FastQCFastQC Report
Fri 10 Feb 2017
SRR2031621_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031621_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167791
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25410.21759030511452818No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17120.1466015751106148No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15800.13529818263713284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG31750.028.1375391
TACATGG31350.028.031012
ACATGGG30500.026.7852693
CATGGGG20500.023.6327194
GTACCGT1452.3354743E-522.9296516
CCGTAGA1503.0326762E-522.1672272
TTGTACG1100.00287840221.5896059
TACCGTA1100.00287840221.5896057
GTATAGA4200.021.4969231
CAGTACG1856.5556724E-620.5392999
CCGACGT704.8557768E-420.35591512-13
TAGCGCA1200.00476520819.7904729
GGACCGT1707.957687E-519.5576426
AGAGTAC35200.019.22824110-11
CTTACGC1250.00603330318.9988523
GTATAGC4550.018.7989271
GAGTACT23200.018.6303412-13
CAGTGCG2552.6766065E-718.6263269
GTACTAT2854.2522515E-818.3408641
GCGTAAG1300.00754530318.2767371
Produced by FastQC (version 0.11.5)