	8	0.24372515310987808
1106	9	0.19996929021751697
1106	10-11	0.3075847378400667
1106	12-13	0.3487026588531066
1106	14-15	0.35979832388182587
1106	16-17	0.3120955463102817
1106	18-19	0.415658746906594
1106	20-21	0.2456224300623262
1106	22-23	0.4027279556918657
1106	24-25	0.4496431024941785
1106	26-27	0.33601351498099064
1106	28-29	0.4222351625546068
1106	30-31	0.45139231557486426
1106	32-33	0.483417555945465
1106	34-35	0.4730928333792832
1106	36-37	0.3621975303528302
1106	38-39	0.3270657735029445
1106	40-41	0.3966791334041986
1106	42-43	0.33527945169065987
1106	44-45	0.5678864372487809
1106	46-47	0.6192192730318382
1106	48-49	0.36409860764613455
1106	50-51	0.20027632369381365
1106	52-53	0.27015324748317937
1106	54-55	0.46361002150381125
1106	56-57	0.4343016990957338
1106	58-59	0.3457772261126735
1106	60-61	0.41099799067599463
1106	62-63	0.5135654286528109
1106	64-65	0.5396203619640474
1106	66-67	0.3912664850884582
1106	68-69	0.24669763729647798
1106	70-71	0.3504631083029963
1106	72-73	0.47622396550756463
1106	74-75	0.4020711376936035
1106	76-77	0.18911726661423245
1106	78-79	0.32723940849678
1106	80-81	0.3616083054320782
1106	82-83	0.38908028690914165
1106	84-85	0.3155315027487475
1106	86-87	0.44207590389437
1106	88-89	0.367806189585437
1106	90-91	0.4791821445915829
1106	92-93	0.5311860233871748
1106	94-95	0.3043645751333024
1106	96-97	0.23108729934729055
1106	98-99	0.31915892207182495
1106	100-101	0.36011331780212075
1107	1	0.14918964592072825
1107	2	0.08177267927395349
1107	3	0.030067651081985503
1107	4	0.129169308022675
1107	5	0.24451655232353176
1107	6	0.06544686438669345
1107	7	0.033206143116068176
1107	8	0.10645811136981109
1107	9	0.1279877239908629
1107	10-11	0.18359273418165856
1107	12-13	0.13026453791287196
1107	14-15	0.08531616992568303
1107	16-17	-0.022818886715931797
1107	18-19	0.22004828113499997
1107	20-21	0.12883816382247204
1107	22-23	0.14392006433647708
1107	24-25	0.0766607053951418
1107	26-27	0.09081478190035597
1107	28-29	0.2465112891434842
1107	30-31	0.16743241965934175
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2316	3	-0.6192285429586484
2316	4	-0.26740709772040105
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2316	12-13	-0.6508460776089819
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2316	54-55	-1.2778386605041376
2316	56-57	-1.2398620767951982
2316	58-59	-1.5336459128664117
2316	60-61	-1.0523652084043391
2316	62-63	-0.6613370076660914
2316	64-65	-1.2672627935529661
2316	66-67	-1.5856812590980596
2316	68-69	-1.8521580692965074
2316	70-71	-1.3712774580201383
2316	72-73	-1.11268377504868
2316	74-75	-1.4181822757747256
2316	76-77	-1.1244390430080173
2316	78-79	-1.2043704678721312
2316	80-81	-1.3220685596224726
2316	82-83	-1.3400153708767988
2316	84-85	-1.7033938345644515
2316	86-87	-1.451390606804857
2316	88-89	-1.191932027142073
2316	90-91	-1.2486895553862318
2316	92-93	-1.451873946763886
2316	94-95	-1.2605014071590155
2316	96-97	-1.1292693248989245
2316	98-99	-0.7413734176413804
2316	100-101	-1.3631096114276566
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	6.0
7	177.0
8	641.0
9	581.0
10	444.0
11	560.0
12	759.0
13	1064.0
14	1377.0
15	1736.0
16	2315.0
17	2874.0
18	3683.0
19	4617.0
20	5651.0
21	6766.0
22	7628.0
23	8088.0
24	8345.0
25	9035.0
26	9822.0
27	11226.0
28	12794.0
29	15244.0
30	18423.0
31	22228.0
32	28290.0
33	37312.0
34	52487.0
35	79518.0
36	138017.0
37	251925.0
38	319396.0
39	104616.0
40	145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.19921979524357	18.153698960386627	12.672648695342431	23.97443254902737
2	15.374043715142498	19.158357592306572	41.086836380638545	24.38076231191238
3	19.78701845571221	23.586951019564204	29.2060294436861	27.420001081037487
4	12.230819667629016	15.56989162412762	38.083116776118175	34.11617193212519
