##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031621_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1167791 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.43019855436461 34.0 31.0 34.0 31.0 34.0 2 32.65506584654275 34.0 31.0 34.0 31.0 34.0 3 32.76731024643964 34.0 31.0 34.0 31.0 34.0 4 36.222126219503316 37.0 37.0 37.0 35.0 37.0 5 35.8721646253482 37.0 35.0 37.0 35.0 37.0 6 35.93516219940041 37.0 36.0 37.0 35.0 37.0 7 35.911229834790646 37.0 36.0 37.0 35.0 37.0 8 36.02691663148629 37.0 36.0 37.0 35.0 37.0 9 37.9386157283281 39.0 38.0 39.0 35.0 39.0 10-11 37.90823143867353 39.0 38.0 39.0 35.0 39.0 12-13 37.919541253529104 39.0 38.0 39.0 35.0 39.0 14-15 39.37935469617423 41.0 39.0 41.0 36.0 41.0 16-17 39.34978818983876 41.0 39.0 41.0 36.0 41.0 18-19 39.35010245840223 41.0 39.0 41.0 36.0 41.0 20-21 39.36371619579188 41.0 39.0 41.0 36.0 41.0 22-23 39.29957929115741 41.0 39.0 41.0 36.0 41.0 24-25 39.228452694018024 41.0 39.0 41.0 36.0 41.0 26-27 39.11069831844911 41.0 39.0 41.0 36.0 41.0 28-29 39.02588819403472 40.0 39.0 41.0 35.0 41.0 30-31 38.92254821282233 40.0 38.5 41.0 35.0 41.0 32-33 38.77235096006049 40.0 38.0 41.0 35.0 41.0 34-35 38.644296796258914 40.0 38.0 41.0 35.0 41.0 36-37 38.51245556781993 40.0 38.0 41.0 34.0 41.0 38-39 38.396502456347065 40.0 38.0 41.0 34.0 41.0 40-41 38.27719086720141 40.0 38.0 41.0 34.0 41.0 42-43 38.11800227951748 40.0 38.0 41.0 33.0 41.0 44-45 38.04337419966416 40.0 38.0 41.0 33.0 41.0 46-47 38.04682687227424 40.0 38.0 41.0 33.0 41.0 48-49 38.15828174733321 40.0 38.0 41.0 33.0 41.0 50-51 38.068884329473335 40.0 38.0 41.0 33.0 41.0 52-53 37.96777676827446 40.0 37.5 41.0 33.0 41.0 54-55 37.847394782114264 40.0 37.0 41.0 33.0 41.0 56-57 37.710799706454324 40.0 37.0 41.0 33.0 41.0 58-59 37.49249137902245 40.0 36.5 41.0 32.5 41.0 60-61 37.182500550184066 39.0 36.0 41.0 32.0 41.0 62-63 36.9809700537168 39.0 35.0 41.0 32.0 41.0 64-65 36.67153283421435 39.0 35.0 41.0 31.0 41.0 66-67 36.36330345070308 38.0 35.0 40.0 31.0 41.0 68-69 36.04703881088311 37.0 35.0 40.0 31.0 41.0 70-71 35.69162547065356 37.0 35.0 39.0 30.5 41.0 72-73 35.31215089001371 36.0 35.0 39.0 30.0 41.0 74-75 34.95429019405013 36.0 34.0 39.0 30.0 40.5 76-77 34.349840425213074 35.0 34.0 37.0 29.0 39.0 78-79 34.31979352469749 35.0 34.0 37.0 30.0 39.0 80-81 34.059305988828484 35.0 34.0 36.5 29.0 39.0 82-83 33.79729377945198 35.0 34.0 36.0 29.0 38.0 84-85 33.54657682753164 35.0 34.0 36.0 29.0 37.0 86-87 33.322739257281484 35.0 34.0 35.5 29.0 37.0 88-89 33.11788153873424 35.0 34.0 35.0 29.0 36.0 90-91 32.96826444115428 35.0 34.0 35.0 29.0 36.0 92-93 32.84541369131976 35.0 34.0 35.0 29.0 36.0 94-95 32.730672697426165 35.0 33.5 35.0 28.5 36.0 96-97 32.63311071929823 35.0 33.0 35.0 27.0 35.0 98-99 32.54652587663375 35.0 33.0 35.0 27.0 35.0 100-101 32.10391671112382 34.5 32.5 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1051727025243494 1101 2 0.18433593982992846 1101 3 0.09959850909481105 1101 4 0.09843307639534515 1101 5 0.17974889080919354 1101 6 0.0634880862842877 1101 7 0.14761996860259785 1101 8 0.045696631200932813 1101 9 0.05707280756156763 1101 10-11 0.03519705643324045 1101 12-13 -0.012666171263639114 1101 14-15 0.026827350272071726 1101 16-17 0.12040016620821348 1101 18-19 