5	13.364871384154748	37.852184915643875	35.17591433736615	13.607029362835233
6	32.847614046648275	36.57506555194047	17.06735719286685	13.509963208544404
7	28.84032494545779	31.69831101456661	21.068864312364934	18.39249972761067
8	25.86703156702685	36.51171270102049	19.18712439356048	18.434131338392177
9	25.637211121949715	16.209600827737443	19.84047522836133	38.312712821951514
10-11	25.284060804993036	25.930112783454824	28.3114400408078	20.474386370744345
12-13	25.73369783192543	23.11243077179131	28.422353949981105	22.73151744630216
14-15	22.529261385696017	25.184761899858987	25.820333397255133	26.46564331718986
16-17	22.587382770065414	28.3726331712773	26.403148843716988	22.636835214940294
18-19	22.42620305120332	27.75666441517746	27.7972683168355	22.019864216783716
20-21	23.199182386544145	26.710561582614666	27.81833875811469	22.271917272726494
22-23	22.86513597039196	26.669455407688535	27.6256624687123	22.839746153207212
24-25	22.85935582651348	26.7164244286863	27.75886267320094	22.665357071599285
26-27	22.82951315774826	26.79362146137451	27.812981946255793	22.563883434621435
28-29	22.550182352835396	27.234667847243216	27.62052456304253	22.59462523687886
30-31	22.911377121419843	26.802441532774274	27.603697921974053	22.682483423831833
32-33	22.660005086526613	26.940051772962796	27.687402968510632	22.712540171999958
34-35	23.02942050418268	26.926778849982576	27.64480116733217	22.398999478502574
36-37	22.941947660154945	26.769516120607197	27.648097990136932	22.640438229100926
38-39	22.7850702737048	27.10557796729038	27.622280014146366	22.487071744858454
40-41	22.94241863484134	27.111015584124214	27.441639813973563	22.50492596706089
42-43	22.724400170920994	27.02940851573612	27.37356256384918	22.8726287494937
44-45	22.924642571555363	26.956139579854092	27.339711913423958	22.779505935166586
46-47	23.062325158322892	27.026061063723894	27.21789349031184	22.693720287641376
48-49	22.899057753521213	26.94868639150624	27.376614049191538	22.775641805781007
50-51	22.697370534997056	27.243356718256308	27.387993359395917	22.671279387350722
52-53	22.845346725297215	27.373360977609934	27.060241985966204	22.72105031112665
54-55	22.735928450591246	27.13093624074223	27.185912938849455	22.947222369817062
56-57	22.951640376466635	27.177631798897757	27.065933095648553	22.80479472898706
58-59	23.175658504579737	27.02629732784042	27.056767481018294	22.741276686561548
60-61	22.937729368075008	27.215236278863557	27.331091409354936	22.5159429437065
62-63	22.62465132309446	27.31043114256148	27.29635315825414	22.768564376089916
64-65	22.66851196356053	27.407889501701238	27.198621515201403	22.724977019536823
66-67	22.79262734038655	27.152405586283308	26.968281962870975	23.086685110459175
68-69	22.76029138714063	27.171602269239937	27.03950425096738	23.028602092652058
70-71	22.828430868387674	26.940844732421404	27.156313209228976	23.074411189961946
72-73	22.805632156525345	26.996457407480406	27.28220425693822	22.915706179056027
74-75	22.633411035942853	27.460376599695508	27.023701368163312	22.88251099619833
76-77	22.74047274150253	27.032201481522165	26.992940800900723	23.234384976074583
78-79	22.725514894843034	27.05840033818981	27.03842107203769	23.177663694929468
80-81	22.878181501065555	27.05014593776208	26.944254817488876	23.12741774368349
82-83	22.849181977361567	27.062969187771102	27.177044113828092	22.910804721039238
84-85	22.682640940970757	27.18345201181999	27.1723948768608	22.96151217034845
86-87	22.662174593564647	27.182483828493986	27.07202441932757	23.0833171586138
88-89	22.6427481982509	27.112654477727393	26.91569095583449	23.32890636818722
90-91	22.68718312732859	26.91083758116313	27.06540543413592	23.336573857372365
92-93	22.872740996148867	27.012968633404522	27.120440178747568	22.993850191699046