0.0651504423598297 1101 20-21 -0.07181784337729624 1101 22-23 0.06237275103211459 1101 24-25 0.13118132520737902 1101 26-27 0.07250450221044247 1101 28-29 -0.14303589594828736 1101 30-31 -0.19882664189363908 1101 32-33 -0.15649853466971564 1101 34-35 -0.034011411692397076 1101 36-37 -0.09139684662545022 1101 38-39 -0.01025664460929221 1101 40-41 -0.03349453477770936 1101 42-43 -0.13434779381081086 1101 44-45 -0.002395703322811471 1101 46-47 0.1849938321984368 1101 48-49 0.060233242965530565 1101 50-51 0.09884616323599005 1101 52-53 -0.05873989675502145 1101 54-55 0.08280795588755296 1101 56-57 0.05882140471799602 1101 58-59 0.08567492468137772 1101 60-61 -0.07828491789723557 1101 62-63 -0.10786059277026538 1101 64-65 -0.1006327727620473 1101 66-67 0.12643886371673574 1101 68-69 0.11157887008140221 1101 70-71 0.10334918125563064 1101 72-73 -0.06349646669012543 1101 74-75 -0.06907250408713139 1101 76-77 -0.1674732659691216 1101 78-79 0.0810378576218298 1101 80-81 0.12764309172591481 1101 82-83 0.07888112737398245 1101 84-85 0.026273639513782143 1101 86-87 -0.10916611906562679 1101 88-89 -0.14765372207182992 1101 90-91 -0.1136012936403219 1101 92-93 -0.34836687542861 1101 94-95 -0.05806569193366329 1101 96-97 -0.07701148457344686 1101 98-99 -0.29384810084467716 1101 100-101 -0.049853887804751196 1102 1 0.07808116721757585 1102 2 0.1652178776087112 1102 3 0.19269832892943128 1102 4 0.1722782984196627 1102 5 -0.10081429283117416 1102 6 0.06818470523194264 1102 7 0.10043614345028118 1102 8 0.12659756077218276 1102 9 0.17538654170726886 1102 10-11 0.15077340600541334 1102 12-13 0.04157746776911608 1102 14-15 0.05354931556328779 1102 16-17 0.17866031270166616 1102 18-19 0.22112490096542103 1102 20-21 0.14796133853840843 1102 22-23 0.12333640484766306 1102 24-25 0.10776393180523058 1102 26-27 0.10387637690568141 1102 28-29 -0.31955382779341335 1102 30-31 -0.24129639618090692 1102 32-33 0.08088398569513089 1102 34-35 0.11957470068768572 1102 36-37 0.11290915734742413 1102 38-39 -0.006907929635026733 1102 40-41 0.07043265992922443 1102 42-43 0.1861417838014532 1102 44-45 0.28451895166817565 1102 46-47 0.3088326380766446 1102 48-49 0.2512347263785131 1102 50-51 0.3428973692148105 1102 52-53 0.14821982524620125 1102 54-55 0.07233731873857607 1102 56-57 0.07851845996358264 1102 58-59 0.23859427115839793 1102 60-61 0.4611405033869147 1102 62-63 0.17047901127652665 1102 64-65 0.3006016937482343 1102 66-67 0.29071427550499607 1102 68-69 0.23926069082967416 1102 70-71 0.18203063756680393 1102 72-73 0.3344842438082338 1102 74-75 0.25135496523085266 1102 76-77 0.19700027641080453 1102 78-79 0.18368849590005 1102 80-81 0.25613437346658685 1102 82-83 -0.014589289059564692 1102 84-85 -0.06253198205678956 1102 86-87 0.012166796790474166 1102 88-89 0.11278072801311367 1102 90-91 0.1497270949830849 1102 92-93 0.2800315005164933 1102 94-95 0.23551251575442222 1102 96-97 0.2795470999733922 1102 98-99 0.26897161571564965 1102 100-101 0.1574897763792933 1103 1 0.26361745924523206 1103 2 0.23258897969448356 1103 3 0.16651850613640562 1103 4 0.16022783081746894 1103 5 0.15517111365330294 1103 6 0.1105737541949523 1103 7 0.13142867951612658 1103 8 0.033914969734802014 1103 9 0.18158681902340135 1103 10-11 0.18034346766400233 1103 12-13 0.11024320349274319 1103 14-15 0.10469545723052676 1103 16-17 0.21587230219905962 1103 18-19 0.17656600078939277 1103 20-21 0.17593486566523353 1103 22-23 0.1401204227847117 1103 24-25 0.24426148807975068 1103 26-27 0.1157252494484311 1103 28-29 0.26612997281431916 1103 30-31 0.10553561121418653 1103 32-33 0.08059241040182741 1103 34-35 0.0033040126093268896 1103 36-37 0.12201633708171045 1103 38-39 0.22856886726531656 1103 40-41 0.23720782735146173 1103 42-43 0.14388401747471136 1103 44-45 0.07482075422301904 1103 46-47 0.22623914222163677 1103 48-49 -0.11781846521546413 1103 50-51 0.2051469734001259 1103 52-53 0.3596964844718755 1103 54-55 0.20239661718776603 1103 56-57 0.30489151899652 1103 58-59 0.384302263173268 1103 60-61 0.2449976560779632 1103 62-63 0.09521915032794226 1103 64-65 0.1786184900585539 1103 66-67 0.051281298486834714 1103 68-69 0.08781215657327124 1103 70-71 0.03744813601796437 1103 72-73 0.15914325314061273 1103 74-75 0.06763794569088333 1103 76-77 -0.041097256262339954 1103 78-79 0.03183332965787855 1103 80-81 0.00958410745945315 1103 82-83 -0.034935639205592395 1103 84-85 0.025934322066568427 1103 86-87 0.03810814737903456 1103 88-89 0.03728927859381059 1103 90-91 0.1389063572435063 1103 92-93 -0.11402095353458463 1103 94-95 -0.11966405201593489 1103 96-97 -0.04534202274390964 1103 98-99 -0.15889570523351182 1103 100-101 -0.08185617057662142 1104 1 0.21015215015157196 1104 2 0.22483965800829253 1104 3 0.16889331351418946 1104 4 0.21501856982499845 1104 5 0.12171866179703983 1104 6 0.08943588868543628 1104 7 0.15407632503453073 1104 8 0.17104686041047046 1104 9 0.23101249757355902 1104 10-11 0.19292134376182446 1104 12-13 0.13313428567507657 1104 14-15 0.11673966343792586 1104 16-17 0.2872030262695091 1104 18-19 0.2143591883443392 1104 20-21 0.14642466968690115 1104 22-23 0.19213692920860126 1104 24-25 0.10697739040256238 1104 26-27 0.10584030281541601 1104 28-29 0.06447743965493657 1104 30-31 0.10320145539581205 1104 32-33 0.057447965012002555 1104 34-35 0.11706456547053534 1104 36-37 0.26971007012387105 1104 38-39 0.2638128238910511 1104 40-41 -0.030183949688911582 1104 42-43 0.09063283810679934 1104 44-45 0.1325610947507343 1104 46-47 0.30361087709784584 1104 48-49 0.2915498284589617 1104 50-51 0.37726000364298784 1104 52-53 0.42627192147614323 1104 54-55 0.27487172992494635 1104 56-57 0.36664265582209765 1104 58-59 0.14924171125744579 1104 60-61 0.21306680154437174 1104 62-63 0.19920962084611205 1104 64-65 0.24434213508535407 1104 66-67 0.35768439178689704 1104 68-69 0.29409282250253455 1104 70-71 0.3729222939161829 1104 72-73 0.1625594022089274 1104 74-75 0.11487789388377934 1104 76-77 0.15477708314981697 1104 78-79 0.1286038941473251 1104 80-81 0.10684201796929216 1104 82-83 0.09191424309396012 1104 84-85 0.115872115726809 1104 86-87 0.14014970650814718 1104 88-89 0.07912209669225945 1104 90-91 0.10310509609977458 1104 92-93 0.23993973735284868 1104 94-95 0.15293537886223163 1104 96-97 0.12343805231966343 1104 98-99 0.23491485849962146 1104 100-101 0.272681677915962 1105 1 0.15451720068109154 1105 2 0.19001129688708573 1105 3 0.09589397288738155 1105 4 0.10787177182196217 1105 5 0.11093992860096336 1105 6 0.07648526093525021 1105 7 0.14037212792231202 1105 8 