94-95	22.671191561950582	26.935702444120817	27.276055624315937	23.117050369612667
96-97	22.483589488873164	26.9006386781937	27.441348788234233	23.1744230446989
98-99	22.630433702022046	26.95112774866777	27.116103520797232	23.302335028512957
100-101	22.643097823409327	26.772086686616785	27.15603719547331	23.428778294500578
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25.0
1	19.5
2	34.0
3	54.0
4	52.5
5	53.0
6	82.0
7	151.0
8	264.5
9	336.0
10	323.5
11	321.5
12	364.0
13	398.0
14	434.0
15	497.0
16	573.5
17	622.0
18	694.5
19	826.0
20	943.5
21	1041.5
22	1226.5
23	1572.0
24	2004.0
25	2603.0
26	3569.5
27	4821.5
28	6629.5
29	9429.0
30	12625.5
31	16150.0
32	20109.5
33	23559.5
34	26531.5
35	28610.0
36	30362.0
37	31972.5
38	32387.0
39	32780.0
40	33893.5
41	34893.5
42	35522.5
43	37052.0
44	39264.5
45	41366.5
46	42967.5
47	45622.0
48	49862.0
49	49644.5
50	45766.0
51	43846.0
52	42901.5
53	41760.0
54	40376.5
55	38004.5
56	34675.0
57	31018.5
58	27549.5
59	24211.5
60	20712.5
61	17187.0
62	13678.5
63	10415.0
64	7790.5
65	5774.5
66	4214.0
67	3159.5
68	2408.5
69	1817.0
70	1357.0
71	985.0
72	724.0
73	560.0
74	455.5
75	355.0
76	239.5
77	168.0
78	118.5
79	87.5
80	76.5
81	58.5
82	41.0
83	31.0
84	30.0
85	19.0
86	14.0
87	14.0
88	12.5
89	11.5
90	8.0
91	6.5
92	6.0
93	5.5
94	5.0
95	4.0
96	1.0
97	1.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.47371490275229045
2	0.3685591000444429
3	0.19215767204919373
4	0.22152936612801435
5	0.24396488755265283
6	0.19720994595779553
7	0.18667723933477823
8	0.18633471229012724
9	0.18873240160268404
10-11	0.19930792410628273
12-13	0.1859493693648949
14-15	0.19733839359953967
16-17	0.08678778993843933
18-19	0.035579996763119424
20-21	1.712635223254846E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.1411639582767807
46-47	0.2098834466098814
48-49	0.08605991996855601
50-51	0.06238273800705777
52-53	0.07043212355635554
54-55	0.07989443316483857
56-57	0.1078960190650553
58-59	0.09209695913052934
60-61	0.14381854287282572
62-63	0.24417896695555968
64-65	0.13555507792062108
66-67	0.1013451893361055
68-69	0.10558396151366127
70-71	0.08828634575878733
72-73	0.07270136522716822
74-75	0.13636857965166713
76-77	0.214336298190344
78-79	0.13546944615945833
80-81	0.08790100283355498
82-83	0.0868306058190207
84-85	0.09620728366634097
86-87	0.07248728582426135
88-89	0.09226822265285484
90-91	0.11243450240668064
92-93	0.1627859779703731
94-95	0.12866172114702032
96-97	0.09286764498099404
98-99	0.08160706838809342
100-101	0.061098261589616634
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1167791.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.56313434950756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.69426584597815	52.492681215381786
2	13.638222937616499	18.428821772454384
3	4.368760620957677	8.85501482323805
4	1.8209733995909743	4.9212268177377805
5	0.8731703735851427	2.949706363027136
6	0.4817355366920719	1.9528537671875155
7	0.29737449036781705	1.4064086851386015
8	0.19527969765259265	1.0554966758586668
9	0.1298953434016557	0.7898522883859336
>10	0.4827414669269096	5.1639286884234625
>50	0.012112117819276142	0.5401650940252716
>100	0.004323674666242773	0.5838966391131575
>500	6.358304138627103E-4	0.31660558712702946
>1k	5.086643310901682E-4	0.543341582901312
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	2382	0.20397485508965216	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1496	0.12810511469946248	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1267	0.1084954413931945	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	1179	0.10095984641087317	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.56317611627423E-5	0.0
2	0.0	0.0	0.0	8.56317611627423E-5	0.0
3	0.0	0.0	0.0	8.56317611627423E-5	0.0
4	0.0	0.0	0.0	8.56317611627423E-5	0.0
5	0.0	0.0	0.0	8.56317611627423E-5	0.0