0.10492653153126952 1105 9 0.13441567063883042 1105 10-11 0.22930394169220136 1105 12-13 0.10382043749238079 1105 14-15 0.16202110813804182 1105 16-17 0.17448250630664575 1105 18-19 0.033353173246048584 1105 20-21 0.0028495213279384757 1105 22-23 0.06669992626160592 1105 24-25 0.19145117022573999 1105 26-27 0.33376677597623683 1105 28-29 0.23427007351442342 1105 30-31 0.07315422274473349 1105 32-33 0.10662643984144182 1105 34-35 0.16110127506373573 1105 36-37 0.16654642446294332 1105 38-39 0.17651988725815926 1105 40-41 0.21429682756405555 1105 42-43 0.19782135155666225 1105 44-45 0.1273076397697821 1105 46-47 0.18937649011305524 1105 48-49 0.27956672994322673 1105 50-51 0.462381045721763 1105 52-53 0.37960983005202564 1105 54-55 0.1835512341839589 1105 56-57 0.010925621117543471 1105 58-59 0.13918963423188302 1105 60-61 0.107960023457089 1105 62-63 0.17283200185615755 1105 64-65 0.10928904881512125 1105 66-67 0.04760271025068619 1105 68-69 0.10445574688992565 1105 70-71 0.026760787853987722 1105 72-73 0.21163695257023818 1105 74-75 0.007116129454949771 1105 76-77 -0.05890692906877604 1105 78-79 -0.09546726433101327 1105 80-81 -0.036169299027726254 1105 82-83 0.013055900677422017 1105 84-85 -0.03868899740278664 1105 86-87 0.12186757552164096 1105 88-89 0.25322622383017546 1105 90-91 0.11226634648665623 1105 92-93 0.1678312233647148 1105 94-95 -0.0021922569484615906 1105 96-97 -0.024607377147589204 1105 98-99 0.06313603110737631 1105 100-101 0.17084485259603355 1106 1 0.2611218217537967 1106 2 0.18609029021325085 1106 3 0.21076882581820655 1106 4 0.17015033005800007 1106 5 0.06300208145905373 1106 6 0.06562225398653965 1106 7 0.18765382028209387 1106 8 0.18457517447245664 1106 9 0.26624244577634926 1106 10-11 0.25046681891835476 1106 12-13 0.136557784515297 1106 14-15 0.10240823107950092 1106 16-17 0.15760013818285756 1106 18-19 0.05802057064958177 1106 20-21 0.19768559157252952 1106 22-23 0.18184419651358041 1106 24-25 0.1773941224697424 1106 26-27 0.22278862769028507 1106 28-29 0.23422272645798614 1106 30-31 0.08066313069534914 1106 32-33 0.269422798894702 1106 34-35 0.030888489850099177 1106 36-37 0.08940172850646633 1106 38-39 0.04546449204013214 1106 40-41 0.03507355392991229 1106 42-43 0.23664384283307527 1106 44-45 0.25730254472370717 1106 46-47 0.13892715397745548 1106 48-49 0.29316958115782654 1106 50-51 0.34471104045911716 1106 52-53 0.17404525201905585 1106 54-55 0.07437730204862447 1106 56-57 0.04162913971613591 1106 58-59 0.3172556284865564 1106 60-61 0.0572605440233076 1106 62-63 0.01230411150691424 1106 64-65 0.13283687688412726 1106 66-67 0.2564631462123259 1106 68-69 0.20375509425474547 1106 70-71 0.21025116257451515 1106 72-73 0.3251437688703618 1106 74-75 0.349388000443021 1106 76-77 0.0955852590859152 1106 78-79 0.17276973240749527 1106 80-81 0.3420213897755744 1106 82-83 0.28694772815648406 1106 84-85 0.1941072610903518 1106 86-87 0.0420458853741863 1106 88-89 0.1456993826581865 1106 90-91 0.28142439012304266 1106 92-93 0.3140921467077362 1106 94-95 0.27825148134047595 1106 96-97 0.18616412009203742 1106 98-99 0.2298559108374718 1106 100-101 0.32052607701605496 1107 1 0.024454425121732015 1107 2 0.026040276848441124 1107 3 0.11788985300834298 1107 4 0.12071181300418488 1107 5 0.07243554242898398 1107 6 0.025217863888741476 1107 7 0.157064512772358 1107 8 0.07661168133036966 1107 9 0.11731742717833527 1107 10-11 0.06501320446245984 1107 12-13 0.04721807042557913 1107 14-15 0.0867931439828169 1107 16-17 0.13849651422668785 1107 18-19 0.005529080650639173 1107 20-21 0.023443118535631413 1107 22-23 0.08085817964493458 1107 24-25 0.1602489259104587 1107 26-27 0.10938742904415477 1107 28-29 0.08097065008213633 1107 30-31 0.12741379168975442 1107 32-33 0.05986945720970738 1107 34-35 0.10660978691274181 1107 36-37 0.057543569371866 1107 38-39 0.12071681316908922 1107 40-41 0.02871963449877768 1107 42-43 0.06062167657486128 1107 44-45 0.10109290080726652 1107 46-47 0.20334713742440869 1107 48-49 0.06556328262988131 1107 50-51 0.16703276519560717 1107 52-53 0.23677410422360623 1107 54-55 0.22841476215548084 1107 56-57 0.15388144796335013 1107 58-59 0.06041703349819727 1107 60-61 0.19320358417891725 1107 62-63 -0.06913386129328813 1107 64-65 -0.1788320494548472 1107 66-67 0.06909458024009041 1107 68-69 0.09803986771320439 1107 70-71 0.08979559975508522 1107 72-73 0.11807660346556759 1107 74-75 0.1056151152225624 1107 76-77 0.022394419292922407 1107 78-79 0.13648548710047237 1107 80-81 0.10369868924710346 1107 82-83 0.30666685786597014 1107 84-85 0.3608328348914611 1107 86-87 -0.012314664933846586 1107 88-89 -0.05734465357816276 1107 90-91 0.25162328422545244 1107 92-93 0.2224631054636177 1107 94-95 0.15021179269218976 1107 96-97 0.07124690138903134 1107 98-99 0.3253422288168437 1107 100-101 0.2752234043765469 1108 1 0.05769558137132691 1108 2 -0.016369994010183575 1108 3 0.0120737836798952 1108 4 0.040227698255421274 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-0.8910067552526542 2316 62-63 -0.980529873931637 2316 64-65 -1.01530991738003 2316 66-67 -1.213724468494327 2316 68-69 -1.1481031535317925 2316 70-71 -1.1457566929404805 2316 72-73 -1.1684704739144607 2316 74-75 -0.92391383688026 2316 76-77 -0.7931505475413516 2316 78-79 -0.8864579766863514 2316 80-81 -1.3067354926236376 2316 82-83 -1.52850313201737 2316 84-85 -1.4998175720177684 2316 86-87 -1.1339569785106178 2316 88-89 -0.9611391507101033 2316 90-91 -0.9237958970721607 2316 92-93 -0.7656529272087198 2316 94-95 -0.8308496722807241 2316 96-97 -0.7057242287371999 2316 98-99 -0.7545654130033626 2316 100-101 -0.9941823411918023 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 2.0 13 14.0 14 50.0 15 173.0 16 438.0 17 767.0 18 1336.0 19 1932.0 20 2950.0 21 3998.0 22 4816.0 23 5263.0 24 5257.0 25 5689.0 26 6712.0 27 7980.0 28 10198.0 29 12945.0 30 16158.0 31 20301.0 32 25991.0 33 34154.0 34 47916.0 35 73968.0 36 135146.0 37 272228.0 38 360750.0 39 110539.0 40 119.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.82133494172438 18.414820957975074 13.049386058402416 23.71445804189813 2 15.720146707097038 19.454446898316363 40.64990593173821 24.17550046284839 3 19.82453961987866 23.52573461724547 29.631139293619974 27.0185864692559 4 12.385723767315392 15.811848331085276 37.949600702250194 33.852827199349136 5 13.056188992722156 37.87287280001301 35.53829409543317 13.532644111831654 6 32.83729708483796 36.88065758342032 16.886497669531618 13.395547662210106 7 28.726972549026325 31.84645197642386 21.061217289737634 