6	0.0	0.0	0.0	8.56317611627423E-5	0.0
7	0.0	0.0	0.0	8.56317611627423E-5	0.0
8	0.0	0.0	0.0	8.56317611627423E-5	0.0
9	0.0	0.0	0.0	8.56317611627423E-5	8.56317611627423E-5
10-11	0.0	0.0	0.0	2.568952834882269E-4	1.712635223254846E-4
12-13	0.0	0.0	0.0	3.425270446509692E-4	1.712635223254846E-4
14-15	0.0	0.0	0.0	5.56606447557825E-4	1.712635223254846E-4
16-17	0.0	0.0	0.0	0.0010703970145342788	1.712635223254846E-4
18-19	0.0	0.0	0.0	0.001370108178603877	1.712635223254846E-4
20-21	4.281588058137115E-5	0.0	0.0	0.002269241670812671	1.712635223254846E-4
22-23	8.56317611627423E-5	0.0	0.0	0.003981876894067517	2.568952834882269E-4
24-25	8.56317611627423E-5	0.0	0.0	0.005822959759066477	3.425270446509692E-4
26-27	8.56317611627423E-5	0.0	0.0	0.006893356773600756	3.425270446509692E-4
28-29	8.56317611627423E-5	0.0	0.0	0.008306280832786003	3.425270446509692E-4
30-31	8.56317611627423E-5	0.0	0.0	0.011688735398714324	3.425270446509692E-4
32-33	8.56317611627423E-5	0.0	0.0	0.017897038083013142	3.425270446509692E-4
34-35	8.56317611627423E-5	0.0	0.0	0.027273715930333425	3.425270446509692E-4
36-37	8.56317611627423E-5	0.0	0.0	0.041531404163930016	3.425270446509692E-4
38-39	8.56317611627423E-5	0.0	0.0	0.06508013848368416	3.425270446509692E-4
40-41	8.56317611627423E-5	0.0	0.0	0.09385241023436557	3.425270446509692E-4
42-43	8.56317611627423E-5	0.0	0.0	0.12056951971714117	3.425270446509692E-4
44-45	8.56317611627423E-5	0.0	0.0	0.1567489388083998	3.853429252323404E-4
46-47	8.56317611627423E-5	0.0	0.0	0.20564467443232565	4.2815880581371154E-4
48-49	8.56317611627423E-5	0.0	0.0	0.250558533162184	4.2815880581371154E-4
50-51	8.56317611627423E-5	0.0	0.0	0.3008243769647137	4.2815880581371154E-4
52-53	8.56317611627423E-5	0.0	0.0	0.35177527485654536	4.709746863950827E-4
54-55	8.56317611627423E-5	0.0	0.0	0.41561375280336976	5.137905669764538E-4
56-57	8.56317611627423E-5	0.0	0.0	0.49071280734309475	5.56606447557825E-4
58-59	8.56317611627423E-5	0.0	0.0	0.5724483233729323	5.994223281391961E-4
60-61	8.56317611627423E-5	0.0	0.0	0.6549117093726531	5.994223281391961E-4
62-63	8.56317611627423E-5	0.0	0.0	0.7399868640878375	5.994223281391961E-4
64-65	8.56317611627423E-5	0.0	0.0	0.8364938589182482	5.994223281391961E-4
66-67	8.56317611627423E-5	0.0	0.0	0.9485858342802779	5.994223281391961E-4
68-69	8.56317611627423E-5	0.0	0.0	1.0781038730389256	5.994223281391961E-4
70-71	8.56317611627423E-5	0.0	0.0	1.2172983008089633	5.994223281391961E-4
72-73	8.56317611627423E-5	0.0	0.0	1.3586335226080695	0.00111321289511565
74-75	8.56317611627423E-5	0.0	0.0	1.5019382749139187	0.00111321289511565
76-77	8.56317611627423E-5	0.0	0.0	1.660271401303829	0.00111321289511565
78-79	8.56317611627423E-5	0.0	0.0	1.8609494335887158	0.00111321289511565
80-81	8.56317611627423E-5	0.0	0.0	2.079267608673127	0.00111321289511565
82-83	8.56317611627423E-5	0.0	0.0	2.3005400795176536	0.0011988446562783922
84-85	8.56317611627423E-5	0.0	0.0	2.520485258064157	0.0011988446562783922
86-87	8.56317611627423E-5	0.0	0.0	2.763765091527508	0.0011988446562783922
88-89	8.56317611627423E-5	0.0	0.0	3.0482766179907195	0.0011988446562783922
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATACG	100	1.26747E-6	33.355415	1
TACATGG	3135	0.0	24.909046	2
GTACATG	3235	0.0	24.893202	1
GTATAGT	345	0.0	23.480005	1
ACATGGG	3125	0.0	22.825146	3
CCATGCG	175	4.0272644E-6	21.72797	9
CTGATCG	155	3.8875467E-5	21.465132	9
CATGGGG	2030	0.0	21.082338	4
TCTAGAC	540	0.0	20.253794	3
AGAGTAC	3500	0.0	19.419373	10-11
ACACCGT	270	2.282286E-8	19.364048	6
GAGTACT	2105	0.0	18.74102	12-13
TACACTG	1175	0.0	18.616253	5
TATACTG	645	0.0	18.431158	5
GTAGGAC	340	8.785719E-10	18.181793	3
GCACCGT	210	1.9452638E-5	18.106642	6
GTATCAA	5490	0.0	17.879822	1
GTACCTG	1000	0.0	17.63072	1
TACGACT	135	0.009341001	17.611994	4
GTATAGG	390	2.8740033E-10	17.105343	1
>>END_MODULE