18.36535818481218 8 25.704942065831986 36.66837644749788 19.250191172906796 18.376490313763334 9 25.450444471656315 16.10339521369834 19.90416093290666 38.541999381738684 10-11 25.22882947376714 25.884169341945608 28.481295026250415 20.40570615803684 12-13 25.640632613198765 23.14921933804936 28.56076130060944 22.649386748142433 14-15 22.423961136881516 25.21996658648679 25.925443850826046 26.430628425805647 16-17 22.429270306073605 28.420667739347195 26.52700697299431 22.62305498158489 18-19 22.2493151599901 27.766612347586168 28.092184303526917 21.891888188896814 20-21 23.048816098086043 26.641068478863083 28.081180622217506 22.22893480083337 22-23 22.6439685775416 26.542574759358377 27.940845899019795 22.872610764080225 24-25 22.642193680204763 26.64877533736773 28.072617446101226 22.636413536326277 26-27 22.601261698368972 26.680245009595037 28.16544227520164 22.553051016834345 28-29 22.35441187240073 27.09092692210675 28.035711762038073 22.51894944345445 30-31 22.695966059001048 26.76138307772093 27.931777118946382 22.610873744331638 32-33 22.45761441901847 26.94664541857233 27.914455583233643 22.681284579175554 34-35 22.881705716176953 26.793878356657995 27.994007489353834 22.330408437811215 36-37 22.722173745130764 26.72832724348792 28.02586250450637 22.523636506874947 38-39 22.551552461013998 27.061263530888663 27.90156800317865 22.485616004918686 40-41 22.80887590330804 27.005131911446483 27.75950491140966 22.426487273835814 42-43 22.550139536954813 26.978928592530682 27.688858708450397 22.7820731620641 44-45 22.778134101050615 26.872788024569466 27.672717121471223 22.676360752908696 46-47 22.896091852052294 26.97229213104057 27.554759370469544 22.57685664643759 48-49 22.722267455481408 26.983468897482492 27.582746526378365 22.71151712065773 50-51 22.588908129550745 27.180332389570307 27.587940387062737 22.64281909381621 52-53 22.706588764599147 27.312764013423635 27.31798755085456 22.662659671122658 54-55 22.51329770722394 27.155710018141317 27.409183178459234 22.92180909617551 56-57 22.785284353107706 27.039256168269837 27.343334552158733 22.832124926463727 58-59 22.980182241514107 26.946474155050005 27.393386316558356 22.67995728687753 60-61 22.785080029337454 27.17375248385733 27.56894323082779 22.472224255977423 62-63 22.500858458405656 27.248454560790414 27.53656262122246 22.71412435958147 64-65 22.48728582426136 27.33943830702583 27.489036993777137 22.68423887493567 66-67 22.667669129150678 27.09740013409934 27.19176633490068 23.043164401849303 68-69 22.558745528951672 27.108189736005844 27.28026676006237 23.052797974980113 70-71 22.677345518162067 26.890556615010734 27.380884079428597 23.0512137873986 72-73 22.612008484394895 26.898820079962938 27.562123701929544 22.927047733712623 74-75 22.494264812796125 27.311779248170264 27.232441421452986 22.96151451758063 76-77 22.60652805168048 27.04336649280565 27.12582987880537 23.224275576708504 78-79 22.532981214023803 26.973007040673547 27.207253726282044 23.28675801902061 80-81 22.624439039429962 27.05281593641876 27.16333801514165 23.159407009009627 82-83 22.590771059450997 26.93086001928548 27.370435327707547 23.10793359355598 84-85 22.523807770397273 27.094574285980965 27.215700412145665 23.165917531476094 86-87 22.405550308231525 27.10840381540875 27.243830445687628 23.242215430672097 88-89 22.45290467215452 27.02932288397496 26.984494657006262 23.53327778686426 90-91 22.512033403237393 26.833697125598672 27.145910526798033 23.508358944365902 92-93 22.5596018465633 26.922497261924438 27.27363029857226 23.24427059294 94-95 22.40447991121699 26.85527632941168 27.278768204242027 23.4614755551293 96-97 22.176956321807584 26.84033358708879 27.448062196060768 23.534647895042863 98-99 22.317649305397968 26.861741527379472 27.126343669372343 23.694265497850214 100-101 22.149554158235507 26.732951358590707 27.214373119847647 23.903121363326143 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 23.0 1 18.5 2 36.5 3 58.5 4 47.0 5 41.0 6 71.5 7 160.0 8 265.5 9 330.5 10 326.5 11 300.5 12 326.5 13 355.0 14 407.0 15 475.0 16 524.5 17 587.0 18 647.5 19 723.5 20 835.5 21 1004.0 22 1214.5 23 1484.0 24 1913.5 25 2476.5 26 3338.5 27 4594.5 28 6455.0 29 9279.0 30 12591.0 31 16112.0 32 20101.5 33 23625.5 34 26398.5 35 28467.0 36 30247.0 37 31706.0 38 32225.5 39 32963.0 40 34221.5 41 35073.0 42 35722.5 43 37433.0 44 39827.0 45 42029.5 46 43479.0 47 46065.0 48 50386.5 49 50543.5 50 47013.5 51 45064.0 52 44013.5 53 42969.0 54 41621.0 55 39186.5 56 35876.5 57 32005.0 58 28277.5 59 24477.0 60 20290.5 61 16412.5 62 12611.0 63 9064.5 64 6409.0 65 4468.5 66 3065.5 67 2113.0 68 1478.0 69 1058.0 70 759.0 71 537.0 72 396.5 73 326.0 74 244.5 75 168.0 76 123.5 77 74.0 78 49.5 79 37.5 80 20.5 81 8.5 82 10.0 83 12.5 84 9.5 85 5.5 86 4.0 87 2.5 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.049580789713227796 2 0.0018838987455803307 3 5.137905669764538E-4 4 0.009933284294878109 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0023548734319754133 24-25 0.0 26-27 0.0 28-29 0.023677181961498248 30-31 0.020166279753825813 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.03301104392823716 50-51 0.010703970145342788 52-53 0.0 54-55 0.0012416605368597636 56-57 0.0 58-59 0.0 60-61 4.281588058137115E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 4.2815880581371154E-4 80-81 0.01293039593557409 82-83 0.006251118564880189 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1167791.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.27481983009902 #Duplication Level Percentage of deduplicated Percentage of total 1 76.50565294489556 50.70398364907037 2 14.371808520503132 19.049780406580542 3 4.547578823625143 9.0416990159679 4 1.868366965778265 4.953027361338532 5 0.9736134432866876 3.2263027768993777 6 0.537799933418234 2.1385556215159625 7 0.31259927718260444 1.4502222542007404 8 0.20964776443708344 1.1115494252680596 9 0.137569353354098 0.8205645696917464 >10 0.5178117552317614 5.45937068455387 >50 0.01210018040239085 0.5364880302286947 >100 0.004153176052973367 0.5526513121243661 >500 7.787170992497752E-4 0.3553302745828628 >1k 5.191447328331836E-4 0.6004746179769948 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2541 0.21759030511452818 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1712 0.1466015751106148 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1580 0.13529818263713284 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 8.56317611627423E-5 0.0 2 0.0 0.0 0.0 8.56317611627423E-5 0.0 3 0.0 0.0 0.0 8.56317611627423E-5 0.0 4 0.0 0.0 0.0 8.56317611627423E-5 0.0 5 0.0 0.0 0.0 8.56317611627423E-5 0.0 6 0.0 0.0 0.0 8.56317611627423E-5 0.0 7 0.0 0.0 0.0 8.56317611627423E-5 0.0 8 0.0 0.0 0.0 8.56317611627423E-5 0.0 9 0.0 0.0 0.0 8.56317611627423E-5 0.0 10-11 0.0 0.0 0.0 2.568952834882269E-4 0.0 12-13 0.0 0.0 0.0 3.853429252323404E-4 0.0 14-15 0.0 0.0 0.0 6.422382087205674E-4 0.0 16-17 0.0 0.0 0.0 0.001156028775697021 0.0 18-19 0.0 0.0 0.0 0.0014129240591852481 4.281588058137115E-5 20-21 0.0 0.0 0.0 0.0020979781484871864 8.56317611627423E-5 22-23 0.0 0.0 0.0 0.0038106133717420326 1.712635223254846E-4 24-25 0.0 0.0 0.0 0.005608880356159621 3.425270446509692E-4 26-27 0.0 0.0 0.0 0.0066364614901125284 3.425270446509692E-4 28-29 0.0 0.0 0.0 0.007963753788135035 3.425270446509692E-4 30-31 0.0 0.0 0.0 0.011260576592900612 3.853429252323404E-4 32-33 0.0 0.0 0.0 0.01738324751603669 4.2815880581371154E-4 34-35 0.0 0.0 0.0 0.02675992536335697 4.2815880581371154E-4 36-37 0.0 0.0 0.0 0.04148858828334864 4.2815880581371154E-4 38-39 0.0 0.0 0.0 0.06559392905066061 4.2815880581371154E-4 40-41 0.0 0.0 0.0 0.09492280724889984 4.2815880581371154E-4 42-43 0.0 0.0 0.0 0.12146865320934996 4.709746863950827E-4 44-45 0.0 0.0 0.0 0.15726272937537625 5.56606447557825E-4 46-47 0.0 0.0 0.0 0.20607283323813935 6.422382087205673E-4 48-49 0.0 0.0 0.0 0.2509866919679977 6.850540893019384E-4 50-51 0.0 0.0 0.0 0.30138098341227154 6.850540893019384E-4 52-53 0.0 0.0 0.0 0.35246032894584733 8.135017310460519E-4 54-55 0.0 0.0 0.0 0.4168982292208109 9.419493727901654E-4 56-57 0.0 0.0 0.0 0.4932817601779771 9.419493727901654E-4 58-59 0.0 0.0 0.0 0.5744606697602568 9.419493727901654E-4 60-61 0.0 0.0 0.0 0.6567099763570707 9.419493727901654E-4 62-63 0.0 0.0 0.0 0.7419563945945807 9.419493727901654E-4 64-65 0.0 0.0 0.0 0.8381636782609216 9.419493727901654E-4 66-67 0.0 0.0 0.0 0.9499131265783004 9.419493727901654E-4 68-69 0.0 0.0 0.0 1.0808440893961335 9.419493727901654E-4 70-71 0.0 0.0 0.0 1.2223077588369837 9.419493727901654E-4 72-73 0.0 0.0 0.0 1.3651415364564379 0.0011988446562783922 74-75 0.0 0.0 0.0 1.509816396940891 0.0011988446562783922 76-77 0.0 0.0 0.0 1.6690058409424289 0.0011988446562783922 78-79 0.0 0.0 0.0 1.8716962196146398 0.0011988446562783922 80-81 0.0 0.0 0.0 2.092026741086376 0.0012416605368597634 82-83 0.0 0.0 0.0 2.3141983454231108 0.0013701081786038768 84-85 0.0 0.0 0.0 2.535813343312288 0.0013701081786038768 86-87 0.0 0.0 0.0 2.780720180237731 0.0013701081786038768 88-89 0.0 0.0 0.0 3.0680575548193127 0.0013701081786038768 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 3175 0.0 28.137539 1 TACATGG 3135 0.0 28.03101 2 ACATGGG 3050 0.0 26.785269 3 CATGGGG 2050 0.0 23.632719 4 GTACCGT 145 2.3354743E-5 22.929651 6 CCGTAGA 150 3.0326762E-5 22.167227 2 TTGTACG 110 0.002878402 21.589605 9 TACCGTA 110 0.002878402 21.589605 7 GTATAGA 420 0.0 21.496923 1 CAGTACG 185 6.5556724E-6 20.539299 9 CCGACGT 70 4.8557768E-4 20.355915 12-13 TAGCGCA 120 0.004765208 19.790472 9 GGACCGT 170 7.957687E-5 19.557642 6 AGAGTAC 3520 0.0 19.228241 10-11 CTTACGC 125 0.006033303 18.998852 3 GTATAGC 455 0.0 18.798927 1 GAGTACT 2320 0.0 18.63034 12-13 CAGTGCG 255 2.6766065E-7 18.626326 9 GTACTAT 285 4.2522515E-8 18.340864 1 GCGTAAG 130 0.007545303 18.276737 1 >>END_MODULE